Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F29 I0 R1
|
32 |
37.4 |
1321382 |
89.2% |
1178672 |
148.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913_3_hsa_tpiA |
2,817,170 |
C→A |
100% |
E112* (GAA→TAA) |
yqaB ← |
fructose‑1‑P and 6‑phosphogluconate phosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_hsa_tpiA | 2,817,170 | 0 | C | A | 100.0%
| 96.8
/ NA
| 33 | E112* (GAA→TAA) | yqaB | fructose‑1‑P and 6‑phosphogluconate phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (12/21); total (12/21) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCC > NC_000913_3_hsa_tpiA/2817026‑2817315
|
tCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGACAGAAATGCCTCCGGGATGCCGCTTTAACTc > 2:349735/1‑149 (MQ=255)
gTTGCACGCCCATCCGCCGCGCGCAAAACAAAAATGTGTCTGGCGCGGGTTTATGGTGGTTGACGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTcccc < 1:231650/149‑1 (MQ=255)
gTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTcccc < 1:629451/149‑1 (MQ=255)
gCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTa < 1:226341/149‑1 (MQ=255)
ccATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACa < 1:378128/149‑1 (MQ=255)
ccATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTGTATGGTGTTTGACGTGATCGGCAGCGA‑CGACGGCGTCAACATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCACCGATGGCGCTTTAACTCGCCGTTCCTACa > 2:445057/1‑149 (MQ=255)
gcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCAAGGTGTGCCTGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgac > 1:647786/1‑149 (MQ=255)
gcACAACAAAAATGTGTCTGGCGCGGGGTTATGGTGTTTGACGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgac < 1:630445/149‑1 (MQ=255)
acaacaAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgacga < 1:559929/149‑1 (MQ=255)
aacaaAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGAcc > 1:443594/1‑149 (MQ=255)
aaaTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGGTCGGCAGCGA‑CGACGGCGGCAAAATAATGGCGTAATCCCAGGTGCGCCAGTAATGCCTCAGCGAGGGCGCTTTAACTCCCCGTTCCTACAGCCATTGTGGGACTCGCATGcc > 2:221044/1‑149 (MQ=255)
tttATGGTGTTTGACGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATcaa < 1:535108/149‑1 (MQ=255)
gcggTGATCGGCAGGGAGCGA‑GGCGTCAAAAGAAGGGCTTAATCCCAGGTGAGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCg < 2:87229/146‑1 (MQ=255)
aCGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCgg < 2:149938/149‑1 (MQ=255)
aCGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCCTCATCCCGGTTTCACCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACATCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCgg < 1:122497/149‑1 (MQ=255)
gTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAAGCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACCCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGtt < 2:461863/149‑1 (MQ=255)
ttaTCAGCAGTGG‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCTCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACTTCAACAAGAGGAAGCGGTTc < 2:247958/147‑1 (MQ=255)
aTCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGa < 1:265117/149‑1 (MQ=255)
cAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTa < 1:494695/149‑1 (MQ=255)
tacgGCGTCAAAATAATTGTGTTATTCTAGGTGTGTCAGTATTGCTTCAGCGATGGCTTTTTATCTTTCCGTTCTTTCAGTCATTGGGCGACGACCATTTCAACTTTTCACCTCATTAATAATAGTAAGCGGTTCGACGCTATCcagca < 2:265461/148‑1 (MQ=255)
gacgGCGTCAAAATAATGGCGTACTCCCAGGTGCGCCGGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCCTGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagca < 2:143490/149‑1 (MQ=255)
ggCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCCCCACACCAACCACAGCAAGCGATTCGACCCTATCCAgcacca > 2:306838/1‑149 (MQ=255)
gCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCAt > 1:599998/1‑149 (MQ=255)
aataatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtctt < 1:188225/149‑1 (MQ=255)
ataatGGCGCAATCCCAGGTGCGCCAGCAATGCCTCAGGGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTa < 2:576262/149‑1 (MQ=255)
taatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTAc > 1:207850/1‑149 (MQ=255)
aatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTCTCCAGCAGCATACTTCTTACt > 1:396275/1‑149 (MQ=255)
gCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGGCATTGCGCGGCGGACATGCCAACTTTTCACCACATCAGCAAGACGAAGCGTTTCGAGACTATCCACCACCAGGCTTCTTACTGCtt > 2:452030/1‑149 (MQ=255)
ccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGtttat < 2:611047/149‑3 (MQ=255)
cgcCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAcgcg > 2:1310/1‑149 (MQ=255)
tCAGCGGTGGCGCTTGAATCCCCCGTTCTTAGAGCCATTGGGCTACGACCATGCCAACTTTTCACCACATCGACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTGACTGCTTCTGTTTTTTCACGCGCTAACGCATGCgg < 2:457228/149‑1 (MQ=255)
tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTACCGCATGCgg > 2:281729/1‑149 (MQ=255)
tttAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGcc < 1:566085/149‑1 (MQ=255)
|
TCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGA‑CGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCC > NC_000913_3_hsa_tpiA/2817026‑2817315
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
