Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A30 F2 I0 R1	181	7914.3	8336376	98.4%	8202993	136.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	2,408,861	Δ1 bp	100%	pseudogene (1097/3115 nt)	mdtF →	multidrug efflux pump RND permease MdtF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	2,408,861	0	T	.	98.7%	808.7 / 1.4	184	pseudogene (1097/3115 nt)	mdtF	multidrug efflux pump RND permease MdtF
Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (84/97); minor base C (1/1); total (85/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 184 total aligned reads displayed.

AAGACGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAATTCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCG  >  NZ_CP022686/2408728‑2408996

aaGACGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATccc                                                                                                                                  >  5:687221/1‑140 (MQ=255)

aaGACGGTTTATCCTTACGACACAACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATATGTTTTTGCAGAATTTCCGCGCCACAA‑TCATccc                                                                                                                                  >  3:481306/1‑140 (MQ=255)

   ccGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCACGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcgac                                                                                                                               <  2:951887/139‑1 (MQ=255)

   aaggTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATACTCGTCTTCCTTGTCATTTATCTGTTTTTGCAGAATTTCCGCGCCACTA‑TCATCCcgac                                                                                                                               <  5:191085/138‑1 (MQ=255)

   aCGGTATATCCTTACTACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTGAAACCGCTGGTTGAGGCTATCATCGTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcgac                                                                                                                               <  8:857757/140‑1 (MQ=255)

     ggTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcgacga                                                                                                                             >  5:414636/1‑140 (MQ=255)

     ggTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCcgacga                                                                                                                             >  4:279246/1‑140 (MQ=255)

     ggTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCATGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCAC‑ACTCATCCcgacga                                                                                                                             >  3:964246/1‑140 (MQ=255)

      gTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGAt                                                                                                                            <  4:588942/140‑1 (MQ=255)

         tATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGc                                                                                                                         <  8:419038/140‑1 (MQ=255)

         tATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGc                                                                                                                         <  2:242603/140‑1 (MQ=255)

               tACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGCAGATTGCAGTAcc                                                                                                                   >  2:317594/1‑140 (MQ=255)

               tACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTAcc                                                                                                                   >  6:91346/1‑140 (MQ=255)

               tACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTAcc                                                                                                                   <  4:156336/140‑1 (MQ=255)

               tACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGAGCCACAA‑TCATCCCGACGATTGCAGTAcc                                                                                                                   >  3:5926/1‑140 (MQ=255)

               tACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATAATCCTCTTCTTCCTGCTCATGGATCTGTTTATGCATAATTTACTCTCCACAA‑TCATCCAGACGATTCCCGTaac                                                                                                                   >  4:820137/1‑138 (MQ=255)

               tACGACACCACACCGTTGATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCAGCATCGTCTTCCTGGTCAGGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTTcc                                                                                                                   >  5:282270/1‑140 (MQ=255)

               tACGACACCAAACCGTAAATCAAAATTTCTATTCAGGAAGTTTTCAACACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTTTTTTTGCAGAATATCCGCGCCCCAA‑TCATCCCGACGATAGCCGTAcc                                                                                                                   >  2:654628/1‑140 (MQ=255)

                aCGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCg                                                                                                                  <  7:352434/140‑1 (MQ=255)

                     accacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCGGTTTTTGGAGAATTTCCGCGCCACAA‑TCATCCAGAAGATTGACGTACCggtggt                                                                                                             >  5:998525/1‑140 (MQ=255)

                      ccacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGtt                                                                                                            >  7:895969/1‑140 (MQ=255)

                      ccacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAAAGCTGGTTGATGATATCATCCTCGTCATCATGGTCATGTATCTGTTTTTTCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGtt                                                                                                            >  7:2432/1‑140 (MQ=255)

                       cacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAACACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCACGACGATTGCCGTACCGGTGGttt                                                                                                           >  5:1008265/1‑139 (MQ=255)

                       cacaccGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTa                                                                                                           <  7:221696/140‑1 (MQ=255)

                       cacaccGTTTATCAAAAATTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTa                                                                                                           <  7:56873/140‑1 (MQ=255)

                            cGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATtctc                                                                                                      >  4:648956/1‑140 (MQ=255)

                              tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  2:622853/1‑140 (MQ=255)

                              tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  8:579872/1‑140 (MQ=255)

                              tttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  4:196475/1‑140 (MQ=255)

                              tttATCAAAATTTCCATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  5:417180/1‑140 (MQ=255)

                               ttATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCggg                                                                                                   <  2:545111/140‑1 (MQ=255)

                                tATCAAAGTTTCTATTCAGGAAGTTTTCAAAACGCAGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgggc                                                                                                  >  8:335775/1‑139 (MQ=255)

                                tATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGa                                                                                                  >  6:763769/1‑140 (MQ=255)

                                tATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGa                                                                                                  <  2:893934/140‑1 (MQ=255)

                                tATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTAGCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGa                                                                                                  <  7:268531/140‑1 (MQ=255)

                                 aTCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGAc                                                                                                 >  3:607226/1‑140 (MQ=255)

                                 aTCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGAc                                                                                                 >  1:78571/1‑140 (MQ=255)

                                      aaTTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTg                                                                                            <  1:63040/140‑1 (MQ=255)

                                        tttCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTTGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACTA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCg                                                                                          <  3:780046/140‑1 (MQ=255)

                                        tttCTATTCAGGAAGTTTTCAAAACGCTGGGTGAGGCTATCATCCTCGGCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCg                                                                                          <  2:447418/140‑1 (MQ=255)

                                                        tttCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTCCCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGcggtcg                                                                          >  8:109540/1‑140 (MQ=255)

                                                           aaaaaCGCTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCACGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGtt                                                                       <  7:337087/139‑1 (MQ=255)

                                                            aaaaCGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGttt                                                                      <  3:933863/140‑1 (MQ=255)

                                                            aaaaCGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGttt                                                                      <  7:736019/140‑1 (MQ=255)

                                                              aaCGCTGGTTGAGGCTCTCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCa                                                                    <  6:817941/140‑1 (MQ=255)

                                                                   tGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATc                                                               <  7:46261/140‑1 (MQ=255)

                                                                   tGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATc                                                               >  5:884145/1‑140 (MQ=255)

                                                                   tGGTTGAGGATATCATCCTCGTCTTCCTTGTCATGTATATGTTTTTGCAGAAATTCCGCGCCACAA‑TCATCCAGACGATTGCCGTACCGGTGTTTATTCTCGGGACGTTTGAGATCTTGTCGGCTGTCGGTTTCACCATc                                                               <  8:775845/140‑1 (MQ=255)

                                                                     gtggAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGACTTTCCGCGCCACAA‑TCATCGCGACGATTGCCGTACCGGTGGTTCTTCTCGGGACGTGTGCGATCTTGTCGGCGGTCGGTTTCACCATCaa                                                             <  3:191257/137‑1 (MQ=255)

                                                                     gTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCTCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTTTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCaa                                                             <  7:662281/140‑1 (MQ=255)

                                                                          ggCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGGTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGt                                                        <  3:788431/140‑1 (MQ=255)

                                                                          ggCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACAGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGt                                                        <  3:255594/140‑1 (MQ=255)

                                                                             tATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCCTCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCTACACGTTaa                                                     <  2:602023/140‑1 (MQ=255)

                                                                             tATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTACGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTaa                                                     <  7:579872/140‑1 (MQ=255)

                                                                             tATCATCCTCGGCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACCCGTTaa                                                     <  5:441370/140‑1 (MQ=255)

                                                                               tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACTATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTaaat                                                   >  4:351319/1‑138 (MQ=255)

                                                                               tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                   >  5:511946/1‑140 (MQ=255)

                                                                               tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                   >  2:686151/1‑140 (MQ=255)

                                                                               tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                   <  2:78571/140‑1 (MQ=255)

                                                                               tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTAACCATCAACACGTTAACt                                                   >  1:487686/1‑140 (MQ=255)

                                                                               tcatcCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGAATGCCGTACCGGTGGTTATTCTCGGGACGGTTGCGATCTTGTCGGCGGTCGGTTTCCCCATCAACACGTTAACt                                                   >  5:882043/1‑140 (MQ=255)

                                                                               tcatcCTCGTCTTCCTGGTCATGTATCTGGTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACt                                                   <  1:1059787/140‑1 (MQ=255)

                                                                                    cTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCgg                                                                                                    >  5:669591/1‑86 (MQ=255)

                                                                                    cTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGtt                                              <  7:380901/140‑1 (MQ=255)

                                                                                     tCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTc                                             >  3:420128/1‑140 (MQ=255)

                                                                                        tCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCggg                                          >  3:835960/1‑140 (MQ=255)

                                                                                         cTTCCTGGTCATGTATCTGTTTTTGCTGAATTTCCGCGCCAC‑ACTCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  7:179203/140‑1 (MQ=255)

                                                                                         cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  4:455148/140‑1 (MQ=255)

                                                                                         cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  3:105512/140‑1 (MQ=255)

                                                                                         cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  8:364919/140‑1 (MQ=255)

                                                                                         cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  2:346096/140‑1 (MQ=255)

                                                                                         cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  1:710181/140‑1 (MQ=255)

                                                                                         cTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGAGCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  6:935443/140‑1 (MQ=255)

                                                                                         cTTCCTGGGCATGTAGCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGa                                         <  6:690101/140‑1 (MQ=255)

                                                                                           tCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATg                                       >  4:1044750/1‑140 (MQ=255)

                                                                                            ccTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATgg                                      >  1:690260/1‑140 (MQ=255)

                                                                                            ccTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATgg                                      >  1:156394/1‑140 (MQ=255)

                                                                                               ggTCATGTATCTGTTTTTGCAGATTTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATTGTGc                                   <  7:138917/140‑1 (MQ=255)

                                                                                               ggTCATGTATCTGTTTTTGCAGAATTTCCGCTCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGTGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATAAACTCGTTAACTATGTTCGGGATGGTGc                                   <  4:911254/140‑1 (MQ=255)

                                                                                               ggTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGc                                   <  1:103575/140‑1 (MQ=255)

                                                                                               ggTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGc                                   <  4:35995/140‑1 (MQ=255)

                                                                                               ggTCATGTATCTGTTATTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGTGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGc                                   <  1:868133/140‑1 (MQ=255)

                                                                                                gTCATGTATCTGTTTTTGCAGAATTTCCGCGCCCCAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGGTTCACCATCAACACGTTAAATATGTTCGGGATGGTGCt                                  >  6:593217/1‑140 (MQ=255)

                                                                                                gTCATGTATATGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATATTGTCGGGGGTCGGTTTCACCATCAACACGTTAACTATGTTAGGGATGGTGCt                                  >  7:291188/1‑140 (MQ=255)

                                                                                                 tgaTGTATCTGTTTTTGCAGAAATTCCGCGCCACAA‑TCATCCAGACGATTGCCGTACCGGTGGTTTTTCTCTGGACGTTTGCGATCATGTCGGCGGTCGGATTCACCAACACCACGTTAACTATGTTCGGGATggagatg                                 >  6:95841/3‑135 (MQ=255)

                                                                                                   aTGTATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTAGGGATGGTGCTGGc                               >  6:364260/1‑140 (MQ=255)

                                                                                                      tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGAt                            <  3:665348/140‑1 (MQ=255)

                                                                                                      tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGAt                            <  4:607226/140‑1 (MQ=255)

                                                                                                      tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGAt                            <  6:512160/140‑1 (MQ=255)

                                                                                                      tATCTGTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGAt                            <  4:775112/140‑1 (MQ=255)

                                                                                                           ttttttGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGt                       <  1:855537/139‑1 (MQ=255)

                                                                                                           gTTTTTGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGt                       >  5:760500/1‑140 (MQ=255)

                                                                                                            tttttGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGtt                      >  2:506066/1‑140 (MQ=255)

                                                                                                               ttGCAGAATTTCCGCGCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACt                   >  1:54970/1‑140 (MQ=255)

                                                                                                                           cgcgCCACTA‑TCATACCTACGATTGCCGTTCAGGTGGTTCTTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTAACCATCATCACGTTAACTATGTTCGGGATGGTGCTGGCTATAGGGTTACTTGTGGATGACGc       <  8:790167/140‑1 (MQ=255)

                                                                                                                           cgcgCCACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGc       <  6:109350/140‑1 (MQ=255)

                                                                                                                           cgcgCCACAA‑TCATCCCGACGATTGCAGTACCGGTGTTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATAAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGc       <  6:255070/140‑1 (MQ=255)

                                                                                                                              gcCACAA‑TCCTCCCGACGATGGCCGTACCGGTGGTTATGCTCGGGACGATAGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTTACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCAt    <  6:424007/140‑1 (MQ=255)

                                                                                                                               ccACAA‑TCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATc   >  2:480315/1‑140 (MQ=255)

                                                                                                                                cacaA‑TCATCCCGACGATTGCAGTACCGTTGGGTATTCTCGGGACGTTTGCGATATTGTCGGCGGGCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCg  <  3:262536/140‑1 (MQ=39)

AAGACGGTTTATCCTTACGACACCACACCGTTTATCAAAATTTCTATTCAGGAAGTTTTCAAAACGCTGGTTGAGGCTATCATCCTCGTCTTCCTGGTCATGTATCTGTTTTTGCAGAATTTCCGCGCCACAATTCATCCCGACGATTGCCGTACCGGTGGTTATTCTCGGGACGTTTGCGATCTTGTCGGCGGTCGGTTTCACCATCAACACGTTAACTATGTTCGGGATGGTGCTGGCGATAGGGTTACTGGTGGATGACGCCATCG  >  NZ_CP022686/2408728‑2408996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A