Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F65 I0 R1 39 317.7 11131448 98.2% 10931081 135.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,561,269 G→A 100% S41S (AGC→AGT)  yjiR ← putative DNA‑binding transcriptional regulator/putative aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,561,2690GA100.0% 964.2 / ‑6.7 245S41S (AGC→AGT) yjiRputative DNA‑binding transcriptional regulator/putative aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (102/141);  minor base C (1/0);  total (103/142)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 248 total aligned reads displayed.

TTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTG  >  CP009273/4561130‑4561408
                                                                                                                                           |                                                                                                                                           
tttCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTg                                                                                                                                             <  2:97305/140‑1 (MQ=255)
  tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCCCGGTACt                                                                                                                                           >  2:2235053/1‑140 (MQ=255)
  tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                           <  1:4208622/140‑1 (MQ=255)
     aCCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGAt                                                                                                                                        >  2:1593741/1‑140 (MQ=255)
         ggCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTg                                                                                                                                    >  2:1407044/1‑140 (MQ=255)
               gCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCg                                                                                                                              <  1:4414403/140‑1 (MQ=255)
               gCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCg                                                                                                                              <  1:1941003/140‑1 (MQ=255)
               gCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCg                                                                                                                              <  2:310785/140‑1 (MQ=255)
               gCACCGGACGCCTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCg                                                                                                                              <  2:1685541/140‑1 (MQ=255)
                    ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                         <  1:3314074/140‑1 (MQ=255)
                    ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                         <  1:2994616/140‑1 (MQ=255)
                    ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                         <  2:4199663/140‑1 (MQ=255)
                    ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                         <  2:3899269/140‑1 (MQ=255)
                    ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                         <  1:4463465/140‑1 (MQ=255)
                      aCGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCt                                                                                                                       <  2:3169237/140‑1 (MQ=255)
                          gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                         >  1:5411510/1‑134 (MQ=255)
                          gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                         >  1:3771176/1‑134 (MQ=255)
                          gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                         <  2:1253367/134‑1 (MQ=255)
                          gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                         <  2:3771176/134‑1 (MQ=255)
                          gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACt                                                                                                                   >  1:1454321/1‑140 (MQ=255)
                          gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACt                                                                                                                   >  1:36926/1‑140 (MQ=255)
                          gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACt                                                                                                                   >  1:3383264/1‑140 (MQ=255)
                          gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGACTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACt                                                                                                                   >  1:1730021/1‑140 (MQ=255)
                               cggcggTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGc                                                                                                              <  2:3600992/140‑1 (MQ=255)
                                    gTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTgcgc                                                                                                         >  2:110404/1‑140 (MQ=255)
                                    gTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTgcgc                                                                                                         >  1:1119972/1‑140 (MQ=255)
                                    gTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTgcgc                                                                                                         >  2:2951976/1‑140 (MQ=255)
                                        tGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCg                                                                                                     <  2:2637798/140‑1 (MQ=255)
                                        tGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCg                                                                                                     <  2:2143767/140‑1 (MQ=255)
                                            ggcTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACgg                                                                                                 >  1:4568575/1‑140 (MQ=255)
                                            ggcTGGGCGTTACGTTGTGCGACAAAATAACCCGAACGCGGCGGCGGAGTGATGAGCTTCATCGTCTCCAGCGGCTGATACGCGTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTGAAGCTGCGCACCGACgg                                                                                                 >  2:4839340/1‑140 (MQ=255)
                                              cTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCa                                                                                               >  1:3511853/1‑140 (MQ=255)
                                              cTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCa                                                                                               >  1:225071/1‑140 (MQ=255)
                                               tGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCaa                                                                                              >  1:1090569/1‑140 (MQ=255)
                                                 ggCTTTACGTAGTGCGACAAAATACCCCGAACGCGGCTGCGGTGGGCTGAGCGTCATCGTCTCCCGCGTCTGATACGCCTGAGGCACGGTCCTGGACCGGACGCCGTGCTCCTGACTTAAGCGGCGCCCCGAGGGCCAtt                                                                                            >  2:4603594/1‑140 (MQ=255)
                                                  gCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAAttt                                                                                           <  2:446282/140‑1 (MQ=255)
                                                   cTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTtc                                                                                          <  1:1831891/140‑1 (MQ=255)
                                                       aCGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTccc                                                                                      <  1:2274256/140‑1 (MQ=255)
                                                       aCGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTccc                                                                                      <  1:2494841/140‑1 (MQ=255)
                                                           tgtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTg                                                                                  <  2:1876004/140‑1 (MQ=255)
                                                           tgtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTg                                                                                  >  2:2499941/1‑140 (MQ=255)
                                                           tgtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTg                                                                                  <  2:3489291/140‑1 (MQ=255)
                                                            gtgCGACAAAATAACCCGCACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCGAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTGAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                                 <  2:3978001/140‑1 (MQ=255)
                                                            gtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                                 <  1:3614393/140‑1 (MQ=255)
                                                            gtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                                 <  1:644077/140‑1 (MQ=255)
                                                             tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                                >  1:116910/1‑140 (MQ=255)
                                                             tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                                >  2:432905/1‑140 (MQ=255)
                                                             tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                                >  1:3260697/1‑140 (MQ=255)
                                                             tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                                >  1:2994433/1‑140 (MQ=255)
                                                             tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                                >  2:672334/1‑140 (MQ=255)
                                                             tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                                >  1:784387/1‑140 (MQ=255)
                                                             tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGGGCTCCTGACTTAAGCTGCGCACCGCCGGCAATTTCTCCCCGTGAc                                                                                >  1:3913696/1‑140 (MQ=255)
                                                                gACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGAt                                                                             >  1:2232473/1‑140 (MQ=255)
                                                                 aCAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATa                                                                            <  1:5526665/140‑1 (MQ=255)
                                                                 aCAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATa                                                                            <  2:358912/140‑1 (MQ=255)
                                                                  cAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                           >  2:5352053/1‑140 (MQ=255)
                                                                      agaaCCCGTACGCGGCTGCGGAGTGATGAGCGTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGcc                                                                       <  2:3094740/138‑1 (MQ=255)
                                                                      aTAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGcc                                                                       <  2:1283009/140‑1 (MQ=255)
                                                                      aTAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGcc                                                                       <  2:2749869/140‑1 (MQ=255)
                                                                      aTAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGcc                                                                       <  1:4899621/140‑1 (MQ=255)
                                                                                     cTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGtt                                                        >  2:32845/1‑140 (MQ=255)
                                                                                        cGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCgg                                                     >  1:4680751/1‑140 (MQ=255)
                                                                                             tgatgaGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                                 <  1:220827/107‑1 (MQ=255)
                                                                                                tgaGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCaga                                             <  2:4252218/140‑1 (MQ=255)
                                                                                                 gaGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCagag                                            <  2:883728/140‑1 (MQ=255)
                                                                                                    cTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCg                                         >  1:837740/1‑140 (MQ=255)
                                                                                                     ttCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGt                                                                                   >  2:4360555/1‑97 (MQ=255)
                                                                                                       cATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCa                                      <  1:3175863/140‑1 (MQ=255)
                                                                                                         tCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCGGTTCGGCAAGCAGAGTCGCCAGa                                    >  2:3370307/1‑140 (MQ=255)
                                                                                                         tCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGa                                    <  1:3109764/140‑1 (MQ=255)
                                                                                                          cGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGAt                                   <  1:1933177/140‑1 (MQ=255)
                                                                                                            tctcCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGt                                 >  1:1509763/1‑140 (MQ=255)
                                                                                                            tctcCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGt                                 >  1:5221363/1‑140 (MQ=255)
                                                                                                             ctcCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGtt                                >  2:3235746/1‑140 (MQ=255)
                                                                                                              tcCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTg                               >  2:2870224/1‑140 (MQ=255)
                                                                                                              tcCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTg                               >  1:1319517/1‑140 (MQ=255)
                                                                                                              tcCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTg                               >  2:1430532/1‑140 (MQ=255)
                                                                                                                      cTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAAcgcg                       >  1:2820197/1‑140 (MQ=255)
                                                                                                                      cTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAAcgcg                       <  2:485572/140‑1 (MQ=255)
                                                                                                                      cTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAAcgcg                       >  1:928481/1‑140 (MQ=255)
                                                                                                                      cTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAAcgcg                       >  1:163765/1‑140 (MQ=255)
                                                                                                                        gATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTc                     <  2:4767062/140‑1 (MQ=255)
                                                                                                                           aagCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCGTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATg                  <  1:5184352/138‑1 (MQ=255)
                                                                                                                           aCGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCACTCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATg                  <  1:4738567/140‑1 (MQ=255)
                                                                                                                           aCGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATg                  <  1:1589866/140‑1 (MQ=255)
                                                                                                                            cGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGc                 <  1:4434483/140‑1 (MQ=255)
                                                                                                                            cGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGc                 <  1:2898042/140‑1 (MQ=255)
                                                                                                                              ccTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTg               <  1:1338950/140‑1 (MQ=255)
                                                                                                                              ccTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTg               <  1:3858918/140‑1 (MQ=255)
                                                                                                                                tgctgcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTa             <  2:1973371/140‑1 (MQ=255)
                                                                                                                                tgctgcACGGTACTGAGACTGCCGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTa             <  2:2562526/140‑1 (MQ=255)
                                                                                                                                 gctgcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTAt            <  1:5047214/140‑1 (MQ=255)
                                                                                                                                 gctgcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTAt            >  2:5052711/1‑140 (MQ=255)
                                                                                                                                  ctgcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                                 >  2:611416/1‑70 (MQ=255)
                                                                                                                                    gcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTcc         <  2:5395644/140‑1 (MQ=255)
                                                                                                                                    gcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTcc         <  1:2403543/140‑1 (MQ=255)
                                                                                                                                      aCGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCtt       <  1:3559141/140‑1 (MQ=255)
                                                                                                                                        ggTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAt     <  2:4079409/140‑1 (MQ=255)
                                                                                                                                        ggTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAt     <  1:4288977/140‑1 (MQ=255)
                                                                                                                                           gCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAtgtg  >  1:4002192/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTATGTG  >  CP009273/4561130‑4561408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A