Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A17 F104 I0 R1 40 45.6 1767846 90.1% 1592829 142.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,341,824 T→G 100% intergenic (‑196/‑87) melR ← / → melA DNA‑binding transcriptional dual regulator MelR/alpha‑galactosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,341,8240TG100.0% 149.6 / ‑6.7 53intergenic (‑196/‑87)melR/melADNA‑binding transcriptional dual regulator MelR/alpha‑galactosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (27/25);  minor base C (0/1);  total (27/26)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTC  >  NC_000913/4341684‑4341961
                                                                                                                                            |                                                                                                                                         
tGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTACGAGTATAATAAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTa                                                                                                                                         >  1:330599/1‑143 (MQ=255)
  cgggTGGGAGCGCGGTTTTCACCCTCTGCCCAGAGGGGCGCGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATg                                                                                                                                       <  2:473397/140‑1 (MQ=255)
      tGGAAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACGCAGATTAACTGCTGCTTCACGCAGGATCTGAGTGTATGGGaa                                                                                                                                   <  1:47490/143‑1 (MQ=255)
       gggAGGACGGTTTTGACCCTCTGCCCAGAGGGGCGAGGGGCCTCTCCGAGTATCATGAGGCCGGAGACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAAt                                                                                                                                  <  2:503977/143‑1 (MQ=255)
         gAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGTATCTGAGTGTATGGGAATGc                                                                                                                                >  2:570181/1‑143 (MQ=255)
              cGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATAAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAGAGTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAAc                                                                                                                           >  1:121838/1‑143 (MQ=255)
              cGGTTTTCACCCTCTTCCCAGAAGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTACCATAAACTCAGATTTACTGCTGCTTCACGCAGGCTCAGAGTGTCTGAGAATGCTCAAc                                                                                                                           >  2:500258/1‑143 (MQ=255)
                  tttCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGGGTATGGGAATGCTCAACCTgg                                                                                                                       >  1:83350/1‑143 (MQ=255)
                    tCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTggga                                                                                                                     >  2:242360/1‑141 (MQ=255)
                     cACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAg                                                                                                                    >  1:459067/1‑143 (MQ=255)
                        ccTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCCTGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGGTCTGAGTGTGTGGGACTGCTCGACCTGGAAGCCg                                                                                                                 >  1:407034/1‑143 (MQ=255)
                        ccTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCg                                                                                                                 >  1:472963/1‑143 (MQ=255)
                        ccTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCCGCCTCACTTAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCg                                                                                                                 <  1:453770/143‑1 (MQ=255)
                          tctTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCCTGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTACTATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGGATGCTCAACCTGGAAGCCGGa                                                                                                               >  2:477952/1‑143 (MQ=255)
                           ctTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGTAAACTCTGCTTTTCAGGTACTTTTTTCCTATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAg                                                                                                              <  2:715004/143‑1 (MQ=255)
                            ttCCCCGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGCGCGATCTACTTTGCAGGTAATTTATCCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAgg                                                                                                             <  1:369298/143‑1 (MQ=255)
                                cgggggggCGAGGGGACTCCCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTCATTTATTCCCATAAACTCAGATTTACTGCTGCTTCCCGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGtttt                                                                                                         <  2:375983/139‑1 (MQ=255)
                                cAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGtttt                                                                                                         >  1:782888/1‑143 (MQ=255)
                                 agagGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTc                                                                                                        <  1:307879/143‑1 (MQ=255)
                                   agGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTg                                                                                                      <  1:513882/143‑1 (MQ=255)
                                     gggCGAGGGGACTCTCCGAGTATCATGAGGCCGAAACCTCTGCTTTTCAGGTAATTTATTCCCATAAACTCCGATTGACTGCTGCTTCACGCAGGAGCCGCCGGTGTGGGAAGGCTCCACCTGCACGCCGGCGGCTTTCTGCa                                                                                                    >  2:446918/1‑143 (MQ=255)
                                              gACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTGCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCt                                                                                           <  2:374880/143‑1 (MQ=255)
                                                 tctcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGcc                                                                                        >  1:8193/1‑143 (MQ=255)
                                                  ctcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTGTGCAGATTCGCCTGCCa                                                                                       >  2:55980/1‑143 (MQ=255)
                                                   tcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCat                                                                                      >  1:358859/1‑143 (MQ=255)
                                                        gTATCATGACGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGCTCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCAtgatga                                                                                 <  2:427438/143‑1 (MQ=255)
                                                                   ccGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGTTCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGCGGTTTTCTGCAGATTCGCCTGCCATGATGCAGTTATTcacg                                                                      >  2:293384/1‑141 (MQ=255)
                                                                     gAAAACTCTGCTTTTCAGGAAATTTATTCCAATAAACTCAGATTTACTGCCGCTTCACGCAGGATCTCAGCGTATGGGAATGCTCCACCTGGAAACCGGAGGTGTTCTGCAGATTCGCCTGCCATGATGACGATATTcacgca                                                                    >  2:454337/1‑143 (MQ=255)
                                                                             tGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGga                                                            >  2:366282/1‑143 (MQ=255)
                                                                                ttttCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGAt                                                         >  2:147585/1‑143 (MQ=255)
                                                                                 tttCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGgagacc                                                        >  1:79873/1‑141 (MQ=255)
                                                                                     aGGTAATTTAGTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCa                                                    <  1:819959/143‑1 (MQ=255)
                                                                                      ggTAATTTATTCCCATGAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCat                                                   <  1:574821/143‑1 (MQ=255)
                                                                                        tAATTTATTCCCATAACCTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCatga                                                 <  2:546701/143‑1 (MQ=255)
                                                                                          aTTTATTCCGATAAACTCAGATTTACTGCTGCTTGACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCatgatg                                               <  2:89026/143‑1 (MQ=255)
                                                                                           tttATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGt                                              >  2:533610/1‑143 (MQ=255)
                                                                                            ttATTCCCATGAACTCTGGTTTACTGCTGCTCAGACCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTc                                             <  2:180622/143‑1 (MQ=255)
                                                                                             tATTCCCAGAAGCTCCGATTTACTGCTGCGTCACGCAGGAGCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCt                                            <  1:714806/143‑1 (MQ=255)
                                                                                                 cccATGAGCTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCAc                                        <  2:353949/143‑1 (MQ=255)
                                                                                                      aaaGTCAGGTGTACCGCTGCGTCACCCAGGATCTGAGTCTATCGGAATGCTCAACCTGGAAGCCGGAGCTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCaaa                                   <  2:335577/143‑1 (MQ=255)
                                                                                                          tCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTa                               >  1:471725/1‑143 (MQ=255)
                                                                                                          tCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCGGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTa                               <  2:653088/143‑1 (MQ=255)
                                                                                                          tCAGATTTACTGCTGCTTCACGCAGGATATGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCAATGATGAAGTTATTAAAGCAAGACAGGAGAGCTGAATGATGTCTGCGCCCaaaaaga                               >  2:283171/1‑140 (MQ=255)
                                                                                                            aGATTTACTGCTGCTGCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACa                             <  2:337257/143‑1 (MQ=255)
                                                                                                              aTTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACAtt                           <  2:690332/143‑1 (MQ=255)
                                                                                                               tttACTGCTGCTTCACGCAGGATCTGAGTGTATGGAAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCAGGATGAAGTTATGCAAGCAAGCCAGGAGATCTGCATGATGGCTGCACCCCAACTTACCttt                          >  1:643607/1‑143 (MQ=255)
                                                                                                                 tACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTAt                        <  2:472963/143‑1 (MQ=255)
                                                                                                                     gctgctTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGACGGAGGTTTTCTGCAGATTCGCCGGCCATGATGAAGGAATTCAAACAACCCAGGATTTGTGCATGATGCCTGCACCAAAAGTGACATTGATCGgc                    >  2:855292/1‑143 (MQ=255)
                                                                                                                         ctTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCACCGAAATATTTTAGAGGTCGGCACCCAAAATTACATTTATCGGCGCTg                <  1:604919/143‑1 (MQ=255)
                                                                                                                           tCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGt              >  1:719039/1‑143 (MQ=255)
                                                                                                                               gCAGGATCTGGGTGGATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTcga          <  2:121838/143‑1 (MQ=255)
                                                                                                                               gCAGGATACGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGGTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAACATTACATTTATCGGCGCTGGTTcga          <  1:719481/143‑1 (MQ=255)
                                                                                                                                       tGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTc  >  2:540943/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTC  >  NC_000913/4341684‑4341961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A