Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A28 F2 I0 R1	74	6379.7	5468672	98.4%	5381173	135.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	2,408,861	Δ1 bp	100%	pseudogene (1097/3115 nt)	mdtF →	multidrug efflux pump RND permease MdtF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	2,408,861	0	T	.	99.3%	652.7 / ‑3.2	141	pseudogene (1097/3115 nt)	mdtF	multidrug efflux pump RND permease MdtF
Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (75/65); total (75/66)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 143 total aligned reads displayed.

TGAAGACGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAATTCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCAT  >  NZ_CP022686/2408726‑2408994

tGAAGACGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAAtca                                                                                                                                     <  7:50585/138‑3 (MQ=255)

tGAAGACGGTTTATCCTTACGACACCACACCGATTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAAtca                                                                                                                                     <  8:175258/138‑3 (MQ=255)

   aGACGGTTTATCCTTAAGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcg                                                                                                                               <  2:169601/140‑1 (MQ=255)

     ccGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCAATTCAGGAAGTTTTCAAAACGGTGGTTTAGGCTATCAACCTCGTCTTCCTGGTCATGTATCTTTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcg                                                                                                                               <  7:184650/137‑1 (MQ=255)

       ggTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcgacga                                                                                                                           >  5:572234/1‑140 (MQ=255)

        gTTTATCCTTACTACACCTCTCCTTTTATCAAAATTTCTATTCAGGAAGTTTTCAATTCGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGAt                                                                                                                          <  1:183465/140‑1 (MQ=255)

         tttATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGATGCTATCATCCTCTTCTTCATGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGAtt                                                                                                                         >  5:62927/1‑140 (MQ=255)

         tttATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGATATTCAGCGCCACAA‑TCATCCCGACTAtt                                                                                                                         >  4:2709/1‑140 (MQ=255)

         tttATCCTTACGACACCACACCGTTTATCAAAATTTATATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTGTTGCAGCATTTCCGCGCCACAA‑TCATCCCGCCGAtt                                                                                                                         >  6:575029/1‑140 (MQ=255)

         tatATCCTAACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTATCAAAACGCTCGTTGAGGCTATCATCCACGTCTTACTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGAtt                                                                                                                         >  6:421299/3‑140 (MQ=255)

           tATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGAtt                                                                                                                         <  7:600417/138‑1 (MQ=255)

           tATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGc                                                                                                                       <  5:468738/140‑1 (MQ=255)

            aTCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcgac                                                                                                                             >  2:226949/1‑133 (MQ=255)

            aTCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcgac                                                                                                                             <  1:226949/133‑1 (MQ=255)

             accTTACGACACCACACCGTTTATCAAAATTTCTATTCTGGAAGTTTTCAACACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGc                                                                                                                       <  8:284373/137‑1 (MQ=255)

                 tACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTAcc                                                                                                                 >  1:268597/1‑140 (MQ=255)

                 tACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTAcc                                                                                                                 >  2:346653/1‑140 (MQ=255)

                 tACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTAcc                                                                                                                 >  3:645152/1‑140 (MQ=255)

                  aCGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCg                                                                                                                >  3:231920/1‑140 (MQ=255)

                   cGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTAcc                                                                                                                 >  7:600126/1‑138 (MQ=255)

                   cGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCgg                                                                                                               >  4:663816/1‑140 (MQ=255)

                    gacaaaacaccGTTTATCAAAATTTCTATACAGGAAGTATACAAAACCCTTCTTAAGGAAATAAAAAAAGTCTAAATGCTCATCTAACTTATTTAGCAGCAAATCCGCGCCACAA‑TCATCCCGACGATTCCACTACCg                                                                                                                >  8:219938/7‑138 (MQ=255)

                       accacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCggtggt                                                                                                           >  3:129990/1‑140 (MQ=255)

                       accacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCggtggt                                                                                                           >  3:204419/1‑140 (MQ=255)

                       accacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCggtggt                                                                                                           <  5:670615/140‑1 (MQ=255)

                       accacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTACTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCggtggt                                                                                                           >  5:14901/1‑140 (MQ=255)

                        ccacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCgg                                                                                                               <  2:557753/135‑1 (MQ=255)

                        ccacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCgg                                                                                                               >  1:557753/1‑135 (MQ=255)

                        ccacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTAGTCTTACTGGTCATGTATCTGTTTTTGCTGAATTTCCGCGCCCCAA‑TCAGACCGACGATTGCCGTACCGGTGGtt                                                                                                          >  3:425918/1‑140 (MQ=255)

                         cacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGTTAATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCTTACCggtggt                                                                                                           >  8:230347/1‑138 (MQ=255)

                         cacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGttt                                                                                                         >  5:226265/1‑139 (MQ=255)

                         cacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGAACCGGTGGTTa                                                                                                         >  6:395470/1‑140 (MQ=255)

                         cacaccGTTTATCAAAATTTCTAATCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACCA‑TCATCCCGACGATTGCCGTACAGGTGGTTa                                                                                                         >  5:379006/1‑140 (MQ=255)

                         cacaccGTGTATAAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTAGCATACTCGTCTTCCTTGTCATGTATCTGTGTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCggtggt                                                                                                           <  7:502991/138‑1 (MQ=255)

                           caccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAACCGCTGGTTGAGGCTATCATCCTAGGCTTACTGGTCATGTATCTGTTTTTTCAGAATTTCCGCTCCACAA‑TCATCCCGACTCTTGCAGTACCGGTGCTAAtt                                                                                                       >  6:167712/1‑140 (MQ=255)

                           caccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGAATCTGTTTTTGCAGAATTTCCTCGCCACAA‑TCATCCCGACGATTTCCGTACCggtgtgta                                                                                                         >  8:2846/1‑134 (MQ=255)

                             ccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTAtt                                                                                                       >  8:565156/1‑138 (MQ=255)

                              cGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGCTTGCCGTACCGGTGGTTAtta                                                                                                      >  7:159280/1‑137 (MQ=255)

                                tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTACGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                  >  1:639787/1‑140 (MQ=255)

                                tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATAATCCTCGTCTTACTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATACCGCCGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                  >  4:199560/1‑140 (MQ=255)

                                 ttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCggg                                                                                                 <  1:346977/140‑1 (MQ=255)

                                  tATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGATATTCTCgg                                                                                                  >  7:72594/1‑138 (MQ=255)

                                         aTTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGc                                                                                         <  6:80621/140‑1 (MQ=255)

                                                 tCATGATGTTTTGAAAACGCTGGTTGAGGCTTTCATCCTGGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCAACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGTCGTTTGCGATCTTGt                                                                                 <  5:215525/140‑1 (MQ=255)

                                                      aaGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTcggcg                                                                            <  2:7568/140‑1 (MQ=255)

                                                        gTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTTGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGcggt                                                                          <  5:314718/140‑1 (MQ=255)

                                                          tttCAAAACGCTGGTTGAGGCGATCATCCGCGTCTTCCTGGGCATGTCTCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGGTATTCTCGGGACGTTTGCGATCTTGTCGGcggtcg                                                                        <  5:152590/140‑1 (MQ=255)

                                                                aaCGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGttt                                                                    <  8:600126/138‑1 (MQ=255)

                                                                     tGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATc                                                             >  3:42434/1‑140 (MQ=255)

                                                                     tGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATc                                                             <  3:663816/140‑1 (MQ=255)

                                                                     tGGTTGAGGCTATCATACTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATc                                                             <  4:279289/140‑1 (MQ=255)

                                                                       gTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCaa                                                           <  3:73688/140‑1 (MQ=255)

                                                                       gTTGAGGCTATCATCCTCGTCTTCCTGGGCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCaa                                                           <  1:266613/140‑1 (MQ=255)

                                                                       gTTGAGGATATCAGCCTAGGATTATTTGTGATGTATCTGTTATTGCAGAATTTCCGCTCCTCAA‑TCATCCCTACGATTGACGTTCAGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATc                                                             <  8:278621/138‑1 (MQ=255)

                                                                             gCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGtt                                                     >  6:348362/1‑140 (MQ=255)

                                                                               tATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTaa                                                   <  2:134454/140‑1 (MQ=255)

                                                                                atcatcCTCTTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAAc                                                  <  4:370888/140‑1 (MQ=255)

                                                                                 tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                 >  2:503984/1‑140 (MQ=255)

                                                                                 tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                 >  2:54946/1‑140 (MQ=255)

                                                                                 tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                 >  3:324617/1‑140 (MQ=255)

                                                                                      cTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGtt                                            <  5:503871/140‑1 (MQ=255)

                                                                                       tCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCACCACGTTAACTATGtgc                                           >  4:272956/1‑138 (MQ=255)

                                                                                       tCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCACCACGTTAACTATGTTc                                           >  5:433020/1‑140 (MQ=255)

                                                                                       tCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTc                                           >  6:368787/1‑140 (MQ=255)

                                                                                         gTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGtta                                           >  7:584471/1‑137 (MQ=255)

                                                                                         gTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCgg                                         >  1:711315/1‑140 (MQ=255)

                                                                                           cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCgg                                         <  8:471508/138‑1 (MQ=255)

                                                                                           cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                       <  3:319662/140‑1 (MQ=255)

                                                                                           cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                       <  3:531407/140‑1 (MQ=255)

                                                                                           cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                       <  3:546697/140‑1 (MQ=255)

                                                                                           cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGGGGGTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                       <  5:546663/140‑1 (MQ=255)

                                                                                           attCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCAACAA‑TCATCACGACGATTGCCGTACAGGTGGTTTTTCTCGGGAAGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                       <  4:268708/139‑1 (MQ=255)

                                                                                              ccTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATgg                                    >  2:189769/1‑140 (MQ=255)

                                                                                                 tgTCATGTATCTGTTTTTGCAGAATTTCCGCTCAACAA‑TCAACCCGACGATTTCAGTACCGGTGGTTATTCTCGGGAAGTTTGAGATCTTGTCGTCTGTCTGTTTCACCATCAACACGTTAACTATGTTAGGGATGGTGc                                 <  4:133942/139‑1 (MQ=255)

                                                                                                 ggTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGc                                 >  4:570319/1‑140 (MQ=255)

                                                                                                 ggTCATGTATCTGTTTTTGCACAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGc                                 <  3:390928/140‑1 (MQ=255)

                                                                                                  gTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTg                                  <  7:78974/138‑1 (MQ=255)

                                                                                                     ctGTATCAGGTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCTGTCGTTTTCACCTTCAACACGTTAACTATGTTCGTGATGGTGCTGGc                             <  2:470045/139‑1 (MQ=255)

                                                                                                       gTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGa                           >  1:150750/1‑140 (MQ=255)

                                                                                                       gTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACCTTTGCGAACTGGTCGGCGGTCGGTTTCACCAACAACACGATAACTATTTTAGGGATGGTGCAGGCGa                           >  6:651894/1‑140 (MQ=255)

                                                                                                        tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGAt                          <  2:711315/140‑1 (MQ=255)

                                                                                                        tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGAt                          <  6:514657/140‑1 (MQ=255)

                                                                                                        tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGAAACGGTGGTTATTCTCGGGACGTTTGCGATATTGTCGGCGGTCGGTTTCACAATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGAt                          >  4:530025/1‑140 (MQ=255)

                                                                                                        tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCAGAAGATTGGCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGAt                          <  2:116388/140‑1 (MQ=255)

                                                                                                         aTCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGa                           <  8:130956/138‑1 (MQ=255)

                                                                                                            tGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAggg                      >  6:658622/1‑140 (MQ=255)

                                                                                                            tGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGAGGGTGCTGGCGATAggg                      >  4:23486/1‑140 (MQ=255)

                                                                                                             gTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                       <  2:228778/122‑1 (MQ=255)

                                                                                                             gTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGt                     <  6:14901/140‑1 (MQ=255)

                                                                                                             gTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGt                     <  4:129990/140‑1 (MQ=255)

                                                                                                                tttGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTAc                  <  4:674169/140‑1 (MQ=255)

                                                                                                                tttGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGAGCTTGTCGGCGGTCGGTTTAAGCATCAACACGTTTACTATGGTCGGGATGGTGCTGGCGATAGGGTTAc                  >  6:542313/1‑140 (MQ=255)

                                                                                                                 ttGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACt                 >  6:473824/1‑140 (MQ=255)

                                                                                                                  tGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                       <  4:192384/117‑1 (MQ=255)

                                                                                                                  tGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACtg                >  4:452626/1‑140 (MQ=255)

                                                                                                                    cAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCCCCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACtggt              >  4:364039/1‑140 (MQ=255)

                                                                                                                       aaTTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGa           <  1:39693/140‑1 (MQ=255)

                                                                                                                             cgcgCCTCAA‑TCATCCCTACGATTGCCGTTCCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGGCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGc     <  6:196342/140‑1 (MQ=255)

                                                                                                                                gcCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGGCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTTCTGGCGATAGGGTTACTGGTGGATGACGCCAt  <  5:196525/140‑1 (MQ=255)

                                                                                                                                  cacaA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCGTGTCGGCGGTCGGTTTCACCATCAACACGTTCACTATGTTCGGGATGGTGCTGGCGATAGGGGTACTGGTGGATGACGCCAt  <  8:429427/138‑1 (MQ=255)

TGAAGACGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAATTCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCAT  >  NZ_CP022686/2408726‑2408994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A