Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F9 I0 R1
|
23 |
38.9 |
1378630 |
88.6% |
1221466 |
148.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913_3_hsa_tpiA |
2,817,170 |
C→A |
100% |
E112* (GAA→TAA) |
yqaB ← |
fructose‑1‑P and 6‑phosphogluconate phosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_hsa_tpiA | 2,817,170 | 0 | C | A | 99.0%
| 91.6
/ ‑6.7
| 32 | E112* (GAA→TAA) | yqaB | fructose‑1‑P and 6‑phosphogluconate phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (16/15); minor base T (1/0); total (17/15) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGC > NC_000913_3_hsa_tpiA/2817033‑2817314
|
cACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTc > 1:494260/1‑149 (MQ=255)
gCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTa > 1:411364/1‑149 (MQ=255)
tACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGcc > 1:247046/1‑149 (MQ=255)
ggcgcgcACAACCAAAAGGGTGCTGGCGGGATTTTATGGTGTTTGCCGTGTGCGGCAGGGAAGAGGGCGTTAAAATAAAGGTGTTTTCCTAGGTGCGCCAGCAATGCCACCGCGACGGCGTTGTAACTCCCCGTTCCTACAGCCATTgg < 2:397284/148‑1 (MQ=255)
aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACTAcc > 2:247454/1‑149 (MQ=255)
tgtCTGGCGCGGGTTTTGGNTNTNTNACGTGATCGGCAGCGACGACGGCGGGAAAATAATGGCGTAATCCCAGGTGCCCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACtt < 1:495859/149‑1 (MQ=255)
tGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca > 2:250803/1‑149 (MQ=255)
cgGGCTTATGGTGTCTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACcaca < 1:50391/149‑1 (MQ=255)
ggTGTATGGTGTTTCACGTGATAGACCGAGAAGACGGACTACAAATAATAACGTATTCCGAGGTTTGCCAGCAATGCCTCTGCGATGGCGCTTTTACCCCCCGTTTCTAGAACCATTGGGCCACGACCATGCCAACTTTTCACAACAtg > 2:306312/1‑148 (MQ=255)
ggTATTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga < 1:188035/149‑1 (MQ=255)
gtgtTTGACGTTATCGGCAGCGACGACGGCGAAAAAATAATGGAGTAATCCCAAGTGCGCCAGCAATGCCTCAGCGATGACGCTTTAACTCCCAGTTCCTACAGCCATGTGCCGCCGAACATCCAAACCATTCCCCACCTCACCAagag > 2:268158/1‑149 (MQ=255)
gtTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAACAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCACCAAGAGGa > 2:53659/1‑149 (MQ=255)
gACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCg > 2:481456/1‑149 (MQ=255)
aCGTTATCGGCAGCGACGACGGCATCAAAATAATGGCGTAATCCAAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCACGTGCCTACAGCCATTGGGCGACGAGCATGCACAATTTTAACCAAAGCAAAAACACGAAGAgg > 2:635920/1‑149 (MQ=255)
acgacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcag < 1:392622/149‑1 (MQ=255)
gacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagca < 1:47954/149‑1 (MQ=255)
cAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActt < 1:638719/149‑1 (MQ=255)
aaaaTAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCAAACTTTTCACCACATCAACAAGCGGAAGCGGTTCGACGCTCTCCACCACCTTActtc > 2:600403/1‑149 (MQ=255)
aataatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTCTCCAGCAGCATActtctt > 2:84267/1‑149 (MQ=255)
ataatGGTGTATGCCGAGTTGCCCCAGCACCTGCTCAGGGGTGGCGCTGTAACTCCTCGTCCCCACACCCATTGGGCGACGACCATGCGAACTTTTCAACACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTa < 2:498338/149‑1 (MQ=255)
ataatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTa < 1:38672/149‑1 (MQ=255)
taatGGCGAAATCCCCGGTGCGCCAGCAATCCCGTAGCGGTGCCCCTTTAACTCCCCGTTCCTCCAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTAc < 1:187477/149‑1 (MQ=255)
tGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGc < 2:555226/149‑1 (MQ=255)
gCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAGGAGGAAGCGGTTCGGCGCTATCCAGCAGCATACTTCTTCCTGCtt > 1:338182/1‑149 (MQ=255)
gTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCt < 1:602873/149‑1 (MQ=255)
ccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGttttt > 1:274787/1‑149 (MQ=255)
aGGTGCGTCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTc < 1:467508/149‑1 (MQ=255)
ccAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAACCATTGGGCGACGACAATGCCCACCTTCCACCACATCAACAAAAGGAAGCGGTTCAACGCAACCCAGCCGCACACTTCTTACACCTTCAGTTTTTTCCCCCGCt > 2:124727/1‑149 (MQ=255)
tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAAGTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATATTTCTTACTGCTTCTGTTTTTTCACGCGATAACGCATgggg > 1:456714/1‑149 (MQ=255)
gCGATGGCGTTTTAACCCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCGCGCGCTAACGCATGCGGGTc < 2:422149/149‑1 (MQ=255)
tGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGAAGCATACGCCGTACTGCTTCTGTTTTTTCAAGGGCTAAGGCATGCGGGCAgaga > 1:470829/1‑149 (MQ=255)
cTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGc < 1:468807/149‑1 (MQ=255)
|
CACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGC > NC_000913_3_hsa_tpiA/2817033‑2817314
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
