Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F61 I0 R1	36	35.4	1352518	90.1%	1218618	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,341,824	T→G	33.8%	intergenic (‑196/‑87)	melR ← / → melA	DNA‑binding transcriptional dual regulator MelR/alpha‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,341,824	0	T	G	33.8%	37.3 / 24.7	46	intergenic (‑196/‑87)	melR/melA	DNA‑binding transcriptional dual regulator MelR/alpha‑galactosidase
Reads supporting (aligned to +/- strand): ref base T (13/16); new base G (8/7); total (22/24)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

TGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTC  >  NC_000913/4341684‑4341961

tGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACCCAGGAACCGAGTGTa                                                                                                                                         >  1:270293/1‑143 (MQ=255)

   gAGTGGGAGCACGGTTTTCACCCTCTTCCCAGGGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATgg                                                                                                                                      <  2:100324/143‑1 (MQ=255)

   gAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGGAAACTCTGCTTTTCAGGTGATTTGTTCCCATAAATGCAGATTTACTGCTGCTTCACGCAGGATATGAGTTTATgg                                                                                                                                      >  1:525527/1‑143 (MQ=255)

   gAGGGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATgg                                                                                                                                      <  1:224960/143‑1 (MQ=255)

      tGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGaa                                                                                                                                   <  2:199610/143‑1 (MQ=255)

         aaGCACGGTTTTCACCCTCTTCCCAGCGGGCAGAGGGAACTCACCGAGAATCATAAGGCCGAAAACTCTGCTTTTCAGGTAATTTATACCCAGAAACTCAGAGTTACTGCTGCTTCACACAGGCACGGAGTTTAAGAGAAtac                                                                                                                                >  2:171511/2‑141 (MQ=255)

               ggTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAAcc                                                                                                                          >  1:39155/1‑143 (MQ=255)

                ggtttCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTCATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCGGGATCTGAGTGTATGGGAATGCTCAAccc                                                                                                                         >  2:369922/3‑142 (MQ=255)

                          tctTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAACACTCGGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGa                                                                                                               >  1:528218/1‑143 (MQ=255)

                          tctTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGa                                                                                                               <  1:187528/143‑1 (MQ=255)

                              cccAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACGCAAAATCAATTCTGCTTCACGCAGGATCTGAGTATATGGGAAGGCTCAACCTGGAAGCCGGCGGtt                                                                                                           >  2:584439/1‑143 (MQ=255)

                                  gagGGGCGAGGGGACTCTTCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCt                                                                                                       <  1:264258/143‑1 (MQ=255)

                                      ggCGAGGGGTCTTTCCGAGTATCATGAGGCCGAAGACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTAAGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAg                                                                                                   <  1:29284/143‑1 (MQ=255)

                                            ggggCTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGc                                                                                             <  1:407758/143‑1 (MQ=255)

                                             ggACTCTGCGAGTATCATGAGGCCGAAAGCTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATCCTCAACCTGGAAGCCTGAGGTTTTCTGCAGATTCGcc                                                                                            <  2:111858/143‑1 (MQ=255)

                                                 tctcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGcc                                                                                        >  2:427951/1‑143 (MQ=255)

                                                 tctcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGGTTTCTGCAGATTCGACTgac                                                                                        >  2:348191/1‑141 (MQ=255)

                                                 tctcCGAGTATCATGAGGCCGAAAACTCTGCTTTGCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGcc                                                                                        <  2:646056/143‑1 (MQ=255)

                                                   tcCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGCGGTTTTCTGCAGATTCGCCTGCCat                                                                                      >  1:232606/1‑143 (MQ=255)

                                                      gAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACACAGCTTTACTACTGCTTCATGCAGGATCTGAGCGTATGGGAATGCTCAACCTGGAAGCCGGAAGTTTTCTGCAGATTCGCCTGCCatgca                                                                                   >  2:266961/1‑141 (MQ=255)

                                                      gAGTATCATGAGGCCCAAACCTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCACCCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCatgat                                                                                   <  1:481465/143‑1 (MQ=255)

                                                            cATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGtt                                                                             <  1:580657/143‑1 (MQ=255)

                                                                  gCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcat                                                                       >  2:670112/1‑142 (MQ=255)

                                                                   ccGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTcaag                                                                      <  1:626879/143‑1 (MQ=255)

                                                                           tctGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAg                                                              <  2:672480/143‑1 (MQ=255)

                                                                           tctGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAg                                                              >  2:15313/1‑143 (MQ=255)

                                                                           gctGCTTTTCAGGTAAGTTGTGCCCTTGGTCTCAGGTTTACTGCTGCTTCACGCAGGATCTGAGGTTATGGGAATGCTCAACCTGGAGGCCGGAGGTTTTGTGGTGCTAACTGTTTCACGATGAAGTTAGTCAAGCAAGCCAg                                                              <  2:481745/142‑1 (MQ=255)

                                                                                 tttCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATTCGCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGATATTCAAGCAAGCCAGGAGATc                                                        >  2:657954/1‑143 (MQ=255)

                                                                                      ggTAATTTATGCCCATAAACTCAGATTTACTGCTGCTGCACGCAGGATCTTAGGTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCGTGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCat                                                   <  1:435292/143‑1 (MQ=255)

                                                                                       gTAATTGATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCatg                                                  <  1:328786/143‑1 (MQ=255)

                                                                                        tgtcttATTCCCCCGAACTCAGGTTTACTGCTGGTGCGGGGAGGGTCTGAGTTGATGGGAATGCTCAACCGGGACGTCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCatga                                                 <  1:522605/139‑1 (MQ=255)

                                                                                        tAATTTATTCCCATAAACTCAGATTTACGGCTGCTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCatga                                                 <  1:167857/143‑1 (MQ=255)

                                                                                          aTTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGCACGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGCAGTTATTCAAGCAGCCCAGCAGAGCTCCatgatg                                               >  2:665766/1‑143 (MQ=255)

                                                                                           tctATCCCCATAAACTCAGATTTACTGCTCGTTCACGCAGGATCTCAGTCTATGGGAATGCTCAACCTGGAAGCCGTAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGt                                              <  2:660110/141‑1 (MQ=255)

                                                                                             tATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAACCAGGAGATCTGCATGATGTCt                                            >  1:625258/1‑143 (MQ=255)

                                                                                              cttCCTATATTCTCTGATTTACTGCTTCTTCATGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTGCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTg                                           <  1:35312/142‑1 (MQ=255)

                                                                                                          tCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCGAGCCAGGAGATCTGGAAGATGTGTGCACCCACCATTa                               >  1:303235/1‑143 (MQ=255)

                                                                                                          tCAGATGTACTGCTGCTTCACGCAGGATCTTAGTTTATTGGAATGCTCAACCTGGAAGCCGGAGGTTTCCTGCATATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTa                               <  2:525527/143‑1 (MQ=255)

                                                                                                          tCAGATCTACTGCTGCGTCACGCATGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGACGCTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTa                               <  2:315401/143‑1 (MQ=255)

                                                                                                             gATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGACGTCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACAt                            >  1:125176/1‑143 (MQ=255)

                                                                                                                    tgctgcTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCgg                     >  1:545350/1‑143 (MQ=255)

                                                                                                                    tgctgcTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCCGAACCCAAAATTACATTTATCgg                     >  1:632878/1‑143 (MQ=255)

                                                                                                                    tgctgcTTCACGCAGGATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGCGGTTTTCTGCAGATTCGCCTGCCCTGATGAAGTTATTCAAGCAAGCCAGGCGATCTGCATGATGTCTGCACCCCAACTTACATTTATCgg                     >  1:114618/1‑143 (MQ=255)

                                                                                                                               gCAGGATCTGAGTTTATGGGAATGCCCAACCTGGAAGCCGCAGGGTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTcga          <  2:498929/143‑1 (MQ=255)

                                                                                                                                   gATCTGAGTGTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGAt      >  2:217949/1‑143 (MQ=255)

                                                                                                                                       taggTTTAGGGGAATGCTCAACCTGGAAGCCGGAGGTGTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTc  <  2:278153/140‑1 (MQ=255)

TGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAGTTATTCAAGCAAGCCAGGAGATCTGCATGATGTCTGCACCCAAAATTACATTTATCGGCGCTGGTTCGACGATTTTC  >  NC_000913/4341684‑4341961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A