Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F17 I0 R1 98 90.7 2214160 82.3% 1822253 222.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3610C.79.6% 134.0 / 28.3 49pseudogene (2/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (3/7);  new base . (38/1);  total (41/8)
Fisher's exact test for biased strand distribution p-value = 1.05e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.
*NC_0009132,173,3620C.79.7% 135.8 / 26.4 49pseudogene (1/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (3/6);  new base . (38/1);  total (41/8)
Fisher's exact test for biased strand distribution p-value = 4.50e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

GCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCA  >  NC_000913/2173129‑2173599
                                                                                                                                                                                                                                         ||                                                                                                                                                                                                                                             
gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgt                                                                                                                                                                                                                                          >  1:633528/1‑234 (MQ=255)
                    aCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGCTTACCCGGCACACAAACAGCAATT‑AAACTGGTGAGTGGGATACAAATCAGGCTTGCCGCTACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCg                                                                                                                                                                                                                    >  1:144060/1‑239 (MQ=255)
                    aCCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGAGAGTGGGATAAAAATCAGGCTTGCCGAACCCACCCC‑CC‑TATGGCCCAGCACCAACCCCg                                                                                                                                                                                                                    >  2:281989/1‑239 (MQ=255)
                              tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGAAGGTCCCAGACGCAACGCCGGATCCAGGcc                                                                                                                                                                                                          >  1:789039/1‑239 (MQ=255)
                                cGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACCAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGGTACCACCC‑‑CCGAATGTCCCAGCAGCAACGCCGGCCCAAGACCaa                                                                                                                                                                                                        >  1:497400/1‑239 (MQ=255)
                                               ggTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGCTAAAAATCAGGCTTGCCGATACCACCC‑‑CCGTAGTGCCCAGCAGAAACGCCGGGTCAAGGCAAATCAGGAACTCTTGa                                                                                                                                                                                          >  1:623611/1‑238 (MQ=255)
                                                  gATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAACCAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCCGCAACGCCGGTTCAAGGCCAATCAGGAAATCCTGAccg                                                                                                                                                                                       >  1:667450/1‑238 (MQ=255)
                                                    tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGGGGGATAACAATCAGGCTTGCCGCTACCA‑‑CCGCCGTATGTCCCAGCAGAAACCCCGGCTCAAGGCCAATCAGAAACCCCTTAccccca                                                                                                                                                                                    >  2:528387/1‑238 (MQ=255)
                                                         aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGCCCAATCAGGAATCCCTGACCCCCGAACt                                                                                                                                                                                >  1:368320/1‑238 (MQ=255)
                                                           aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGAATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCCGGAATCCCTGACCGCCGAACTTc                                                                                                                                                                              >  1:847330/1‑238 (MQ=255)
                                                               atgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATCAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGCCCCAGCAGCAACGCCGGATCAAGGCCAAAACGAAACCCCTACCCCCCGAACtccccccc                                                                                                                                                                         >  2:116474/1‑232 (MQ=255)
                                                                tgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGt                                                                                                                                                                        >  1:124090/1‑239 (MQ=255)
                                                                tgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGt                                                                                                                                                                        >  1:36666/1‑239 (MQ=255)
                                                                tgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACAA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGCCGCCCGAACTTCGCCCGt                                                                                                                                                                        >  1:420625/1‑239 (MQ=255)
                                                                     cccGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACg                                                                                                                                                                   >  1:350049/1‑239 (MQ=255)
                                                                     cccGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGAATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTGGCCTTGAAAc                                                                                                                                                                    >  1:804400/1‑238 (MQ=255)
                                                                         agaTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACt                                                                                                                                                                >  1:333582/1‑238 (MQ=255)
                                                                         agaTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGGATGTCCCAGCAGCAACGCCGGGTCAAGGCCAACCAGGAACCCCTGACCCCCCACCTTCACCGTGAAACGAcaa                                                                                                                                                               >  2:797806/1‑237 (MQ=255)
                                                                              aaGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGACCCAGAAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACCAg                                                                                                                                                           >  1:1099064/1‑238 (MQ=255)
                                                                              aaGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGGGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAg                                                                                                                                                           >  1:16020/1‑238 (MQ=255)
                                                                                ggTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTACACGACTACGAg                                                                                                                                                           >  1:42218/1‑236 (MQ=255)
                                                                                ggTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGcc                                                                                                                                                         >  1:632838/1‑238 (MQ=255)
                                                                                      gAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGAAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTa                                                                                                                                                   >  1:544466/1‑238 (MQ=255)
                                                                                            aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCACGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTCCAGGCCTGCTTaaggctgg                                                                                                                                            >  1:994096/1‑232 (MQ=255)
                                                                                                     aTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgt                                                                                                                                                                                                                                          >  1:338002/1‑133 (MQ=255)
                                                                                                     aTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgt                                                                                                                                                                                                                                          <  2:338002/138‑6 (MQ=255)
                                                                                                      tGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGCTAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAACCTGCTTAACGATGGGCGTTAAAcc                                                                                                                                  >  1:806140/1‑239 (MQ=255)
                                                                                                      tGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcc                                                                                                                                  >  1:872935/1‑239 (MQ=255)
                                                                                                                ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa                                                                                                                                    >  1:781659/1‑227 (MQ=255)
                                                                                                                ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTACCGATGGGCGTTCAACCATCCatgatg                                                                                                                        >  1:554703/1‑239 (MQ=255)
                                                                                                                                 agcccgtgggtggAAAGATCCCAAAGCGGCAGCCTCTTATTCCCCCGCACAAAAACAAAAATA‑AAAATGG‑GAGGGGGGAAAAAAACCGGGTTGCGCAAACC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATAAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTgg                                                                                                       <  2:333582/231‑1 (MQ=255)
                                                                                                                                       aTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGGAAACGACTACGAGCCTGCTTAGCGATGGACGTTAAACCATCCATGATGGGTTTAATCACCCGTGTCATTa                                                                                                  >  1:1045653/1‑238 (MQ=255)
                                                                                                                                        ggggggaaaGATCCCCAAAGGGCAGCCCCTGATTCCCCGGCACACAAACAGAAATT‑AAAATGGTGAGTGGGATAAAAATCGGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTag                                                                                                 <  1:281989/231‑1 (MQ=255)
                                                                                                                                             gCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGGCGAACTTCGCCTGCAAACGACTACGAGCCTGCTTTGCGCTGGGCGTTAAACCATCCATAATGGGCTTAATCAACCGGGGCATTAgaagcaa                                                                                           >  2:806657/1‑238 (MQ=255)
                                                                                                                                                     gCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCCTGATGGGTTTAATCACCCGTGGCATTACCAGCATACCTgcc                                                                                    >  1:282450/1‑238 (MQ=255)
                                                                                                                                                                     ttaaccccccGCACACAAACAAAAATA‑AAGGGGGTGGGTGGGAAAAAAAGCGGGC‑‑GGCGCGACCCCCCCGCCGTTGGCCCCAACAGAACCCCGGGAAAAAGGCAAATCGGGAACCCCGGACCGCCGAACTTCGCCTGTAAACGACGACGAGCCTGCTTAGCGATGGGCGTTAACCCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAg                                                                   <  2:554703/233‑1 (MQ=255)
                                                                                                                                                                      gatgccccGGCCCAAAAACAAAAATA‑AAAAGGGTTGGTGGAACAAAAATCGGGCTCGCCG‑‑ACCCCACCGCCGTATGCCCCAGCAGCACGGCCGGATCAAGGCCAATCAGCAACTCTTGACCGCCGAACTTCGCCGGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGc                                                                  <  2:124090/234‑1 (MQ=255)
                                                                                                                                                                             ggcaccacaAACA‑CAATAAAAAAGGGGGAGGGGTAAAAAAACCAGGTTTGCAGAT‑CC‑CCCCGCCGTATGGCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATa                                                           <  1:116474/234‑1 (MQ=255)
                                                                                                                                                                              ggCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATac                                                          >  1:1019173/1‑239 (MQ=255)
                                                                                                                                                                              ggCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATac                                                          >  1:215045/1‑239 (MQ=255)
                                                                                                                                                                              ggCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATAACTGCCGCCGTTTTTACCGCAAGCTGCAATa                                                           >  1:701254/1‑238 (MQ=255)
                                                                                                                                                                                   acaAACAGAAATT‑AAAATGGGAGGTGGGAAAAAAATCAGGTTTGCCAATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCtt                                                     <  1:57623/239‑1 (MQ=255)
                                                                                                                                                                                      aaaggCAATT‑AAATGGGGGAGGGGAAAAAAAGCCAGTCTGGCGGA‑ACC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAGCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGa                                                  <  1:85649/235‑1 (MQ=255)
                                                                                                                                                                                          gCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTCCACCCCTTTTTACCCCCCCCCCCAAAACACCTTTGaaaac                                              >  2:85649/1‑235 (MQ=255)
                                                                                                                                                                                             aTT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTa                                           >  1:992637/1‑239 (MQ=255)
                                                                                                                                                                                             aTT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTa                                           >  1:237798/1‑239 (MQ=255)
                                                                                                                                                                                               t‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCCCCGTTTTTACCCCCAGCTGCAATAAACCTTTGACacccaaa                                          >  2:1021093/1‑232 (MQ=255)
                                                                                                                                                                                                    aTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTCCCGTCTGTTTTACCACCTGCTGCAATACACCTTTGCCACACTAACCcgc                                     >  1:347962/1‑239 (MQ=255)
                                                                                                                                                                                                         gAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGa                                >  1:621044/1‑239 (MQ=255)
                                                                                                                                                                                                         gAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCAGCCG‑TTTTACCGCCAGCTGCAATACCCCTTTGCAATCGTAAACCCCCAGGa                                >  1:159708/1‑238 (MQ=255)
                                                                                                                                                                                                          aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTATCACTGGGCGTTAAACCATCCCTGATGGGTTTAATCACCCGTGGCCTTCGCAGCATCCCTCCCCCCGTTTTTCCGACCCGCGGCCAACCCCCTTTTACACCGTAAccccgcgggc                                >  2:943434/1‑231 (MQ=255)
                                                                                                                                                                                                                  aaaaaGCGGTCTTGCCGAT‑CC‑CCCCGCCGTATGTCCCAGCAACAACGGCGGATCAAGGCCAATCAGGACCTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATaa                       <  1:797806/239‑1 (MQ=255)
                                                                                                                                                                                                                                         ccccGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAACCGGGAACCCCTGACCGCCGAATTTCGCCTTTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCCCCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCa  <  1:63373/239‑1 (MQ=255)
                                                                                                                                                                                                                                         ||                                                                                                                                                                                                                                             
GCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATT‑AAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCA  >  NC_000913/2173129‑2173599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A