Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F65 I0 R1 70 466.8 16344998 98.3% 16067133 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,561,269 G→A 100% S41S (AGC→AGT)  yjiR ← putative DNA‑binding transcriptional regulator/putative aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,561,2690GA99.1% 1819.8 / 0.8 459S41S (AGC→AGT) yjiRputative DNA‑binding transcriptional regulator/putative aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base A (188/267);  total (191/268)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 459 total aligned reads displayed.

TCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAT  >  CP009273/4561132‑4561405
                                                                                                                                         |                                                                                                                                        
tCCACCGGGCGCTGCCCCGGCCGCGACCTCGGCGGGACTGGCGGCGGGGCTGTACGTCGTGCGACAAACTAACCCGAACGCGGCTGCTGAGTGATGAGCTTCATCGTCTCCAGCGTATGATACGCCTGCTGCACGGTACt                                                                                                                                        <  2:2492092/140‑1 (MQ=255)
tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        <  1:1155414/140‑1 (MQ=255)
tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        <  1:1707967/140‑1 (MQ=255)
tCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        <  1:5178357/140‑1 (MQ=255)
  cACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGa                                                                                                                                      <  2:1937096/140‑1 (MQ=255)
  cACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGa                                                                                                                                      >  2:4196279/1‑140 (MQ=255)
   aCCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGAt                                                                                                                                     >  2:1610504/1‑140 (MQ=255)
   aCCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGAt                                                                                                                                     >  2:1361776/1‑140 (MQ=255)
     cGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATAc                                                                                                                                   <  2:5671616/140‑1 (MQ=255)
     cGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATAc                                                                                                                                   <  1:855587/140‑1 (MQ=255)
       ggCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTg                                                                                                                                 >  2:2069219/1‑140 (MQ=255)
       ggCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTg                                                                                                                                 >  2:5906092/1‑140 (MQ=255)
          gcTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACg                                                                                                                              <  1:22695/140‑1 (MQ=255)
             gCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCg                                                                                                                           <  1:3263244/140‑1 (MQ=255)
                  ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                      <  2:7213920/140‑1 (MQ=255)
                  ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                      <  2:5579980/140‑1 (MQ=255)
                  ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                      <  1:6250521/140‑1 (MQ=255)
                  ggACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                      <  1:1449774/140‑1 (MQ=255)
                  ggACGAGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTTATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                      <  2:5359525/140‑1 (MQ=255)
                   gACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACt                                                                                                                                        >  2:683194/1‑121 (MQ=255)
                   gACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTcc                                                                                                                     >  1:7427194/1‑140 (MQ=255)
                   gACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTcc                                                                                                                     >  1:2847977/1‑140 (MQ=255)
                    aCGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCt                                                                                                                    <  2:3587276/140‑1 (MQ=255)
                         taaTCGGCCGTACTGGCGGCTGGGCTTTACGTTGAGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACtt                                                                                                               <  2:6484267/138‑1 (MQ=255)
                             cggcggTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAAAGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGc                                                                                                           >  1:2130338/1‑140 (MQ=255)
                                  gTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTgcgc                                                                                                      >  2:3877719/1‑140 (MQ=255)
                                  gTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTgcgc                                                                                                      >  1:1824021/1‑140 (MQ=255)
                                     cTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCAcc                                                                                                   <  2:5209408/140‑1 (MQ=255)
                                             tGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCaa                                                                                           >  2:2266617/1‑140 (MQ=255)
                                             tGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCaa                                                                                           >  2:7108067/1‑140 (MQ=255)
                                             tGGGCTTTACGTTGTGCGAAAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCATCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCATGACTTAAGCTGCGCACCGACGGCaa                                                                                           >  1:3675615/1‑140 (MQ=255)
                                                     aCGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTccc                                                                                   <  1:758228/140‑1 (MQ=255)
                                                      cGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTcccc                                                                                  >  1:5023067/1‑140 (MQ=255)
                                                      cGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTcccc                                                                                  >  1:5416925/1‑140 (MQ=255)
                                                          gtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGTGCACCGACGGCAATTTCTCCCCGTGa                                                                              <  2:6536997/140‑1 (MQ=255)
                                                          gtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                              <  1:7757413/140‑1 (MQ=255)
                                                          gtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                              <  1:6724834/140‑1 (MQ=255)
                                                          gtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                              <  1:3525967/140‑1 (MQ=255)
                                                          gtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                              <  2:2847977/140‑1 (MQ=255)
                                                          gtgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGAAATTTCTCCCCGTGa                                                                              <  2:4748070/140‑1 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCg                                                                                                                           <  2:7396722/94‑1 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTc                                                                                                                      <  1:6472957/99‑1 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACgg                                                                                              <  1:8067986/123‑1 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  1:6898492/1‑140 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  2:6409279/1‑140 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  1:1521934/1‑140 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  2:4497263/1‑140 (MQ=255)
                                                           tgCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                             >  2:675654/1‑140 (MQ=255)
                                                               aCAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATa                                                                         <  2:6279897/140‑1 (MQ=255)
                                                               aCAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATa                                                                         <  1:2763512/140‑1 (MQ=255)
                                                               aCAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATa                                                                         <  1:5610222/140‑1 (MQ=255)
                                                                cAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        <  2:1091231/140‑1 (MQ=255)
                                                                cAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        <  2:3374182/140‑1 (MQ=255)
                                                                cAAAAGAACCCGAACGCGGCTGCGGAGTGATGCGCTTCATCGTCGCCAGCGTCGGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAAGTTCTCCCCGTGACGATag                                                                        <  2:7292394/140‑2 (MQ=255)
                                                                    atcaCCCGACCGCGGCTGCGGAGTGCAGAGCTTCCTCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGAGACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGcc                                                                    <  2:3302645/137‑1 (MQ=255)
                                                                    aTAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGcc                                                                    <  2:7509220/140‑1 (MQ=255)
                                                                    aTAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGcc                                                                    <  1:593095/140‑1 (MQ=255)
                                                                     tAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGccc                                                                   >  2:827566/1‑140 (MQ=255)
                                                                     tAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGccc                                                                   >  2:7832835/1‑140 (MQ=255)
                                                                     tAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGccc                                                                   >  2:2598978/1‑140 (MQ=255)
                                                                              cgcgGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAAt                                                          <  1:7657753/140‑1 (MQ=255)
                                                                                   cTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGtt                                                     >  2:1663775/1‑140 (MQ=255)
                                                                                     gCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCg                                                   <  1:2059712/140‑1 (MQ=255)
                                                                                         aGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        <  1:1264287/115‑1 (MQ=255)
                                                                                         aGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        <  1:2553111/115‑1 (MQ=255)
                                                                                         aGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCaa                                               <  1:242939/140‑1 (MQ=255)
                                                                                         aGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCaa                                               <  1:7385358/140‑1 (MQ=255)
                                                                                           tgatgaGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATAc                                                                        <  1:5732513/113‑1 (MQ=255)
                                                                                           tgatgaGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGc                                             <  1:3810254/140‑1 (MQ=255)
                                                                                           tgatgaGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGc                                             >  2:4052612/1‑140 (MQ=255)
                                                                                           tgatgaGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGc                                             >  2:4326549/1‑140 (MQ=255)
                                                                                            gatgaGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCa                                            <  2:3675615/140‑1 (MQ=255)
                                                                                            gatgaGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCa                                            <  1:4768502/140‑1 (MQ=255)
                                                                                                aGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGt                                        >  2:3220294/1‑140 (MQ=255)
                                                                                                     cATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCa                                   >  2:2976810/1‑140 (MQ=255)
                                                                                                     cATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCa                                   >  2:1781923/1‑140 (MQ=255)
                                                                                                            tcCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTg                            >  1:6057309/1‑140 (MQ=255)
                                                                                                            tcCAGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTg                            >  1:4363198/1‑140 (MQ=255)
                                                                                                               aGCGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATa                         <  1:7210982/140‑1 (MQ=255)
                                                                                                                 cGTCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTccc                                                                                   >  1:949545/1‑80 (MQ=255)
                                                                                                                   tCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAATCAGAGTCGCCAGATGTTGCTAActc                     >  1:4098983/1‑138 (MQ=255)
                                                                                                                   tCTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAAcgc                     >  1:4208761/1‑140 (MQ=255)
                                                                                                                    cTGATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAAcgcg                    <  2:7957222/140‑1 (MQ=255)
                                                                                                                      gATACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTc                  <  1:1044078/140‑1 (MQ=255)
                                                                                                                       aTACGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCa                 <  1:3159554/140‑1 (MQ=255)
                                                                                                                         aCGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATg               <  2:6062832/140‑1 (MQ=255)
                                                                                                                         aCGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATg               <  1:4637984/140‑1 (MQ=255)
                                                                                                                         aCGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATg               <  1:6831522/140‑1 (MQ=255)
                                                                                                                         aCGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATg               <  1:4015016/140‑1 (MQ=255)
                                                                                                                         aCGCCTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAAGTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATg               <  1:7108067/140‑1 (MQ=255)
                                                                                                                            ccTGCTGCACGGTACTGATACTGCCGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTg            <  2:4438404/140‑1 (MQ=255)
                                                                                                                            ccTGCTGCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTg            <  2:3131501/140‑1 (MQ=255)
                                                                                                                             ctgctgCACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGt           >  2:7709654/1‑140 (MQ=255)
                                                                                                                               gctgcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTAt         <  2:3972852/140‑1 (MQ=255)
                                                                                                                                 tgcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTc       <  2:1492529/140‑1 (MQ=255)
                                                                                                                                 tgcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTc       <  1:4463275/140‑1 (MQ=255)
                                                                                                                                 tgcACGGTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTc       >  1:7956514/1‑140 (MQ=255)
                                                                                                                                      ggTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAt  >  2:5851863/1‑140 (MQ=255)
                                                                                                                                      ggTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAt  <  2:2154872/140‑1 (MQ=255)
                                                                                                                                      ggTACTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAt  <  1:7291631/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCTGTATTCCTTAT  >  CP009273/4561132‑4561405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A