Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F23 I1 R1	16	57.8	2174870	95.1%	2068301	133.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,110,747	G→T	intergenic (+62/+784)	cdh → / ← yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,110,747	0	G	T	95.4%	301.2 / 3.0	87	intergenic (+62/+784)	cdh/yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand): ref base G (1/3); new base T (41/42); total (42/45)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCA  >  NC_000913_3_pae_tpiA/4110600‑4110890

tGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAggctgc                                                                                                                                               <  3:93171/150‑1 (MQ=255)

    ggCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCccg                                                                                                                                           >  2:187407/1‑150 (MQ=255)

         cacaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCcgcg                                                                                                                                      <  1:436200/150‑1 (MQ=255)

          acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGa                                                                                                                                     >  1:139148/1‑150 (MQ=255)

           caACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGTGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAg                                                                                                                                    <  3:410837/150‑1 (MQ=255)

            aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGc                                                                                                                                   <  2:191883/150‑1 (MQ=255)

            aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGc                                                                                                                                   >  2:77835/1‑150 (MQ=255)

             aCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATNCAGGAACATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGGTAAGGAAAGTAAGTGCCGGATATGAAAACCGTCAAATGTCAGACTTAACGCTGCCCCGCGGNGAGCg                                                                                                                                  >  1:253001/1‑150 (MQ=255)

              cGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCgg                                                                                                                                 >  2:606918/1‑150 (MQ=255)

               gAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGt                                                                                                                                >  1:315910/1‑150 (MQ=255)

                  aTCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTc                                                                                                                             <  2:426281/150‑1 (MQ=255)

                     tACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGcc                                                                                                                          <  2:511597/150‑1 (MQ=255)

                        tGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAAt                                                                                                                       >  3:226739/1‑150 (MQ=255)

                            gCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTa                                                                                                                   >  2:205002/1‑150 (MQ=255)

                             cTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTaa                                                                                                                  >  1:556478/1‑150 (MQ=255)

                                    gTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTa                                                                                                           <  2:570084/150‑1 (MQ=255)

                                          cAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGatat                                                                                                     <  1:606918/150‑1 (MQ=255)

                                             ccGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATggg                                                                                                  >  2:340978/1‑150 (MQ=255)

                                              cGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGt                                                                                                 >  2:96527/1‑150 (MQ=255)

                                              cGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGt                                                                                                 <  2:315910/150‑1 (MQ=255)

                                               gAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTc                                                                                                >  4:256621/1‑150 (MQ=255)

                                               gAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTc                                                                                                <  1:96527/150‑1 (MQ=255)

                                                aGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCt                                                                                               >  4:115295/1‑150 (MQ=255)

                                                aGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCt                                                                                               >  1:327788/1‑150 (MQ=255)

                                                 ggAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCtt                                                                                              >  3:301936/1‑150 (MQ=255)

                                                  gAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCttt                                                                                             >  1:426114/1‑150 (MQ=255)

                                                  gAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCttt                                                                                             >  3:174749/1‑150 (MQ=255)

                                                  gAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCttt                                                                                             >  3:174788/1‑150 (MQ=255)

                                                       tCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGTCACCTGGCAGACTTAATGCTGCCCCGCCGCGAGCGGTTTCGGCAATTCTAAGATCTTATGATATGGGTCTTTTGCCt                                                                                        >  1:671370/1‑150 (MQ=255)

                                                         aGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTc                                                                                      >  1:157020/1‑150 (MQ=255)

                                                         aGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTc                                                                                      <  4:124865/150‑1 (MQ=255)

                                                          ggATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCa                                                                                     <  1:516295/150‑1 (MQ=255)

                                                          ggATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCa                                                                                     <  1:27088/150‑1 (MQ=255)

                                                           gATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCAc                                                                                    <  1:614470/150‑1 (MQ=255)

                                                            atcatcAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACg                                                                                   >  4:362985/1‑150 (MQ=255)

                                                             tcatcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACgg                                                                                  <  2:626898/150‑1 (MQ=255)

                                                                tcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGACGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTg                                                                               <  2:28213/150‑1 (MQ=255)

                                                                  aGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgcg                                                                             >  3:365544/1‑150 (MQ=255)

                                                                   gtgtGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgcgc                                                                            <  3:325201/150‑1 (MQ=255)

                                                                      tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACgg                                                                                  >  1:638567/1‑141 (MQ=255)

                                                                      tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACgg                                                                                  <  2:638567/141‑1 (MQ=255)

                                                                      tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTg                                                                         >  1:305838/1‑150 (MQ=255)

                                                                       gagaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGc                                                                        <  4:107484/150‑1 (MQ=255)

                                                                        agaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTGCGCCGATTCTAAGATCTTAAGAGATGGGTCGTTTGCCTTCACGGCTGCGCTTGcc                                                                       >  3:232896/1‑150 (MQ=255)

                                                                         gaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCa                                                                      <  1:187407/150‑1 (MQ=255)

                                                                         gaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCa                                                                      <  1:491303/150‑1 (MQ=255)

                                                                           ttttGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGc                                                                    >  2:420907/1‑150 (MQ=255)

                                                                                   tAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTcc                                                            <  2:139148/150‑1 (MQ=255)

                                                                                    agggCGAAGAGTTAAGGACAGTAAGTGCCGGACATGAAATCCGGGACGTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTccc                                                           <  4:328772/148‑1 (MQ=255)

                                                                                              gTTAAGGAAAGTAAGCGCCGGATAAGAAATCCGGCACCTGTCAGACATACGGCGGCCCCGCCGCGAGCGGTTGCGCGAATTGTAAGTGCTTAAGATATGGGTCGGTGGCCATCAGGGCGGCGCCTGCCAGCAACGCTCCCCACGTGCCGa                                                 <  2:354569/150‑1 (MQ=255)

                                                                                                 aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCg                                                                                                                            <  2:251648/72‑1 (MQ=255)

                                                                                                 aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCg                                                                                                                            >  1:251648/1‑72 (MQ=255)

                                                                                                 aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCaa                                                                                                                        >  2:353066/1‑76 (MQ=255)

                                                                                                 aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCaa                                                                                                                        <  1:353066/76‑1 (MQ=255)

                                                                                                   ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCGCGCCGCGAGCGTTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCGTTCCAGCAACACTCCCCTCGTGCCGAGTTTa                                            >  2:401729/1‑150 (MQ=255)

                                                                                                   ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTa                                            <  1:420907/150‑1 (MQ=255)

                                                                                                    gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTaa                                           <  2:365200/150‑1 (MQ=255)

                                                                                                    gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTaa                                           >  2:18323/1‑150 (MQ=255)

                                                                                                    gAAAGTAAGTGCCGGATATGAAATCCGGCACATGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTaa                                           <  2:253001/150‑1 (MQ=255)

                                                                                                         taagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGCTCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGCGTTTAAGcgcg                                      <  2:671370/150‑1 (MQ=255)

                                                                                                         taagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGcgcg                                      <  3:115295/150‑1 (MQ=255)

                                                                                                          aagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCgg                                     >  4:184656/1‑150 (MQ=255)

                                                                                                            gtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCt                                   <  4:174749/150‑1 (MQ=255)

                                                                                                            gtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCt                                   <  4:174788/150‑1 (MQ=255)

                                                                                                             tGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCtt                                  >  4:185591/1‑150 (MQ=255)

                                                                                                              gCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTc                                 <  1:205002/150‑1 (MQ=255)

                                                                                                                cGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCaa                               >  1:235296/1‑150 (MQ=255)

                                                                                                                 ggATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                              <  2:426114/150‑1 (MQ=255)

                                                                                                                 ggATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                              <  4:53724/150‑1 (MQ=255)

                                                                                                                     atGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTcc                          >  3:242176/1‑150 (MQ=255)

                                                                                                                        aaaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGcc                                                                       <  1:9899/102‑1 (MQ=255)

                                                                                                                        aaaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGcc                                                                       >  2:9899/1‑102 (MQ=255)

                                                                                                                        aaaTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                       >  4:336893/1‑150 (MQ=255)

                                                                                                                          aTCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTcc                     <  3:336893/150‑1 (MQ=255)

                                                                                                                          aTCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTcc                     <  1:18323/150‑1 (MQ=255)

                                                                                                                             cGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGct                  <  3:206780/150‑1 (MQ=255)

                                                                                                                               gCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctct                <  3:185591/150‑1 (MQ=255)

                                                                                                                                cACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCtctc               <  3:9251/150‑1 (MQ=255)

                                                                                                                                   cTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAAt            <  1:168950/150‑1 (MQ=255)

                                                                                                                                     gTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAc          >  2:552507/1‑150 (MQ=255)

                                                                                                                                     gTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAc          >  2:101744/1‑150 (MQ=255)

                                                                                                                                     gTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAc          >  4:209731/1‑150 (MQ=255)

                                                                                                                                      gcAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAcc         <  2:640238/149‑1 (MQ=255)

                                                                                                                                        aGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGc                                                                        <  1:233039/85‑1 (MQ=255)

                                                                                                                                        aGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGc                                                                        >  2:233039/1‑85 (MQ=255)

                                                                                                                                        aGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCg       >  1:454645/1‑150 (MQ=255)

                                                                                                                                             tAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCa  <  3:184656/150‑1 (MQ=255)

                                                                                                                                             tAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCa  >  3:61460/1‑150 (MQ=255)

TGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCA  >  NC_000913_3_pae_tpiA/4110600‑4110890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A