Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F12 I0 R1 158 5737.7 7407908 98.3% 7281973 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,905,170 T→G 14.2% V99G (GTT→GGT)  CIW80_RS24815 → coenzyme F390 synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,905,1700TG14.2% 182.7 / 2.5 110V99G (GTT→GGT) CIW80_RS24815coenzyme F390 synthetase
Reads supporting (aligned to +/- strand):  ref base T (40/50);  new base G (17/2);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 3.00e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CCCTTTAATCAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGA‑CGGTTTATTTGCCAAAGAG  >  NZ_CP022686/4905031‑4905306
                                                                                                                                           |                                                                                                                                             
cccTTTAATCAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGGTTAAAACCCTGCGt                                                                                                                                               >  2:309120/1‑140 (MQ=255)
cccTTTAATCAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCGGCGt                                                                                                                                               >  6:386440/1‑140 (MQ=255)
    ttAATCAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCTACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGAATTAAAACCCAGCGGTggg                                                                                                                                           >  2:863458/1‑139 (MQ=255)
    ttAATCAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGc                                                                                                                                           <  6:122689/140‑1 (MQ=255)
    ttAATCAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGc                                                                                                                                           <  3:357845/140‑1 (MQ=255)
    ttAATCAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGc                                                                                                                                           <  3:3201/140‑1 (MQ=255)
    ttAATCAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGc                                                                                                                                           <  1:570309/140‑1 (MQ=255)
    ttAATCAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAAGGCGCAGTGAGCAGAGTCGTGAGTTCAAACCCTGCGTTGGc                                                                                                                                           <  4:73188/140‑1 (MQ=255)
      aaTCAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAg                                                                                                                                         <  4:493729/140‑1 (MQ=255)
         ctgTGGCCTTTGTTGGATAAAGCGTTAATGATGACGCACTGCGATGAGATGAATTCCGCAGGTTTAAAGCGCGATTAACTTCTCGATTGTGCAATGGGCAGTGAGCAGAGTCGTGATTTCATACCCTGCGGTGGCAGGtt                                                                                                                                      <  6:638123/138‑1 (MQ=255)
         cAGTTGCCAATGATGGATACATCGTTAATTATGACGCACTTCGATGATATTAATAACGGAGTTTTAAATCTCGACGATCTTCTCTATTGTGGAATGCGCATTGAGCAGAGTCGTGATTTCAAAACCGGCGTTGGCAGGtt                                                                                                                                      <  2:618246/140‑1 (MQ=255)
         cAGTGTACAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGtt                                                                                                                                      <  4:637161/140‑1 (MQ=255)
         cAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGtt                                                                                                                                      <  2:648190/140‑1 (MQ=255)
          aGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTc                                                                                                                                     >  4:634479/1‑140 (MQ=255)
          aGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCTGCGGTGGCAGGttg                                                                                                                                     >  4:23308/1‑139 (MQ=255)
           gTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAACGCGCGACGACCTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTCAAACCCAGCGTTGGCCGGttga                                                                                                                                    >  4:875900/1‑138 (MQ=255)
           gTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCa                                                                                                                                    <  2:448510/140‑1 (MQ=255)
           gTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACATCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAAACCACCGGTGGCAGGGTCa                                                                                                                                    >  4:899464/1‑140 (MQ=255)
            tGGCCAATGATGGATAAAGCTTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCTTGAATTCAAACCCAGAGTTGGCAGGTTCAg                                                                                                                                   >  2:913880/1‑140 (MQ=255)
            tGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTAAAAACCAGCGTTTGAAGGttg                                                                                                                                     >  7:3475/1‑137 (MQ=255)
            tGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCAGCGTTGGGAGGGTCAg                                                                                                                                   >  2:371852/1‑140 (MQ=255)
            tGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCTGCGTTGGCAGGTTCAg                                                                                                                                   >  1:457660/1‑140 (MQ=255)
            tGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAAATTCTCGATTGTGCAATGCGCAGTGAGAAGAGTCGTGATTTAAAAACCCGCGTTGGCAgggtaag                                                                                                                                   >  2:550813/1‑135 (MQ=255)
              gCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTCAAACCCTGGAGTGGCCGGTTGAg                                                                                                                                   >  7:108260/1‑138 (MQ=255)
              gCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCGGCGGTGGGCGGGTCAg                                                                                                                                   >  8:591941/1‑138 (MQ=255)
                cAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAgtg                                                                                                                                 <  8:208100/138‑1 (MQ=255)
                   tgatgGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCCGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGGGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTggg                                                                                                                            <  4:225441/140‑1 (MQ=255)
                   tgatgGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGCATTGAAACACTGCGTTGGCAGGGTCAggggtg                                                                                                                              >  8:667919/1‑133 (MQ=255)
                      tgGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGc                                                                                                                           <  7:822140/138‑1 (MQ=255)
                      tgGATAAAGCGTTAATGATGACGCACTTCGAGGAGATGAATTCCGCAGGTTTAAAGCGCGACGAACTTCTCGATTTTGCAAAGCGCAGGGAGCAGTGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCtt                                                                                                                         <  6:117517/140‑1 (MQ=255)
                          tAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAATCGCGACGAACTTCTCGATTGTGCAATGCGCAGAGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtc                                                                                                                       <  7:276492/138‑1 (MQ=255)
                          tAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtcat                                                                                                                     <  1:165365/140‑1 (MQ=255)
                          tAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtcat                                                                                                                     <  1:698118/140‑1 (MQ=255)
                           aaaGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtcatc                                                                                                                    <  6:642897/140‑1 (MQ=255)
                            aaGCGTTAATGATGCCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTtcat                                                                                                                     <  8:209553/138‑1 (MQ=255)
                             aGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGTTTTAAAACCCAGCGTGGGGAGGGTTAGTGTTGGGGTTtcaagat                                                                                                                  >  3:392633/1‑136 (MQ=255)
                             aGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTCAAAACCAGCGGTGGGAGGGTGAGTGTTTGGGTTTGATGAg                                                                                                                  >  1:41077/1‑140 (MQ=255)
                             aGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCAGCGTTTGGAGGGTAAGTGTTTGGCGTTCATCAg                                                                                                                  >  6:912342/1‑140 (MQ=255)
                                  tAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACa                                                                                                            <  1:528900/140‑1 (MQ=255)
                                  tAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAAACCTGCGTTTGCACGTTGAGTTTTGGGCTTTCATAAG‑GTaaa                                                                                                            >  3:661553/1‑138 (MQ=255)
                                   aaTGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGCTTTAAAACCCCGGCGGGGGTGGTTCAGTGTTGGGCGTTCATAAG‑Gtcaaa                                                                                                           >  5:114360/1‑136 (MQ=255)
                                       atgaCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCggg                                                                                                       <  4:233100/140‑1 (MQ=255)
                                         gaCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGTTTTCAAACCCAGCGTTGGCGGGTTCAGGGGTTGGCGTTGATGAG‑GTTCAtacgggc                                                                                                     >  6:592374/1‑134 (MQ=255)
                                         gaCGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGAGTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTAAAAACCAGGGTTGGGAGGGTCAGTGTTGGGGTTTACTAAG‑GTTCATAGGgtg                                                                                                     >  5:840170/1‑139 (MQ=255)
                                           cGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACATCTCGATTGTGCAATCCGCAGTGAGCAGAGTCGTGATATCAAACCCAGCCTTGGGACGTTAAGTGTTGGGCATTCATCAC‑GAACAAAGGgtg                                                                                                     >  7:658255/1‑137 (MQ=255)
                                             cACTTCGATGAGATGAATACCGCAGTTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGtcgt‑c‑g                                                                                               <  6:712779/140‑1 (MQ=255)
                                             cACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGtcgt‑c‑g                                                                                               <  4:912971/140‑1 (MQ=255)
                                             cACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGtcgg‑c‑g                                                                                               <  6:444076/140‑1 (MQ=255)
                                             cACTTCGAGGAGATGAATACCGCAGGTTTAAAGCGCGACGCACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCGTTCATCAG‑GTACATCGGGtcgt‑c‑g                                                                                               <  6:334805/140‑1 (MQ=255)
                                              acgtCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGGGCAATGCGCAGTGAGCAGCGGCGTGCTGTCAAAAACTGGGGGGGGAGGTTCAGTGGTGGGCTGTCATAAG‑GTTCTTCGCggcgtggg                                                                                                >  4:650573/4‑137 (MQ=255)
                                                               aCCGCAGGGTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGGCGT‑C‑G‑TGGGCTTTTTGTTGTTAg                                                                            <  6:788372/140‑1 (MQ=255)
                                                                   cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTAAAACCCAGCCGTGGCAGGTTGAGTGTTGGGCTTTGATCAG‑TTTCATCGGGTCGG‑CGG‑TGGGGTTTTTTTTTTTAGccc                                                                         >  5:117979/1‑140 (MQ=255)
                                                                   cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTTAAACCCAGCGGTGGCACGGTCAGTGTTGGGCTGTCATCAC‑GTTAAAGGGGGCGT‑C‑G‑TGGGCTttttttttttttccac                                                                        >  5:535214/1‑134 (MQ=255)
                                                                   cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCTGCGTTGGGAGGTTCAGTGTTGGGGTTTCATCAG‑GTACATAGGGGCGT‑G‑G‑TGGGCGTTTTTTTGTTAGcccc                                                                        >  4:816793/1‑139 (MQ=255)
                                                                   cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACACAGCGTTGGCCGGTTCAGTGTTGGGCTTTGATCAG‑GGTCATAGGGGCGT‑C‑G‑GGGGGGTTTTTTTTTTTGcccc                                                                        >  4:151743/1‑139 (MQ=255)
                                                                   cAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAAACCTGGGGTGGGCGGTTAAGTGTTTTGCGTTGATCAG‑GTACAACGGGTCGT‑C‑G‑GGGGCTTTTTtttgttttcc                                                                          >  8:554966/1‑134 (MQ=255)
                                                                   cAGGTTTAAAGCGAGACGAAATTCTCGATTGCGCAATGCGCAGTGAGCAGAGTCGTGAGTTCAAACCCAGCGTTGGCAGGGTGATGGTTTGGCTTTAAAAAT‑GTTCATACGGTCGG‑C‑G‑TGGGCTTTTTTTTGTTAgac                                                                          >  8:273725/1‑136 (MQ=255)
                                                                     ggTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTAAAACCCAGGGTTTGGAGGTTCAGTGTTGGGGGTTCCTCAG‑GTAAATAGGGTCGT‑G‑G‑TGGGGGTTTTTTTGTTTGcccc                                                                        >  8:595417/1‑137 (MQ=255)
                                                                      gTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGTTTTCAAACCCAGCGTTGGGAGGTTCCGTGTTGGGGTTTAATAAG‑GTACATCGGGGGGT‑C‑G‑TGGGGTTTTTTTTTTTATCCCGCAc                                                                     >  4:761963/1‑140 (MQ=255)
                                                                      gTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCAGCGTTGGGAGGGTGAATGGTGGGCTTTGATCAG‑GTTCATCGGGTGGT‑G‑G‑TGGGGGTTTTTTTGTTTGACCGccc                                                                     >  4:783607/1‑138 (MQ=255)
                                                                       tttAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACg                                                                    <  1:603882/140‑1 (MQ=255)
                                                                         tAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAg                                                                  <  3:23308/140‑1 (MQ=255)
                                                                         tAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAg                                                                  <  3:634479/140‑1 (MQ=255)
                                                                         tAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCAGCCTGGGGAGGGTCAGGTTTGTGGGTTCATCAA‑GTGCATCGGGTGGT‑C‑G‑TGGGGTTTTTTTTGTTAGgcccccccag                                                                  >  3:57205/1‑133 (MQ=255)
                                                                          aaaGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGc                                                                 <  4:673984/140‑1 (MQ=255)
                                                                            cgcgacacgaACTTCTCTATTGATCAATGAGCAGTGCGCAGAGTAGTGTTTTCATACCCTGCGTTTTCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCaa                                                               <  1:315711/134‑1 (MQ=255)
                                                                            aGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTCCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCaa                                                               <  2:138114/140‑1 (MQ=255)
                                                                            aGCGCGACGAACTTCGCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCaa                                                               <  2:953615/140‑1 (MQ=255)
                                                                                cgacgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCATCGTTGGCACGTTCAGTGTTGGGCTTTCATCCG‑GTACCTCGGGTCGT‑A‑G‑TGGGGGTTTTTTTGTTTGCCCGCCCGAGCAACAGa                                                           >  4:545759/1‑140 (MQ=255)
                                                                                  ccgaACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGAtg                                                         <  3:678206/139‑1 (MQ=255)
                                                                                  acgaCCTTCTCGATTGTGCAATGCGCAGGGAGCCGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGGCGT‑C‑G‑TGGGCTTTTTTTTGTTAGCCCGCACGAGCAACAGa                                                           <  8:711/138‑1 (MQ=255)
                                                                                       cttcttGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAAGCCTGCGTGGGCAGGTTCCGTTTTGTGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCAGCACGAGCAACAGATGTgg                                                      <  7:178582/138‑1 (MQ=255)
                                                                                       cttcttGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTgg                                                      <  7:178580/138‑1 (MQ=255)
                                                                                        ttCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTAAAACCCAGGGTTGTCAGGTTGAGTGTTGGTGTTTAATCAG‑GTACATCGGGTCGT‑G‑G‑TGGGGTTTTTTTTGTTTTCCCGCACGAGCAACAGATTTggggc                                                   >  1:516287/1‑138 (MQ=255)
                                                                                          ctcGATTGTGCAATGCGCAGTGAGCAGAGTCGTGGTTTAAAACACAGCGATGGGACGGTACGGGTTGGGCTTTAATCAGCGCAC‑TAGGGTCGT‑C‑G‑TGGGGGTTTTTTTTTTATCCCCCCAGACCCACAGATGTGGgcccc                                                 >  6:572219/1‑138 (MQ=255)
                                                                                            cGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGgccgc                                                 <  8:708266/138‑1 (MQ=255)
                                                                                            cGATTGGGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGGTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGccgccg                                               <  6:374156/140‑1 (MQ=255)
                                                                                               ttGTGCAATGCGCAGTGAGCAGAGTCGTGATATAAAACCCAGCGGAGGCAAGGTAAGAGTTTGGCGTTTATAAG‑GTAAAAATGGTGCT‑G‑A‑TGGGCATTTTTTTTTTAGCCCCCACCAACAACAAAAGATGGCCCCCGgtg                                            >  4:630872/1‑140 (MQ=255)
                                                                                                    cAATGCGCAGTGAGCAGAGTCGTGGTTTCAAACCCAGGGGTGGCCGGGTCAGGGTTGGGGTTTTATCAG‑GTTCATAGGGTCGG‑A‑G‑TGGGGGTTTTTTTGTTATACCCCCCCAGCCACAACAGTTGGCCGCCCGtgtgg                                         >  8:629914/1‑137 (MQ=255)
                                                                                                        gcgcAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCaa                                   <  5:260628/140‑1 (MQ=255)
                                                                                                            aGTGAGCAGAGTCGTGCTTTCAAAACCTGCGTTGTCAGGTTCAGTGTTGGGCTTTCATAAGTGTA‑ATAGGGTCGT‑C‑G‑TGGGAGTTTTTTTTTTAGACCGCACCTGCAAAATATTTTGGCCGCCGGTGTGGTGTCcaaaat                               >  4:546850/1‑138 (MQ=255)
                                                                                                                 gCAGAGTCGTGATTTCAAACCCTGCGTTGGGAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGGGTTTTTTTTTTTGCCCGCCCCCGCAAAAGCTGTGGGGCGCCGGGGTGCTGGCAAAAGTTTTgg                          >  6:668593/1‑139 (MQ=255)
                                                                                                                  cAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGcc                         <  4:910770/140‑1 (MQ=255)
                                                                                                                   agagTCGTGATTTCAAACCCAGGGGTGGGTGGTTGATTGGTGGGGGTTCATCAC‑GGGCGTCGGGGCGTGG‑G‑TGGGGTTTTTG‑TGTTATCGCGCGCGCTCAACACATTTGGGCCGCCCGTGTGCTGGCCAAAATGTTGccc                        >  6:54700/1‑140 (MQ=255)
                                                                                                                     agTCGTGATTTAAAACACGGCGTTGGCAGGTTCAGTGTTGGGCGTTAATAAG‑GTAAAAAGGGTAGT‑A‑GTTGGGGTTTTTTTTTTTTGCCC‑CCCACACAACAGAAGTGGGCCGCCCGTGTGGTGGGCAAAGTGTTTCCCgg                      >  2:555417/1‑139 (MQ=255)
                                                                                                                       tCGTGTTTTAAAACCCTGCGTTGGGAGGTTAAGTGTTGGGGTTTGATGAG‑GTACATAGGGTCTT‑C‑G‑TGGGCTTTTTGTTGTTATCCCGCACGATCCACACATGTGGGGCGCCGGGGTGCGGGCCAAACTGTTGCCCgc                      >  8:236307/1‑137 (MQ=255)
                                                                                                                           gATTTAAAACACTGCGTTGGCAGTTTCAGAGTTGGGCTGTAATCAG‑GTACATCGTGTCGT‑C‑G‑TGGGCTTTTTGTTGTAAGCCAGCAAGAGCAACAGATGTGGGCCTCCGGTGTGCTGGCAAAAGTGTTGCCCTA‑CGGttt               <  6:908554/140‑1 (MQ=255)
                                                                                                                            aTTTAAAACCCAGCGTTGGCAGGTTCAGTGTTGGGCTTTGATAAG‑GTACAAAGGGTCGT‑C‑G‑TGGGGTTTTTTTTGTTTTCCCCCAACAGCAAAACATTTGGGCCCCCGGTGTGCTGGCAAAAATGTTTCCCCACCGGttt               >  2:184677/1‑140 (MQ=255)
                                                                                                                               taaaaCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGG‑C‑G‑TGGGGATTTTGTTGTTAGCCCGCCCGGGCAACAGATGTGGGCCGCCGGTGTGCTGGCCAAAGTGTTGCCCGC‑CGGGtttttt           >  4:679901/3‑140 (MQ=255)
                                                                                                                                cAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCGTTCATCAG‑GTACATCGGGGCGG‑C‑G‑GGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGGGTGCTGGCAAAAGTGTTGCCCGA‑CGGTTTAtt            <  8:281583/138‑1 (MQ=255)
                                                                                                                                 aaaCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTTTTGTTAGCCCGCACGCGCAACAGAAGTGGGCCCCCGGTTGGCTGGCAAAAGTGTTGCCCGA‑CGGTGTATTTgg         >  3:699541/1‑139 (MQ=255)
                                                                                                                                 aaaCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCGTTTTGTTGTTAGGCCGCCCGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGC‑CGGGTTTTTTgg         >  2:268669/1‑139 (MQ=255)
                                                                                                                                  aaCCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATAAG‑GGACATCGGGTCGT‑A‑G‑TGGGCTTTTTTTTTTTAGCCCGCACGCGCAACAGATGTGGGCCCCCGTTGTGCGTGCAAAATGGTTTGCCCA‑CCGGTTATTTTcc        >  2:202423/1‑140 (MQ=255)
                                                                                                                                  aaCCCTGCGTTGGCAGGTTAAGTGTTGGGATTTCATCAG‑GTACAAAGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTTTCCCGACAGCGCAACAAATGTGGGACGCCGGTCTTCTGGCAAAAGTGTTTCCCGA‑CCGGTTATTTGcc        >  2:872699/1‑140 (MQ=255)
                                                                                                                                       tGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTTTTTTTTGCCCGCCCGAGCCACAGCTGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGA‑CGGTTTATTTGCCAAagc   >  5:840497/1‑139 (MQ=255)
                                                                                                                                       tGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGA‑CGGTTTATTTGCCAAaga   >  4:36264/1‑140 (MQ=255)
                                                                                                                                        gCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGC‑CGGTTTATTTGCCAAagag  >  3:295300/1‑140 (MQ=255)
                                                                                                                                         cGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCCACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGC‑CGGTTTATTTTCCAAaga   >  7:755722/1‑138 (MQ=255)
                                                                                                                                          gTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTgttgtt                                                                              <  7:807108/63‑1 (MQ=255)
                                                                                                                                          gTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTgttgtt                                                                              >  8:807108/1‑63 (MQ=255)
                                                                                                                                           |                                                                                                                                             
CCCTTTAATCAGTGGCCAATGATGGATAAAGCGTTAATGATGACGCACTTCGATGAGATGAATACCGCAGGTTTAAAGCGCGACGAACTTCTCGATTGTGCAATGCGCAGTGAGCAGAGTCGTGATTTCAAACCCTGCGTTGGCAGGTTCAGTGTTGGGCTTTCATCAG‑GTACATCGGGTCGT‑C‑G‑TGGGCTTTTTGTTGTTAGCCCGCACGAGCAACAGATGTGGGCCGCCGGTGTGCTGGCAAAAGTGTTGCCCGA‑CGGTTTATTTGCCAAAGAG  >  NZ_CP022686/4905031‑4905306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A