Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F61 I0 R1	274	47.1	1889746	95.1%	1797148	135.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,561,269	G→A	80.9%	S41S (AGC→AGT)	yjiR ←	putative DNA‑binding transcriptional regulator/putative aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,561,269	0	G	A	80.9%	160.3 / 32.8	68	S41S (AGC→AGT)	yjiR	putative DNA‑binding transcriptional regulator/putative aminotransferase
Reads supporting (aligned to +/- strand): ref base G (5/8); new base A (25/30); total (30/38)
Fisher's exact test for biased strand distribution p-value = 7.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAT  >  CP009273/4561132‑4561405

tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        <  2:864615/140‑1 (MQ=255)

   aCCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGAt                                                                                                                                     >  1:899743/1‑140 (MQ=255)

   aCCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGAt                                                                                                                                     >  1:230630/1‑140 (MQ=255)

   aCCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGAt                                                                                                                                     >  1:24286/1‑140 (MQ=255)

                  ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                      <  1:9239/140‑1 (MQ=255)

                  ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                      <  1:239733/140‑1 (MQ=255)

                        gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACt                                                                                                                >  1:402595/1‑140 (MQ=255)

                        gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACt                                                                                                                >  1:566233/1‑140 (MQ=255)

                        gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACt                                                                                                                >  1:62987/1‑140 (MQ=255)

                        gTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGCCTGCGGAGTGATGAGATTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACt                                                                                                                >  2:599716/1‑140 (MQ=255)

                                  gTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTgcgc                                                                                                      >  2:479358/1‑140 (MQ=255)

                                        gcggcTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGAc                                                                                                <  2:230630/140‑1 (MQ=255)

                                        gcggcTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGAc                                                                                                <  2:899743/140‑1 (MQ=255)

                                        gcggcTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGAc                                                                                                <  2:24286/140‑1 (MQ=255)

                                        gcggcTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTCCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGAc                                                                                                >  2:511896/1‑140 (MQ=255)

                                             tGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCaa                                                                                           >  2:514555/1‑140 (MQ=255)

                                             tGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCaa                                                                                           >  1:12247/1‑140 (MQ=255)

                                                   ttACGTTGTGCGACAAAAGAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTtctc                                                                                     <  2:638590/140‑1 (MQ=255)

                                                    tACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTcc                                                                                    >  2:525295/1‑140 (MQ=255)

                                                    tACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTcc                                                                                    >  2:696465/1‑140 (MQ=255)

                                                    tACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTcc                                                                                    <  2:566233/140‑1 (MQ=255)

                                                    tACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTcc                                                                                    >  2:591826/1‑140 (MQ=255)

                                                      cGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTcccc                                                                                  >  1:9911/1‑140 (MQ=255)

                                                      cGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTcccc                                                                                  >  1:892830/1‑140 (MQ=255)

                                                      cGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTcccc                                                                                  >  1:207246/1‑140 (MQ=255)

                                                      cGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTcccc                                                                                  >  2:553722/1‑140 (MQ=255)

                                                      cGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTcccc                                                                                  >  2:671514/1‑140 (MQ=255)

                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  2:303955/1‑140 (MQ=255)

                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  2:934397/1‑140 (MQ=255)

                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        <  2:724435/81‑1 (MQ=255)

                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        <  2:282181/81‑1 (MQ=255)

                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        >  1:282181/1‑81 (MQ=255)

                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        >  1:724435/1‑81 (MQ=255)

                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCt                                                                                                                    >  1:384867/1‑101 (MQ=255)

                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCt                                                                                                                    >  1:910221/1‑101 (MQ=255)

                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCt                                                                                                                    <  2:910221/101‑1 (MQ=255)

                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCt                                                                                                                    <  2:384867/101‑1 (MQ=255)

                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  1:120220/1‑140 (MQ=255)

                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  1:804677/1‑140 (MQ=255)

                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  1:33833/1‑140 (MQ=255)

                                                                    aTAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGcc                                                                    <  1:640598/140‑1 (MQ=255)

                                                                    aTAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGcc                                                                    <  1:520126/140‑1 (MQ=255)

                                                                    aTAACCAGAACGCGGCTGCGGAGTGATGAGCGTCATCGTCTACAGCGTCTGATACGCCTGCTTCACGGTACTGATACTGCCGCCGTTCTCCTGACTTAATCTGCGCACCGACGGAAATTTCTGCCCGTGACGATACAGcc                                                                    <  1:184530/140‑1 (MQ=255)

                                                                     tAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGccc                                                                   <  2:25813/140‑1 (MQ=255)

                                                                     tAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTTACTTAATCTGCGGTCTTAATTAAATTTCTCCCGGTGACGAttaatgtc                                                                   <  1:591826/140‑8 (MQ=255)

                                                                     tAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAATCTGCTCAAATAATTAAATTTCTCCCCGTGACGAttaatgtc                                                                   <  1:525295/140‑8 (MQ=255)

                                                                      aaCCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCt                                                                  <  2:402595/140‑1 (MQ=255)

                                                                                                  cTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCg                                      <  1:397128/140‑1 (MQ=255)

                                                                                                   ttCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGc                                     <  1:553722/140‑1 (MQ=255)

                                                                                                   ttCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGc                                     <  1:671514/140‑1 (MQ=255)

                                                                                                     cATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCa                                   >  1:53902/1‑140 (MQ=255)

                                                                                                               aGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATa                         <  2:864170/140‑1 (MQ=255)

                                                                                                               aGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATa                         <  2:82529/140‑1 (MQ=255)

                                                                                                               aGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCGTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATa                         <  2:354149/140‑1 (MQ=255)

                                                                                                               aGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATGTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATa                         <  2:764075/140‑1 (MQ=255)

                                                                                                                      gATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCAGCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTc                  <  1:791060/140‑1 (MQ=255)

                                                                                                                      gATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTc                  <  1:236057/140‑1 (MQ=255)

                                                                                                                      gATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTc                  <  1:90866/140‑1 (MQ=255)

                                                                                                                      gATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTc                  <  1:604612/140‑1 (MQ=255)

                                                                                                                       aTACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCa                 <  2:892830/140‑1 (MQ=255)

                                                                                                                       aTACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCa                 <  2:9911/140‑1 (MQ=255)

                                                                                                                       aTACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCa                 <  2:207246/140‑1 (MQ=255)

                                                                                                                       aTACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCa                 <  1:941624/140‑1 (MQ=255)

                                                                                                                       aTACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCa                 <  1:631762/140‑1 (MQ=255)

                                                                                                                             ctgctgCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGt           >  2:294711/1‑140 (MQ=255)

                                                                                                                             ctgctgCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGt           <  1:514555/140‑1 (MQ=255)

                                                                                                                                      ggTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAt  <  2:12247/140‑1 (MQ=255)

                                                                                                                                      ggTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAt  <  1:490360/140‑1 (MQ=255)

TCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAT  >  CP009273/4561132‑4561405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A