Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F76 I1 R1	22	48.9	1690474	94.9%	1604259	142.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	2,817,170	C→A	E112* (GAA→TAA)	yqaB ←	fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	2,817,170	0	C	A	95.6%	132.8 / ‑0.5	45	E112* (GAA→TAA)	yqaB	fructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (15/28); total (16/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01

GGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAT  >  NC_000913_3_hsa_tpiA/2817028‑2817310

ggTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTccc                                                                                                                                        <  1:41699/149‑1 (MQ=255)

  ttGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATACTGACGTAAGCCCAGGTGCGCCAGCAAT‑GCCTGAGCGATGGCGCTTTAACTCCCCg                                                                                                                                      >  1:238545/1‑149 (MQ=255)

      aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTcc                                                                                                                                  <  1:228302/149‑1 (MQ=255)

                ggtGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCAtt                                                                                                                        <  2:490599/147‑1 (MQ=255)

                gCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGAAGTGATCAACAGCAACGACAGCGTCAAAATAATGGCGTGAGCCAAGG‑AAGCTAGTATTCGCCTCAACGATGACGCTTTATATCCCAGTTCCTAAAGCCaat                                                                                                                        >  1:538758/1‑147 (MQ=255)

                gCTGCGCGCACAACAAAAAGGTGTCTGGCGCGGGTTTATGGTGTTTGATGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCAtt                                                                                                                        <  1:164932/149‑1 (MQ=255)

                  tGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTgg                                                                                                                      <  1:360330/149‑1 (MQ=255)

                    cgcgcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGc                                                                                                                    <  2:591790/149‑1 (MQ=255)

                       gcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgac                                                                                                                 <  1:276953/149‑1 (MQ=255)

                         acaacaAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgacga                                                                                                               >  1:782990/1‑149 (MQ=255)

                           aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTCCAGCCATTGGGCGACGAcc                                                                                                             >  1:324071/1‑149 (MQ=255)

                             caaAAATGTGTCTGGCGCGGTTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCAt                                                                                                           <  1:323580/149‑1 (MQ=255)

                             caaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTGCCTACAGCCATTGGGCGACGACCAt                                                                                                           >  1:608573/1‑149 (MQ=255)

                                     tgtCTTGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAAGAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACtt                                                                                                   <  1:569636/149‑1 (MQ=255)

                                         tGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                                                               <  2:426712/149‑1 (MQ=255)

                                         tGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                                                               <  1:357151/149‑1 (MQ=255)

                                         tGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca                                                                                               <  1:233540/149‑1 (MQ=255)

                                          ggCGCGGGTTTATGGTGGTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTcac                                                                                              <  1:322349/149‑1 (MQ=255)

                                           gcgcGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTc                                                                                                                                   <  1:681546/111‑1 (MQ=255)

                                           gcgcGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTc                                                                                                                                   >  2:681546/1‑111 (MQ=255)

                                                     aTGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATcaacaa                                                                                   >  2:466194/1‑149 (MQ=255)

                                                      tGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAag                                                                                  <  1:313823/149‑1 (MQ=255)

                                                       ggTGTTTGCCGGCATCGACAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga                                                                                 <  1:143328/149‑1 (MQ=255)

                                                       ggTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga                                                                                 >  2:511712/1‑149 (MQ=255)

                                                       ggTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga                                                                                 <  1:334560/149‑1 (MQ=255)

                                                              gACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCg                                                                          >  1:229621/1‑149 (MQ=255)

                                                                ggTGAACGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAG‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGt                                                                        <  2:682351/148‑1 (MQ=255)

                                                                      cGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACg                                                                  <  1:511712/149‑1 (MQ=255)

                                                                      cGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACg                                                                  >  1:7255/1‑149 (MQ=255)

                                                                         cAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTACGCCGCTa                                                               >  2:417115/1‑149 (MQ=255)

                                                                           gCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGCCGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATc                                                             >  2:680082/1‑149 (MQ=255)

                                                                                gacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagca                                                        <  1:466194/149‑1 (MQ=255)

                                                                                gacgGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagca                                                        <  1:317615/149‑1 (MQ=255)

                                                                                gacgGCGTCAAAATAATGCCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagca                                                        <  1:153334/149‑1 (MQ=255)

                                                                                                tGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGc                                        <  2:7255/149‑1 (MQ=255)

                                                                                                 ggCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCt                                       <  2:111988/149‑1 (MQ=255)

                                                                                                  gCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCGGCAGCATACTTCTTAc                                           >  2:844902/1‑144 (MQ=255)

                                                                                                  gCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTAc                                           <  1:844902/144‑1 (MQ=255)

                                                                                                  gCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCtt                                      <  1:638734/149‑1 (MQ=255)

                                                                                                   cGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTc                                     <  2:760803/149‑1 (MQ=255)

                                                                                                                        aaT‑GCCTCAGCGATGGCGCTTTAAATCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGc                >  2:17050/1‑149 (MQ=255)

                                                                                                                             ccTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCCCATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATCCGTCTTTCTGCTTCTGTTTTTTCACCCGCTAACCCAttc            >  1:219603/1‑147 (MQ=255)

                                                                                                                               tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCgg          >  1:13024/1‑149 (MQ=255)

                                                                                                                                  gCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGAAGCCATCCAGCAGCATCCTTCTTACTGCTTCTGTTTTTTCAAGCGCTAACGCATGCGGGTc       <  2:711322/149‑1 (MQ=255)

                                                                                                                                       ggCGCGTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAt  <  2:296834/149‑1 (MQ=255)

GGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT‑GCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAT  >  NC_000913_3_hsa_tpiA/2817028‑2817310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A