Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A26 F2 I0 R1 64 5414.3 5027226 98.2% 4936735 134.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,460,576 C→A 14.0% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,460,5760CA14.0% 171.8 / 3.8 92M1M (ATG→ATT) †yhjYautotransporter outer membrane beta‑barrel domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (53/23);  new base A (4/11);  total (57/35)
Fisher's exact test for biased strand distribution p-value = 2.78e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAT  >  NZ_CP022686/2460438‑2460713
                                                                                                                                          |                                                                                                                                          
aCGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCa                                                                                                                                           >  6:489263/1‑140 (MQ=255)
 cGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTCATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTGTTTTTTATGATc                                                                                                                                            >  2:626732/1‑138 (MQ=255)
      tttgGATTTTCCGGGTTGCGAATAAACTATATATTTTTGTTCCCCGGCGTACGCGGTGTTAATAAAAACGCCACTTATAACCACGCCCCAACGAAGGCTTAGCTCCCAGCGACCACCGCTTTTTTTTATTATAATTA‑cca                                                                                                                                    <  6:580231/138‑1 (MQ=255)
      tgaggAATGTCCGGTTTGCGTATCACAGAAATATTATTGTTGCCAGGCGTATGCTGAGTTGAGAAAAAAGGCACTGAATACCAAGCTCGCAAGCAGGCTTAGATGCCAGCGCCAAACGCTTTTTTTTATTAGAATTA‑aca                                                                                                                                    >  5:112932/4‑140 (MQ=255)
        tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑aca                                                                                                                                    >  8:230423/1‑138 (MQ=255)
        tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑aca                                                                                                                                    >  7:480260/1‑138 (MQ=255)
        tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑aca                                                                                                                                    >  2:233345/1‑138 (MQ=255)
        tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑aca                                                                                                                                    >  4:187410/1‑138 (MQ=255)
        tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑aca                                                                                                                                    >  1:406250/1‑138 (MQ=255)
        tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑aca                                                                                                                                    >  8:11142/1‑138 (MQ=255)
        tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACg                                                                                                                                  >  5:540656/1‑140 (MQ=255)
        tgGAATGTCCGGGTTGCGTATCAACGATAAATTCTAGTTGCCAGGCGTAAGCTGAGTTGAGAAAAAAGGCACAGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTATTATTATCATTA‑aca                                                                                                                                    >  1:73921/1‑138 (MQ=255)
         gtattGTCCGGGTTGCGTATCAACTATATATTCTTGTTGCCAGGCGTAAGCTGTGTTTAGAAAAAAGGCACTTATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑acac                                                                                                                                   <  4:555738/134‑1 (MQ=255)
          gAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACg                                                                                                                                  >  1:64219/1‑138 (MQ=255)
             tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTcc                                                                                                                             >  6:564545/1‑140 (MQ=255)
             tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACAGCTTTTTTTTATGATCATTA‑ACACGACTcc                                                                                                                             >  5:592682/1‑140 (MQ=255)
               tCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTcc                                                                                                                             >  7:80103/1‑138 (MQ=255)
               tCCGGGTTGCGTATAAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACAcc                                                                                                                             >  2:539799/1‑138 (MQ=255)
                ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAgg                                                                                                                          >  5:70521/1‑140 (MQ=255)
                 ggggTTGCGTATCAAGTATATTTTCTTGTGGCCGGGCTTATGGTGTTTTTATAAAAAATGCACTTATTACCACGCTCGCACGCAGGCTTAGCTGCCAGCGACCACCTATTTTTTTTATTATAATTA‑ACACGACTCCAg                                                                                                                           <  8:25942/137‑1 (MQ=255)
                 cttgTTGAGAATCAACGATATATTCTTATTGCCAGTCGTAAGCTGTGATGAGAAAAAAGGCACTGATTACCACGCACGCAATCAGGCTTAGCTGCCACCGACCACCTCTTTTTATTATGATCAATA‑ACACGAATCAAg                                                                                                                           >  2:29089/4‑138 (MQ=255)
                  gggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAgg                                                                                                                          >  2:142641/1‑138 (MQ=255)
                   ggTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑TCACGCCTCCAggg                                                                                                                         >  3:66757/1‑138 (MQ=255)
                    gTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGCCTCCAGGGa                                                                                                                        >  2:606930/1‑138 (MQ=255)
                          tATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATTATAATTA‑ACACGCCTCCAGGGACGAGcc                                                                                                                  >  7:505450/1‑138 (MQ=255)
                          tATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGcc                                                                                                                  >  8:536996/1‑138 (MQ=255)
                            tCAACGATATATTCATGTTGCCAGGCGTAAGCTGAGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTATTTTTTAAGATCATTA‑ACACGACTCCAGGGAAGAGCCgc                                                                                                                >  3:497650/1‑137 (MQ=255)
                             aaaCTATATTTTCTTGTGCCCCGGCTTAAGCTGTGTTGAGAAAAACGGCACTTATAACCCCGCGCGCACCCAGGCTTCGCGCCCAGCCACCACCGCTTTTTTTTATCATCATAC‑CCACGACTCCAGGGAAGAGCCGAAtt                                                                                                             <  6:355294/139‑1 (MQ=255)
                                ttatatatTCTTGTTCCCTGGCTTTCGGTGTGTTCATAAACAAGCCACTGAAACCCCCGCTCGCACCCTGGCTTCGCGGCCAGCCACCCCCGATTTTTTTTATCATCATAA‑CCACGACTCCAGGGAAGAGCCGAATTc                                                                                                            <  4:6776/136‑1 (MQ=255)
                                tgATATATTATTTTTGCCCTGCGTAAGGGGTGTTTCCAAAATCGGCACTTATTACCCCGCGCGCCCTGAGTCTGAGCGCCCACCCCCCCCCGTTTTTTTTTTTTCTCTTTA‑ACACGACTCCAGGGAAGAGCCGAATTc                                                                                                            <  4:465590/137‑1 (MQ=255)
                                      atTCTTGTGGCCCGGCGTACGGGTTGTTTACAAAAACGCCACTGAAAACCCCGCCCCCAAGCAGGCTTAGCGGCCAGCGACCACCGCTTTTTTTTATGATCATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAat                                                                                                      <  2:650009/138‑1 (MQ=255)
                                            gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCTCGCTCGCAAGAAGTCTTAGCTGCCAGCGCCCACCGCTTTTTTTTTTGATCATTA‑ACACGACTCCAGGGACGAGCCGAATTCGTCATTATTATTGt                                                                                              >  6:298393/1‑140 (MQ=255)
                                            gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGAACTGATTACCACGCTCGCATGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATattatt                                                                                                >  3:89131/1‑138 (MQ=255)
                                             ttccccggCGTATGGTGTGTTTAAAAAAATCCAACTGATAACCACTCTCTCAAGTAGGATAAGCTGCCAGCGACCCCTGTTTTTTTTTTTTATATTTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                             <  6:236055/134‑1 (MQ=255)
                                              tgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCCGGCTTAGCTGCCAGCGACCACAGCGTTTTTTTATGATCATTA‑ACACGACTCCAGGGAGGAGCCGAATTCGGCAAGATTATTGt                                                                                              >  3:274152/1‑138 (MQ=255)
                                               gCCAGGCGTAAGCTGTGTTGAGAAAAACGGCACTGATTACACCGCTCGCAAGCAGGCTGAGCTGCCAGCGCCAACCGCGTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                             >  1:445742/1‑138 (MQ=255)
                                               gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                             >  4:140559/1‑138 (MQ=255)
                                               gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                             >  1:569289/1‑138 (MQ=255)
                                               gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                           >  5:363705/1‑140 (MQ=255)
                                               gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATAACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATAATTA‑ACACGACTCCTGGGAAGAGCCGAATTCTGCAATATTATTGTc                                                                                             >  8:146720/1‑138 (MQ=255)
                                                 cAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                           >  8:28328/1‑138 (MQ=255)
                                                 cAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                           >  3:25083/1‑138 (MQ=255)
                                                 cAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACATCCTCTTTTTTTTATGATCATTA‑ACACGACTACAGGGAAGAGCCTCATTCTGCAATATTATTGTCAt                                                                                           >  7:496164/1‑138 (MQ=255)
                                                  aGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAAGTCGGCAATATTATTGTCATTGt                                                                                        >  6:565657/1‑140 (MQ=255)
                                                  aGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTTTCATTGt                                                                                        >  6:565658/1‑140 (MQ=255)
                                                           cTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAgg                                                                                 >  8:475063/1‑138 (MQ=255)
                                                            tgtgTTGATAAAAACGCCCCTTATTCCCCCGCTCTCACGCTGGTTTAGCGCCAAGCTACAACCGTTTTTTTTTTTGATCATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGa                                                                                <  7:396541/138‑1 (MQ=255)
                                                              tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  6:610293/1‑140 (MQ=255)
                                                              tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  6:180970/1‑140 (MQ=255)
                                                              tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTATTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  6:74635/1‑140 (MQ=255)
                                                                ttGAGAAAAAAGGCACTGATTACCACTCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGAGCATTA‑ACACGCCTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  4:585277/1‑138 (MQ=255)
                                                                ttGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGGTTAGCTGCCAGCGACCACCGCTGTTTTTTATGATAATTA‑ACACGCCTCCAGGGAAGAGCCGAATTCGCCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  4:501484/1‑138 (MQ=255)
                                                                ttGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  7:420255/1‑138 (MQ=255)
                                                                ttGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  4:493307/1‑138 (MQ=255)
                                                                ttGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  3:138530/1‑138 (MQ=255)
                                                                 ttataaaaaaGGAATTTTTTACACCGCCAGCAATCTGGCTTAGCTGCCCGGGCCCACCGCTTTTTTTTATTATCATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                           <  7:137302/134‑1 (MQ=255)
                                                                 ttacaaaaaaGCCTCTTAATACCACGCTCGAACGCTGGAGTAGTTCTCAGCTACACCCTCTTTTTTTTTTGATCTTTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                           <  7:109980/134‑1 (MQ=255)
                                                                 tgattaaaaaGTCACTTTTTACCACGTTCGCATGCCGGCTTTCCTGCCAGCCACAACCTCTTTTTTTTTTGATAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                           <  2:69622/133‑1 (MQ=255)
                                                                 tcagaAAAACGGCACGTATTCCCACGCGCCCAAGCTGGCTTAGCTGCCAGACACCACCGTTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  6:519711/138‑1 (MQ=255)
                                                                 tGATAAAACAGGCACTTATTACCCCGCTCGCAAGCAGGCTTAGCGGCCAGCTACCACCTCTTTTTTTTATTATCATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                           <  4:282767/138‑1 (MQ=255)
                                                                 tGAGAAAACAGGCACTGAATCCCACGCGCGAAAGCAGGCTTAGCTGCCAGCGACCACCGTTTTTTTTTATTATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                           <  8:355861/138‑1 (MQ=255)
                                                                 tGAGAAAAAAGCCAGTTATTACAACGCTCCCAAGCAGGCTTAGCGGCCAGCGACCACCTATTTTTTTTTTTATAATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                           <  7:119964/138‑1 (MQ=255)
                                                                 tGACAAAAAAGCCACTGTTTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACGGATTTTTTTTTTCATAATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                           <  7:48453/138‑1 (MQ=255)
                                                                     aaaaaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCTGCAATATTTTTGTCATTGTATGAAGGTTATCGGGCAta                                                                     >  5:52048/1‑140 (MQ=255)
                                                                      aaaatGGCACTTATAACCAGGCTCCAACGCAGGCTTAGCTGCCAGCGACCACCGTTTTTTTTTTTTATCTTTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAt                                                                      <  8:470784/138‑1 (MQ=255)
                                                                      aaaatGGCACTGATTACCACGCTCGCACGCAGGCTTAGCGGCCAGCGACCACCGATTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAt                                                                      <  2:457906/138‑1 (MQ=255)
                                                                      aaaatGGCACTGACTACCACGCTCGCAAGCTGGATTATATGCATGCGACCACGGTTTTTTTTTAAGATAATTC‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtag                                                                    <  5:506207/140‑1 (MQ=255)
                                                                      aaaacGGCACTTATTACCCCGCTCCCAAGCAGGCTTCGCGCCCAGCTACCCCCGATTTTTTTTATTATCATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAt                                                                      <  7:536996/138‑1 (MQ=255)
                                                                      aaaacGGCACTTATAACCCCGCTCCCCACCAGGCTTAGCGGCCACCCACCACCGATTTTTTTTATTATCATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAt                                                                      <  4:99112/138‑1 (MQ=255)
                                                                           ggCACTGATAACCACGCTCGCAGGCAGTCTTACCTCCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagtag                                                                 <  4:379071/138‑1 (MQ=255)
                                                                           gccACTTATTACCACGCCCCCAAGCTGGCTTCGCTCCCAGCGACCACCGCTTTTTTTTATGATAATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagtag                                                                 <  1:247985/136‑1 (MQ=255)
                                                                           gccACTGATTCCCACGCTCGCACGCCGGCTTAGCGGCCCGCTACCACCGCTTTTTTTTATGATCATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  6:224025/138‑1 (MQ=255)
                                                                           cccACTTATTACCCCGCGCGCACGCAGGTTTAGCTGCCTCCCACCACCTATTTTTTTTAT‑TTCAATACACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagtag                                                                 <  1:132151/136‑1 (MQ=255)
                                                                            gCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGCTTTTTTTTATGATCATTA‑AAAAGACTCCAGGGAAGAGCCGCATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATTGTAGc                                                                >  1:298312/1‑138 (MQ=255)
                                                                            gCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGc                                                                >  3:261049/1‑138 (MQ=255)
                                                                                  ataaCCAGGCTCGCACCCTGGATTTGCTGCCCGCGACCACCGGTTTTTTTTATTATATTTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTa                                                          <  8:350488/135‑1 (MQ=255)
                                                                                       cACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                              <  2:34616/97‑1 (MQ=255)
                                                                                       cACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                              >  1:34616/1‑97 (MQ=255)
                                                                                         ctcTCGCAAGCTGGCTTTGCTGCCAGCGACAACCTTTTTTTTTTTTGTTTATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATATGTCATAGTAGCCCTGTATTaattt                                                   <  8:480260/136‑4 (MQ=255)
                                                                                         ctcTCCCACGCTGGCTACGCTCCCACCGACCACCGCTTTTTTTATTGATAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAata                                                   <  7:99060/136‑1 (MQ=255)
                                                                                              gCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGTTATCGGGCATAGTAGCCCTGTAATAAATATTGAc                                              >  3:255653/1‑138 (MQ=255)
                                                                                              gCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                              >  7:19315/1‑138 (MQ=255)
                                                                                              gCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGCCATAGTAGCCCTGTATTAAATATTGaa                                              >  8:546853/1‑137 (MQ=255)
                                                                                                      gCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCAGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCAc                                      >  3:311665/1‑138 (MQ=255)
                                                                                                      gCTTAGCTGCCAGCGACCACCGCTTTATTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGTATTCGGCAAGATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAATTATTGACTTTTTCAc                                      >  1:422168/1‑138 (MQ=255)
                                                                                                        ttttcTGCCAGCTACCACCGCTTTTTTTTCTTATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGAt                                  <  6:58251/136‑1 (MQ=255)
                                                                                                        ttAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCg                                    >  1:27627/1‑138 (MQ=255)
                                                                                                          cgCTGCCAGCGACCCCCTTTTTTTTTTATTATCATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGAt                                  <  1:564308/137‑1 (MQ=255)
                                                                                                          aGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGAt                                  >  8:538556/1‑138 (MQ=255)
                                                                                                                  gCGACCCCCGCTTTATTTTATGATCAATA‑ACACGACTCCAGGGAAGAGACGAATACGGCAATATTATTGTCATTGTAGGAAGGATATCTGGCATACTAGCCCTGTATACAATATTGACTTTTTCACCGATGCGTCAAg                          >  3:596450/1‑138 (MQ=255)
                                                                                                                             ttttttttttGTTAATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGCAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTg               <  1:188318/138‑1 (MQ=255)
                                                                                                                                    tatcagcATAA‑ACACGACTCCCGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGCCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa      <  6:208624/134‑1 (MQ=255)
                                                                                                                                        ctCATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg    <  8:300607/137‑1 (MQ=255)
                                                                                                                                        aTCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt  <  5:610293/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
ACGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTATGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAT  >  NZ_CP022686/2460438‑2460713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A