Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F63 I0 R1 89 546.6 19089912 98.5% 18803563 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,561,269 G→A 100% S41S (AGC→AGT)  yjiR ← putative DNA‑binding transcriptional regulator/putative aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,561,2690GA98.1% 2036.8 / 14.6 528S41S (AGC→AGT) yjiRputative DNA‑binding transcriptional regulator/putative aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (6/4);  new base A (216/300);  total (223/305)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 533 total aligned reads displayed.

TCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAT  >  CP009273/4561132‑4561405
                                                                                                                                         |                                                                                                                                        
tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        >  1:4508261/1‑140 (MQ=255)
tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        <  2:6447969/140‑1 (MQ=255)
tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        >  1:4963016/1‑140 (MQ=255)
tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        >  1:5935626/1‑140 (MQ=255)
tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        <  2:4269711/140‑1 (MQ=255)
tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        <  2:4053101/140‑1 (MQ=255)
tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        <  1:9127146/140‑1 (MQ=255)
tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        <  2:1479418/140‑1 (MQ=255)
   aCCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGAt                                                                                                                                     >  1:1047004/1‑140 (MQ=255)
   aCCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGAt                                                                                                                                     >  1:9532965/1‑140 (MQ=255)
   aCCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGAt                                                                                                                                     >  2:2177386/1‑140 (MQ=255)
          gcTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACg                                                                                                                              <  2:110889/140‑1 (MQ=255)
            tGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGcc                                                                                                                            <  1:3245799/140‑1 (MQ=255)
            tGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGcc                                                                                                                            <  2:4721818/140‑1 (MQ=255)
                  ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                      <  1:2706210/140‑1 (MQ=255)
                  ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                      <  1:2437291/140‑1 (MQ=255)
                  ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                      <  1:935848/140‑1 (MQ=255)
                  ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                      <  1:3945432/140‑1 (MQ=255)
                  ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                      <  1:4381170/140‑1 (MQ=255)
                   gACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTcc                                                                                                                     >  2:4923628/1‑140 (MQ=255)
                       cgTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACACAAGAACCGGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGAGACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGAc                                                                                                                 <  1:1155602/140‑1 (MQ=255)
                        gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACt                                                                                                                >  2:4140894/1‑140 (MQ=255)
                        gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACt                                                                                                                >  2:941830/1‑140 (MQ=255)
                        gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACt                                                                                                                >  1:2255423/1‑140 (MQ=255)
                            tCGGCGGTACTTGCGGCTGGGCTTTACGTTGTGCGACAATAGAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGCCGCCGTGCTCCTGACTTAAg                                                                                                            <  1:1628082/140‑1 (MQ=255)
                             cggcggTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGc                                                                                                           >  2:5603824/1‑140 (MQ=255)
                             cggcggTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGc                                                                                                           >  1:7338318/1‑140 (MQ=255)
                                        gcggcTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCt                                                                                                                    >  1:5221296/1‑120 (MQ=255)
                                        gcggcTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGAc                                                                                                >  2:2294996/1‑140 (MQ=255)
                                        gcggcTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGAc                                                                                                >  2:7845040/1‑140 (MQ=255)
                                          ggcTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACgg                                                                                              <  1:3651592/140‑1 (MQ=255)
                                            cTGGGCTTTACGTTGTGCGACACAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCa                                                                                            <  2:6297394/140‑1 (MQ=255)
                                            cTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCa                                                                                            <  2:5147858/140‑1 (MQ=255)
                                             tGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCg                                                                                                                           >  2:1842025/1‑108 (MQ=255)
                                             tGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCaa                                                                                           >  1:8765264/1‑140 (MQ=255)
                                             tGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCaa                                                                                           >  1:2598864/1‑140 (MQ=255)
                                             tGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCaa                                                                                           >  1:645274/1‑140 (MQ=255)
                                             tGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCaa                                                                                           >  1:2103071/1‑140 (MQ=255)
                                             tGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCaa                                                                                           >  2:7137990/1‑140 (MQ=255)
                                               ggCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAAtt                                                                                         >  2:2622006/1‑140 (MQ=255)
                                                gCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAAttt                                                                                        <  2:8167168/140‑1 (MQ=255)
                                                     aCGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTccc                                                                                   <  2:3177005/140‑1 (MQ=255)
                                                     aCGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTccc                                                                                   <  2:3872272/140‑1 (MQ=255)
                                                     aCGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTccc                                                                                   <  2:1282882/140‑1 (MQ=255)
                                                         tgtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTg                                                                               >  2:340764/1‑140 (MQ=255)
                                                          gtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                              <  2:6822711/140‑1 (MQ=255)
                                                          gtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                              >  1:5118171/1‑140 (MQ=255)
                                                          gtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                              >  1:7745882/1‑140 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCACTTTCTCCCCGTGAc                                                                             >  2:758903/1‑140 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                              >  2:9102758/1‑139 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  1:5449606/1‑140 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  1:9040003/1‑140 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  1:6200377/1‑140 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  1:3486419/1‑140 (MQ=255)
                                                               aCAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATa                                                                         <  1:7497355/140‑1 (MQ=255)
                                                                cAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        <  1:7845040/140‑1 (MQ=255)
                                                                cAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        <  2:8957942/140‑1 (MQ=255)
                                                                cAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        >  2:2882671/1‑140 (MQ=255)
                                                                cAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        <  1:6777333/140‑1 (MQ=255)
                                                                cAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        <  1:4798158/140‑1 (MQ=255)
                                                                cAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        <  1:8521912/140‑1 (MQ=255)
                                                                cAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        <  1:5603824/140‑1 (MQ=255)
                                                                cAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        <  2:6109008/140‑1 (MQ=255)
                                                                cAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        <  2:7416764/140‑1 (MQ=255)
                                                                    aTAACCCGCCCGCGGCGGCGGAGTGATGAGCGTCATCGTCGCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAAGTTCTCCCCGTGACGATACAGcc                                                                    <  2:1684144/140‑1 (MQ=255)
                                                                    aTAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGcc                                                                    <  2:8046429/140‑1 (MQ=255)
                                                                    aTAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGcc                                                                    <  1:1375038/140‑1 (MQ=255)
                                                                     ccaCCCGAACCCGCCAGCCCACTGAGGAGCCTCATCGTCTCCAGCGTCTGATACGCCACCTCCACGGTCCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGccc                                                                   <  1:5528314/138‑1 (MQ=255)
                                                                           gAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTc                                                             <  2:7279579/140‑1 (MQ=255)
                                                                            aaCGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCa                                                            <  2:3526422/140‑1 (MQ=255)
                                                                            aaCGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCa                                                            <  2:5271237/140‑1 (MQ=255)
                                                                             aCGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCaa                                                           >  1:8598882/1‑140 (MQ=255)
                                                                               gcgGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATc                                                         >  1:7221253/1‑140 (MQ=255)
                                                                                   cTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGtt                                                     >  1:1840527/1‑140 (MQ=255)
                                                                                   cTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGtt                                                     >  1:5737042/1‑140 (MQ=255)
                                                                                             atgaGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAAAGCCCTTGCTCAATCCGTTCGGCAAGCag                                           >  1:1946443/1‑140 (MQ=255)
                                                                                              ttaGCTTCATCGTCTCCAGCGTCTGATACGCCTTCTGCACGGTACTGATACTGACGCCGTGCTCCTGACGTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCaga                                          <  2:372430/138‑1 (MQ=255)
                                                                                              tgaGATTCATCGTCTACAGCGTCTGATACGCCAGCTGCACTGTAGTGATACTGACGCCTTTCTCCTGAATTAAGCTGCGCACCGACGGAAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCaga                                          <  1:6115137/140‑1 (MQ=255)
                                                                                                     cATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTccc                                                                                   >  2:8596632/1‑92 (MQ=255)
                                                                                                      aTCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAg                                  <  2:6528550/140‑1 (MQ=255)
                                                                                                         gTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCGCCTGAATTAAGCTGCGCACCGACGGCAATTTCTCCCCGGGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATg                               <  1:3025712/140‑1 (MQ=255)
                                                                                                           ctcCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGtt                             <  1:5855099/140‑1 (MQ=255)
                                                                                                             ccAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGa                           <  1:405822/140‑1 (MQ=255)
                                                                                                               aGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATa                         <  2:5883524/140‑1 (MQ=255)
                                                                                                               aGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATa                         <  2:4824898/140‑1 (MQ=255)
                                                                                                                 cGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAAc                       >  2:4527646/1‑140 (MQ=255)
                                                                                                                 cGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAAc                       >  2:8825777/1‑140 (MQ=255)
                                                                                                                 cGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAAc                       >  2:8338578/1‑140 (MQ=255)
                                                                                                                      gATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTc                  <  1:6975706/140‑1 (MQ=255)
                                                                                                                       aTACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGGTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCa                 <  1:2193486/140‑1 (MQ=255)
                                                                                                                            ccTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTg            <  2:6701467/140‑1 (MQ=255)
                                                                                                                             ctgctgCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGt           >  2:1993599/1‑140 (MQ=255)
                                                                                                                                 tgcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTc       <  2:6990046/140‑1 (MQ=255)
                                                                                                                                 tgcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTc       <  2:9241539/140‑1 (MQ=255)
                                                                                                                                 tgcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTc       <  2:77300/140‑1 (MQ=255)
                                                                                                                                  gcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCTTCATGCTGTATTcc      >  2:303123/1‑140 (MQ=255)
                                                                                                                                     cGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTa   >  1:6555818/1‑140 (MQ=255)
                                                                                                                                      ggTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAt  <  2:5935626/140‑1 (MQ=255)
                                                                                                                                      ggTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAt  <  1:3827386/140‑1 (MQ=255)
                                                                                                                                      ggTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCAGTGACGATACAGCCCGTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAt  <  1:818846/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAT  >  CP009273/4561132‑4561405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A