Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F58 I0 R3
|
223 |
82.2 |
2922920 |
95.2% |
2782619 |
126.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP006881 |
2,801,996 |
G→A |
100% |
A327T (GCC→ACC) |
U712_14440 → |
UPF0118 membrane protein ytvI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 2,801,996 | 0 | G | A | 93.4%
| 155.8
/ ‑4.5
| 57 | A327T (GCC→ACC) | U712_14440 | UPF0118 membrane protein ytvI |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/30); minor base C (5/0); total (27/30) |
| Fisher's exact test for biased strand distribution p-value = 1.93e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGCAAA > CP006881/2801861‑2802128
|
ttATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCAccc > 3:293923/1‑139 (MQ=255)
ttACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGCCAGAGCCCCAGCTACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc > 5:274890/1‑139 (MQ=255)
ttACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACCCTCATCCCCCTGGTTGc > 6:272959/1‑139 (MQ=255)
ttACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGAAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc < 3:294369/139‑1 (MQ=255)
ggCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCt < 4:298426/139‑1 (MQ=255)
cAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTa < 5:178856/139‑1 (MQ=255)
ccccAAGCAATCGGAATTGGTATCCTGTACATCGTCGTTGTTATTCAGCGGCAGATGGCAGCGCCCAAGATACTAAGTAAAAGATTCCGGATCGATCCGCTGGCAACACACAACAACCTTTTTGCGCGCGTTAaataat > 6:221914/1‑139 (MQ=255)
ccAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTAAGCGGCAGCTGACAGCGCCCAAGATACTAAGTAAATCTATAGGGATCGATCCCCTGGCAACACTCAAAACCCTGTTTGCCGGCTTTAAATTATTc > 8:214539/1‑139 (MQ=255)
ccAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCGTATTCAGCGGCCGCTGACAGAGCCCCAGATACTAAGTAAATGTATCGGGATGGATCCGCTGGCACCACTCATCACCCAGTTTGCCGGCGTTAAagtattg > 1:316536/1‑138 (MQ=255)
tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCggg < 5:272959/137‑1 (MQ=255)
tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc < 4:293923/139‑1 (MQ=255)
tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc < 6:321154/139‑1 (MQ=255)
cTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTTAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCgg < 5:221914/139‑1 (MQ=255)
cgtcgtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCCGGTTTTTAGGACTGATTGAGGGACCGGcg > 7:355761/1‑138 (MQ=255)
cgtcgtTCTTATTAAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATAGGGATCGCTCCGCTGTCCAAAAACAAACCCCTTGTTGGCGGCTTTAAATTATTCGGGTTTTTTGTACTTATTGCGGGACCggat > 8:150700/1‑137 (MQ=255)
tcgtggttattaAGCCGCCGCGGGCAGGGCCCCAGATTCTAAGTAAATCTATCGGGATAGATCCCCGGGCACCACTCATCCCCCGGTTTGCCGGCTGTAAATATTTCGGGTTTTTTTGACAGATTGCGGGGCCCGgtgt > 8:161900/7‑139 (MQ=255)
tcgtTCTTATTCAGCGGCAGCGGACAGAGCCCAAGATTCTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTTGGACTGATTGCGGGACCGGCTg > 6:349354/1‑138 (MQ=255)
gtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTAc < 7:122639/139‑1 (MQ=255)
gtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTAc < 7:214539/139‑1 (MQ=255)
aTTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtc < 5:268900/138‑1 (MQ=255)
aTTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtc < 1:126752/138‑1 (MQ=255)
cGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCCACACTCATCCCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCatcatc > 1:127774/1‑138 (MQ=255)
gCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGTCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCt < 1:197464/139‑1 (MQ=255)
agCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc > 8:348971/1‑64 (MQ=255)
agCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc < 7:348971/64‑1 (MQ=255)
ccAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg > 4:225763/1‑79 (MQ=255)
ccAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg < 3:225763/79‑1 (MQ=255)
aGATACTAAGTAAATCTATCGGTATCGATCCGCTTGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAGCGGCTGTACTCGTGATCATCGTACCTTTCTTCTCAACAgg < 1:39938/139‑1 (MQ=255)
aGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCTTCACAACAgg < 5:236720/139‑1 (MQ=255)
aGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAgg < 1:284514/139‑1 (MQ=255)
tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAgg < 8:13659/133‑1 (MQ=255)
tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAgg > 7:13659/1‑133 (MQ=255)
tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAt > 1:207373/1‑138 (MQ=255)
aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTc > 7:322241/1‑61 (MQ=255)
aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTc < 8:322241/61‑1 (MQ=255)
aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCCTCCACGCTTTCATCACACCAGGCGCATTAAAAGa > 1:91557/1‑138 (MQ=255)
aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCACGCTTTCATCACAACAGGCGCATTAAAAGa > 5:184174/1‑138 (MQ=255)
aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGa > 7:141343/1‑138 (MQ=255)
tATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTATGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 7:150700/139‑1 (MQ=255)
tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc > 2:328966/1‑40 (MQ=255)
tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc < 1:328966/40‑1 (MQ=255)
tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACt < 6:201550/91‑1 (MQ=255)
tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACt > 5:201550/1‑91 (MQ=255)
tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTTATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATg > 1:260335/1‑139 (MQ=255)
tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 6:274890/137‑1 (MQ=255)
gtgatcgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg < 1:239411/136‑1 (MQ=255)
gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 3:285924/135‑1 (MQ=255)
gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata > 4:285924/1‑135 (MQ=255)
ggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTc < 7:161900/139‑1 (MQ=255)
cgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCTGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCATTCATCACAACAGGCGCATTAAAAGAAATATGGTCCta > 2:61969/1‑138 (MQ=255)
atcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTatat < 8:241195/139‑1 (MQ=255)
tcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTtcat > 5:127484/1‑102 (MQ=255)
tcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTtcat < 6:127484/102‑1 (MQ=255)
tcCCCTGGCAACACTCATCCCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTCCGGGACCGGCTGTACTCGTCATCATCCACGCATTCAACACAACACGCGCATTAAAAGAAATATGGTCCTatat > 6:99224/1‑138 (MQ=255)
acacTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGc < 2:181037/139‑1 (MQ=255)
tcatcaCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGCaaa > 3:206868/1‑138 (MQ=255)
cATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg > 6:355314/1‑115 (MQ=255)
cATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg < 5:355314/115‑1 (MQ=255)
|
TTATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATATCACCGTTCAGCAAA > CP006881/2801861‑2802128
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
