Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I0 R1 91 172.1 3477528 97.2% 3380157 226.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3610C.90.7% 412.1 / 24.4 108pseudogene (2/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (1/9);  new base . (71/27);  total (72/36)
Fisher's exact test for biased strand distribution p-value = 1.82e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.
*NC_0009132,173,3620C.90.8% 407.3 / 19.1 108pseudogene (1/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (0/7);  new base . (71/27);  total (72/36)
Fisher's exact test for biased strand distribution p-value = 2.36e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 113 total aligned reads displayed.

CATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCA  >  NC_000913/2173124‑2173599
                                                                                                                                                                                                                                               ||                                                                                                                                                                                                                                             
cATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCAAAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGCTTTCCACGCTGCACGGCGACCGCCATCCACACGAGGAATCCGATGGAGGCAAGATCGCCAAACAGCAGCACCTGATTACCAGACACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATACAAATCAAGATTACCGATcccacc                                                                                                                                                                                                                                               >  2:1461211/1‑239 (MQ=255)
   tAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATaccaccgcc                                                                                                                                                                                                                                            >  1:1104052/1‑236 (MQ=255)
     gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGCTTAAGGTGCGGAACAGATTTCCACGATGCCCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACACACAGC‑AATTAAACTGGG‑GAGTGGGCTAAAAATCAGGCCTGCCGATaccaccgccgt                                                                                                                                                                                                                                          >  2:1637186/1‑234 (MQ=255)
          cAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGCTTACCCGGCACACAAACAGC‑AATTAAAATGGC‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGt                                                                                                                                                                                                                                    >  1:668997/1‑238 (MQ=255)
          cAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGCTTACCCGGCACACAAACAGC‑AATTAAAATGAT‑GAGTGGGCTAAACATCAGGCTTGCCGATacaaccaccgtatgc                                                                                                                                                                                                                                      >  2:98100/1‑229 (MQ=255)
          cAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGt                                                                                                                                                                                                                                    >  1:282560/1‑238 (MQ=255)
                      cAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAAc                                                                                                                                                                                                                        >  1:462874/1‑238 (MQ=255)
                       aacccAATAGTTGGAGTCGCGATCCACAGGGTGATCCTAATAATGAGGACACCCGGAATTAAGGTGGGAAACAGATTTCCACGATGCACGGCGACCGCCACCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGc                                                                                                                                                                                                                      <  2:462874/236‑1 (MQ=255)
                       aaaCCAATAGTTTGGGTGGCGACCCACAGGGTAATGCGCAAAATGATGCACCCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCCCGGCGCCCGCCATCGCCACGAAGAAGCCGATGGGGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGc                                                                                                                                                                                                                      <  2:1290626/239‑1 (MQ=255)
                                  ttGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCCCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                                                                                                                           <  1:1603778/239‑1 (MQ=255)
                                   tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGcc                                                                                                                                                                                                          >  2:677742/1‑239 (MQ=255)
                                     cGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCaa                                                                                                                                                                                                        >  1:1291518/1‑239 (MQ=255)
                                     cGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCaa                                                                                                                                                                                                        >  1:226960/1‑239 (MQ=255)
                                        cgcgATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCa                                                                                                                                                                                                     >  1:1527298/1‑239 (MQ=255)
                                         gcgATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg                                                                                                                                                                                                    >  1:421929/1‑239 (MQ=255)
                                          acagtcacaGGGTAATGTTAATAAGGATCCCACCCGAGATTAAGGTGCGGAACAGATTTCACCGATGCCTGGCACCCGCCAGCCGCACAAAGAAGTCGAGGGTGGCACGACCGCCAAACGGTGGCACCCGATAACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGAAAAAAATCAGGCTTGCCGAAACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCGGTATCACGGCCAACCAgg                                                                                                                                                                                                   <  2:783160/234‑1 (MQ=255)
                                                    ggTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGa                                                                                                                                                                                          >  1:288605/1‑238 (MQ=255)
                                                        aTGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgcc                                                                                                                                                                                     >  2:731819/1‑239 (MQ=255)
                                                        aTGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgcc                                                                                                                                                                                     >  1:188516/1‑239 (MQ=255)
                                                         tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGCCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGAACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGACCAAGGCCAATCCGGAACTCCTGAccgccg                                                                                                                                                                                    >  2:234667/1‑239 (MQ=255)
                                                         tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTTCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAcggccg                                                                                                                                                                                    >  2:486034/1‑239 (MQ=255)
                                                         tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGAACACAAACAGC‑AATTAAAATGGA‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAAGCCAATCAGGAACTCCTGAccgcc                                                                                                                                                                                     >  2:270486/1‑238 (MQ=255)
                                                                aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTc                                                                                                                                                                              >  2:634033/1‑238 (MQ=255)
                                                                 atgatgACACCCGAGATTAAGGGGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc                                                                                                                                                                            <  1:402787/239‑1 (MQ=255)
                                                                      gaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTa                                                                                                                                                                       >  1:1493305/1‑239 (MQ=255)
                                                                      gaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTa                                                                                                                                                                       >  1:200335/1‑239 (MQ=255)
                                                                         cacagagagaaaGGGGCGGAAAGAATTCCCACGAGCCCCGGCAGCCGCCATCGCCACGAAGAGGACGATGGTGGCAAGAGCGCAAAACGGCAGCATCTAATTGCCCGTCACACAAACAGC‑AATAAAAAGGTT‑GAGTGGGATAAAACTCAGGCTTGCCAATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAAc                                                                                                                                                                    <  2:770857/229‑1 (MQ=255)
                                                                          cccGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACg                                                                                                                                                                   >  2:458577/1‑239 (MQ=255)
                                                                           ccGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACg                                                                                                                                                                   >  1:1563285/1‑238 (MQ=255)
                                                                               gaTTAGGGTGCGGAACAGATTCCCCCGATGCACGGCGACGGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGACAGCCCCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGCCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTa                                                                                                                                                               <  2:1547412/238‑1 (MQ=255)
                                                                                   aggcgccGGAACAGATTGCCGCGAGCCCCGGCACCCCCCACCGCCACGAAGAAGCCGAGGGTGGCAAGCTCGCCAAACGGCAGCACCTGTTTACCCGGCACACAAACAGA‑AATTAAAATGGT‑GAGTGGGAAAAAAACCGGGCTTGCCGAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                                                                                                                          <  2:654485/233‑1 (MQ=255)
                                                                                         cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg                                                                                                                                                                                                   <  1:1727264/192‑1 (MQ=255)
                                                                                         cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg                                                                                                                                                                                                   >  2:1727264/1‑192 (MQ=255)
                                                                                         cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCtt                                                                                                                                                    >  1:190539/1‑239 (MQ=255)
                                                                                         cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCtt                                                                                                                                                    >  2:766155/1‑239 (MQ=255)
                                                                                         cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCtt                                                                                                                                                    >  1:850744/1‑239 (MQ=255)
                                                                                         cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCtt                                                                                                                                                    >  1:277081/1‑239 (MQ=255)
                                                                                         cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCtt                                                                                                                                                    >  2:1552817/1‑239 (MQ=255)
                                                                                          ggAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGCGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGCCCAATCAGGAACTCCTGCCCGCCGAACTTCGCCCGTAAACGCGTACGCACCTGCTTa                                                                                                                                                   >  2:937138/1‑239 (MQ=255)
                                                                                               gaatccccgcGATGCCCGGCACCCGCCCCCCCCCCGAGAAGGCCGATGGGGGAAAAACCGCAAACCGGCAGCACCTGATTCCCCGGAAAAAAAACAGA‑AATAAAAAGGGT‑GAGGGGGATAAAAATCAGGCTGGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                              <  2:190539/230‑1 (MQ=255)
                                                                                               aGATTCCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                              <  1:1628334/239‑1 (MQ=255)
                                                                                               aGATTCCCACGAGGCCCGGCGACCGCCACCGCCACGAAGAAGCCGATGGTGGCAAGATCGCGAAACGGCAGCACCTGATTACCCGGCACACAACCAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                              <  1:766656/239‑1 (MQ=255)
                                                                                                 aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATgg                                                                                                                                            >  1:1212439/1‑239 (MQ=255)
                                                                                                 aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATgg                                                                                                                                            >  1:1358373/1‑239 (MQ=255)
                                                                                                    tCCACGATGCACGGCGACCACCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATA‑‑ACACCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAAATCCTGACAGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCg                                                                                                                                         >  1:521550/1‑239 (MQ=255)
                                                                                                    acccagaggcccGGCTACCGCCACCCCACCAAAGATGCCAAGGGCGGAAAGACCGCCAAAGGGCAGCCCCTAATTACCCGGCACACAAACAGA‑AATAAAAATGGT‑GGGGGGAATAAAAATCAGGTTGCCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCg                                                                                                                                         <  2:288605/228‑1 (MQ=255)
                                                                                                      cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg                                                                                                                                                                                                   >  2:891640/1‑179 (MQ=255)
                                                                                                      cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg                                                                                                                                                                                                   <  1:891640/179‑1 (MQ=255)
                                                                                                      cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGAAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGtt                                                                                                                                       <  1:731819/239‑1 (MQ=255)
                                                                                                          gtgccgggcacccGCAATGGCCACAAAAAGGCGGAGGGGGGAAAGACCGCAAAAGGGCAGCACGTGATACCCCGGCACACAAACAGA‑AATAAAAAGGGG‑GAGGGGGAAAAAAACCGGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa                                                                                                                                    <  2:347887/227‑1 (MQ=255)
                                                                                                           tGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGa                                                                                                                                                            >  1:113870/1‑213 (MQ=255)
                                                                                                           tGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                                                                                                                                   <  1:1311628/238‑1 (MQ=255)
                                                                                                            gccccgcaaCCGCCAGCGCCCCGAAGACGCGCTGGGTGGCAAGATCGCAAAACGGCAGCACCTTATTACCCGCCACACAAACAGC‑AATTAAAATGGT‑GAGGGGGACAAAAACCAGGTTTGCCGAGACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGTTCAAGGCAAAGCAGGAACTCCTGACCGCCGAACTTCGCCGGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcca                                                                                                                                 <  2:1375601/231‑1 (MQ=255)
                                                                                                            ccccGGCGACCGCCAGCGCCACGAAGAAGCCGATGGTGGAAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcca                                                                                                                                 <  2:896438/236‑1 (MQ=255)
                                                                                                             cccGGCGCCCGCAACCGCCACGAAGAAGCCGAGGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAACCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcca                                                                                                                                 <  1:1307566/236‑1 (MQ=255)
                                                                                                             cACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcca                                                                                                                                 >  1:1487220/1‑238 (MQ=255)
                                                                                                                     ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAAATTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGAGCGTTAAACCATCCatgatg                                                                                                                        >  1:264342/1‑239 (MQ=255)
                                                                                                                                  gaaaaaGCCGTAGGTGGCAAGATCGCCAAACGGCAGCACCTGATTCCCCGGCACACAAACAGC‑AATAAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAccc                                                                                                           <  1:1454132/239‑1 (MQ=255)
                                                                                                                                  gaaaaaCCCGATGGTGGCAAGATCGCCAAACGGCACCACCGGATTCCCCGGCCCACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAACCAGGCGTGCCGAT‑CC‑CTCCGCCGTATGTCCCAGCACCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAccc                                                                                                           <  1:104567/239‑1 (MQ=255)
                                                                                                                                   aagaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAccc                                                                                                           <  1:677742/238‑1 (MQ=255)
                                                                                                                                         ccGAGGGTGGAAAGATCGCCAACGGGCAGACCGTAATTACCCGGCACACAAACAGC‑AATTAAAATGGG‑GGGTGGGAAAAAATGCAGCTTTGCCGAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCa                                                                                                     <  2:1527298/238‑1 (MQ=255)
                                                                                                                                              ggtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTggca                                                                                               >  2:490521/1‑239 (MQ=255)
                                                                                                                                                  gCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAATTGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAgcagca                                                                                            >  1:78243/1‑238 (MQ=255)
                                                                                                                                                  gCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt                                                                                                                >  1:668732/1‑218 (MQ=255)
                                                                                                                                                           ccATACGGCATGACCTATTTACCCGGCGCAAAAAGAGC‑AATT‑AAATGGTGGAGGGGGAAAAAAACCAGTCTGCCCGATACCA‑‑CCGCCTTATGTCCCAGCGGCACCGCCGGATCAAGGCCAAGCAGGAACCCCTGACCGCCGAACTTCGCCTGTAACCGACTCCGGCCCTGCTTTGCGAGGGGCGTTAAACCATCCATGATGGGTTTAATCGCCCGTGGCATTAGCAGCATCATTgccgc                                                                                  <  2:78243/239‑1 (MQ=255)
                                                                                                                                                                   cagcaCCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg                                                                                                                                                                                                   <  1:953281/118‑1 (MQ=255)
                                                                                                                                                                   cagcaCCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg                                                                                                                                                                                                   >  2:953281/1‑118 (MQ=255)
                                                                                                                                                                          tGATTCCCGGGCACACAAACAAA‑AATTAAAATGGG‑GGTTGGGATAAAAACCAGCCTTGCCGAAAC‑‑CCCCGCCGTATGCCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCCCCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAg                                                                   <  2:1493305/239‑1 (MQ=255)
                                                                                                                                                                           gATTACCCGGCACACAACCAGA‑AATAAAAGTGGT‑GAGGTGGATAAAAACCAGCCTGGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGc                                                                  <  1:384929/239‑1 (MQ=255)
                                                                                                                                                                           aaTTACCCGACACACAAACAGC‑AATTAAAGTGGT‑GAGTGGGAAAAAAACCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGc                                                                  <  1:804416/238‑1 (MQ=255)
                                                                                                                                                                                   ggCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATac                                                          >  1:1394117/1‑239 (MQ=255)
                                                                                                                                                                                   ggCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATac                                                          >  1:1537810/1‑239 (MQ=255)
                                                                                                                                                                                   ggCACAAAAACAGCAAATTAAAGGGGT‑GGGGGGAAAAAAAACAAGGCTGGCTGAAACCA‑‑CGGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCAAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATa                                                           <  2:298364/239‑1 (MQ=255)
                                                                                                                                                                                     cacacaAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATacc                                                         >  1:1593941/1‑237 (MQ=255)
                                                                                                                                                                                      acacaAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATaca                                                         >  1:1069945/1‑237 (MQ=255)
                                                                                                                                                                                      acacaAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATaca                                                         >  1:298364/1‑237 (MQ=255)
                                                                                                                                                                                      acacaAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATaca                                                         >  1:548887/1‑237 (MQ=255)
                                                                                                                                                                                      acacaAACAGC‑AATTAAAATGGC‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTTCCGCCAGCTGCAATacc                                                         >  2:1601168/1‑236 (MQ=255)
                                                                                                                                                                                               gC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATc                                              >  1:1356282/1‑239 (MQ=255)
                                                                                                                                                                                                   aTTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGTCATCGTa                                           >  1:1164029/1‑239 (MQ=255)
                                                                                                                                                                                                   aTTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTa                                           >  1:811644/1‑239 (MQ=255)
                                                                                                                                                                                                   aTTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTa                                           >  2:860907/1‑239 (MQ=255)
                                                                                                                                                                                                   aTTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCCGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCg                                                                                                                                                >  1:1111709/1‑138 (MQ=255)
                                                                                                                                                                                                         aTGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCcgc                                     >  2:439566/1‑239 (MQ=255)
                                                                                                                                                                                                           ggT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTCACATCGTAACCCGCGa                                   >  1:546223/1‑239 (MQ=255)
                                                                                                                                                                                                               gAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTg                                                                <  1:832828/207‑1 (MQ=255)
                                                                                                                                                                                                               gAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTg                                                                >  2:832828/1‑207 (MQ=255)
                                                                                                                                                                                                               gAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGa                                >  1:231260/1‑239 (MQ=255)
                                                                                                                                                                                                               gAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGa                                >  1:1392922/1‑239 (MQ=255)
                                                                                                                                                                                                                aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATa                                                           >  1:1504312/1‑211 (MQ=255)
                                                                                                                                                                                                                aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGa                                   >  1:68464/1‑235 (MQ=255)
                                                                                                                                                                                                                  tGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATg                              >  1:287775/1‑238 (MQ=255)
                                                                                                                                                                                                                     gaaaaaaaCCAGTCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCg                           <  1:857034/235‑1 (MQ=255)
                                                                                                                                                                                                                      taaaaaaCCAGGGTTGCCCA‑ATCCCACCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCg                           <  1:1169575/236‑1 (MQ=255)
                                                                                                                                                                                                                               aGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGcc                 >  2:1446243/1‑238 (MQ=255)
                                                                                                                                                                                                                                  cTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCa              <  1:1552817/238‑1 (MQ=255)
                                                                                                                                                                                                                                     gCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcac          >  1:149292/1‑239 (MQ=255)
                                                                                                                                                                                                                                     gCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcac          >  2:1628490/1‑239 (MQ=255)
                                                                                                                                                                                                                                       cGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcaca         <  1:634033/238‑1 (MQ=255)
                                                                                                                                                                                                                                               ccccGCCGTAGGTCCCACCAGAAACGCGGGAGCAAGGCAAACCAGAAACCCCGCACCGCCGACCTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCa  <  2:1487220/239‑1 (MQ=255)
                                                                                                                                                                                                                                               ||                                                                                                                                                                                                                                             
CATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGC‑AATTAAAATGGT‑GAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCA  >  NC_000913/2173124‑2173599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A