Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F7 I1 R1 17 60.9 2196714 94.7% 2080288 140.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 2,817,170 C→A E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA2,817,1700CA98.5% 209.4 / NA 68E112* (GAA→TAA) yqaBfructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (35/31);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGC  >  NC_000913_3_hsa_tpiA/2817026‑2817314
                                                                                                                                                |                                                                                                                                                 
tCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCCATGCCCCCGCGATGGCGCTTTAACTc                                                                                                                                               >  1:750439/1‑149 (MQ=255)
       cACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCCAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTc                                                                                                                                        <  1:955367/149‑1 (MQ=255)
       cACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTc                                                                                                                                        <  1:947568/149‑1 (MQ=255)
        aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTCTAACTCCCCGTTcc                                                                                                                                       >  2:1058335/1‑149 (MQ=255)
        aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCGTTAACTCCCCGTTcc                                                                                                                                       >  1:324715/1‑149 (MQ=255)
          gCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTa                                                                                                                                     >  2:1022830/1‑149 (MQ=255)
            ccATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACa                                                                                                                                   >  1:750622/1‑149 (MQ=255)
            ccATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCACGTTCCTACa                                                                                                                                   >  1:749058/1‑149 (MQ=255)
              aTACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑Gc                                                                                                                                >  1:675440/1‑149 (MQ=255)
              aTACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑Gc                                                                                                                                >  2:106626/1‑149 (MQ=255)
                  gCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCAtt                                                                                                                            >  1:765071/1‑149 (MQ=255)
                             aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGAcc                                                                                                                 >  2:447222/1‑149 (MQ=255)
                             aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGAcc                                                                                                                 >  1:1060381/1‑149 (MQ=255)
                                 aaaaTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCACTGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCCTTGGGCGACGACCATGc                                                                                                             >  2:545720/1‑149 (MQ=255)
                                     tgtgTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAAc                                                                                                         <  2:745718/149‑1 (MQ=255)
                                         tCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACtttt                                                                                                     <  2:675440/149‑1 (MQ=255)
                                           tGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGTCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTca                                                                                                   <  1:1040741/149‑1 (MQ=255)
                                               gcgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTcaccac                                                                                               <  2:605075/149‑1 (MQ=255)
                                                        tGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGACAGCAATGCCTAAGCGATGGCGCTTTAAATACCAGTAGCTACA‑GCCATTGAGCGACGCCCATGCCAACTTTTCCCCACGTCAACAag                                                                                      >  1:207730/1‑149 (MQ=255)
                                                         ggTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGGGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga                                                                                     <  1:1006841/149‑1 (MQ=255)
                                                          gtgtTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCCCATCAACAagag                                                                                    >  1:98621/1‑149 (MQ=255)
                                                           tgtTTGACGGGATCGGCAGCGACGACGTCGTCAAAAGAATGGCCTAATCCCATGTGTGCCAGCAATGCCTCAGCGCACGGGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATAAACAAGAgg                                                                                   <  2:553409/149‑1 (MQ=255)
                                                             tttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGaa                                                                                 >  1:649452/1‑149 (MQ=255)
                                                             tttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGaa                                                                                 >  1:795220/1‑149 (MQ=255)
                                                             tttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGaa                                                                                 <  1:340235/149‑1 (MQ=255)
                                                             cttGACGTTACCGGCAGCGACGTCGGCGTCAAAATAATGGCCTAATCCCAGGTGCGCCAGCAATTCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGaa                                                                                 <  1:532554/148‑1 (MQ=255)
                                                              ttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAg                                                                                <  1:578116/149‑1 (MQ=255)
                                                                 aCGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCgg                                                                             >  2:795221/1‑149 (MQ=255)
                                                                  cGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGt                                                                            >  1:690579/1‑149 (MQ=255)
                                                                   gTGATCGGCAGCGACGACGGCGTCAAGATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGtt                                                                           <  1:398932/149‑1 (MQ=255)
                                                                   gTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGtt                                                                           <  2:707640/149‑1 (MQ=255)
                                                                     gATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCg                                                                         >  2:639180/1‑149 (MQ=255)
                                                                      aTCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGa                                                                        >  2:988254/1‑149 (MQ=255)
                                                                       tCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCTCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTTGAc                                                                       >  2:522030/1‑149 (MQ=255)
                                                                          gCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACt                                                                                                        <  1:319832/113‑1 (MQ=255)
                                                                          gCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACt                                                                                                        >  2:319832/1‑113 (MQ=255)
                                                                           cAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTa                                                                   <  2:649452/149‑1 (MQ=255)
                                                                                acgacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcag                                                              <  1:795221/149‑1 (MQ=255)
                                                                                 cgacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagc                                                             >  2:430001/1‑149 (MQ=255)
                                                                                  gacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCAATTATTCAACAAGAGGAAGCGGTTCGACGCTATCcagca                                                            <  2:1060381/149‑1 (MQ=255)
                                                                                     ggCGTCAAAATAGTGGCGTAATCGCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAgcagca                                                         <  1:370865/149‑1 (MQ=255)
                                                                                          ccaactaatGCCGTAATCCCCGGTGCCCCAGCAACCCCTAAGCGATTGCGCTTTCACCCCCCGTTCCTACA‑CCCACTGGTCGCCGCCCATCCCAACTTTCCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActt                                                    <  2:320499/144‑1 (MQ=255)
                                                                                          cAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCACAACCATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCGACATActt                                                    >  2:62977/1‑149 (MQ=255)
                                                                                           aaaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCCGCAGCATActtc                                                   >  2:903850/1‑149 (MQ=255)
                                                                                           aaaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtc                                                   >  1:818310/1‑149 (MQ=255)
                                                                                           aaaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACCATCTCCAGCAGCATActtc                                                   >  2:223250/1‑149 (MQ=255)
                                                                                           aaaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCACCG‑TAATACATTCTATGCTGCGTCGACCGTGCCAACTTTTCACCACATCAAAAGGCGGAAGCGGTTCGACCCTATCCGCCAGCACAcctc                                                   >  2:276352/1‑149 (MQ=255)
                                                                                            aaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtct                                                  >  1:446403/1‑149 (MQ=255)
                                                                                               cacgtgCGTAATCCCTGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACTTCAACAGGGGGAAGGGGTTCGACGCTATCCAGCAGCATACTTCTTAc                                               <  2:33663/144‑1 (MQ=255)
                                                                                                aatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACt                                              <  2:582920/149‑1 (MQ=255)
                                                                                                aatGGACTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCGTCAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACt                                              <  2:309208/149‑1 (MQ=255)
                                                                                                 atGGCGGAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCAGGCCAACTTTGCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTg                                             <  2:750439/149‑1 (MQ=255)
                                                                                                     cGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACg                                                                      >  2:348814/1‑120 (MQ=255)
                                                                                                     cGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACg                                                                      <  1:348814/120‑1 (MQ=255)
                                                                                                     cGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTc                                         <  1:106626/149‑1 (MQ=255)
                                                                                                           cccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGtttt                                   <  2:818310/149‑1 (MQ=255)
                                                                                                                gTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAc                              <  1:639180/149‑1 (MQ=255)
                                                                                                                  gcgcCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTGCA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAcgc                            <  1:831979/149‑1 (MQ=255)
                                                                                                                              ccTCAGGGGTGGCGGTTTGACGCGGCGGTGCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGc                <  2:173838/149‑1 (MQ=255)
                                                                                                                              ccTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGc                >  1:641745/1‑149 (MQ=255)
                                                                                                                               cTCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCg               >  1:577215/1‑149 (MQ=255)
                                                                                                                                tCAGCGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCgg              >  1:953372/1‑149 (MQ=255)
                                                                                                                                    cGATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCg          >  1:238521/1‑149 (MQ=255)
                                                                                                                                     gATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACTCGCTAACGCATGCGGGTCga         >  2:321603/1‑149 (MQ=255)
                                                                                                                                     gATGGCGCTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCga         <  2:446403/149‑1 (MQ=255)
                                                                                                                                          cgcTTTAACTCCCCGTTCCTACA‑GACATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCg    <  1:54600/149‑1 (MQ=255)
                                                                                                                                            cTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGc  <  1:545720/149‑1 (MQ=255)
                                                                                                                                            cTTTAACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCGATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGc  <  1:430001/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACA‑GCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGC  >  NC_000913_3_hsa_tpiA/2817026‑2817314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A