Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F104 I1 R1	25	31.6	1325236	90.2%	1195362	142.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,341,824	T→G	intergenic (‑196/‑87)	melR ← / → melA	DNA‑binding transcriptional dual regulator MelR/alpha‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,341,824	0	T	G	100.0%	130.5 / NA	41	intergenic (‑196/‑87)	melR/melA	DNA‑binding transcriptional dual regulator MelR/alpha‑galactosidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (19/22); total (19/22)

CGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTT  >  NC_000913/4341686‑4341960

cGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGACAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGGTCTGAGTGTATg                                                                                                                                      >  1:638157/1‑143 (MQ=255)

 gggTGGGAGGACGGTTTGCACCCTCTTCCCCGAGGGGCGATGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATgg                                                                                                                                     <  2:638157/141‑1 (MQ=255)

 gAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATgg                                                                                                                                     <  2:154642/143‑1 (MQ=255)

      ggAGCACGCTTTCCACCGTCTTCCCAGAGGGGCGAGGGGACTCTCGGAGTATCATGAGGCCGAAAACCCTGTATTTCATGTAATGTATGCCCATAAGCTAAGGTTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATg                                                                                                                                <  2:434045/143‑1 (MQ=255)

       gAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGAGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGc                                                                                                                               <  1:500118/143‑1 (MQ=255)

           aCGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGTCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCCGAGTGTATGGGAATGCTCaa                                                                                                                           >  1:12826/1‑143 (MQ=255)

               ttttCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTg                                                                                                                       >  2:297752/1‑143 (MQ=255)

                 ttCGCCCTCTGCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATGTATTCGCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGa                                                                                                                     <  1:46011/143‑1 (MQ=255)

                  tCACCCGCTGCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGaa                                                                                                                    <  2:634303/143‑1 (MQ=255)

                       ntctTNCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCNCATAAACTCAGATTTANTGCTGCTTCNCGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCgg                                                                                                               <  1:562233/142‑1 (MQ=255)

                       cttttgccAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAATACTCTGCTTTTCAGGTAATTGATGCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCgg                                                                                                               <  2:145628/137‑1 (MQ=255)

                         catgccAGAGGGGCGAGGGGAAGCACGGAGTATCATGAGGCCGAAAACTCTGCGATTCAGGGAATTGATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAg                                                                                                             <  1:4162/139‑1 (MQ=255)

                             ccAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGttt                                                                                                         >  1:182437/1‑143 (MQ=255)

                              ccgagGGGCTAGGGTACTTTCCGATTATCATGTGGCCGAAAACTCTGCTTTTCAGGTAATTTACTCCCATAAACTCAGAGTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGtttt                                                                                                        <  2:216505/141‑1 (MQ=255)

                                 agGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTg                                                                                                     <  1:460035/143‑1 (MQ=255)

                                   gggCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCa                                                                                                   <  2:363064/143‑1 (MQ=255)

                                   gggCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCa                                                                                                   <  2:51262/143‑1 (MQ=255)

                                            gACTCTCCGAGTATCATGAGGCCGAAAACTATGCTTCTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGCATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGCGGTTTTCTGCAGATTCGTCt                                                                                          >  2:613774/1‑143 (MQ=255)

                                              ctctCCGTGTATCATGAGGCCGTAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGc                                                                                        <  2:435883/143‑1 (MQ=255)

                                                 tcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCCACCGGGAAGCAGGAGGTTTTCTGCAGATTCGCCTGCCag                                                                                     >  1:176450/1‑142 (MQ=255)

                                                              aGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTc                                                                        <  2:113969/143‑1 (MQ=255)

                                                                  cGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcaagc                                                                    <  2:171615/143‑1 (MQ=255)

                                                                    aaaaCTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcaagcaa                                                                  <  1:344663/143‑1 (MQ=255)

                                                                          ctGCTTTTCAGGTAATTTATTCCCATACACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAgg                                                            <  1:233198/143‑1 (MQ=255)

                                                                             cTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGCGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGgaga                                                         >  2:608518/1‑143 (MQ=255)

                                                                              tgttCAGGTCATTAATTCCCAGAAACTAAGTTGTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGAt                                                        <  2:227311/141‑1 (MQ=255)

                                                                                ttCAGGGAATTTAGTCCCATACGCTCAGAGTTACTGCTGCGTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCt                                                      <  1:17074/143‑1 (MQ=255)

                                                                                      tAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAGGCAAGCCAGGAGATCTGCatga                                                >  1:265312/1‑143 (MQ=255)

                                                                                      tAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCatga                                                >  1:200877/1‑143 (MQ=255)

                                                                                         tttATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTACCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCAAGATGt                                             >  1:576681/1‑143 (MQ=255)

                                                                                             ttCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGAAGAAGTTATTCAAGCAAGCCAGCAGCTCTGCATGATGTCTGc                                         >  2:428959/1‑143 (MQ=255)

                                                                                                  aTAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCa                                    >  2:41854/1‑143 (MQ=255)

                                                                                                        tCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTa                              >  1:114982/1‑143 (MQ=255)

                                                                                                          aGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACa                            >  1:591565/1‑143 (MQ=255)

                                                                                                             tttACTGCTGCTTCACGCAGGACCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGCAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAACATTACAttt                         >  1:65816/1‑143 (MQ=255)

                                                                                                                    ctgctTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGgcg                  >  2:147060/1‑143 (MQ=255)

                                                                                                                    ctgctTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGgcg                  <  1:297752/143‑1 (MQ=255)

                                                                                                                       ctTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTg               >  1:67279/1‑143 (MQ=255)

                                                                                                                             gCAGGCTCGGGGTGTAGGGGAATGCTCAACCTGGAAGCCGGGGGTGTTCTGCAGACTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTTTGCACCCAAAATTACATTTATCGGCGCTGGTTcga         <  1:135904/143‑1 (MQ=255)

                                                                                                                             gCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGCTCTGCATGCTGTCTGCGCCCCGTATTACATTTATCTGCTCTGGTTaga         >  1:50091/1‑143 (MQ=255)

                                                                                                                                    cTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGCGATCTGCATGATGTCTGCACCCAAAACTACATTTATCGGCGCTGGTCCGACGAtttc  >  1:291452/1‑142 (MQ=255)

CGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTT  >  NC_000913/4341686‑4341960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A