Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F35 I0 R1	271	8871.0	10354886	98.4%	10189207	136.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	4,014,291	G→T	21.6%	intergenic (+137/‑207)	bolA → / → tig	transcriptional regulator BolA/trigger factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	4,014,291	0	G	T	21.6%	207.9 / 11.7	167	intergenic (+137/‑207)	bolA/tig	transcriptional regulator BolA/trigger factor
Reads supporting (aligned to +/- strand): ref base G (46/70); new base T (3/40); total (49/118)
Fisher's exact test for biased strand distribution p-value = 3.39e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

AAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCC  >  NZ_CP022686/4014155‑4014429

aaaCTCATTTGCACCTGTCGGCGCTTTTCCATTATGTTGCTAAATATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTGaaa                                                                                                                                         <  4:74552/140‑1 (MQ=255)

aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGTTTTTGCGAGTTTTGGCCAGGATTCATACGAAAATAGCCCGGGTATTTTTaaa                                                                                                                                         >  6:1272835/1‑140 (MQ=255)

    gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCAGTTTTGTTTTGTCTGGATGTTGCGCATTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTTAAAAAAt                                                                                                                                     >  5:77195/1‑140 (MQ=255)

             aCTGTCGGCGCTTTTCCAGTATGTGGCTAAAGATTTTATGAAAACC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCCTTCAGACGAAAATCGCCCGGGAATTGGGAAAAAATACGCGACAg                                                                                                                            <  8:1127632/140‑1 (MQ=255)

              gtGTCGGCGCTTTTCCAGTATGTTGATAAAGATTTTATTAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAgc                                                                                                                           <  5:1008125/139‑1 (MQ=255)

               tttCGGCTTTTTTCAATTTTTTTTCTAAAGATTTTATAAAAAAG‑GGAGTGGGGGTTTTTTTTTTTTGTCTGTATTTTGGTGTTTTTCCAAAGAATTCAGCCTAAAATCGCGCGGGTATTTTTAAAAAATACGCGACAgcg                                                                                                                          <  6:1198959/138‑1 (MQ=255)

               tctCGGCGCATTACGAGTATGATGATAAAGAATTTATGAAAAAC‑GGCGTGCGGGCGGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCTTTCAGCCGAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAgcg                                                                                                                          <  4:1284085/138‑1 (MQ=255)

               tGTCGGCGCTTTTCCAGTATTTTTCTAAAGATTTAATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAgcg                                                                                                                          <  2:1053049/140‑1 (MQ=255)

                gTCGGCGCTTTTCCAGTATGTTGCTTAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTGCGCTTTTTGACCAGCATTCAGACGAAAATCGCACGGGAATTGTGAAAATATACGCGACAgcgc                                                                                                                         >  4:738363/1‑140 (MQ=255)

                gTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGTTTTTTCGCTTTTTGCCCCGCATTCAGACGAAAATAGCCCGGGAATTGTGAAAAAATAAGCGACCgcgc                                                                                                                         >  5:186998/1‑140 (MQ=255)

                gTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGAAGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAgcgc                                                                                                                         >  6:969172/1‑140 (MQ=255)

                gTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGGCTGGATTTTGAGCTTTTTGCCCAGCATTCAGCCGAAAATAGCCCGGGAATTGTGAAAAAATACGCGACAgcgc                                                                                                                         >  1:786049/1‑140 (MQ=255)

                gTCGGCGCGTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGAccacgc                                                                                                                         >  2:813454/1‑135 (MQ=255)

                     cgcTTTTCCCGTTTGTTGATAAATATTTTATTAAACAC‑GGCCGGCGGGCGGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACTAAACTCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAt                                                                                                                    <  7:1193421/140‑1 (MQ=255)

                       cTTTTCATGTATGTTGCTAAAGATTTTATTAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa                                                                                                                  <  2:95323/140‑1 (MQ=255)

                          ttCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTAAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAccc                                                                                                               >  4:529708/1‑139 (MQ=255)

                              tttttGTTGCAAAAGATTTTATGAAAAAC‑GGCCTGCGGGCTGTTTTGTTTTTTCTGTATTTTGGGCTTTTTGCCCAGCATTCAGACGAAAATCGCCGGGTAATTGTTAAAATATACGCGACAGCGCGCAATAACCGTtct                                                                                                           <  6:723604/136‑1 (MQ=255)

                                tttGTTCCTAAATATTTTATCAAAACG‑GGCGGGCGGGCCGTTTTGTTTTGTCTGGTTTTTGCGTTTTTTGCCCACAATACAGACTAAAATCGCCCGGTAATTGTCAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                                                                                         <  3:436066/138‑1 (MQ=255)

                                tATTTTGATAAATATTTTTTTAAAAAC‑GGCCTGCGGGCGTTTTTGTTTTGTTTGGATTTTGCGCTTTTTGCCCAGAATTCAGACTAAAATCGCCCGGTATTTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                                                                                         <  1:316587/140‑1 (MQ=255)

                                 ttGTTGATAATTATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGAATTCAGACGAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                                                                        <  3:231109/139‑1 (MQ=255)

                                 aTGTTGTAAAAGATTTAATTAAAAAC‑GGCCTGCGGGCCTTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGAATTCAGACTAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                                                                        <  1:541003/140‑1 (MQ=255)

                                  tGTTGCTAAAGATTTAATGAAAACC‑GGCCGGCGGGCGGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGAATTCCGACGAAAAGCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAc                                                                                                       <  2:1167288/140‑1 (MQ=255)

                                    ttGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCGTTTTTCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGCCAGCGCGCAATAACCGGTCTCGACTc                                                                                                     >  6:564667/1‑140 (MQ=255)

                                     tGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATAGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTAGACTCa                                                                                                    >  3:199312/1‑140 (MQ=255)

                                      gCTAAAGATTTAATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTTTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCTGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCAt                                                                                                   <  8:1149390/140‑1 (MQ=255)

                                       ttataGATTTTATTAATCAC‑GGCGTGCGGGCCGTTTTGTTTGGTCGTGATTTTGCGCTTTTTGCCCAGAATTCAGTCGTAAATCGCCCGTGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa                                                                                                  <  2:532273/136‑1 (MQ=255)

                                       cTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa                                                                                                  >  2:357605/1‑140 (MQ=255)

                                       cTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCTTTTTGACCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAAAAAGCGACAGCGCGCAATAACCGTTCTCGACTCaaa                                                                                                  >  4:370664/1‑138 (MQ=255)

                                                 tataAAAACC‑GGCGTGGGGGCGTTTTTGTTTTGTCTGTATTTTGCGTTTTTTCCCCAGAATTCAGACTAAAATCGCCCGGTAATTGTCAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                        <  1:154330/140‑1 (MQ=255)

                                                 tATTAAAATC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGTTTTTTGGGCTTTTAGCCCTCGATTCAGACGAAAATCGCCGGGGAATTCTCAAAAAAAACGCGACAGCGCGCAATATCCGTTCTCGACTCATAAAAGTGATGc                                                                                        <  3:215773/140‑1 (MQ=255)

                                                 tATTAAAACC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGTAATTGTGAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                        <  7:455532/140‑1 (MQ=255)

                                                 tATTAAAACC‑GGCCTGCGGGCCGTTTTGTTTTGGCTGTATTTTGCGCTTTTTCCCCAGCATTCAGACTAAAATCGCCCGGTATTTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                        <  3:560541/140‑1 (MQ=255)

                                                 tATTAAAAAC‑GGCTTTCGGGCCGTTTTGTTTTGTCTTGATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGTAATTGTTAAAAAATACGCGCCAGCGCGCAATAACCGTTCTCGACTCATAAAAGGGATGc                                                                                        <  8:1007346/140‑1 (MQ=255)

                                                 tATTAAAAAC‑GGCCTGCGGGACGTTTTGTTTTGTCTGTATTTTGCGCTTTTTGCCAAGCATTCATACGAAAATCGCCCGGGAATTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                        <  3:761132/140‑1 (MQ=255)

                                                 tATGAAAACC‑GGCGGGGGGGCGGTTTTGTTTTGGCTGGATTTTGCGCTTTTTGCCCAGCTTTCATCCGAAAATCGCCCGGGATTTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                        <  5:618085/140‑1 (MQ=255)

                                                 tATGAAAAAC‑GGCCTTCGGGCGGTTTTGTTTTGTCTGGAATTTGCGCTTTTTCCCCATCATTCAGACGAAAATCCCCTGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                        <  6:942842/140‑1 (MQ=255)

                                                 tATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTGTGGTTTTTTCGCGTTTTGCCCAGCATTCAGACGGAAATCGCCCGGGAATTGTGAAAAAATACGCGACCGCGCGCAATAACCGTTCTAGCCGCATAAAAGTGATGc                                                                                        >  1:1046218/1‑140 (MQ=255)

                                                 tATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                        <  5:239652/140‑1 (MQ=255)

                                                 tATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                        >  4:250572/1‑140 (MQ=255)

                                                 tATGAAAAAC‑GGCCTGCGGGCCGTTTGGTTTTGTCTGTATTTTGCGGTTTTTGCCCAGAATTCAGACGAAAATCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                        <  4:100375/140‑1 (MQ=255)

                                                 tATGAAAAAC‑GGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCCGCATTCAGACGAAAATCGCCCGGGTATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                        >  1:1022937/1‑140 (MQ=255)

                                                 tATGAAAAAC‑GGCCTGCGGGCCGTGTTGTTTTGGGTGGAGTTTGCGCGTTTTGGCCCGGATTGAGACGAAAAGCGCCCGGGAATTGTGAAAAAATAAGCGGCCGCGCGCCATAACCGGTCGGGCCTCATTAAAGTGATgg                                                                                        >  3:376374/1‑139 (MQ=255)

                                                 tATGAAAAAC‑GGCCTGCGGGCCGTGTTGGTTTGTCTGGATTTTGCGGGTTTTGGCCCGCCTTCAGCCGCAAATCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCCAAAACCGTTCTCGCGGCATTAAAGTGATGc                                                                                        >  5:937029/1‑140 (MQ=255)

                                                 tATGAAAAAC‑GGCCGGGGGTCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGAATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                        <  6:281417/140‑1 (MQ=255)

                                                 tATCAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                                                                        <  3:632820/140‑1 (MQ=255)

                                                 attGAAAAAC‑GGCGTGCGGGCGGTTTGTTTTTGTCTGGATTTTGCGCTTTTGGCCCCTCATTCATACTAAAATCGCCGGTTAATTTTGAAAAAAAACGCGACAGCGCGCAATAACCGGTCTCGACTCATATAAGTGATGc                                                                                        <  6:1210294/138‑1 (MQ=255)

                                                 actcaaaaaC‑GGCCTGCGGCACGTTTTGTTTTGTCTGGATTTTGCGCTTTTAGCCCCCCAATCTCCCCAAAATCGCCCGGCATTTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTCATGc                                                                                        <  6:731327/136‑1 (MQ=255)

                                                   tGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGTTTTTGCGCTTTTTGCCCCGCATTCACACTAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCg                                                                                      <  4:21451/140‑1 (MQ=255)

                                                    ttaaaaCGGGCCTGCGGTCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCAAGCATTCAGACGAAAATCGCCCGGTAATTGTGAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCg                                                                                      <  7:958079/138‑1 (MQ=255)

                                                               gtGCGGGCGGTTTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGTAATTGTAAAAAAAACCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                                                                           <  1:646530/139‑1 (MQ=255)

                                                               cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                                                                           <  6:613262/140‑1 (MQ=255)

                                                               cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                                                                           <  2:1293263/140‑1 (MQ=255)

                                                               cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGATTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                                                                           <  1:325975/140‑1 (MQ=255)

                                                               cTGCGGGCCGTTTTGTTTTGTCGGGATTTTGCGCTTTTTGCCCAGCATTCAGACTAAACTCGCCCGGTAATTGTTAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcg                                                                           <  7:548704/140‑1 (MQ=255)

                                                                 gggggCGGTTTTTTTTTGTTTGTTTTTTCGGTTTTTTGCCCAGCATTCTGACTAAAATCGCCCGGTATTTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                                                                         <  1:471628/138‑1 (MQ=255)

                                                                 gcgggcggTTTTGTTTTGTCTTGATTTTGCGCTTTTGGCCCAGCATTCAGACTAAAAGCGCCGGGTAATTGTTAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                                                                         <  5:1247634/140‑1 (MQ=255)

                                                                 gcgggcggTTTTGTTTTGTCTGGATTTGGCGCTTTTTGCCCAGAATTCAGACTAAAATCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                                                                         <  7:1068663/140‑1 (MQ=255)

                                                                 gCGGGCCGTTTTTTTTTGTTTGGATTTTGCGCTTTTTGCCCAGCATTCACACGAAAACCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                                                                         <  1:272750/140‑1 (MQ=255)

                                                                 gCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCACCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgcg                                                                         <  4:315516/140‑1 (MQ=255)

                                                                   gggCCGTTTTGTTTTGTCTGGATTTTGGTCTTTTTGCCACGAATTCAGACTAAAATCTCCCGGGAATTGTGAAAAACTACGCGACAGCGCGCAATAACCGGTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTc                                                                       <  4:36072/140‑1 (MQ=255)

                                                                   gggCCGTTTTGTTTTGTCTGGATTTTGCGTTTTTTGCCAAGCATTCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTc                                                                       <  1:511255/140‑1 (MQ=255)

                                                                            tgttttttCTGTTTTTTGCTCTTTTTCCCCACAATTAATACTAAAATCGCCCGGGATTTTTCAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa                                                              <  7:274493/140‑1 (MQ=255)

                                                                            tgttttgtGTGTATTTGGCGCTTTTTGCACATCATTATGACGAAAATCCCCTGGTATTTGTTAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATATTGCAGCGTCCTATTTGaa                                                              <  5:509707/140‑1 (MQ=255)

                                                                            tgttttgtGTGTATTTGGCGCTTTTTCCCCATCATTCACACGAAAAGCGCCCGGTAATTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa                                                              <  6:270283/140‑1 (MQ=255)

                                                                            tgttttgtCTGGATTTTGACCTTTTTGCCCAGCATTCAGACTAAAATCGCCCGGGATTTGTTAAAAACAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa                                                              <  7:91100/140‑1 (MQ=255)

                                                                                 tgtCTGGATTTTGCGCTTTTTGCCCTGATTTCAGACGAAAATCGCCCGTTAATTTTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCtt                                                         <  8:63167/140‑1 (MQ=255)

                                                                                  gtCTGGATTTTGCGCTTTTTGCCCATAATTCAGACTAAAATCGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt                                                        <  3:1270735/140‑1 (MQ=255)

                                                                                   tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttta                                                       >  7:379550/1‑139 (MQ=255)

                                                                                   tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttgc                                                       >  1:629357/1‑138 (MQ=255)

                                                                                   tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc                                                       >  2:217653/1‑140 (MQ=255)

                                                                                   tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc                                                       >  6:820676/1‑140 (MQ=255)

                                                                                   tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTc                                                       >  3:1226544/1‑140 (MQ=255)

                                                                                       taTTTTGCGCTTTTTGCCCAGCATTCCGACGAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga                                                   <  2:912492/139‑1 (MQ=255)

                                                                                       gATTTTGCTCTTTTTGCCCAGCATCCAGCCGAAAATCGCCCGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga                                                   <  7:663824/140‑1 (MQ=255)

                                                                                           ttGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatgat                                               <  8:368248/140‑1 (MQ=255)

                                                                                            tGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGAtt                                              >  6:1058050/1‑140 (MQ=255)

                                                                                             gcgcTTTTTTCCAAGCATTCAGACGAAAATCGCCGGGGATTTGTCAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTc                                             <  4:595304/140‑1 (MQ=255)

                                                                                             gcgcTTGTTCCCCATCATTCATACGAAAAGCGCAGGGTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTc                                             <  2:147394/140‑1 (MQ=255)

                                                                                                 tttttGCCCAGCATTCATACGAAAATCGCCCGGGAATTGTGAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGt                                         <  2:1022937/140‑1 (MQ=255)

                                                                                                  ttttGCCCAGCATTCCCACGAAACGCGCCCGGGAATTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTa                                        <  5:351252/140‑1 (MQ=255)

                                                                                                     tGCCCCGCATTCAGACTAAAATCGCCCGGGATTTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACa                                     <  1:41634/140‑1 (MQ=255)

                                                                                                       ccaagaaTTCATACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAgg                                   <  2:287002/134‑1 (MQ=255)

                                                                                                         cAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGa                                 <  4:414496/140‑1 (MQ=255)

                                                                                                           gCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAAt                               >  3:677105/1‑140 (MQ=255)

                                                                                                               tgaGACTAAATGCGCACGGTAATTGTGAATAAATACGCGACTGCTCGGATTATCCGTTCTCGACTCATATAAGTGATGCCGCTATTAGGCCGCGTCCTATTAGATTGCTTGGGGGATGTGTCTGGTAACAGGGAATGtga                           <  8:892252/138‑1 (MQ=255)

                                                                                                               taaGACTAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtga                           <  7:1071814/138‑1 (MQ=255)

                                                                                                               tCAGACTAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtga                           <  2:217893/140‑1 (MQ=255)

                                                                                                               tCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCCGGTAACAGGGAATGtga                           <  3:1125846/140‑1 (MQ=255)

                                                                                                               tCAGACGAAAATCGCCCGGCAATGTTGAAAAAATACGCGACAGAGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCGGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtga                           <  2:412136/140‑1 (MQ=255)

                                                                                                               tCAGAAGAAAATCGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtga                           <  1:556861/140‑1 (MQ=255)

                                                                                                                 aGACTAAAATCGCCCGTTAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtgatt                         <  4:469119/140‑1 (MQ=255)

                                                                                                                 aGACGAAAATCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCTCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGtgatt                         <  8:611882/140‑1 (MQ=255)

                                                                                                                      aaaaTCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATta                    >  8:828685/1‑140 (MQ=255)

                                                                                                                      aaaaTCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATta                    >  7:100757/1‑140 (MQ=255)

                                                                                                                       aaaTCGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATtat                   <  1:183410/140‑1 (MQ=255)

                                                                                                                             cccGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAAc             <  7:723828/140‑1 (MQ=255)

                                                                                                                             cccGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAAc             >  1:1125819/1‑140 (MQ=255)

                                                                                                                                 ggAATTGTGAAAAAATACGCGACAGCGCGCAATAAACGTTCTCGACTCATAAAAGTTATGCCGCTATAATTACTCGTCCTAATTGAATGCTTTCGGTATCAATCTGGTAACAGGTAAAGTGATAGATTATAAGAAAATcc         >  7:805107/1‑140 (MQ=255)

                                                                                                                                    aTTGTGAAAAAATCCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCgg      <  5:515671/140‑1 (MQ=255)

                                                                                                                                        tgAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCACGGTTcc  >  1:987706/1‑140 (MQ=255)

AAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAC‑GGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATCGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCC  >  NZ_CP022686/4014155‑4014429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A