Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A28 F35 I0 R1 174 9808.2 8488800 98.4% 8352979 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,460,576 C→A 15.4% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,460,5760CA15.4% 283.8 / 7.9 167M1M (ATG→ATT) †yhjYautotransporter outer membrane beta‑barrel domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (87/48);  new base A (2/28);  total (89/78)
Fisher's exact test for biased strand distribution p-value = 3.26e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

AACGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAT  >  NZ_CP022686/2460437‑2460713
                                                                                                                                             |                                                                                                                                          
aaCGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCAAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTATTTTT‑A‑TGATc                                                                                                                                            >  6:370908/1‑140 (MQ=255)
  cGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGCTTTTTTTT‑A‑TGATc                                                                                                                                            >  2:384746/1‑138 (MQ=255)
       tgtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGC‑TTTTTTT‑A‑TGATCATTA‑acac                                                                                                                                   >  7:7906/1‑140 (MQ=255)
       tgtcGAATGTCCGGGTTGCTTATCAACGATATATACTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCAAAGATTACAACTCTCGCAAGCAGGCTTAACTGCCAGCGACCACCGCTTTTTATT‑A‑TTATCATTA‑aca                                                                                                                                    >  5:878487/1‑140 (MQ=255)
        ttgtaaTGTCGTGGTTGCTTTTAAACTATATATTCTTTTTGCCAGGCTTAAGTGTTGTTTATAAAAAAGCCACGTATTACCACGCTCCCACGAAGGATTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATAA‑ccac                                                                                                                                   <  4:575064/136‑1 (MQ=255)
        gtgGAATGTCCGGGTTGTGTATCAACTATATTTTCTTGTTGCCCGGCGTACGGTGTGTTGATAAAAACGCCACTGATAACCACGCTCGCACGCAGGCTTAGCTGCCAGCTACCACCGCTTTTTTTT‑A‑TTATAATTA‑ccac                                                                                                                                   <  4:455645/140‑1 (MQ=255)
        gtgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ac                                                                                                                                     >  1:901709/1‑138 (MQ=255)
         tgGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑acacc                                                                                                                                  >  4:851165/1‑139 (MQ=255)
          gttaTGTCGGGGTTGCTTATCAACTATTTATTCTTGTTGCCAGGCTTAAGGTGTGTTTATAAAAAAGGCAATGATAACCCCGCTCGAAAGCAGGCTTTGCTGCCTGCGACCACCGCTTTTTTTT‑T‑TGATCATTA‑ccac                                                                                                                                   <  2:685163/135‑1 (MQ=255)
                 ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTTCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAgg                                                                                                                          >  4:1047477/1‑140 (MQ=255)
                 ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGCCTCCAgg                                                                                                                          >  6:199783/1‑140 (MQ=255)
                 ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAgg                                                                                                                          >  7:889270/1‑140 (MQ=255)
                 ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAgg                                                                                                                          >  7:657911/1‑140 (MQ=255)
                 ccGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAgg                                                                                                                          >  5:584365/1‑140 (MQ=255)
                     gTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGa                                                                                                                        >  2:314207/1‑138 (MQ=255)
                       tGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGATGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGAATCCAGGGact                                                                                                                      >  1:554945/1‑136 (MQ=255)
                         cGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGTCACTGATTACCACGCTCGCACGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGcc                                                                                                                  >  8:59099/1‑140 (MQ=255)
                           tATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGa                                                                                                                >  4:738322/1‑140 (MQ=255)
                             tCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGa                                                                                                                >  2:975500/1‑138 (MQ=255)
                              cAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGCCTCCAGGGAAGAGCCGAAtt                                                                                                             >  6:576734/1‑140 (MQ=255)
                               aaCGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAATCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAACAGCCGAATTc                                                                                                            >  3:558186/1‑140 (MQ=255)
                                 cGATATTTTCATGCGGCCCGGCGTATGGTGTGTTCATAAAACAGCCACTCATAACCCCGCCCGCAGGGCGTAATCGCGGCCAGCGACCACCGTTTTTTTAT‑T‑TGATAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                          <  4:153157/140‑1 (MQ=255)
                                  gATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACACCAGGGAAGAGCCGAATTCg                                                                                                           >  1:318050/1‑138 (MQ=255)
                                   ttatatTCTTTTTCCCAGGTTTACGCGGTTGTGACAAAACACGAACTTATTACCCCCCCCCAAACCGGTCTTAGCGCCAAGCCACCACCGGTTTTTTTT‑A‑TAAACATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                        <  6:647517/139‑1 (MQ=255)
                                   atatatTCTTGTTGCCTGCTTTTAGGTGTGTTGAAAAAAAAGGCACTGATAACCACGCTCGAACCCAGGCTAAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                        <  6:500013/140‑1 (MQ=255)
                                   atatatTCTGGTTGCCCGGAGTACGCTTGTTTTATAAAAAAGGCACTGAAAACCCCGACCCCAAGCTGGGTTTGCGGCCCGCGACCACGGGTTTTTTTT‑A‑TCATAATTA‑ACACGACTCCAGGGAAGAGCCGAATTCgg                                                                                                          <  2:265073/138‑1 (MQ=255)
                                     atatTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCa                                                                                                        >  1:612021/1‑138 (MQ=255)
                                           ttgttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGACGAATTCGGCAATattatt                                                                                                >  6:600892/1‑140 (MQ=255)
                                             gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCCCCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                              >  7:974706/1‑140 (MQ=255)
                                             gttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGACACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGt                                                                                              >  7:155573/1‑140 (MQ=255)
                                              ttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                             >  5:957772/1‑140 (MQ=255)
                                              ttgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGAGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                             >  5:958059/1‑140 (MQ=255)
                                               tgCCAGGCGTAAGCTGTGTTGAGAAAAAAGGAACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGAGACCACAGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTAGGCAATATTATTGt                                                                                              >  1:797071/1‑138 (MQ=255)
                                               tccccggCTTAAGGTGTTTTGATAAAAATGCCACTGTTACCCACCCTCGAAACATGTTTTTGCTGCCAGCTACCCCGGTTTTTTTTT‑A‑TCAACATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGTCAATATTATTGTCa                                                                                            <  8:963963/135‑1 (MQ=255)
                                                gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAATCAGGCTTAGCTGCCAGCGACCACCGCTATTTTTT‑A‑TGATAATTA‑ACACGACTCAAGGGAAGAGCCGAATTCAGCAATATTATTGACAt                                                                                           >  3:779871/1‑140 (MQ=255)
                                                gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                             >  1:317051/1‑138 (MQ=255)
                                                gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTc                                                                                             >  2:720834/1‑138 (MQ=255)
                                                gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                           >  7:112552/1‑140 (MQ=255)
                                                gCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCAt                                                                                           >  4:180745/1‑140 (MQ=255)
                                                       gTAAGCTGTGTTCTGAAAAAAGGCACTTCTTACCCCGCTCGCAAGCAGGCTTAGCTGCCCGCGACCACCGTTTTTTTTT‑A‑TGATAATAA‑CCACGACTGCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTAt                                                                                      <  1:1009615/138‑1 (MQ=255)
                                                        aaaGCTGTGGTTATAAAACAGCAACTGATAACCCCGCGCTCATGCAGGCGTATCTGCCAGCTACCCCCGATTTTTTTT‑A‑TGATAATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAGTATTATTGTCATTGTATGaa                                                                                   <  8:850691/139‑1 (MQ=255)
                                                             tgttTTGACAAAAAAGGCACTGATTACCACGCGCTCAAGCTGGCTTAGCGGCCAGCGACCCCCGCTTTTTTTT‑A‑TTATAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGata                                                                              <  4:550383/140‑1 (MQ=255)
                                                             tgtgTTTATAAAAAAGGCAATGATAACCCCGCTCCCACGCTGGCTTAGCGCCCAGCCACCCCCGGTTTTTTTT‑A‑TTATCATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGata                                                                              <  5:467452/140‑1 (MQ=255)
                                                               tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  5:619983/1‑140 (MQ=255)
                                                               tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  6:15928/1‑140 (MQ=255)
                                                               tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  8:183410/1‑140 (MQ=255)
                                                               tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  7:936749/1‑140 (MQ=255)
                                                               tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  7:244364/1‑140 (MQ=255)
                                                               tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  4:536947/1‑140 (MQ=255)
                                                               tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  3:889834/1‑140 (MQ=255)
                                                               tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTATTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCCAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  4:143321/1‑140 (MQ=255)
                                                               tgtTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCGTAGCTGCCAGCGACCACCGCGTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGACTTCGGCAATATTATTGTCATTGTATGAAGGatata                                                                            >  6:283507/1‑139 (MQ=255)
                                                                 ttGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGatata                                                                            >  2:192974/1‑137 (MQ=255)
                                                                 ttGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATc                                                                            >  2:403154/1‑138 (MQ=255)
                                                                 tagagaAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGatata                                                                            >  2:95260/3‑137 (MQ=255)
                                                                  ttataaaaatGGCACTTATTACCACGCCCGAACGCAGGTTTAGCTGCCAGCCACCACCGTTTTTTTTT‑A‑TTATAATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  5:85370/136‑1 (MQ=255)
                                                                  ttaaaaaaacGCCACTGATTCCCACGCTCTCAAGCAGGCTTAGCGCCCTGCCACCACCGTTTTTTTTT‑T‑TTATAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCg                                                                           <  1:533308/134‑1 (MQ=255)
                                                                  tGATAAAAACGCCACTTATAACCCCGCCCCCACGCAGGCTTAGCGCCCACCCACCCCCGTTTTTTTTT‑T‑TTTAAAAAC‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  3:487838/140‑1 (MQ=255)
                                                                  tGATAAAAAAGGCACTTTATACCACGCGCCCAAGCAGTGTTAGCACCCAGCGACCAGGCTTTTTTTTT‑A‑TTAAAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  5:56187/140‑1 (MQ=255)
                                                                  tGAGAAAAAAGGCACTTATTACCACGCTCGCACGCTGGTTTAGCTGCCAGCGACCACCGCTTTTTTTT‑T‑TCATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  8:657911/140‑1 (MQ=255)
                                                                  tGACAAAAACGGCCCTTATTACCCCGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGATTTTTTTT‑A‑TTATAATTC‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  4:679178/140‑1 (MQ=255)
                                                                  tGACAAAAACGCCACTGATTCCCCCGCTCCCACGACGGCTTCGCTCCCAGCGACCACCCCTTTTTTTT‑A‑TGAAAATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  4:37436/140‑1 (MQ=255)
                                                                  tGACAAAAAAGGCACTTTTAACCACGCTCCAAAGCAGGTTTAGCTGCCAGCTACCACCGATTTTTTTT‑T‑TTATCATTA‑CCACGACTCCAGGGAAGAGCCGAATTCTGCAATATTATTGTCATTGTATGAAGGatatag                                                                           <  2:751597/138‑3 (MQ=255)
                                                                  tGAAAAAAAAGGCACTTATTACCACGCTCGCAAGCAGGCTTAGCTGCCACCGACCACCGATTTTTTTT‑A‑TTATCATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCggg                                                                         <  8:261031/140‑1 (MQ=255)
                                                                    agaAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCGTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCa                                                                       >  6:733271/1‑140 (MQ=255)
                                                                    agaAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACAGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTTTATGAAGGATATCGTGCa                                                                       >  3:926443/1‑140 (MQ=255)
                                                                       aaaagGGCACTTATAACCACCCTCGCATCCCGGGTATGCTGCCAGCGACCACCGTTTTTTTTT‑A‑TGATAATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtag                                                                    <  4:778742/140‑1 (MQ=255)
                                                                       aaaacGCCACTGACGACCCCGCCCGCAACCAGGATTAGATGCCAGCTACACCCGATTTTTTTT‑A‑TTATAATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAt                                                                      <  2:59249/138‑1 (MQ=255)
                                                                         aaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagta                                                                  >  3:185228/1‑140 (MQ=255)
                                                                         aaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagta                                                                  >  5:778311/1‑140 (MQ=255)
                                                                         aaaGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtag                                                                    >  1:159951/1‑138 (MQ=255)
                                                                          aaGGCAGTGTTTCCCACGCGCGCACGCTGTCTTACCTGCCAGCGACCACCTCTTTTTTTA‑A‑TTATAATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagtag                                                                 <  6:850823/140‑1 (MQ=255)
                                                                           aGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGc                                                                >  4:443321/1‑140 (MQ=255)
                                                                            ggCATTGATTACCACGCTCGCACGCTGGCTTAGCTGCCAGCGACCCCCGTTTTTTTTT‑T‑TTATCTTAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  3:339957/140‑1 (MQ=255)
                                                                            ggCACTTATAACCCCGCCCGCACCCAGGATTAGCGCCCAGCGCCCACCGCTTTTTTTT‑T‑TTATCATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  4:723385/140‑1 (MQ=255)
                                                                            ggCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACATGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCAtagtag                                                                 <  1:620868/138‑1 (MQ=255)
                                                                            gccACTTATAACCACGCTCCCAACCTGGCTTAGCGCCCTGCCACCCCCTCTTTTTTTA‑A‑TCATCATAA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  7:198661/138‑1 (MQ=255)
                                                                            cgaccttaTTACCACGCTCGCACCGTGGAGTAGCTTCCCGCGACCACCGTTTTTTTTT‑T‑TG‑TTATTACCCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  7:536418/133‑1 (MQ=255)
                                                                            cgCACTGAATACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCCCCGCTATTTTTT‑A‑TGATCATTA‑ACACCACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  7:90891/139‑1 (MQ=255)
                                                                             ccccTGATTCCCCCGCGCCCAAGCCGGCTTAGCTGCCCGCGACCCCCGATTTTTTTTAA‑TTATCATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  8:33497/137‑1 (MQ=255)
                                                                             ccacgttttACCCGGCTCCCCAGCTGGCTTCGCTCCCCGCGACCACCGTTTTTTTTT‑A‑TTATAATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGccc                                                              <  3:651589/133‑1 (MQ=255)
                                                                              cACTGATTCCCCCGCTCGCACGCAGGCTTCGAGGCCAGCGACCCCCGCTTTTTTTT‑A‑TCATCATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGcc                                                               <  1:1064325/138‑1 (MQ=255)
                                                                                          cGCCCGCAAGCAGGTTAAGGCGCCACCCACCACCTTTTTTTTTT‑C‑TTATCATAC‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATAtt                                                 <  3:204849/140‑1 (MQ=255)
                                                                                             tCGCAAGCAGGCTTAGCTGCCAGCGCCCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                              >  7:436588/1‑140 (MQ=255)
                                                                                             tCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                              >  7:364957/1‑140 (MQ=255)
                                                                                             tCGCAAGCAGGCTTAGATGCCATCGAACCCCGCTTTTTTTT‑A‑TGATCATTA‑AAACGCCTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGACGGATATCGGGCATTGTAGCCGTGTATTAATTATTGag                                              >  8:802706/1‑139 (MQ=255)
                                                                                              cGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGa                                               >  1:967032/1‑138 (MQ=255)
                                                                                               gCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGAc                                              >  1:349180/1‑138 (MQ=255)
                                                                                                 aaGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACtt                                            >  2:416225/1‑138 (MQ=255)
                                                                                                     aGGCTTATCTGCCAGCGACAACCGCTTTTTTTT‑A‑TGATCATTA‑AAAATACTCCAGGGAAGAGCCGAATTCGGCAATATTTTTTTCATTGTATGAAGGATATCGGGAATAGTAGCCCGGTATTAAATATTGACTTTTTCAc                                      >  6:326205/1‑140 (MQ=255)
                                                                                                         ttcgCTGCCACGGA‑CACCCCTTTTTTTT‑ATTTATCATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCg                                    <  2:338393/135‑1 (MQ=255)
                                                                                                         ttAGGTGCCAGCTACCACCGCTTTTTTTT‑A‑TGACAATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGAt                                  <  4:983833/140‑1 (MQ=255)
                                                                                                           aGCTGCCAGCGCCCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGAt                                  >  1:371081/1‑138 (MQ=255)
                                                                                                                      accaccGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAg                     >  3:544059/1‑140 (MQ=255)
                                                                                                                                     t‑a‑ttatAATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTAATTGTATGATGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAAtttt        <  2:873309/138‑1 (MQ=255)
                                                                                                                                         tGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGTGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTa      <  2:104113/138‑1 (MQ=255)
                                                                                                                                           ttCATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGTCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt  <  6:732195/139‑1 (MQ=255)
                                                                                                                                           aacATTA‑CCACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt  <  3:1064690/138‑1 (MQ=255)
                                                                                                                                           aTCATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt  <  5:800180/140‑1 (MQ=255)
                                                                                                                                           aTCATAA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAt  <  4:146154/140‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                          
AACGCTCTGTGGAATGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACCACGCTCGCAAGCAGGCTTAGCTGCCAGCGACCACCGCTTTTTTTT‑A‑TGATCATTA‑ACACGACTCCAGGGAAGAGCCGAATTCGGCAATATTATTGTCATTGTATGAAGGATATCGGGCATAGTAGCCCTGTATTAAATATTGACTTTTTCACCGATGCGTCAAGAAAAGCGGCTGAAATTTTTACGAT  >  NZ_CP022686/2460437‑2460713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A