Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F35 I0 R1 275 15706.7 10805118 98.3% 10621430 134.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,014,291 G→T 17.4% intergenic (+137/‑207) bolA → / → tig transcriptional regulator BolA/trigger factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,014,2910GT17.4% 171.7 / 3.2 133intergenic (+137/‑207)bolA/tigtranscriptional regulator BolA/trigger factor
Reads supporting (aligned to +/- strand):  ref base G (38/60);  new base T (2/27);  total (40/93)
Fisher's exact test for biased strand distribution p-value = 1.10e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCC  >  NZ_CP022686/4014162‑4014422
                                                                                                                                 |                                                                                                                                   
tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCGTTTTTCCCAGCAGTCAGACGAAAATCGCCCGGGAATTTTTTAAAAATa                                                                                                                             >  3:390869/1‑138 (MQ=255)
    cAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAATGGCCTGCGGGCCGTTTTGTTTTGTCTGGTTTTTGCGCGTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATAAGCGAc                                                                                                                       >  7:582582/1‑140 (MQ=255)
      aCTGCCGGCGCTTTTCCAGTATGTATATAACGATTTTATGAAAAACGGCCTGCGTGCCTTTTTGTTTTGTCTGGATTTTGGGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGAc                                                                                                                       <  6:431904/138‑1 (MQ=255)
        tGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGTATTGTGAAAAAATACGCGACAg                                                                                                                     >  3:360888/1‑138 (MQ=255)
        tGTCGGCGCTTTTCCAGTAGGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATGTGGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAg                                                                                                                     <  4:473350/138‑1 (MQ=255)
        ggTCGGCGCTTTTCCAGTTTGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAg                                                                                                                     <  2:651399/137‑1 (MQ=255)
              cgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCa                                                                                                               >  3:530013/1‑138 (MQ=255)
                gttttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCAAGCATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAt                                                                                                             <  6:733176/137‑1 (MQ=255)
                cTTTTCCAGTATGTTGCTAATGATTTTATCAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTCCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAAAACGCGACAGCGCGCAAt                                                                                                             <  1:487832/138‑1 (MQ=255)
                 gttcccAGTAGGTTGCTAACTATTTTCTAAAAAACGGCAGGGGGGCGTTTTTGTTTTGTCTTGATTTTGCGATTTTTCCCCAGCATTCAGACGAAAATCGCCCGGGATTTTTGAAAAAATACGCGACAGCGCGCAATa                                                                                                            <  1:1352976/134‑1 (MQ=255)
                      cAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTGTCTGGATTTTTCGCTTTTTGCCCAGCATTAAGACGAAAAACGCCCGGGTATTGTGAAAAAATACGCCACAGCTCGCAATAACGGt                                                                                                       >  2:931994/1‑138 (MQ=255)
                        gTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCATTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCATtctc                                                                                                   >  8:802786/1‑140 (MQ=255)
                         tATTTTGATAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                                                                                  <  7:235972/140‑1 (MQ=255)
                         tATGTTGATAAAGTTTTTATGACAAACGGCCTGCGGGCTGTTTTGTTTTGGCTGGATTTTGCGCTTTTTGCCCAGCATGCAGACGAAAATCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTtct                                                                                                    <  6:834300/138‑1 (MQ=255)
                          tttttGCTAAAGATTTTTTCAAAAACTGCGTGGGGGCGTTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCACCATTCAGACGAAAATCGCCCGGTATTTGTTAAAAAATACGCGACAGCGCGCAATAACCGTtctc                                                                                                   <  5:240951/135‑1 (MQ=255)
                          ttGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                                                                                                   <  4:1351611/137‑1 (MQ=255)
                          ttGTTGCTAAAGATTTAATGAAAAACGCCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGCCGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                                                                                                   <  2:1037429/137‑1 (MQ=255)
                          aTGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                                                                                                   <  1:639846/138‑1 (MQ=255)
                           tGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTAGCGAc                                                                                                >  7:73114/1‑140 (MQ=255)
                               gCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCAt                                                                                            <  7:1181365/140‑1 (MQ=255)
                               gCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATAGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTc                                                                                              >  4:291678/1‑138 (MQ=255)
                               gCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGTTATTGCGGTTTTTGCCCAGGATTACGAGGAAAGTCGCCCGGGTATTGTGAAAAAATACGCGCCAGCGCGCAATAACCGTTCTCGACTc                                                                                              >  4:874046/1‑138 (MQ=255)
                                cTAAAGATTATATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTGATGGTTTTTGGGCTTTTTGCCCCGCATTCAGACGAAAATCGGCCCGGCATAGTGAAAAAATACGCGACAGCGCGCAATAACCGGTCTCGGCTCa                                                                                             >  6:534849/1‑138 (MQ=255)
                                  aaaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCCGCATTCAGCCGAAAATCGCCCGGGAATTGTGAAAAAATAAGCACCCGCGCGCAATAACCGGTGTCGCCTGATa                                                                                           >  2:1230292/1‑138 (MQ=255)
                                  aaaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGATAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa                                                                                           >  2:328208/1‑138 (MQ=255)
                                     tatttattGAAAACCGGCGGGGGGGCGGTTTTGTTTTGTCTGTTTTTTGCGCTTTTTGCCCCGCATTCTGACGAAAATCGCCCGGTAATTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                                                                      <  7:147028/133‑1 (MQ=255)
                                     gATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCCGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACCGCGCGCAATAACCGTTCTCGACTCATaaaa                                                                                        >  2:8158/1‑138 (MQ=255)
                                       ttttATTAAAAACGGCCTGCGGGCTGTTTTGTTTTGTCGGTATTTTGCGCTTTTTGCCCAGCTTACCGCCTAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                                                                      <  6:1093461/138‑1 (MQ=255)
                                       ttttATGAAAACCGGCCTGCGGGCCGTTTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa                                                                                    <  8:700257/140‑1 (MQ=255)
                                       ttttATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGTCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATAAGCGCCCGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                                                                      >  3:720271/1‑138 (MQ=255)
                                          tATTAAAACGGGCCTGGGGGCGGTTTTGTTTTGTCTGGTTTTTGCCCTTTTTGCCCAGCTTTCAGACTAAAATCGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                                                                   <  2:698843/138‑1 (MQ=255)
                                          tATTAAAAATGGCCTGCGGGCCGTTTTGTTTTGTCTGTTTTTTGCGCTTTTTGCCCATCATTCAGACGAAAATCGCCCGTTAATTTTTAAAAAATACGCGACAGCGAGCAATAACCGTTCTCGACTCATAAAAGTGAttc                                                                                 <  8:582582/140‑3 (MQ=255)
                                          tATTAAAAAGGGCTGGCGGGCCGTTTTGTTTTGTCTGTTTTTTGCGCTTTTTGCCAAGCATTCAGACTAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                 <  7:864195/140‑1 (MQ=255)
                                          tATTAAAAACGGCGTGCGGGCGTTTTTGTTTTGTCTGGATTTTGCGCTTTTTCCCCCGCATTCAGACTAAAATCGCCCGGTAATTTTGAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                                                                   <  1:949177/138‑1 (MQ=255)
                                          tATTAAAAACGGCCTGCGGGGCGTTTTGTTTTGTCTGTATTTTGGGCTTTTTGCCCTGAATTCAGACTAAAATCGCCCGGGAATTGTGAAAAATTACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                                                                   <  4:178419/138‑1 (MQ=255)
                                          tATTAAAAACGGCCTGCGGGCCTTTTTGTTTTGTGTGTTTTTTGCGCTTTTTCCCCAGCATTCAGACGAAATTGCCCCGGGATTTGTTAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                 <  8:1098511/140‑1 (MQ=255)
                                          tATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGTATTTTGCTCTTTTTCCCCAGCTTTCAGACTAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                                                                   <  1:1133333/138‑1 (MQ=255)
                                          tATTAAAAACGGCCGGCGGGCCGTTTTGTTTTGTCTGTTTTTTGCGCTTTTTGCCCCGCATCCACACTAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                                                                   <  1:800565/138‑1 (MQ=255)
                                          tATGAAAACCGGCGGGGGGGCGGTTTTGTTGTGTCGGGATTTTGCGTTTTTTGCCCAGCATCCAGACGAAAATCGCCCGGGAATTGTTAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                                                                   <  1:952669/138‑1 (MQ=255)
                                          tATGAAAAACGGCCTGCGGGCGGTTTTGTTTTGGCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                                                                   <  4:1007417/138‑1 (MQ=255)
                                          tATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                                                                   <  6:1013244/138‑1 (MQ=255)
                                          aaTGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTTTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                                                                   <  4:1027081/137‑1 (MQ=255)
                                            tGAAAAACGGCCTGCGGGCCGTTTTGTTTTGGCTGGTTTTTGCGTTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                 <  3:1281925/138‑1 (MQ=255)
                                            tGAAAAACGGCCTGCGGGCCGTGTTGTTTTGGCTGGTTTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGTATTGTGAATAAATACGCGGGAGCGCGCAATAACCGTTCAGGACACAGATAAGTGATGc                                                                                 >  2:853880/1‑138 (MQ=255)
                                               aaaaCGGCCTGGGGGCCGTTTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCATCATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGc                                                                              <  4:935978/138‑1 (MQ=255)
                                                      ccTGCGGGCCGTTTTTTTTTTGCTGGATTTTGGGCTTTTTGCCCAGCATTCCGACGAAAATCGCCGGGGAATTGTGAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATg                                                                       <  5:594641/138‑1 (MQ=255)
                                                      ccTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTCAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATg                                                                       <  2:693367/138‑1 (MQ=255)
                                                       cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                                                                    <  8:1108995/140‑1 (MQ=255)
                                                         gggggCTGTTTTGTTTTGGCTGTTTTTTCCGCTTTTGGCCCCCCATTCAGACGAAATTCGCCCGGGATTTGTGAAAAATTACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                                                                  <  7:48133/138‑1 (MQ=255)
                                                         gggggCCGTTTTTTTTTGTCGGTATTTTCCGCTTTGTGCCCAGAAGTCAGACGAAAATCCCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                                                                    <  5:806038/136‑1 (MQ=255)
                                                           gggCCGTTTTGTTTTGTCTTTATTTTTCGCTTTTTGCCCAGCTTTCAGACTAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                                                                  <  3:703875/138‑1 (MQ=255)
                                                                  tttgttttgtCTGGAATTTGCGCATTTTGCCCCGCATTCAGACGAAAAGAGCCCGGGAATTTTGAAAAAATAAGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAATTGATGCCGCTATAATGCCGCGTCCTAtt                                                           >  4:726589/1‑138 (MQ=255)
                                                                   ttgttttgtCTGGATTTATCGTTTTTTCCACAGCATTCAGACCAAAATCGCCCGTTATTTGTAAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGa                                                        <  7:1029284/140‑1 (MQ=255)
                                                                    tttttggtCTGGATTTTGCTCTTTTTGCCAAGCATTCAGACGAAAATCGCCCGGTAATTGTTAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa                                                       <  7:645361/138‑1 (MQ=255)
                                                                    tgttttgtCTGGATTTTGGGCTTTTTGCCCAGAATTCACACGAAAATCGCCCGGGAATTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTg                                                         <  6:1223114/138‑1 (MQ=255)
                                                                             tGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttta                                                >  3:202266/1‑137 (MQ=255)
                                                                             tGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc                                                >  6:324602/1‑138 (MQ=255)
                                                                             tGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc                                                >  6:1173813/1‑138 (MQ=255)
                                                                             tGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc                                                >  3:1106214/1‑138 (MQ=255)
                                                                             tGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGGCCTATTTGAATGCTTTc                                                >  1:845136/1‑138 (MQ=255)
                                                                               taTTTTGCGCTTTTTGACCAGCATTCAGACGAAAATCGCCCGGGAATTGTCAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAATTGAATGCTTTCGGga                                            <  8:1194153/139‑1 (MQ=255)
                                                                               gATTTTGCGCTTTTTGCCCTGCATTCTGACTAAAATCGCCGGGTAATTGTGAAAATTTCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCgg                                              <  2:1237546/138‑1 (MQ=255)
                                                                                  tttGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgat                                           >  5:1268586/1‑138 (MQ=255)
                                                                                  tttGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGACGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgat                                           >  3:1180633/1‑138 (MQ=255)
                                                                                   ttGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatg                                          <  6:434935/138‑1 (MQ=255)
                                                                                     gcgcTTTTTGCCCCGAATTCCGAATAAACTCGCCCGGTATTTGTCAAAAAAGACGCGACAGCGCGCAATAACCGTTATCGACTCATAAAATTGATGCCGCTATAATGCCGCGTCATATTTGAATGCTTTCGGGATGATTc                                      <  7:240420/140‑1 (MQ=255)
                                                                                         tttttGCCCAGCATTCAGAGTAAAATCGCCCGGGAATTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGt                                  <  8:821043/140‑1 (MQ=255)
                                                                                         tttttGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTg                                    <  4:670244/138‑1 (MQ=255)
                                                                                         tttttGCCCACCATTCAGACTAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTg                                    <  5:533719/138‑1 (MQ=255)
                                                                                         tttttGCCAACCATTCAGACGAAAATCGCCCGGGAATTGAGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTg                                    <  1:406870/138‑1 (MQ=255)
                                                                                         tttttCCCCAGCATTCCAACTAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTg                                    <  5:36729/138‑1 (MQ=255)
                                                                                         tttttCCACTGCATTCGCACTAACTTCGCCGGGTAATTGTCAAAAAATACGCGACAGGGCGCAATTACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATTCTTTCGGGATGATTCTg                                    <  5:29960/138‑1 (MQ=255)
                                                                                             tGCCCAGTTTTCTGATTAAAATCCGCGGGTAATTTTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTaa                                <  1:821392/138‑1 (MQ=255)
                                                                                             tGCCCAGCATTCAGACGAAATGCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTaa                                <  3:1100160/138‑1 (MQ=255)
                                                                                             tGCCCAGAATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTaa                                <  2:1005214/138‑1 (MQ=255)
                                                                                             ggCCAAGCATTCAGACGAAAATCGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTaa                                <  3:985982/137‑1 (MQ=255)
                                                                                               cccAGAATTCAGACCAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACa                              <  2:914468/138‑1 (MQ=255)
                                                                                                 cAGAATTCAGACGAAAATCGCCCGTGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAgg                            <  6:1026904/138‑1 (MQ=255)
                                                                                                   ccATTCCGACGAAAATCGCCCGGGAATTGGTAAAAAATACGCGACAGCGCGAAATAACCGTTCTCGACTCATAAAAGTGATGCAGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGa                          <  2:1285925/137‑1 (MQ=255)
                                                                                                       tCATACTAAAATCGCCCGGTAATTGTGAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAAtgt                      <  2:452313/138‑1 (MQ=255)
                                                                                                       tCAGCCGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtga                    <  7:1050336/140‑1 (MQ=255)
                                                                                                       tCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAAtgt                      <  3:960434/138‑1 (MQ=255)
                                                                                                       tCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGGTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTTGTACCAGGGAAtgtgt                    >  7:833733/1‑139 (MQ=255)
                                                                                                       gcacaCGAAAATCGCCCGGGAATTGTGAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAAtgt                      <  3:67541/134‑1 (MQ=255)
                                                                                                        cAGACGAAAATCCCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAAtgtg                     <  5:1018461/138‑1 (MQ=255)
                                                                                                         aGACGAAAATCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtga                    <  3:966054/138‑1 (MQ=255)
                                                                                                           aCGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtgatt                  >  3:702805/1‑138 (MQ=255)
                                                                                                               aaaTCGCCCTGTAATTGTGAAAAAATACGCGACAGCGCGAAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTgattgatt              <  6:235746/138‑1 (MQ=255)
                                                                                                                aaTCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATta             >  5:289750/1‑138 (MQ=255)
                                                                                                                  acGCCCGGGAATTGTTAAAAACTACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCAGAGTCCTATTTGAATGCTTTCGGGATGATTCTTTTAACAGGGAATGTGATTGATtata           <  2:1018110/137‑1 (MQ=255)
                                                                                                                    gCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGaa       <  8:450236/140‑1 (MQ=255)
                                                                                                                    gCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAg         <  2:1136902/138‑1 (MQ=255)
                                                                                                                    gCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAg         <  6:1320762/138‑1 (MQ=255)
                                                                                                                    gCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAg         <  5:1091836/138‑1 (MQ=255)
                                                                                                                    gCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGaa       <  8:205923/140‑1 (MQ=255)
                                                                                                                         gtaaTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATTAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACAt    <  2:1229192/136‑1 (MQ=255)
                                                                                                                         ggAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGATGGCTTTCGGGATGATTATGGTAACAGGGAATGTGATTGATTATAATACCAt    >  6:1143187/1‑138 (MQ=255)
                                                                                                                         ggAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACAt    >  6:1143188/1‑138 (MQ=255)
                                                                                                                           aaTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGACCATcc  >  1:376446/1‑138 (MQ=255)
                                                                                                                           aaTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATcc  >  1:1286076/1‑138 (MQ=255)
                                                                                                                                 |                                                                                                                                   
TTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCC  >  NZ_CP022686/4014162‑4014422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A