Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F2 I0 R1 252 7301.5 9636800 98.5% 9492248 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 5,001,164 A→C 13.7% N163T (AAC→ACC)  ompG → monomeric porin OmpG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226865,001,1640AC13.7% 312.5 / 5.4 189N163T (AAC→ACC) ompGmonomeric porin OmpG
Reads supporting (aligned to +/- strand):  ref base A (86/70);  new base C (4/28);  total (91/98)
Fisher's exact test for biased strand distribution p-value = 9.24e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

TATCACTACGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCAATAACGGTGAGT  >  NZ_CP022686/5001025‑5001301
                                                                                                                                             |                                                                                                                                         
attCACCACGTTTATGAACGGGTAAAATCCACGGTTAATATGGGGCGCTGGAAAATTGCCCCAGACTGGTTTGTT‑AAATTGACTTCCT‑ATTTAGGTTACAACGGTTGGCTGTCGATTTAAAAATTTGAAAACGATTTTaa                                                                                                                                           <  8:144623/138‑1 (MQ=255)
 aTCACTACGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGAATGTGATGTG‑AAACTGACTGACG‑ATTAACGTTACAACGGATGACTGTCGATGTATAAATTTGCCAACGATTTGAac                                                                                                                                          >  5:534736/1‑140 (MQ=255)
   cACTACGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAacac                                                                                                                                        >  5:152159/1‑140 (MQ=255)
   cACTACGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAacac                                                                                                                                        >  6:404850/1‑140 (MQ=255)
   cACTACGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAac                                                                                                                                          >  1:1157055/1‑138 (MQ=255)
   cACTACGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAac                                                                                                                                          >  1:863159/1‑138 (MQ=255)
    aCTAGGTTTATGACCCGGTTAACGACAGGGCTAATATGCAGCGCGTTAAAATTGCGCATGACTGGTA‑GTGTAAACTGACTGAAG‑ATTTACTTTAAAACGGTGGGCTGTCGATGTATAAATTTGCCAAGTATTTCAACACg                                                                                                                                       <  6:217786/140‑1 (MQ=255)
    aCTACGTTGTTGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACg                                                                                                                                       >  8:637015/1‑140 (MQ=255)
      tACGTTGATGAACCGGGTAAAGACACGGCTATTATGCAGCGCTGTAAAATTGCGCCAGACTGGTATGTG‑AAACTGACTGACG‑ATTTACGTTTCAAGGGTTGGCTGTCGATTTATAAATTTGCCAACGATTTGAACACg                                                                                                                                       <  2:87460/138‑1 (MQ=255)
      tACGTTGATCAACCGGTTAAAGACCCGGCTAATTTCCAGCGCTGTAAAATTGCGCCAGACTGGGATGTT‑AAACTGACTGACG‑ATTTACGTTTCAACGGTGGGCTGCCGATGTATAAATTTGCCAACTATTTGAACACg                                                                                                                                       <  2:1208729/138‑1 (MQ=255)
        cGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCg                                                                                                                                   >  7:873287/1‑140 (MQ=255)
          ttGATGAACCGGTTAAAGACACGGCTAATATGCAGCGCTGTAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCACCGGTTGGCTGTCGATTTATAAATTTGCCAACGATTTGAACACGACCGGt                                                                                                                                 <  6:1177984/140‑1 (MQ=255)
             atgaACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGGCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTAc                                                                                                                              >  7:97419/1‑140 (MQ=255)
             atgaACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTAc                                                                                                                              >  3:1091100/1‑140 (MQ=255)
             atgaACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTAc                                                                                                                              >  8:423482/1‑140 (MQ=255)
             atgaACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGT‑‑AAACTGACTGACG‑GTTTAAGGTTAAACGGTTGGCTGTCGATGTATAAATTTTCCAAAGAATTGCACACGACCGGTTAcc                                                                                                                             >  5:179165/1‑139 (MQ=255)
                  ccGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCt                                                                                                                           >  1:777837/1‑138 (MQ=255)
                  ccGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTC‑AAACTGACTGACG‑ATTAACGTTACAACGGTTGGCTGTCGATGTATAACTTTGCCAACGATTTGAACACGACCGGTTACGCTGa                                                                                                                         >  4:530035/1‑140 (MQ=255)
                     ggTAAAGACACGGCGAATATGCAGCGCTGTAAAATTGCGCCAGACTGGTATGTG‑AAACTGACT‑ACGTATTTACGTTTCACAGGTTGCCTGACGATGTATAAATTTGCCAACCATTTGAAAACGACCGGTTACGATGATAc                                                                                                                      >  6:856407/1‑140 (MQ=255)
                     ggTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATAc                                                                                                                      >  5:557547/1‑140 (MQ=255)
                     ggTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATAc                                                                                                                      >  8:1100224/1‑140 (MQ=255)
                           cacagGGCTAATATGCAGCGCGGTAAATTTGCGCCAGACTGGGATGTT‑AAACTGAATGACG‑ATTTACTTTTCAAGGGTTGGCTGTCTATGTATAAATTTGCCATCGATTTGAACACGACCGGTTACGCTGATACCCgt                                                                                                                  <  2:1139794/137‑1 (MQ=255)
                               cGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCACCGGTTGGCTGTCGATGTATAAATTTGCCAACGCTTAGAACACGACCGGTTACGCTGATACCCGTGTCgaa                                                                                                            >  6:707339/1‑140 (MQ=255)
                               cGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACCCGACCGGTTACGCTGATACCCGTGTCgaa                                                                                                            >  4:1172003/1‑140 (MQ=255)
                                ggCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑ATACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGACCACGACCGGTTACGCTGATACCCGTGTCgaaa                                                                                                           >  6:525462/1‑140 (MQ=255)
                                ggCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGACCACGACCGGTTACGCTGATACCCGGGTCgaaa                                                                                                           >  8:131605/1‑140 (MQ=255)
                                ggCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCgaaa                                                                                                           >  5:986925/1‑140 (MQ=255)
                                ggCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTCAACACGACCGGTTACGCTGATACCCGTGTCgaaa                                                                                                           >  7:421019/1‑140 (MQ=255)
                                 gCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCACCGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGATCACGACCGGTTACGCTGATACCCGTGTCgaa                                                                                                            >  2:181761/1‑138 (MQ=255)
                                 gCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCgaa                                                                                                            >  2:243747/1‑138 (MQ=255)
                                  cGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCACCGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCgaaa                                                                                                           >  1:1240640/1‑138 (MQ=255)
                                      tatGCAGCTCTGGAAAATTGCGCCAGACTGGTATGTG‑AAACTTACTGACT‑ATTTACGTTTCACCGGTTGGATGTCGATGTATAAATTTGCCAACGATTTTAACACGACCGGTTACGCTGATACCCGTGTCgaaacggaaa                                                                                                     <  6:59484/140‑1 (MQ=255)
                                      tatGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGATGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCgaaacggaaa                                                                                                     >  3:611366/1‑140 (MQ=255)
                                         ccAGCGGTGTAATATTGACGCAGACTGGGATTTG‑AAACTGAGTGCCT‑ATTTACGTATCAAGGGATGGCTGTCGTTTAAAAAATTTGCCAACGATTTTAACACGACCGGTTAAGCTGATACCCGTGTCGaaacggaaacgg                                                                                                  <  4:557390/139‑1 (MQ=255)
                                           ggcgcTGGAAAATTGCGCCAGAGTGGGATGTG‑AAACTGACTGAGG‑ATTTAGGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGt                                                                                                <  4:1045549/139‑1 (MQ=255)
                                           cgcgcTGCAAAATGGCGCAAGACTGGGATGTC‑AAACGGACTCCCG‑ATTTCCGTTTCAACGGTGGGCTGGCGAGGAAAAAATTTGCAAACGATTTGCCCACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGt                                                                                                <  8:519506/139‑1 (MQ=255)
                                           cccccTGCAAAATTGCGCCAGACTGTGATTTT‑AAACTGAATGACT‑ATTTAGGTTGCAACGGTTGGCTGTCTATGTATAAATTTGCCAAATATTTGAACACGACCGGTTACGCTGATACCCGTGTCGaaacggaaacgg                                                                                                  <  1:708158/134‑1 (MQ=255)
                                           aGCGATGGAAAATTGCGCCAGACTTGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTAGGCAGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTAACTCTTATACACGTGTCTAAACGGAAACgggg                                                                                                >  6:907697/1‑139 (MQ=255)
                                            gcgcGGTAAATTTGCGCCAGACTGGGATGTT‑AAACTGCATGACT‑ATTTACGTTTCACCGGGTGGCTGTCGATGTATAAATTTGCCAACGATTTTAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACggg                                                                                                 <  2:1072606/138‑1 (MQ=255)
                                             cgcTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTca                                                                                              >  5:965359/1‑139 (MQ=255)
                                              gcTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGACCACGACCGGTTCCGCTGATACCCGTGTCGCAACGGAAACggggc                                                                                               >  1:1101357/1‑136 (MQ=255)
                                              ccTGCAAAATTCCCCCAGACGGGTATGTC‑AACCTCACTGCCT‑ATTACCGTTACAAGGGTCGGCTGTCTATTTAAAAATTTCCCACCTATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTc                                                                                               <  2:624440/137‑1 (MQ=255)
                                                       gtGCGCAAGCCTGGGATGTG‑AACCGCTCTGACG‑ATTTCCTTTTAACCGGTTGGCTGTCGCTGTATAAATTTCCCCACGATTTTAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATAcc                                                                                    <  4:95284/139‑1 (MQ=255)
                                                         gcgcCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATAcc                                                                                    >  2:987089/1‑138 (MQ=255)
                                                         ccgcCAGATTGGTATGTC‑AAACTGAGTGACG‑ATTTACTTTACAACGTTTGGCTGTCTATTTAAAAATTTCCAAACGATTTCAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCtt                                                                                  <  3:915048/139‑1 (MQ=255)
                                                            ccAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCaa                                                                               >  7:1043424/1‑140 (MQ=255)
                                                               taCTGGGATGTG‑AAACTGACTGTGT‑ATTTAGGTTTCAAGGGTTGGTTGTGTATGTTTAAATTTGCCAACGTTTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGa                                                                            <  6:965359/139‑1 (MQ=255)
                                                               caCTGGGATGTG‑AAACTTACGGACT‑ATTTACGTTTCAACGGTGGGTTGTCGATGTATAATTTTGCCAACTATTTGACCACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGa                                                                            <  4:137178/139‑1 (MQ=255)
                                                                     tttgtt‑AAACTGACTGACT‑TTTTCCGTTTAATCGGTTGGCTGTCTCTTTATAAATTTCCCAAGGTTTTCACCACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGt                                                                      <  6:264386/138‑1 (MQ=255)
                                                                     gATGTG‑AACAGGAGTGCCG‑ATTAAGGTTTCAAGGGTGGTCTGGCTATTTATAATTTTGCCTACTTTTTTAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACg                                                                        <  2:269584/138‑1 (MQ=255)
                                                                         tg‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGCCCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGcc                                                                  >  5:911165/1‑140 (MQ=255)
                                                                         tg‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGcc                                                                  >  3:300671/1‑140 (MQ=255)
                                                                         tg‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGcc                                                                  >  7:230411/1‑140 (MQ=255)
                                                                           taaaCTGAGTTACT‑ATTTACGTTTCAACGGTTGGCTGTGGATTTATAAATTTGCCCACTATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCt                                                                 <  4:897139/139‑1 (MQ=255)
                                                                           taaaCTGAATGACT‑ATTTAGTTTTCAACGGTTGTCTGTCGATGTATAAATTTGCCAACGATTTGAACAAGACCGTTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCt                                                                 <  4:706901/139‑1 (MQ=255)
                                                                           caaaCTGACTGACG‑AATTCCGTTACACGGGTTGGGGGTCTATTTAAAAATTTCCCACCTATTCGACCACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGc                                                                   <  1:477363/137‑1 (MQ=255)
                                                                            aaaCTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGcc                                                                  >  1:94569/1‑138 (MQ=255)
                                                                              actgactgACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCtt                                                                >  1:406316/1‑138 (MQ=255)
                                                                               ctgactgaCG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTGCAACGAAACGGTTGCCTTGCg                                                             >  5:876857/1‑140 (MQ=255)
                                                                                 gacttacG‑ATTTACTTTTCAACGGTGGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCg                                                             <  2:777837/138‑1 (MQ=255)
                                                                                   ctgacT‑ATTTACGTTTCAACGTTTGGCTGTCTATTAATAAATTTGCCAACTATTTGAACACGACCGGTTACGCTGATACCAGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAg                                                           <  1:848632/138‑1 (MQ=255)
                                                                                     gacG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACTATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGaa                                                       <  4:44629/140‑1 (MQ=255)
                                                                                      ccG‑ATTTACGTTTCAACGGTTGGCGGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGGCGAAACGGAACCGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGa                                                        >  2:72547/2‑138 (MQ=255)
                                                                                        g‑ATTTACGTTTCAACGGTTGGATGTCGATGTATAAATTTGCAAACGATTTGAACAAGCCCGGTTACGCTGATAACAGTGTCGAAACGGAATCGGGTCTGAAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAAc                                                      >  2:375473/1‑138 (MQ=255)
                                                                                          aTTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAAAGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGCTCTGCAATATACCTTCACCGAAACGGTTGCCTTGCGAGTGAACtat                                                   >  8:213904/1‑140 (MQ=255)
                                                                                           tttACTTTTCAAGTGTTGGGTGTCGATTTATAAATTTGCCAAGTATTTTAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACta                                                    <  2:15060/138‑1 (MQ=255)
                                                                                           tttACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACtatt                                                  >  8:939555/1‑140 (MQ=255)
                                                                                             tACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAACCGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACtatt                                                  >  1:242070/1‑138 (MQ=255)
                                                                                             tACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCAAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAACCGGTTGCCTTGCGAGTGAACtattat                                                >  5:975751/1‑140 (MQ=255)
                                                                                                     aagggTTGGCTTTCTATGTTTAATTTTGCCAAGGATTTCAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAg                                          <  2:94569/135‑1 (MQ=255)
                                                                                                     aagggTTGGCTGTCTTTGTTTAAATTTGCCACCTATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAgcg                                        <  4:736900/137‑1 (MQ=255)
                                                                                                     aaCGGTTGGCTTGCGTTGAAAAAATTTCCCAACTTTTTCACCACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAgcg                                        <  3:242907/140‑1 (MQ=255)
                                                                                                     aaCGGTTGGCTGTGTATGTATAAATTTGCCAACTATTTCAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAgcg                                        <  3:544380/140‑1 (MQ=255)
                                                                                                     aaCGGTTGGCTGTCGATTTATAAATTTGCCAACGATTTCACCACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAgcg                                        <  5:404850/140‑1 (MQ=255)
                                                                                                     aaCGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGACCACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAg                                          <  2:112037/138‑1 (MQ=255)
                                                                                                     aaCGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAgcg                                        >  6:1116045/1‑140 (MQ=255)
                                                                                                         ggtgccTGTCTATTAATAATTTTGCCAACGATTTGACCACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGt                                    <  8:60939/135‑1 (MQ=255)
                                                                                                         gTTGGCTGTCGATGTAAAAAGTTGCCAACGATATGACCACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGcgcg                                      <  2:1027924/138‑1 (MQ=255)
                                                                                                         gTTGGATGTCGATGTATCAATTTCCCAAGGATTTGAACACGACCGGGTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCCACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGCGCGCGGt                                    <  8:78191/140‑1 (MQ=255)
                                                                                                           tGGCTTGCGAGGTATAAAATTGCCAACGATTGCAACACGACCGGGTACGCTGAGACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGttt                                  <  8:834999/140‑1 (MQ=255)
                                                                                                           gggCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGttt                                  <  4:609801/139‑1 (MQ=255)
                                                                                                            ggCTGTAGATGTATAAATTTGCCAACGATTTGAACACGACCGGTGACGCTGATACCCGTCTCTAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCATTGCGAGTGAACTATTATCTCGAGCGCGGTTTc                                 >  7:574938/1‑140 (MQ=255)
                                                                                                               tGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAat                              <  4:168458/140‑1 (MQ=255)
                                                                                                                gtctttGTTTAATTTTGCCAACTTTTTGACCACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAata                             <  7:195376/135‑1 (MQ=255)
                                                                                                                   gATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAatat                            >  1:1055005/1‑138 (MQ=255)
                                                                                                                           attttGCCAAGGTTTTTACCACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGc                  <  4:403352/138‑1 (MQ=255)
                                                                                                                           aaTTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGc                  <  6:116231/140‑1 (MQ=255)
                                                                                                                           aaTTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGc                  >  4:496974/1‑140 (MQ=255)
                                                                                                                           aaTTTGCAAACTATTTTACCACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGc                  <  4:544499/140‑1 (MQ=255)
                                                                                                                               tGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCtt                                                                                  <  5:111793/72‑1 (MQ=255)
                                                                                                                               tGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGgat                        >  3:356713/1‑130 (MQ=255)
                                                                                                                                gCCAACTATTGAAACACGACCGGTTACGCTGATACCCGTGTAGAAACGGAATCGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGATGATAGCCGCaa             <  8:587048/140‑1 (MQ=255)
                                                                                                                                 ccAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCAAt            >  5:784520/1‑140 (MQ=255)
                                                                                                                                 ccAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCAAt            >  3:844052/1‑140 (MQ=255)
                                                                                                                                 ccAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACATTCAACTAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCAAt            >  6:520010/1‑140 (MQ=255)
                                                                                                                                   aaCGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATa                    <  2:39855/130‑1 (MQ=255)
                                                                                                                                   aaCGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATa                    >  1:39855/1‑130 (MQ=255)
                                                                                                                                   aaCGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACATTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCAAt            >  1:573725/1‑138 (MQ=255)
                                                                                                                                       aTTTGAATTCGAGCGGTTACGCTGATACGCGTGTCGAAAGGGCTACGTGTCTGCAATATAGCTTCATCGTAAAGGTTGCCATGCGAGTGAACTTTTATCTCGAGAGCGGTTTTAATATGGATGATAGCCGCAATAACGGt      <  8:748203/140‑1 (MQ=255)
                                                                                                                                         ttGAACACGACCGGTTACGCTGATACCCGTGGCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCAATAACGGt      <  1:1070122/138‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                         
TATCACTACGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTG‑AAACTGACTGACG‑ATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAACACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCAATAACGGTGAGT  >  NZ_CP022686/5001025‑5001301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A