Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A30 F2 I0 R1 181 7914.3 8336376 98.4% 8202993 136.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,460,576 C→A 16.2% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,460,5760CA16.2% 299.1 / 9.8 162M1M (ATG→ATT) †yhjYautotransporter outer membrane beta‑barrel domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (84/45);  new base A (4/27);  total (89/73)
Fisher's exact test for biased strand distribution p-value = 1.22e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

AACGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAT  >  NZ_CP022686/2460437‑2460713
                                                                                                                                            |                                                                                                                                         
aaCGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATc                                                                                                                                           >  7:756205/1‑140 (MQ=255)
 aCGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTTTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACAACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCa                                                                                                                                          >  7:911084/1‑140 (MQ=255)
 aCGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCa                                                                                                                                          >  7:407471/1‑140 (MQ=255)
 aCGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCa                                                                                                                                          >  8:325077/1‑140 (MQ=255)
  cGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCAt                                                                                                                                         >  4:881259/1‑140 (MQ=255)
     tctGTGGAATGTCCGGGTTGCGTATCAACGGTATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCTACAACCGCTTTTTTTTA‑TGATCATTaa                                                                                                                                      >  1:651021/1‑140 (MQ=255)
       tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAaca                                                                                                                                    >  1:1073163/1‑140 (MQ=255)
       tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAaca                                                                                                                                    >  2:299511/1‑140 (MQ=255)
         tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACg                                                                                                                                  >  8:692514/1‑140 (MQ=255)
         tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACg                                                                                                                                  >  1:736563/1‑140 (MQ=255)
             aTGTCGGGGTGGCTTATAAACGATATATTATGGTTGCCAGGCGTAAGCTGTGTTGACAAAAAAGGCAATTATTACCCCGCTCGCACGCAGGCTTCGCGGCCAGCGACAACCGATTTTTTTTA‑TGAAAATTAACACGACTc                                                                                                                              <  2:1009678/140‑1 (MQ=255)
              tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTACGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCACGCAGGCTTAGCTGCCAGCGCCCCCCGCTTGTTTTTA‑TGATCATCAACAAGCCTcc                                                                                                                             >  2:683983/1‑140 (MQ=255)
              tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTcc                                                                                                                             >  7:513936/1‑140 (MQ=255)
              tGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTcc                                                                                                                             >  3:657056/1‑140 (MQ=255)
              tGTACGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCAATGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATTATTAACACtcctcc                                                                                                                             >  7:776604/1‑140 (MQ=255)
                         cGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGcc                                                                                                                  >  7:46320/1‑140 (MQ=255)
                          gTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCg                                                                                                                 >  3:813094/1‑140 (MQ=255)
                           tATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGa                                                                                                                >  4:642312/1‑140 (MQ=255)
                                 ttatatatTCTTGTTGCCAGGCTTACGCTGTGTTTACAAAAAAGGCACTTATTACCACGCGCGCAAGCTGGCTTCGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTACCACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                          <  4:241141/138‑1 (MQ=255)
                                 cGAAATATTCTTGTGCCCCGGCGTAAGGTGTGTTGACAAAAACCCCCTTTATACCCACGCGCCCAACCAGGCTTCGCTGCCCGCGACCACCGCTTTTTTTTA‑TTATCATAACCACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                          <  8:347373/140‑1 (MQ=255)
                                 agATATATTCTTGTTGCCAGGCGTAATCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACTACTCCAGGAAAGAGCCGAATTcag                                                                                                          >  5:90528/2‑138 (MQ=255)
                                 acttaaatTCTTCTCCCCTGGCTTACGGTGTGCTGATAAAACAGGCACTGATACCCCCGCGCCCAAGCCGGCTTCGCAGCCCGCCACCACCGCTTTTTTTTA‑TGATAATAACCACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                          <  2:610052/134‑1 (MQ=255)
                                   atatatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCACGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCAAGGGAAGCGCCGAATTCGGCa                                                                                                        >  8:490581/1‑140 (MQ=255)
                                    tatatTCATGTTGCCAGGCGAAAGCTGTGTTGAGAAAAAAGGCACAGATTACCACGCTCGCAAGCACTCTTAGCTGACAGAGAACACCGCTATTTTTTA‑AGATCATTAACACGACTCCATGTAAGAGCCGAATTCGGCaa                                                                                                       >  8:82538/1‑140 (MQ=255)
                                          tttgttgCCCGGCTTAAGGTGTGTTTATAAAAACGGCACTTATTACCCCGCTCGCACGCAGGCTCAGCGGCCAGCCACCACCGCTTTTTTTTT‑TGATCATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAAtattat                                                                                                 <  2:891921/139‑1 (MQ=255)
                                           ttgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATattatt                                                                                                >  2:156596/1‑140 (MQ=255)
                                            tgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTg                                                                                               >  5:121792/1‑140 (MQ=255)
                                            tgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTg                                                                                               >  1:545002/1‑140 (MQ=255)
                                             gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                              >  2:367633/1‑140 (MQ=255)
                                              ttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                             >  7:834556/1‑140 (MQ=255)
                                              tagCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATAATTAACACGCCTCCAGGGAAGAGCCGAATTCGGCAAAATTATTGta                                                                                             >  5:403606/3‑139 (MQ=255)
                                               tgCCAGGCGTACGCGGTGTTGACAAAAAAGCCACTGATTACCACGCTCGCAAGCAGGGTTAGCTGCCAGCGACCACCGTTTTTTTTTA‑TGATAATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                            <  2:545002/140‑1 (MQ=255)
                                               ggCCGGGCTTACGTTGTGTTTAGAAAAACGCCACTTATTACCACGCTCGCAAGCAGTATTAGCTGCCCGCCACCACCGCTTTTTTTTA‑TCAAAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                            <  8:236961/139‑1 (MQ=255)
                                               ggCCCGGCGTAAGTTGTGTTTATAAAAAAGGCAATTATAACCACGCTCGCAAGCTGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGAACATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                            <  3:453244/139‑1 (MQ=255)
                                               ggCCAGGCGTACGGTGTGTTTAAAAAAACGGCATTTATTACCACGCTCGCAAGCTGGATTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATAATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCa                                                                                            <  1:29520/139‑1 (MQ=255)
                                                gCCAGGCGTAAGCTGTG‑TGAGAAAAAAGGCACGGGTTACCACCCAAGCCAGCACGCATAGGAGCCAGGCCCCCCCGCATTTTTTTATTGAGAATT‑ACAAGACGACAGGGAAGCGCCCGATTAGGGAAAATTTTTTTTAtt                                                                                          >  4:541397/1‑140 (MQ=255)
                                                   aGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGt                                                                                        >  3:334036/1‑140 (MQ=255)
                                                       gTATGCTGTGTTTATAAAAACGGCCCTTATTACCAGGCTCGCACGCGGGCTTAGCTGCCAGCGACCCCCGCTTTTTTTTT‑TTAACATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGa                                                                                    <  8:756205/140‑1 (MQ=255)
                                                        tAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGaa                                                                                   >  3:20493/1‑140 (MQ=255)
                                                          cgttgtgTTGATAAAAAACGCACTGATTACCACGCTCCAAAGCAGGCTTTGCGGCCCGCCACCACCTTTTTTTTTTA‑TTATCATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAgg                                                                                 <  5:336875/137‑1 (MQ=255)
                                                             ggtgtTCACAAAAAAGGAACTTATAACCCCGCCCGCAAGCTGGCTTAGCTGCCCCCCACCACCGCTTTTTTTTA‑TTATAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGata                                                                              <  4:536278/139‑1 (MQ=255)
                                                               tgtTGATAAAAAAGTCACTTATTACCCCGCTCCCAAGATGTGTTTGGGCCCAGCTACCACGGCTTTTTTTTA‑TGATAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            <  1:121335/140‑1 (MQ=255)
                                                               tgtTGAGAAAAAAGGGACTGATTACCACGCTCGAAAGCAGGCTTAGATGCCAGCGACCACCTCTTTTTTTTA‑TGATCATTATAACGTCTCCAGGGAAGAGCGGATTTCTGCATTATTATTGTCATTGTATTAAGTatata                                                                            >  5:514982/1‑139 (MQ=255)
                                                               tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  7:8626/1‑140 (MQ=255)
                                                               tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  6:989953/1‑140 (MQ=255)
                                                               tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  1:1017372/1‑140 (MQ=255)
                                                               tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACAACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  6:521238/1‑140 (MQ=255)
                                                               tgtTGAGAAAAAAGGAACTGATTACAACGCTCGCACGCAGGATTAGCTGCAAGCGAACACCGCTTTTTTTTA‑TGATCAATAACACGACTCCAGGGAAGAGCCGATTTCGTCTATATTATTGTCATTGTATGAAGGTTATc                                                                            >  8:575115/1‑140 (MQ=255)
                                                               tggtGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAAAATTATTGTCATTGTATGAAGGATATc                                                                            >  3:672114/4‑140 (MQ=255)
                                                                gtTGAGAAAACCGCCACTGATAACCACGCTCCCACGCCGGCTTAGCTGCCAGCGACCCCCGCTTTTTTTAA‑TTATCATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                           <  6:775076/140‑1 (MQ=255)
                                                                  ttataaaaatGGCAGTGTTTCCCAGTGTCCCAAGCTGGTTTTGCTGCCAGCGACCACGGGTTTTTTTTT‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  8:16442/136‑1 (MQ=255)
                                                                  tcacaaaaaaGGCACTTATTCCCCCGCTCGCAAGCTGGCTTAGCGGCCAGCTACCACCGATTTTTTTTA‑TTATCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  6:337411/136‑1 (MQ=255)
                                                                  taacaaaaaaGCCAGTTATTCCCACGCTCCAAAGCAGGCTTTGCTGCCTGCGACCACCGATTTTTTTTA‑TCATCTTTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  2:226273/136‑1 (MQ=255)
                                                                  tGACAAAACCGGCACTGATACCCCCGCAGGCAAGCCGGCTTAGCTGCCAGCTACCCCCCTTGTTTTTTA‑TGATCATTCCCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  4:206532/140‑1 (MQ=255)
                                                                    agaAAAAATGCACTTATCACCCGCCTCGCAAGCAGGCTATGCTGCAAGCCACACCGGCTTTTTTTTA‑TGATCAATACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCa                                                                       <  4:1044879/140‑1 (MQ=255)
                                                                      aaaaaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGTAAGAGCCGATTACGTCAATATTATTGTCATTTTATGAACTATATCGGGCAta                                                                     >  6:236901/1‑140 (MQ=255)
                                                                      aaaaaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAta                                                                     >  5:99595/1‑140 (MQ=255)
                                                                       aaaaccccACTGATTACCACGCTCGCACGCAGGATTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TTATAATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtag                                                                    <  6:851815/133‑1 (MQ=255)
                                                                         aaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagta                                                                  >  1:292244/1‑140 (MQ=255)
                                                                         aaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagta                                                                  >  4:614074/1‑140 (MQ=255)
                                                                           gcgCACTTAATACCACGCCCCCAAGCAGGATTACCTGCCACCTA‑AACCGTTTTTTTTTT‑TTATAATACCCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  4:65242/138‑1 (MQ=255)
                                                                            ggCACTTATTACCACGCTCGCACGATGGCTTAGCTGCCAGCGACCACGGCTTTTTTTAT‑TGATCATACCCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  4:127059/140‑1 (MQ=255)
                                                                            ggCACTTAAAACCCCGCTCGCAAGCAGGCTTAGCTCCCAGCGACCACCGTTTTTTTTTA‑TTATCATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  1:910466/140‑1 (MQ=255)
                                                                            gccACTTATAACCACGCCCGCAAGCAGGCTTAGCTGCCAGCGACCACCTCTTTTTTTTA‑TTATAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  8:892063/138‑1 (MQ=255)
                                                                            gccACTTAATACCACGCGCCCACTCAGGCTTCGCTGCCCGCTCCCACCTCTTTTTTTTA‑TGAACATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  6:45828/138‑1 (MQ=255)
                                                                            gccACTGATAACCCCGCACGCACGCCGGCTTCGCGCCCAGCGACCACCGCTTTTTTTTA‑TGATCATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  7:588766/138‑1 (MQ=255)
                                                                            gcacatGATAACCACGCTCCCACGCGGGCTAAGCGTCCTCCGACCACCTCTTTTTTTTA‑TGATCATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  5:138299/135‑1 (MQ=255)
                                                                            gactgttaTTACCACGCTCCCAACGAGGGTTTGCGGCCAGCGACCCCCGTTTTTTTTTT‑TCTTCTTACACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  2:7457/133‑1 (MQ=255)
                                                                                cTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGt                                                           >  6:890727/1‑140 (MQ=255)
                                                                                  taTTACCACGCTCGCAAGCAGGCTTAGCTGCCTGCGACCACCGCTTTTTTTTA‑TCAAAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTAt                                                         <  6:842646/139‑1 (MQ=255)
                                                                                  gATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTAt                                                         >  4:947269/1‑140 (MQ=255)
                                                                                        cACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTAGGAAGGATATCGGGCATAGTAGCACTGTATTAAata                                                   >  6:47650/1‑140 (MQ=255)
                                                                                          cgcgcgCACGCAGGAGTAGCGTCCCGCGACCACCGTTTTTTTTTA‑TGAAAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATAtt                                                 <  3:1041951/140‑1 (MQ=255)
                                                                                           gCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGCTTTCGGCAATATTATTGTCATTGTATGAAGGATATAGGGATTAGTAGCCCTGGATTAAATTTTg                                                >  7:969330/1‑140 (MQ=255)
                                                                                           gCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTg                                                >  5:607593/1‑140 (MQ=255)
                                                                                           gCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTg                                                >  7:662652/1‑140 (MQ=255)
                                                                                             tCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGaa                                              >  2:509435/1‑139 (MQ=255)
                                                                                             tCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                              >  5:826310/1‑140 (MQ=255)
                                                                                             tCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACAAGACTCCAGGGAAGAGCAGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAGAGTAGCCCTGTATTAAATATTGaa                                              >  5:834104/1‑139 (MQ=255)
                                                                                             tCGCAAGCACGCTAAGCTGACAGCGACCACCGCTTTTTTTTA‑TGATAATTAACACGACTCCAGGGAAGAGCCCAATTCGGCAATAATATTGTCAGTGTAGGAAGGATATAGTGCATAGAAGCCCTGAAATAAATATTGAc                                              >  5:801052/1‑140 (MQ=255)
                                                                                               gCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGCCTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACtt                                            >  8:826836/1‑140 (MQ=255)
                                                                                                    cAGGCTTAGCTGCCAGCGACCACCGCTTTTATTTA‑TTATCTTTAACCACACTCCAGGTACGAGCCGAATTCGGCATTATTATTGTCTTTGAATGAAGGATATCGGGCATAGTAGCCCTGAATATAATATTGAGttattgc                                       >  2:1001438/1‑138 (MQ=255)
                                                                                                     aGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCAc                                      >  2:842755/1‑140 (MQ=255)
                                                                                                       ggttcgCTGCCCGCGACCAGGGTTTTTTTTTT‑TTATAATTCCCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCg                                    <  8:284004/135‑1 (MQ=255)
                                                                                                       gCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCg                                    >  2:467356/1‑140 (MQ=255)
                                                                                                           tgCTCCCAGCGACCACCGTTTTTTTTTT‑TTATAATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGc                                <  2:405153/139‑1 (MQ=255)
                                                                                                           aGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGc                                >  3:549236/1‑140 (MQ=255)
                                                                                                           aGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAGAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGc                                >  1:595050/1‑140 (MQ=255)
                                                                                                                 cAGCTACCCCCGCTTTTTTTTA‑GGCTCATTAACAAGACTCCAGGGAAGAGCCGAATTCGGCAAGAGTATTGTAAGGGTCTTCAGTATATAGGGCAGAGTATACCTTTAGTAAATATTTCCTTTTTCACATATTCTTCAAg                          >  4:247523/1‑140 (MQ=255)
                                                                                                                 cAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTCGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                          >  3:905498/1‑140 (MQ=255)
                                                                                                                 cAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                          >  1:405153/1‑140 (MQ=255)
                                                                                                                 cAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGGATGAAGGATATCGGGCATAGTTGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAg                          >  8:373339/1‑140 (MQ=255)
                                                                                                                          ccGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTTACTTTTTAACCGATGCGTCAAGAAAAGCGGc                 >  5:57042/1‑140 (MQ=255)
                                                                                                                              ttttttttA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCTGGCATAGTATCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGaa             >  2:968034/1‑140 (MQ=255)
                                                                                                                                     ta‑ttatCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa      <  7:251664/140‑1 (MQ=255)
                                                                                                                                      aatcaTCATACACACGACTCCAGGGCAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCTGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa      <  5:195292/136‑1 (MQ=255)
                                                                                                                                        tttccATTACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACg    <  1:776382/136‑1 (MQ=255)
                                                                                                                                          aTCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt  <  2:14119/140‑1 (MQ=255)
                                                                                                                                          aTCATAACCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt  <  8:911084/140‑1 (MQ=255)
                                                                                                                                          aTCATAAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt  <  8:269911/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                         
AACGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTTA‑TGATCATTAACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAT  >  NZ_CP022686/2460437‑2460713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A