Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F101 I1 R1
|
22 |
40.0 |
1400210 |
94.5% |
1323198 |
141.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_hsa_tpiA |
2,817,170 |
C→A |
E112* (GAA→TAA) |
yqaB ← |
fructose‑1‑P and 6‑phosphogluconate phosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_hsa_tpiA | 2,817,170 | 0 | C | A | 100.0%
| 67.6
/ NA
| 23 | E112* (GAA→TAA) | yqaB | fructose‑1‑P and 6‑phosphogluconate phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (9/14); total (9/14) |
CGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCT > NC_000913_3_hsa_tpiA/2817043‑2817316
|
cGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCAtt < 1:27980/150‑1 (MQ=255)
cTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTgg < 1:496979/150‑1 (MQ=255)
ggcgcgcACAACAAAAAGGTGTCTGGAGCGGGGTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTggg < 2:636533/149‑1 (MQ=255)
aTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCCACG‑ACCATGCCAAc > 2:372837/1‑150 (MQ=255)
gtCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACtttt < 1:571262/150‑1 (MQ=255)
tCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTc < 1:424814/150‑1 (MQ=255)
cgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACAt > 2:561056/1‑150 (MQ=255)
gggTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATc < 2:86062/150‑1 (MQ=255)
tGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACCaga > 1:79294/1‑150 (MQ=255)
ggTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAagag > 1:697246/1‑150 (MQ=255)
ggTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAagag < 1:157264/150‑1 (MQ=255)
aTCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGAc < 2:133470/150‑1 (MQ=255)
aTCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCA‑TCGTTTACGCGCCGTGCCACCTTTTCACCACATCAACAAGAGGAAGCGGTTCGAc > 1:159068/1‑150 (MQ=255)
cgagtgCGTGAAAATAAGGGCGTGAGCCCATGGCCGCCATCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCcagca < 2:563879/145‑1 (MQ=255)
aagaatGGCGTAATCCTAGGTGCGCCAGCAATGTCTCAGCGAGGGCGCTTTACCTCCCCGTTCCTACAGCCATTGGGTGACG‑ACCATGCCAACGTTCCACCGCATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATActtcttc < 2:497081/147‑2 (MQ=255)
agaatGGCGAAATCCCAGGGGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTTCTACAGCCATTGGGCGACG‑ACCATGCCAACCTTTCTCCACATCAACAAGAGTAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTAc < 2:289798/148‑1 (MQ=255)
aatGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACACGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTg < 1:518408/150‑1 (MQ=255)
ggCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCGCTCCGTCGACAAGAGGAAGCGGGTGGACGCTATCCAGCAGCATACTTCTTACTGCtt > 1:512836/1‑150 (MQ=255)
gCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTCTCCCGCAGCATACTTCTTACTGCTTc > 1:88576/1‑150 (MQ=255)
cccAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGttttt < 1:674052/150‑1 (MQ=255)
gTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCCCATCAACAAGATGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCAcg < 2:79294/150‑1 (MQ=255)
tCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATCCTTCTTACTGCTTCTGTTTTTTCACGCCCTAACCCCTGCggg > 2:262045/1‑150 (MQ=255)
tttAACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCt > 1:269344/1‑150 (MQ=255)
|
CGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACG‑ACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCT > NC_000913_3_hsa_tpiA/2817043‑2817316
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
