Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F6 I1 R1
|
16 |
39.4 |
1365814 |
93.3% |
1274304 |
143.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_pae_tpiA |
4,110,747 |
G→T |
intergenic (+62/+784) |
cdh → / ← yiiQ |
CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_pae_tpiA | 4,110,747 | 0 | G | T | 97.2%
| 111.6
/ NA
| 36 | intergenic (+62/+784) | cdh/yiiQ | CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (21/14); total (22/14) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
CAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGA‑AATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAG > NC_000913_3_pae_tpiA/4110611‑4110891
|
caACGATATCTACTGACGCTTAACCATGCTTCAGCCGAGGA‑AATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAACGGTTAAGGAAAGTAAGTGCCAGATATGAAATCCGGCACCTGACAGACTTAAGGCTGCCCCGCCGCGa < 1:617913/149‑1 (MQ=255)
gAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGA‑AATTCAGGATCATCAGTGTGAGATCTTGCGTTCAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGAATAAAGGCTGCCCCGCCGCGAGAgg > 1:193079/1‑149 (MQ=255)
tACTGACGCTTACCCGTGCTTCAGCCGAGGA‑AATTCAGGATCATCAGTGGGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAACCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGc < 1:207151/149‑1 (MQ=255)
tGACGCTTAACCGTGCTTCAGCCGAGGA‑AATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCaa < 1:128223/149‑1 (MQ=255)
tGACGCTTAACCGTGCTTCAGCCGAGCACAACACA‑GATCAGCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCaa > 1:165578/1‑149 (MQ=255)
ttCAGCCGAGGA‑AATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGa > 2:643263/1‑149 (MQ=255)
tCAGCCGAGGA‑AATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGat < 2:109160/149‑1 (MQ=255)
aaTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCtttt < 1:529198/149‑1 (MQ=255)
ggAACAGCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTc > 2:427145/1‑149 (MQ=255)
tgtgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACATGTCAGACTTAAGGCTGCCCCGCCCCGAGCGGTTTCGCCAATTCTAAGGTCTTCACATATGGGTTTTTTGTTTTCACGCGTgcgc > 2:311981/1‑149 (MQ=255)
tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCAAATTCTAAGATCTTAAGATATCGGTCTTTTGCCTTCACGGCTGCGCtt > 2:169119/1‑149 (MQ=255)
tttGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAga < 2:120480/149‑2 (MQ=255)
aaGGCGGAGAGGTAAGGAGAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCAGCAAGCGGTTTCGCCAGTTCTAAGATCTTAAGATATGGGTCTTTTTCCTTAACGCCTACGCTTGCCAGCAACAcccc > 2:231608/1‑149 (MQ=255)
ggCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTcccc < 2:389131/149‑1 (MQ=255)
gAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCg > 1:390194/1‑149 (MQ=255)
ttAAGGAAAGTAAGTGCCCGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGa < 1:407713/149‑1 (MQ=255)
aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGt > 1:404108/1‑149 (MQ=255)
aGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGAAACCCTCCCCTCGTGCCGAGtt > 1:312100/1‑149 (MQ=255)
gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACCCTCCTCTCGTGCCGAGTTTa < 2:527115/149‑1 (MQ=255)
taagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAGCACTCCCCTCGTGCCGAGTTTAAgcgc < 1:101340/149‑1 (MQ=255)
gtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGc < 1:643263/149‑1 (MQ=255)
cGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCTCTTCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCa > 1:500208/1‑149 (MQ=255)
gagacgAATTCCGGCATGGGTCAGACTTAAGGGTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCTTTCACGGCTGCGCTTGTCCGCAACACTCCCCTCGTGCTGAGTTTAAGCGCGGCTTTAAc < 2:181314/144‑1 (MQ=255)
gAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGACCTGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCct > 1:606979/1‑149 (MQ=255)
cACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctct < 2:404108/149‑1 (MQ=255)
aCCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGcgccc > 1:241244/1‑145 (MQ=255)
aCCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCtctc > 2:327529/1‑149 (MQ=255)
ccTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCa > 1:357890/1‑149 (MQ=255)
cTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGATTAAGCGCGGCTTCAACGTCCTCTCCCCTCTCaa > 1:285522/1‑149 (MQ=255)
gTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAAtc > 2:470590/1‑148 (MQ=255)
gTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCCTCAACGTCCTCTCCGCCCTCAAtc > 2:376668/1‑148 (MQ=255)
cAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTACGATATAAAGACATTGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAcc < 1:192110/149‑1 (MQ=255)
aGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCACTCGTGCCGAGTTTAAGCGCGGCGTCAACGTCCTCTCCCCTCTCAATAccc > 2:524911/1‑149 (MQ=255)
aCTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGACTTCACGGCTGCGCTTGCCAGCAACCGTAACATCGTGCAGGGTTGAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGc > 2:275/1‑149 (MQ=255)
cTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCACTCGTGCCGAGTTTAAGCGCAGCTTCAACGTCCTCTCCGCTCTCAATACCCGcc > 1:67174/1‑149 (MQ=255)
aGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCACTCTACATACCAGCCCCAg > 1:585510/1‑149 (MQ=255)
|
CAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGA‑AATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAG > NC_000913_3_pae_tpiA/4110611‑4110891
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
