Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F13 I0 R1 235 6960.4 9135780 98.4% 8989607 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 5,001,164 A→C 21.8% N163T (AAC→ACC)  ompG → monomeric porin OmpG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226865,001,1640AC21.8% 189.6 / 17.4 134N163T (AAC→ACC) ompGmonomeric porin OmpG
Reads supporting (aligned to +/- strand):  ref base A (63/39);  new base C (2/30);  total (65/69)
Fisher's exact test for biased strand distribution p-value = 9.61e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

ATCACTACGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCAAT  >  NZ_CP022686/5001026‑5001291
                                                                                                                                          |                                                                                                                                
aTCACTACGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAa‑c                                                                                                                                >  6:792049/1‑140 (MQ=255)
  cACTACGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAa‑c                                                                                                                                >  4:446990/1‑138 (MQ=255)
   aCTACGTTGATGAACGGGGTAAATACACGGCTAATATGCAGCGCTGTAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAAGGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACg                                                                                                                             <  8:898853/140‑1 (MQ=255)
     tACGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGAc                                                                                                                           >  1:788927/1‑140 (MQ=255)
       cGTTGATGAACCGGGTAAATACACGGCGAATATGCAGCGCTTGAAAATTGCTCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGGCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCg                                                                                                                         >  5:199278/1‑140 (MQ=255)
       cGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCATACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCg                                                                                                                         >  5:36756/1‑140 (MQ=255)
        gTTGATGAACCGGGTAAACACAGGGCTAATATGCAGCGCTGTAAATTTGCGCCAGACTGGGATGTTAAACTGACTGACGATTACCGTTTCAACGGTGGGCTGTCGATGTATAAATTTGCCAACTATTTGAC‑CACGAcc                                                                                                                          <  4:1150896/138‑1 (MQ=255)
         ttGATGAACCGGGTAAAGACACGGCTAATATGCAGCGCTGGAAAATTGCGCCAGACTGGTATGTCAAACTGACTGACGATTTACGTTTCAACGGTGGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGt                                                                                                                       <  8:537946/140‑1 (MQ=255)
          tgatgaACGGGGAACCGACCCGGCTAATATGCAGCGCTGGAAAATCGCGCCAGAGTGGGATGTGAAACTGACTGACGATTTACGTTTCAAGGGTGGGCGGTCGATGTAAAAATTTCCCAACGATTTGAA‑CACGACCGGtt                                                                                                                      <  2:842666/140‑1 (MQ=255)
            atgaACCGGGTAAAGACACGGCGAATATGCATCTCTGGAAAATTGAGACAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCACCGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTAc                                                                                                                    >  2:546084/1‑140 (MQ=255)
            atgaACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTAc                                                                                                                    >  5:559053/1‑140 (MQ=255)
            atgaACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTAc                                                                                                                    >  5:553416/1‑140 (MQ=255)
              gaACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTAc                                                                                                                    >  3:31504/1‑138 (MQ=255)
               aaCCGGGTAAAGCCACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGCTTTGAA‑CACGACCGGTTACGCt                                                                                                                 >  7:361280/1‑140 (MQ=255)
                 cggggAAAAGCCAGGTCTAATAGTCAGCGATGTAAATTTGCGCCAGTCTGCTATGTGAAACTGACTGACTATTTACTTTTCAACGGTGGGCTGTCTTGGTATAAATTTGCAAACTATTTGAC‑CACGACCGGTTACGCTGa                                                                                                               <  2:98182/138‑1 (MQ=255)
                 ccGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGa                                                                                                               >  5:188171/1‑140 (MQ=255)
                 ccGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGa                                                                                                               >  5:1075643/1‑140 (MQ=255)
                    ggTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATAc                                                                                                            >  1:839929/1‑140 (MQ=255)
                    ggTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATAc                                                                                                            >  7:172131/1‑140 (MQ=255)
                    ggTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATAc                                                                                                            >  7:172128/1‑140 (MQ=255)
                               ggCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAACCTGACTGCAGATTTAAGTTTCACCGGCTGGCTGGCGATGTATAATTTTGCCAACGATTTGCA‑CACGACGCGTTACGCTGATTCCAGTGTCgaaa                                                                                                 >  5:36627/1‑140 (MQ=255)
                               ggCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGAAGTGAAACTGACTGACGATTTACGATTCAACGGTTGGCTGTCGATCTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTCATACCCGAGTCgaaa                                                                                                 >  1:1166031/1‑140 (MQ=255)
                               ggCGAAAATGCAGCGCTGGAAAAATGCGCCAGACAGGGATGTGAAAATGACTGACGATTTAAGTTTCAACGGTAGGCTGTCGATGAATAAATTTGCCAACTATTTGAA‑CACGACCGGTTACGCAGATACACGTGTCgaaa                                                                                                 >  8:465081/1‑140 (MQ=255)
                                gCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCgaa                                                                                                  >  3:155411/1‑138 (MQ=255)
                                gCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCgaa                                                                                                  >  3:750818/1‑138 (MQ=255)
                                 cGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACTCTGATACCCGTGTCgaaa                                                                                                 >  3:820641/1‑138 (MQ=255)
                                 cGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCgaaa                                                                                                 >  3:937644/1‑138 (MQ=255)
                                 cGAATATGCAGAGCAGGAAAATTGCGCAAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCACCGGTTGGCTGTCGATGTATAAATTTGCCAACGAATTGAA‑CCCGACCGGATACGCTGCTACCCGTTTCgaaa                                                                                                 >  4:767558/1‑138 (MQ=255)
                                     tatGCCGCGCCGCAAAACTGCGCCCGCCTGGTTGGTGAACATCACTGACGTTTACCGTTGCTCCGGTTGGGTGTGTATTTATAAATTTCCCACGTATTTGAC‑CACGACCGGTTACGCTGATACCCGTGTCgaaacggaaa                                                                                           <  2:345137/140‑1 (MQ=255)
                                         cAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACtgg                                                                                       >  6:244555/1‑140 (MQ=255)
                                         cAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACggg                                                                                       >  7:582022/1‑140 (MQ=255)
                                          ctcgcTGTAAAATCGCCCAACACGGGGTTGTCAAAGTGACTAACGATTAACTTGACATGGGTGGGCGGCCTAGGTAAAATTTTCCCAACTATTACAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGt                                                                                      <  5:604668/138‑1 (MQ=255)
                                          cgcgcTTGAAAATTGCCACCTACTGGTTTGTTAAACGCCCTGACTATTTAGGTTGAAAGGTTTGGCTGGCTATTTATAAATTTCCCAACGATTGGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGt                                                                                      <  7:742620/139‑1 (MQ=255)
                                          cgcgcTGTAAAATTGCGCCTGACTGGTATGTGAAACTGACTGACTATTTCCGTTGCACCGGTTGGCTGTCTATGTATAAATTTCCCAACGTTTTGAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGt                                                                                      <  6:1059257/139‑1 (MQ=255)
                                          cgcgcTGGAAAATTGCGCCAGCCGGGTATTGGAAACTGACTGACTATTTACGTTGCAACGGTTGGCGGGCGAGGTATAAATTTGCCACCGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGaaacggaaacgg                                                                                        <  4:402399/137‑1 (MQ=255)
                                          aGCGCTGTAAAATTGCGCCAGACTGGGATGTGAAACTGACTGCCGATTTACGTTTCAACGGTTGGCTGTCTATGTATAAATTTGCCACCGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGaaacggaaacgg                                                                                        <  4:1103508/138‑1 (MQ=255)
                                          aGCGCTGGAAAATTGCGCCTGCCTGGGAGGTGAAACTGACTGACGATTTACGTTTCAACGGGTGGCTGGCGATGTATAAATTTGCCAACTATTTGAC‑CACGACCGGTTACGCTGATACCCGTGTCGaaacggaaacgg                                                                                        <  4:1024342/138‑1 (MQ=255)
                                          aGCGCTGGAAAATTGCGCCAGAGGGGGATGTGAAACTGACTTACGATTTACGTTTCAAGGGTTGGCTGTCGTTGTATAAATTTCCCAACTATTTTAC‑CACGACCGGTTACGCTGATACCCGTGTCGaaacggaaacgg                                                                                        <  3:791430/138‑1 (MQ=255)
                                           gcgcTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACggg                                                                                       >  4:406284/1‑138 (MQ=255)
                                             gcTGGAAACTTGCGCCAGACGGGGTTGTCAACCTGCCTCACTATTTCCTATTCACGGGTTGGCTGCGTATGAATAAATTTCCAAACGATTTGAC‑CACGACCGGTTACGCTGATCCCCGTGTCGAAACGGAAACGGGTCTg                                                                                   <  6:327099/140‑1 (MQ=255)
                                                 taaaaTTCCCCCAGACTGGTTTGTGAAACTGCCTGACTATTTACGTTTCAACGGTTGGCTGTCTATGTAAAAATTTGCCAAGGATTTGAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAat                                                                               <  2:14069/139‑1 (MQ=255)
                                                   aaaTTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGATTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTAACGCTGATACCCGGGTCGAAACGGAAACGGGTCTGCAat                                                                               >  4:1135853/1‑138 (MQ=255)
                                                      ttGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATAcc                                                                          >  6:596066/1‑140 (MQ=255)
                                                      ttGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATAcc                                                                          >  2:716636/1‑140 (MQ=255)
                                                        gcgcCTGAGGGGGTTGTGAAACTGACTGAGTTTTTAAGTTTCAAGGGTTGGTTGTCTATTTATAAATTTGCAAACTTTTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCtt                                                                        <  8:418933/140‑1 (MQ=255)
                                                        gcgcCAGATTGGGATGTTAAAATGACTGAGTATTTAGTTTTCAAGGGTTGGCTGTCTATTTATAATTTTCCCAACTATTTCAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCtt                                                                        <  1:401731/140‑1 (MQ=255)
                                                        gcgcCAGACTGGGATGTTAAACTGACTGACTATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCtt                                                                        <  5:448661/140‑1 (MQ=255)
                                                             ctcatGGTATGTTAAACTTACTGACTATTTACGTTACACTGGTTGGTTTAATATGTATAAATTTGCCAAAGATTTGAC‑CACGACCTGTTAAGATGATAACCGTGTCGAAACGGAAACTGGTCTGCAATATAACTTCaa                                                                     <  4:807236/134‑1 (MQ=255)
                                                                gtGGGATGGTAAACTGACTGACGATTTACGTTGCAACGGTGGGCTGTCGATTAATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTGTGCAATATACCTTCAACGaaa                                                                <  2:624309/139‑1 (MQ=255)
                                                                    tttgctaaaCTGACTCCCTATTTCCGTTACACGGGTTGGGTGTCTATTAATAAATTTCCAAAAGATTACAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGt                                                            <  1:364766/134‑1 (MQ=255)
                                                                       gagAACCTGACTGACTATTTAGGTTACAACTGTTGGCTGTCTATTTATAAATTTGCCAACGATTTCAA‑CACGACCGTTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGc                                                         <  6:44949/138‑1 (MQ=255)
                                                                          aaaCTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCACCGAAACGGTTGcc                                                        >  3:90649/1‑138 (MQ=255)
                                                                           aaCTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTg                                                     <  6:972693/140‑1 (MQ=255)
                                                                              ggactgacCATTTACGTTTCAACGGTGGGCTGTCTATGTAAAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGa                                                  <  2:522748/139‑1 (MQ=255)
                                                                                actgacGATTTACGTTTCAACCGATGGCTGTAGATGTATAAATTTGCCAACGATATGAA‑CACTACCGGTTACGCTGATACCCCTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAAAGAAACGGTTGCCTTGCGAGt                                                >  5:986282/1‑140 (MQ=255)
                                                                                 ctgattaTTTACTTTTCACCGGTTGGGTGTCTTTTAAAAAATTTGCCAACTTTTTCAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTg                                               <  7:233156/134‑1 (MQ=255)
                                                                                 ctgacTTTTTACTTTTCACGGGTGGGCTGGCGATTTAAAATTTTCCCACCGTTTTCAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTg                                               <  7:758958/140‑1 (MQ=255)
                                                                                 ctgacTATTTACGTTTCACCGGTTGGCTGTCGATGTATAAATTTCCCAAATATTTTAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAg                                                 <  3:948248/138‑1 (MQ=255)
                                                                                 ctgacTATTTACGTTTCAAGTGTGGGCTGTCGATGTATAATTTTCCCAACTATTTCAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTg                                               <  6:196984/140‑1 (MQ=255)
                                                                                 ctgacGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTg                                               <  8:246824/140‑1 (MQ=255)
                                                                                 ctgacGATTTACGTTTCAACGGTTGGCTGTCGAGGTAAAAATTTGCCAACTATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTg                                               <  8:213917/140‑1 (MQ=255)
                                                                                 ctcagtaTTTACGTTTCACCGGTGGGCTGGCGATTTATAAATTTCCCAACTATTTGAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTg                                               <  7:583540/134‑1 (MQ=255)
                                                                                 cggacTATTTAGGTTTAAAGGTTTGCGTGTCTATTTATAAATTTGCAAAGTTTTTTAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAg                                                 <  3:576542/136‑1 (MQ=255)
                                                                                 attcctaTTTCCTTTGCAACGGTTGGCTGTCCATGTATAAATTTCCAAACGATTTGAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTg                                               <  2:104948/134‑1 (MQ=255)
                                                                                  tgacTATTTACGTTGCCACGGTTGGCTGTCGATGTAAAAATTTGCCAACGATTTGAA‑CACGACCGGATACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGt                                                <  4:912891/138‑1 (MQ=255)
                                                                                  tgacGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACTAAACGGTTGCCGTGCGAGt                                                >  3:276523/1‑138 (MQ=255)
                                                                                  tgacGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGt                                                >  4:788465/1‑138 (MQ=255)
                                                                                  tgacGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGa                                              >  8:885402/1‑140 (MQ=255)
                                                                                   ccctatttatttTGCATCGGTTGGCTGTCTATTTATAATTTAGCCACCTATTTCAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGaa                                             <  5:320619/136‑1 (MQ=255)
                                                                                   cccGATATACGTTTCAACGGTGGGGTGTCGATGTATAATTTTGACAACGATTCTAC‑CACGACCGGTTACGCTGATACGCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACTGTTGCCTTGCGAGTGaa                                             <  6:650353/138‑1 (MQ=255)
                                                                                          tACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACtattat                                      >  1:972021/1‑140 (MQ=255)
                                                                                               ttCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGa                                 >  7:964447/1‑140 (MQ=255)
                                                                                                tCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGAGAACTATTATATCGAg                                >  7:314744/1‑140 (MQ=255)
                                                                                                 cAACGGGTGGCTGCTCATGTATAAATTACCCAACGATTTCAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAgc                               <  7:504241/140‑1 (MQ=255)
                                                                                                  aaCGGTTGGCTGTCGATGTATAAATTGCCCAACGATTTCAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAgcg                              <  8:588136/140‑1 (MQ=255)
                                                                                                  aaCGGGGGGCTGGCGATTTATATATTGCCCAACTTTTTCAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAg                                <  3:536355/138‑1 (MQ=255)
                                                                                                      gtgggCTGGCGAGGTAAAAATTTGCCACCGATTGCAA‑CACGACCGTTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGt                          <  5:258841/137‑1 (MQ=255)
                                                                                                      gTTGGCTGTCGATGTATAAATTTGCAAACTATTTCAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGt                          <  1:542081/140‑1 (MQ=255)
                                                                                                        tGGGTGTCTATTTATAAATTTGCCACCTATTTCAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGttt                        <  6:170678/140‑1 (MQ=255)
                                                                                                        gggatGTCGATTTATAATTTTGAAACCGATTTTAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCTAGTGAACTATTATCTCGAGCGCGGttt                        <  6:749225/136‑1 (MQ=255)
                                                                                                        gggCTGACCATTTATAAATATGCCAACGATTAGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGttt                        <  2:291042/139‑1 (MQ=255)
                                                                                                         ggCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGCGCGCGGtt                         >  4:636054/1‑138 (MQ=255)
                                                                                                         ggCTGACGATCTAAAAATTTGCCAACGATTTGAA‑CACGAACGGTTACGCTGATACACGTGTAGAAACGGAAAAGGGTCTGCAATATACCTTCAACGAAACGGTAGCCTTAAGACAGAACTATTATCTCGAGCTCGGtt                         >  4:296256/1‑138 (MQ=255)
                                                                                                          gCTTTCTATGTATAATTTTGCCACCGATTTTAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGttt                        <  3:140432/138‑1 (MQ=255)
                                                                                                            gttacttGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAat                    <  7:635231/134‑1 (MQ=255)
                                                                                                             gTCGATGTATAAATTTGCCAACGTTTTTAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAata                   <  1:1059539/140‑1 (MQ=255)
                                                                                                             gTCGATGTATAAA‑TTGCCAAACTTTTGAACCACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACTGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAata                   <  5:756316/140‑1 (MQ=255)
                                                                                                                      taAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATa          >  1:120941/1‑140 (MQ=255)
                                                                                                                       aaaTTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGACCTATTATCTCGAGCGCGGTTTCAATATGgatgat           >  3:636284/1‑138 (MQ=255)
                                                                                                                         aTTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGCAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGcc       >  2:293898/1‑140 (MQ=255)
                                                                                                                         aTTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGcc       >  6:161117/1‑140 (MQ=255)
                                                                                                                            tGCCAACTATTTCAC‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCg      <  4:1156606/138‑1 (MQ=255)
                                                                                                                            tGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTTCAATATACCTTCACCGCAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCa    >  7:1076982/1‑140 (MQ=255)
                                                                                                                            tGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCa    >  2:370759/1‑140 (MQ=255)
                                                                                                                            tGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCa    >  6:1095022/1‑140 (MQ=255)
                                                                                                                            tGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTCCAATATACCTTCAACCAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCa    >  7:651130/1‑140 (MQ=255)
                                                                                                                             gCCCACGATTTGAA‑CACGACCGGTGACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCaa   <  8:615152/140‑1 (MQ=255)
                                                                                                                              ccAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCa    >  4:927899/1‑138 (MQ=255)
                                                                                                                              ccAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCAAt  >  5:728410/1‑140 (MQ=255)
                                                                                                                              ccAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCAAt  >  7:33448/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                
ATCACTACGTTGATGAACCGGGTAAAGACACGGCGAATATGCAGCGCTGGAAAATTGCGCCAGACTGGGATGTGAAACTGACTGACGATTTACGTTTCAACGGTTGGCTGTCGATGTATAAATTTGCCAACGATTTGAA‑CACGACCGGTTACGCTGATACCCGTGTCGAAACGGAAACGGGTCTGCAATATACCTTCAACGAAACGGTTGCCTTGCGAGTGAACTATTATCTCGAGCGCGGTTTCAATATGGATGATAGCCGCAAT  >  NZ_CP022686/5001026‑5001291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A