Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A30 F2 I0 R1	181	7914.3	8336376	98.4%	8202993	136.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	1,633,861	A→T	13.6%	L248* (TTA→TAA)	yqeB ←	selenium‑dependent molybdenum cofactor biosynthesis protein YqeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	1,633,861	0	A	T	13.6%	328.6 / 10.7	154	L248* (TTA→TAA)	yqeB	selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
Reads supporting (aligned to +/- strand): ref base A (53/77); new base T (20/2); total (75/79)
Fisher's exact test for biased strand distribution p-value = 1.10e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

CACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAGGAAAAGCTG  >  NZ_CP022686/1633722‑1633994

cACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTa                                                                                                                                        <  3:561452/140‑1 (MQ=255)

   tttAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAata                                                                                                                                     >  7:984314/1‑140 (MQ=255)

     tAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGGTATTTTTGACC‑GGTAATAtt                                                                                                                                   >  2:45104/1‑140 (MQ=255)

     tAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGACCGCAAGAATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATATTTAAAC‑TGTAatata                                                                                                                                   >  3:933290/1‑139 (MQ=255)

        aaCCCGCACGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTCCACC‑TGTAATATTTCt                                                                                                                                <  7:291899/140‑1 (MQ=255)

             gCATGATACAGTCGTAGCGCTACACCACTGGCGATATCCCCCGCACAGCGAATCACCACCAGCTGGTGTCCGGAAGTATGAGAAGTCTTCATCATCAACCCTCCCGGAGCGTTATTTTTCACC‑TGTAATATTTCTGCCAg                                                                                                                           <  6:893164/140‑1 (MQ=255)

             gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCCCCACCAGCTGGGGTACGGAAGGATGAGAAGGCTTCATCCTAAACCCCCCCGGCGCGGTATTATTACAC‑TGTAATAGTTCTGCCAg                                                                                                                           >  7:64223/1‑140 (MQ=255)

             gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAg                                                                                                                           <  5:509878/140‑1 (MQ=255)

             gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAg                                                                                                                           <  8:838713/140‑1 (MQ=255)

             gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAg                                                                                                                           <  1:375496/140‑1 (MQ=255)

             gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGATTTTTTACC‑GGGAATTTTTATGCCAg                                                                                                                           >  3:509639/1‑140 (MQ=255)

             gCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGATTTTTAACC‑AGGAATAATTCTTCCAg                                                                                                                           >  4:319548/1‑140 (MQ=255)

             gCATGATACAGAAGTAGCGCTACACCACTGGCGATCTCCCCCGCACCGAGAATCACCACCATCTTGTGTCCGGAAGGATGAGAAGTCTTCATCATCAACCCACCCGTCGCGTTATTTTTCACC‑TGTAATATTTCTGACAg                                                                                                                           <  6:907048/140‑1 (MQ=255)

             ccATGATACAGAGGTAGCGCTCCAACACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGACAg                                                                                                                           <  7:247165/139‑1 (MQ=255)

               aTGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCa                                                                                                                         <  2:1073493/140‑1 (MQ=255)

               aTGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCa                                                                                                                         <  3:894634/140‑1 (MQ=255)

               aTGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCa                                                                                                                         <  3:915297/140‑1 (MQ=255)

                    aCAGACGTAGCGCTACACCTCTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGCAGGCTTCATCATCAACCCACCCGGCGCGTGATTTTTTAGC‑AGGTATATTTATTCCAGccacctt                                                                                                                    >  1:1021167/1‑134 (MQ=255)

                        aCGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGTCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACC‑GTGAATATTTATCGCAGCCCGCTAAAgg                                                                                                                >  7:564646/1‑140 (MQ=255)

                        aCGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTGACC‑GTTAATATTTAGGCCAGCCCGCTAATgg                                                                                                                >  6:474563/1‑140 (MQ=255)

                        aCGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGATTCAACATCACCCCACCCGGCGCGTTATTTTAAACC‑AGAAATATATAATCCAGCACGCaaaaag                                                                                                                >  8:209338/1‑137 (MQ=255)

                          gTAGCGCTACACCACTGGCGATCTCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCg                                                                                                              <  2:440347/140‑1 (MQ=255)

                          gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCg                                                                                                              <  4:407970/140‑1 (MQ=255)

                          gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCg                                                                                                              <  4:883143/140‑1 (MQ=255)

                          gTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTCCCAGCACGCTAATGGCg                                                                                                              <  4:542664/140‑1 (MQ=255)

                           tAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGTGAAGGCTTGAGCATCAACACACCCGGCGCGGGATGTTTAACC‑GGTGTTAGTTTTGCCAGCCCGCGTATGGCgg                                                                                                             >  6:200327/1‑139 (MQ=255)

                             gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCCCCGGCGCGTTTTTTTTGCCC‑GGTTATATTTATGGCAGCCCGCGAATGGCGAtc                                                                                                           >  5:349854/1‑140 (MQ=255)

                             gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTAACC‑AGTATTATTTCTGCCAGCACGCGAATGTCGCtc                                                                                                           >  5:852987/1‑140 (MQ=255)

                             gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAACC‑TGTTATATTTCTTCCCCCACGCTAAAGGCGAta                                                                                                           >  5:947974/1‑139 (MQ=255)

                             gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAAAC‑TGTAAATTTTGTTCCAGCACGCTAATGTCgcta                                                                                                           >  7:954709/1‑137 (MQ=255)

                             gcgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCACCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGATTTTTAAAC‑TGAAAAATTTTTACCCCCCCACTAATggggcta                                                                                                           >  3:496178/1‑135 (MQ=255)

                              cgcTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTCACC‑TGTAATATTTATGCCAGCACGCAAATGGCGctct                                                                                                          >  6:83748/1‑140 (MQ=255)

                                cTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACC‑GGTAATATTTATGGCCGCACGCTAATGGCCGTCTcc                                                                                                        >  6:135402/1‑140 (MQ=255)

                                   caccacTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTGAAC‑AGTTAATTTTTTTACAGCACGCAAAAGGGGCTCTCCAgc                                                                                                     >  1:568012/1‑140 (MQ=255)

                                       acTGGCGATATCCCCCGCACCGCGAATCACCACCATCTGGTGTCCGGTAGGATGAGAAGGCTTCATCATCACCCCACCAGGCGCGTTATTTTTCACC‑TGTAATATTTCTCCAAGCAAGCTAATGGCGATCTCCTgcgg‑cg                                                                                                <  5:611875/140‑1 (MQ=255)

                                        cTGGCGATATCCCCCGCACCGCGAATCACCCCCAGCTGGTGTCCGGAAGGATGAGAAGGATTCATCATCAACCCACCCGGCGCGGTTTTTTTCGCC‑GGGATTATTTATGACAGCACGCGAATTGGGATCTACTgcgg‑cgg                                                                                               >  2:481743/1‑139 (MQ=255)

                                        cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCCCCGGCGCGGTTTTTTTGCAC‑AGTAATATTTATGCCAGCACGCTTATGGGGGTCTCCTgcgg‑cgg                                                                                               >  1:741248/1‑139 (MQ=255)

                                        cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCCCAGGCGCGTTATTTTTAAAC‑TGGAAAATTTCTGACCGCCAGCTAAATGAGATCTCCTgcgg‑cgg                                                                                               >  8:760585/1‑139 (MQ=255)

                                        cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTGACCGTGT‑ATATTTCTTCCCGAACGAGAATTGGCATATCCTGCTG‑CGt                                                                                               >  8:960354/1‑140 (MQ=255)

                                        cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGGAATATTTCTGCCAGCACGCTAAAGGCGCTCTACAGCGG‑GGt                                                                                               >  6:572251/1‑140 (MQ=255)

                                        cTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCAGCATCACCCCACCCGGCGCGGGTTTTTTGACC‑GGTTATAGATATGGCCGCCCGCGAAGGGCGGTCGCCGgcgg‑cgg                                                                                               >  5:339704/1‑139 (MQ=255)

                                           gCGATTTCCGCGGCACCGCGAATGACCTCCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCCCCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGTTCTCCTGCGG‑CGTTTc                                                                                            <  6:302893/140‑1 (MQ=255)

                                           gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCCCGCTAATGGCGATCTCCTGCGG‑CGTTTc                                                                                            <  6:466734/140‑1 (MQ=255)

                                           gCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTc                                                                                            <  1:979265/140‑1 (MQ=255)

                                             gATATCCCCCTCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCCTCATGATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCg                                                                                          <  8:944654/140‑1 (MQ=255)

                                             gATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCg                                                                                          <  1:835883/140‑1 (MQ=255)

                                              atatCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTGACC‑GTTTATATTTGTGCCAGCACGCTAACTGCGATCACCTgcgg‑cgggtgcgc                                                                                         >  3:67482/1‑133 (MQ=255)

                                                atCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTGACC‑AGTAATATTTATGCCAGCCCCCGAATTGCGATCTCCTGCGG‑CGTTTACGccc                                                                                       >  2:184159/1‑138 (MQ=255)

                                                atCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTAACC‑GTTTATATTTATGCCAGCCCCCGAATGGCGTACTCCGGCGG‑CGGTTCCGCAc                                                                                       >  1:693560/1‑140 (MQ=255)

                                                atCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACC‑AGTAATATTTATTCCAGCAACCGAATTTCGATCTCCTGCGG‑CGTTTACGcca                                                                                       >  3:780655/1‑138 (MQ=255)

                                                atCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGGTTTTTTATCC‑GGTAATATTTATGCCAGCACGCTAAAGGCGCTCTCCTGCGG‑CGGTTgcgccc                                                                                       >  2:33338/1‑138 (MQ=255)

                                                 tCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCAcc                                                                                      <  4:452874/140‑1 (MQ=255)

                                                 cagcccGCCACGCGAATAACCACCATCTGGTGTACGGACGGATGAGAAGGCTTCATCATCAACCCAACAGGCGCGGGATTTAGCACC‑TTTAATATTTCTGCCCGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCAcc                                                                                      <  2:390770/137‑1 (MQ=255)

                                                  cccccGCCCCGCGAAGCTCCACCAGCTGGTGTCCTGTAGTATGAGAAGGCATCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCt                                                                                     <  2:511079/140‑1 (MQ=255)

                                                  cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCt                                                                                     <  3:453353/140‑1 (MQ=255)

                                                  cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCt                                                                                     <  3:307754/140‑1 (MQ=255)

                                                  cccccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCt                                                                                     <  4:167154/140‑1 (MQ=255)

                                                  cccccGCACCGCGAATCACCACCAGCTGGGGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCt                                                                                     <  4:821300/140‑1 (MQ=255)

                                                     ccGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTCACC‑AGTAATATTTGTTCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTACGCCCCTAtg                                                                                  >  4:307754/1‑140 (MQ=255)

                                                             cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGGTATTTTTGACC‑GGTAATATTTCTGGCCGCCCGCGTAAGGCGGGCTCCGGCGG‑CGGTTCCGCCCCAAtgttggtacc                                                                          >  8:346649/1‑135 (MQ=255)

                                                             cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTTTTTTTACCC‑TGTTATATTTATTCCAGCCCCCGAATGGCGCTCTACAGCGG‑CGTTTACGCCCCGTTGTTGTacac                                                                          >  4:350741/1‑137 (MQ=255)

                                                             cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTTTTTTTTACC‑TGGATTAATTATTCCCGCCCCCGTATGGCGGGCTCGGGCGG‑CGGTTGCGCCCCGATGTTGTAAcc                                                                          >  1:11050/1‑140 (MQ=255)

                                                             cgAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTGACC‑GGTTATATTTATGCCCGCCCGCGAATGGCGCTCTACAGCGG‑CGGTTGCGCCCCTATGTTGTATcc                                                                          >  3:521577/1‑140 (MQ=255)

                                                              gAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCa                                                                         <  3:779645/140‑1 (MQ=255)

                                                               aaTCACCAACAGCTTGTTTCCTGAATGATGAGAAGGATTCATCATCAACACACCGGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGTTCTCCTGCGT‑AGTTTCCGCACCTATGTTGTAACCaa                                                                        <  5:940711/140‑1 (MQ=255)

                                                                   cccaccaGCTGGGGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACggg                                                                    <  6:365338/139‑1 (MQ=255)

                                                                   accaccAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACggg                                                                    <  3:748256/140‑1 (MQ=255)

                                                                   accaccAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACggg                                                                    <  3:890531/140‑1 (MQ=255)

                                                                   accaccAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCCGCGCTTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACggg                                                                    <  6:713837/140‑1 (MQ=255)

                                                                   accacaAGCTGGTGTCCGGAAAGATGAGAAGTCTTCATCATCAACCCACACGGCTCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACggg                                                                    <  7:283829/140‑1 (MQ=255)

                                                                       ctttgtGGTGTCCGGAAGGATGTGAAGGCGTCATCAGCTTCCCACCCGGGGCGTTATTTTTCACC‑TGTTATTTTTCTGCGAGAACGCTAATGGCGATCTGGTGCGG‑CGTTTCCGAACCTTTGTTGTAACCTACGGGCGCa                                                                <  4:41393/135‑1 (MQ=255)

                                                                            tGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTaa                                                           <  7:785882/140‑1 (MQ=255)

                                                                             ggTGTCCGGAAGGATGAGTAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTaaa                                                          <  7:843413/140‑1 (MQ=255)

                                                                             ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTaaa                                                          <  5:711568/140‑1 (MQ=255)

                                                                             ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTaaa                                                          <  4:120517/140‑1 (MQ=255)

                                                                             ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTaaa                                                          <  2:588717/140‑1 (MQ=255)

                                                                             ggTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGAGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGTGCGCATGTaaa                                                          <  8:452908/140‑1 (MQ=255)

                                                                             gctgtCCGGAAGGATGAGAAGGCTTCATCATCACCCCACCCGGCGCGTTATTATTCACC‑TGTAATATTTCTGCCAGCACGATAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTCACCAACGGGCGCATGTaaa                                                          <  4:750431/138‑1 (MQ=255)

                                                                                 tCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACggg                                                      <  3:138728/140‑1 (MQ=255)

                                                                                   cGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCa                                                    <  4:893504/140‑1 (MQ=255)

                                                                                     gAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAat                                                  <  7:209338/140‑1 (MQ=255)

                                                                                      aaGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAata                                                 <  8:984314/140‑1 (MQ=255)

                                                                                        ggATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATg                                               <  8:178164/140‑1 (MQ=255)

                                                                                           tGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTTACC‑AGTAATATTTGTGCCAGCACGCTAAAGCGGGACTACTGCGG‑CGTTTCCGCAACAATTTTGTAACCCCCCGGCGCCAGTTAACGGGGAATATGtta                                            >  2:595600/1‑139 (MQ=255)

                                                                                           tGAGAAGGCTTCATCATCAACCCACCCGGCGCGGTATTTTTAACC‑AGTAATATTTATGCCAGCACCCGAATTGCGCTCTCCTGCGG‑CGGTTACGCACCTATGTTGTAAACAAACGGCGCATGTAAACGGGCAATAtttta                                            >  2:438451/1‑139 (MQ=255)

                                                                                            gagaAGGCTTCAGCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTTTGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGttct                                           <  1:812654/140‑1 (MQ=255)

                                                                                             agaAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGttctt                                          <  2:741248/140‑1 (MQ=255)

                                                                                                 ggCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGc                                      <  4:984760/140‑1 (MQ=255)

                                                                                                   cTTCATCCTCAACCCACCCGGCGCGTTATTTTTAACC‑GGTAATATTTATGCCACCACCCTTATGGAGCACACCAGAGG‑CGTTAACCCACAAATGTTGTAAACAAACGGCGCAAGTAAAAGGGCTATATTttattaaccac                                    >  2:394959/1‑134 (MQ=255)

                                                                                                   cTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCa                                    <  5:531175/140‑1 (MQ=255)

                                                                                                    ttCATCATCAACCCACCCGGCGCGTTTTTTTTAACC‑TGTTATAATTATGCCAGCACCCGAATTGCGATCACCTCCGGCCGTTACCCCA‑CTATGTTTTAACCAACGGGCGCAAGTAAAAGGGGAATATGTTCTTCACCCAc                                   >  2:80660/1‑140 (MQ=255)

                                                                                                    ttCATCATCAACCCACCCGGCGCGTTATTTTTGACC‑TGTAATATTTCTGGCAGCACGCGAAAGGCGATCTCCAGCGG‑CGTTTCCGCCCCTATGTTGTAAACAACCGGCGCATGTAAACGGGCAATATGTTATTCAGCCAc                                   >  6:794747/1‑140 (MQ=255)

                                                                                                    ttCATCATCAACCCACCCGGCGCGTTATTTTTAACC‑TGTAATATTTCTGCAAGCACTCTAATGGCGATCTCCTGCGG‑CGTTTCCGCAGCTATGTTGTAACCTACGCGCGCATGTAAACGGGCAATATGTTCTTCAGCCAc                                   <  2:993310/140‑1 (MQ=255)

                                                                                                                  acccGGCGCGGTATTTTTCACC‑TGTAATATTTATGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCAGTTAAACGGGAAAAATGTTATTCAGCCACGCCGTTCTCACGCa                     >  8:790591/1‑140 (MQ=255)

                                                                                                                   cccGGCGCGTTATTTTTCACC‑TGTAATATTTATGCCAGCACGCTAATGGCGATCTACTGCGG‑CGTTTCCGCACCTAAGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTATTAAGCCACCCCGTtctacagcaa                    >  2:285455/1‑133 (MQ=255)

                                                                                                                             tATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAgg          <  7:455517/140‑1 (MQ=255)

                                                                                                                             tATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAgg          <  4:849838/140‑1 (MQ=255)

                                                                                                                              aTTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCGGCCACGCCTTTCTCACGCAATTGGCGCAGGa         <  5:84820/140‑1 (MQ=255)

                                                                                                                               tttttCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAGGaa        <  5:186793/140‑1 (MQ=255)

                                                                                                                                    cACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAGGAAAAGCt   <  5:474563/140‑1 (MQ=255)

CACTTTAAAACCCGCATGATACAGACGTAGCGCTACACCACTGGCGATATCCCCCGCACCGCGAATCACCACCAGCTGGTGTCCGGAAGGATGAGAAGGCTTCATCATCAACCCACCCGGCGCGTTATTTTTCACC‑TGTAATATTTCTGCCAGCACGCTAATGGCGATCTCCTGCGG‑CGTTTCCGCACCTATGTTGTAACCAACGGGCGCATGTAAACGGGCAATATGTTCTTCAGCCACGCCTTTCTCACGCAATTGGCGCAGGAAAAGCTG  >  NZ_CP022686/1633722‑1633994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A