Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F18 I1 R1	18	29.5	1031908	94.0%	969993	141.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,110,747	G→T	intergenic (+62/+784)	cdh → / ← yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,110,747	0	G	T	100.0%	106.1 / NA	36	intergenic (+62/+784)	cdh/yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (22/14); total (22/14)

ACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCC  >  NC_000913_3_pae_tpiA/4110613‑4110888

aCGAAATCTACTGACGCTTCACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGc                                                                                                                                 >  1:75886/1‑149 (MQ=255)

        tACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCGGACTTAAGGCTGCCCCGCCGCGCGCGGTTTGGc                                                                                                                         >  2:198114/1‑149 (MQ=255)

          cTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCa                                                                                                                       >  1:63425/1‑149 (MQ=255)

           tGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCaa                                                                                                                      >  1:56887/1‑149 (MQ=255)

                cTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCactcata                                                                                                                 >  2:128828/1‑143 (MQ=255)

                  tAACCGTGCTTTAACCTAAGAAATTCAGTATCATCAGTGAAAAATTCTGGGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAg                                                                                                               <  1:299364/149‑1 (MQ=255)

                  tAACCGTGATGCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGGTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAg                                                                                                               <  1:110957/149‑1 (MQ=255)

                                   aGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTc                                                                                              >  2:334997/1‑149 (MQ=255)

                                      aaaTTCAGGATCATGAGAGAGAGAGTTTGCGTTAAGGCGTAGAGTGAAGGAAAGGAAGTGCCGGATATGAAATCCGGCACCTGGCAGACTTAAGGCTGCCCCGGCGCGAGCGGTTTCGCCAATTCTAAGATCTGAAGATATGGGTCtgt                                                                                           <  2:106657/149‑3 (MQ=255)

                                               atcttcAGGGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGCTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTccc                                                                                  >  2:396021/1‑147 (MQ=255)

                                                      gtgtGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgcg                                                                           >  2:512012/1‑149 (MQ=255)

                                                         tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                        >  1:259513/1‑149 (MQ=255)

                                                         tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                        >  1:444332/1‑149 (MQ=255)

                                                         tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                        >  2:57540/1‑149 (MQ=255)

                                                             aTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTGTTGCCTTCACGGCTGCGCTTGCCa                                                                    <  1:445678/149‑1 (MQ=255)

                                                                 tGCATTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGGGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCaa                                                                <  1:173778/149‑1 (MQ=255)

                                                                   cGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAaca                                                              <  1:292570/149‑1 (MQ=255)

                                                                     ttAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGACCTTAAGATATGGGTCTTTTGCCTTCACGGATGCGCTTGCCAGACACACt                                                            >  2:332072/1‑149 (MQ=255)

                                                                        aGGCGAAGAGTTAAGGAAAGTAAGTGCTGGATATGTAATCCGGCACTTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAAATCTAAGACCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTccc                                                         <  1:68111/149‑1 (MQ=255)

                                                                              agagTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAAATTTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTg                                                   <  1:203920/149‑1 (MQ=255)

                                                                                  ttAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGTCAGCAACACTCCCCTCGTGCCGa                                               >  1:421597/1‑149 (MQ=255)

                                                                                      ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACATAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGCTCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCACCACTCCCCTCGTGCAGCGttt                                           >  2:479391/1‑149 (MQ=255)

                                                                                         aagtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAg                                        >  2:241381/1‑149 (MQ=255)

                                                                                                 gCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACACCCCTCGTGCCGAGTTTAAGCGCGGCtt                                >  1:333984/1‑149 (MQ=255)

                                                                                                    ggATATGAAATCGGGCACCTGTCAGACTTAAGGCTGCCCCACTGTGAGCGGTTTCGCCAAGTCTATGATCTTAAGATATGGGTCTTTTGCCTTAACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCGGGGTTTTAGCCCGGCTTCaa                             <  2:408652/149‑1 (MQ=255)

                                                                                                     tatatGAAATCCGGCATCTTTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGCTAGGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACTCTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                            <  2:363273/148‑1 (MQ=255)

                                                                                                     gATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                            <  2:494478/149‑1 (MQ=255)

                                                                                                        aggAAATCCGGCACCTGTCCGACTTAAGGCTGCCCCGCCGCGAGCGTTTTCGCTAATTCTAAAATCTTATGATCGGGCGCTTGTGCCTCCACCGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTc                         <  2:44907/147‑1 (MQ=255)

                                                                                                          gAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAACGCGGCTTCAACGTCct                       >  1:11617/1‑149 (MQ=255)

                                                                                                          gAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTATCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTACGCGCGGCTTCCACGTccc                       >  2:247596/1‑148 (MQ=255)

                                                                                                           aaaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctc                      >  2:398093/1‑149 (MQ=255)

                                                                                                                    aCCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCtctc             >  1:198386/1‑149 (MQ=255)

                                                                                                                      cTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCaa           <  1:113890/149‑1 (MQ=255)

                                                                                                                         tCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAc        <  2:259513/149‑1 (MQ=255)

                                                                                                                           aGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAccc      >  1:16692/1‑149 (MQ=255)

                                                                                                                               ttAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCCCGGCTTCAACGTCCTCTCCGCTCTCAATACcccccc  >  2:104702/1‑149 (MQ=255)

ACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCC  >  NC_000913_3_pae_tpiA/4110613‑4110888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A