Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A27 F2 I0 R1 73 6005.1 5484070 98.3% 5390840 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,572,721 A→C 15.1% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,572,7210AC15.1% 236.0 / 10.8 144F186C (TTT→TGT) CIW80_RS12885ROK family protein
Reads supporting (aligned to +/- strand):  ref base A (66/53);  new base C (3/22);  total (69/75)
Fisher's exact test for biased strand distribution p-value = 5.35e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

AGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGTT  >  NZ_CP022686/2572586‑2572850
                                                                                                                                       |                                                                                                                                 
tgCTGTTGGCGAAAACGTGCCACCTGTTGCATGCCACAAGCATCACCACGTTGGGCCTGGGTGACAATCGCCGCATATGGTAGAATGTTACCTGTCATCTGTTTATAGGGTTCACTAAAACCGCTGCCGGAAACAAACGa                                                                                                                               <  2:21301/139‑1 (MQ=255)
     ttGGCGAAAACGTGCCACCTGCTGCATGGCTCAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGCGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAACCCGCTGCCGGAACCAAACGACTCCa                                                                                                                          >  5:571318/1‑140 (MQ=255)
     tgggCTAAACCGTGCCACCTGCGGCATGGCACAAGCATCACCCCGTGGGGCCAGGGTGACAATCGCCGCATACGTGAGCAAGTTACCAGTCATCTGTTGATAGCTTTCACTAAAACCGCTGCCGGAAACAAACGACTc                                                                                                                            <  8:455241/136‑1 (MQ=255)
     tgggCGAAAACGTGCCACCTGCTGCATGGCACAAGAATCACCACGTTGGGCCTGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGGCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAAAAAACGACTc                                                                                                                            <  8:435521/136‑1 (MQ=255)
       ggCTAAAAGGTGCCACCTGCTGCTTGGCAATAGCATCAACTCGTTGTGCCAGGGTGACATTGCCCGCTTAAGTGAGAATGTGACCTGTCTTGTTTTGATAGCTTTCAATAAAACCCGTGCCGGAAACACACGACTCCACg                                                                                                                        <  2:239505/140‑1 (MQ=255)
       ggCGATAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACAGCTGCCGGAAACAAACGACTCCACg                                                                                                                        >  4:314948/1‑140 (MQ=255)
       gcctaaacCGGGCCCCCTGCTGCTGGGCACAAGCACCCCCACGTTGTGCCTGGGTTAAAATCGCCGCAGAAGTGACCAAGTTACCAGCCATCTGTTGATAGCGTCCACTAAAACCGCTGCCGGAAAAACACGACTCCa                                                                                                                          <  8:62274/134‑1 (MQ=255)
                         gctgcATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCaa                                                                                                      >  5:157676/1‑140 (MQ=255)
                            gcATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAg                                                                                                   >  5:640489/1‑140 (MQ=255)
                                   acaAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAact                                                                                              >  8:206529/1‑138 (MQ=255)
                                    caAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAactgac                                                                                           >  2:342511/1‑140 (MQ=255)
                                     aaGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAactgact                                                                                          >  6:362744/1‑140 (MQ=255)
                                       gCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCa                                                                                                                          <  4:53405/106‑1 (MQ=255)
                                       gCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCa                                                                                                                          >  3:53405/1‑106 (MQ=255)
                                        cATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGAATCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGAt                                                                                       >  2:35698/1‑140 (MQ=255)
                                           caccagGTTGTGCCTGGGTGACATGCGCCGCATAAGTCAGCAATTTACCAGTAATCTGTTTATTGCGTTCACAAAACCCGCGGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGa                                                                                    <  5:488532/140‑1 (MQ=255)
                                            cccacTTGGTGCCTGGGTGACAATCGGCGGTGATTGGTGCTTGTTACCTTTTTTTTGTTGATAGCGTTCCATAAACCGGATGCGGGATACACTCGACTCCACGCAGTTGTGTTTGCCGCAATTGCAACTGACTGATGGAc                                                                                   <  2:661518/139‑1 (MQ=255)
                                            accagGTTGTCCCTGGGGTACAACCGCCCCAGAAGTGAGCAAGTAACCCGTCATGTGTTTATCGCGTACAATAAAACCGCGCCCGCAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                   <  6:133105/140‑1 (MQ=255)
                                            accacGTTGTGCCTGGGTGACAAGCGCCGCAGAAGTGAGCAAGTTCCCAGTCATCTGTTGATCGCGTTCACTAAACCCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                   <  1:5890/140‑1 (MQ=255)
                                            accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                   >  3:681185/1‑140 (MQ=255)
                                            accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                   >  5:53181/1‑140 (MQ=255)
                                            accacGTTGTGACAGGGTGACAATCGCCCCAGAAGTGAGCAAGTTACCAGTCATATGTTGATAGCGTTCACAAAAACCGCTGACGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACAGATGGAc                                                                                   >  2:526862/1‑140 (MQ=255)
                                            accaaattGTTCCAGGGTGACAATCGCAGCAGAAGTGAGCAAGTTACCAATCATCTATAGAAAGAGTTCACTAAAAACGCTGCCGGCAACCAACGACTCAACGCAGATGAGTTTGCCGCAAAAGAAACTTACTTATGGAc                                                                                   >  4:664351/7‑140 (MQ=255)
                                            acaccGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCATGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGCAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATgg                                                                                     <  8:60670/134‑1 (MQ=255)
                                              cacTTTGGGCCAGGGTCAAAATCCCCGAAGATGTGAGCAAGTTACCCTTCATCTGTTGATAGCGTTCACAAAACCCGCTGCCGCAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCa                                                                                 <  6:485077/140‑1 (MQ=255)
                                                   tgtgCCCGGGTGACAATCGCCGCAGACGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCGGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCt                                                                              <  8:582332/138‑1 (MQ=255)
                                                   tgtgCCAGGGTGACAATCGCCGCAGATGTGCGCATGTTACCAGTCATCTGTTGAGGGCGTTCACAAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCt                                                                              <  8:348737/138‑1 (MQ=255)
                                                   tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAATTTACCAGTCATCTGTTGATAGCGTTCCCTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGCCCATCTTc                                                                            >  1:30941/1‑140 (MQ=255)
                                                   tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                            >  6:540815/1‑140 (MQ=255)
                                                   tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                            <  4:426008/140‑1 (MQ=255)
                                                   ggtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCAATAAAACCGCTGCCGCAAACACCCGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                            <  6:596023/139‑1 (MQ=255)
                                                     ggCCAGGGGGACACTCGCCGCAGAAGTGAGCAAGTTACCCGGCATATGTTGATAGCGTCCACTAAACCCGCTGCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCt                                                                          <  6:622124/139‑1 (MQ=255)
                                                      gCCAGGGTGACAATGGCCCCATAAGTGAGCAAGTTACCTGGAATCTGTTTATAGCATTTAGTAAAACCGCTGCCTTAAACAAAAGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         <  6:318550/140‑1 (MQ=255)
                                                      gCCAGGGTGACAATCGCCGCATAATTTAGAAATTTACCCGTCATCTGTTGATAGCGTTCACAAAAACCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTcc                                                                           <  8:324740/138‑1 (MQ=255)
                                                      gCCAGGGTGACAATCGCCGCAGAATTCAGCATGTTACCAGGCATCTGTTGATAGCGTTCACTAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         <  3:63926/140‑1 (MQ=255)
                                                      gCCAGGGTGACAATCGCCGCAGAAGTGCGCAAGTTACCAGTCATCTGTTTATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         <  1:517764/140‑1 (MQ=255)
                                                      ccctgggTGACAATCGCCGCCGAAGTCAGAACGTTACCAGTCATCTGTTTATAGCTTACACTAAACCCGCGGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTcc                                                                           <  7:38296/134‑1 (MQ=255)
                                                      cccAGGGGCAAAATCGCCGCACAAGTTACCAAGTTACCAGGAATATGTTTATCGCGTTCAATAAACCCGCGGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         <  2:709033/139‑1 (MQ=255)
                                                       cctgggTGACAATCGCCGAATAAGTGAGAAAGTTACCAGTCATTTGTTGATAGCTTGCACTAAAACCGCTGCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCt                                                                          <  8:565363/135‑1 (MQ=255)
                                                       ccAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTAGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCt                                                                          >  7:196790/1‑138 (MQ=255)
                                                        ctgggTTACAATCGCCGCAGATGTTAGCAAGTTCCCAGTCATCTGTTGATCGCGTTCAAAAAACCCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                       <  1:159013/138‑1 (MQ=255)
                                                        ctgggTGACAATCGCCGCAGATGTGACAATGTTACCAGTCATCTGTTTATAGCGTTCACTAAACCCGCGGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                       <  3:677749/138‑1 (MQ=255)
                                                        cAGGGTGACAAGCGCCGCAGATGTGAGCAATTTACCAGTCATATGTTGATCGCGTGCACTAAAACCGCTGCCGCAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         <  7:602532/138‑1 (MQ=255)
                                                        aaGGGTTACAATCGCCCAACAAGTGAGCAATTAACCAGTCATCTGTTTATAGCGTACACAAAAACCGGTGCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         <  7:141245/137‑1 (MQ=255)
                                                           ggTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                                    >  3:110292/1‑140 (MQ=255)
                                                           ggTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCAGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                                    >  4:102354/1‑140 (MQ=255)
                                                             ttaCAATCCCCGAAGCCTTGAGCACCTTACCAGTCATCTGTTGTTCGCGTTCCCTAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGta                                                                  <  4:143760/138‑1 (MQ=255)
                                                             ttaCAAGCGCCGCACAAGTGACCAAGTTACATGTCATCTGTTGAAAGCGTTCACTAAAACCGGTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGta                                                                  <  6:608184/138‑1 (MQ=255)
                                                             tGACTATCGCCGCATAAGTGAGCAAGTTACCAGTCAGCTGTTGATAGCGTTCAAAAAAACCGCGGCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGta                                                                  <  3:84103/140‑1 (MQ=255)
                                                             gtaCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTAACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                                    <  8:216027/136‑1 (MQ=255)
                                                             gtaCAATCGCCGCAGAAGTGAGAAAGTTACCCTTCTTCTGTTGATAGCGAGCAATAAAACCGCGGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                                    <  7:264441/136‑1 (MQ=255)
                                                               aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                                <  6:157676/140‑1 (MQ=255)
                                                                     gccgcATAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTgg                                                          >  6:525730/1‑140 (MQ=255)
                                                                      ccgcAGAAGTTAGCAAGTTACCAGTCATCTGTAGATCGCGGTCACTAAACCCGCGGCCGCAAAAAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGc                                                         <  3:353734/140‑1 (MQ=255)
                                                                      ccgcAGAAGTGAGCAAGTAACCTGTCATCTGTTTATTGCGTTAACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGc                                                         <  5:461983/140‑1 (MQ=255)
                                                                       cgcAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCTGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTgg                                                          >  8:169971/1‑138 (MQ=255)
                                                                           gAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATATTCCTTCTCCGTATAGCCTGGCAAc                                                      >  8:536944/1‑138 (MQ=255)
                                                                                 aGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGata                                                >  8:395540/1‑138 (MQ=255)
                                                                                 aGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATg                                              >  5:414944/1‑140 (MQ=255)
                                                                                 aGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATg                                              >  6:575927/1‑140 (MQ=255)
                                                                                 aGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGGATAGCCGTGCAAAGTGAAATg                                              >  4:461830/1‑140 (MQ=255)
                                                                                 aGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACACTCTTCCTGCTCCTGATAGCCTGGCAACGTGATATg                                              >  1:313714/1‑140 (MQ=255)
                                                                                  gacaGTAACCAGTCATCTTTTGATAGGGTGCACTAAACCCGCGGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGa                                             <  3:358003/137‑1 (MQ=255)
                                                                                  ccAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGatat                                               <  7:38352/137‑1 (MQ=255)
                                                                                   cAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATg                                              >  8:474247/1‑138 (MQ=255)
                                                                                   cAAGTTACCAGTCATCTGTAGCAAGCGTTCACTAAAACCGCTGCCGTAAACACACGACTCCACGCAGTAGACTATGCCGCAATAGCAAATGACAGAGGGTCCATCTAACTGCTCAGTCTAGCCTGGCAACGGGATATg                                              >  7:499558/1‑138 (MQ=255)
                                                                                   cAAGTTACCAGTCAACTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTTTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTGCCTGCTCCGTATAGCCTGGCAACGTGATATg                                              >  7:274925/1‑138 (MQ=255)
                                                                                     aGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACAGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGAATGATGGACCATCTTCCTGCTACGTATAGCCTGGCAACGTGATATGACCg                                          >  1:470820/1‑140 (MQ=255)
                                                                                            attCATCGGTTGATCGCGTCCAATAAAACCGCTGCGGTAACCAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTc                                     <  8:34920/136‑1 (MQ=255)
                                                                                                 tCTGTTGATTGCGTCCAATAATACCGCTGCCGGAAACACACGACTCCACGCAGGTGTGTTTGCCGCTCTAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgcagca                              <  2:177710/140‑1 (MQ=255)
                                                                                                  cTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgcagc                               >  8:500455/1‑138 (MQ=255)
                                                                                                  cTGTTCATAGCGTGCACTAAACCCGCTCCCGCAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCAt                             <  1:574601/140‑1 (MQ=255)
                                                                                                       gATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTc                          >  8:180340/1‑138 (MQ=255)
                                                                                                           gCGTTCACTAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                    <  1:152312/140‑1 (MQ=255)
                                                                                                           gCGTTCACTAAAACGGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                    <  3:297773/140‑1 (MQ=255)
                                                                                                           gCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                    <  5:540815/140‑1 (MQ=255)
                                                                                                           gCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                    <  5:362744/140‑1 (MQ=255)
                                                                                                           gCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                    >  3:419752/1‑140 (MQ=255)
                                                                                                           gCGTTCACTAAAACCGCGGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                    <  1:696619/140‑1 (MQ=255)
                                                                                                           cggctCACTAAACCCGCTGCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                    <  5:183787/136‑1 (MQ=255)
                                                                                                            cGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCt                                                                              >  7:543984/1‑81 (MQ=255)
                                                                                                            cGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCt                                                                              <  8:543984/81‑1 (MQ=255)
                                                                                                            cGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGc                   >  3:116958/1‑140 (MQ=255)
                                                                                                            cGTACACTAAAACCGCTGCCGGAAACAAACGACTCCACGCACTTGTCTATGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTACGAATAGCCTGCCAACGTGATATGACCGCATTCGGCACCATTTCCCCAGGc                   >  5:46169/1‑140 (MQ=255)
                                                                                                               tCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGcccc                >  3:594515/1‑140 (MQ=255)
                                                                                                               tCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGcccc                >  2:15907/1‑140 (MQ=255)
                                                                                                                cACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATAGACCATCTTCCTTCTCCGTATAGCCTTGCAACGTGATATGACCGCAATCGGCAGCCTTTCCCCAGGccccg               >  4:127499/1‑139 (MQ=255)
                                                                                                                   tAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         >  3:446072/1‑79 (MQ=255)
                                                                                                                     aaaGCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATaaa          <  4:681185/140‑1 (MQ=255)
                                                                                                                      aaCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAAc         >  2:11190/1‑140 (MQ=255)
                                                                                                                           gtgctgtaaaaaaacGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg    <  6:414944/133‑1 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         >  6:606112/1‑71 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         <  5:606112/71‑1 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCaa                                                       >  5:558075/1‑89 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg    >  3:270760/1‑140 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg    >  1:21031/1‑140 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGATCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg    >  2:192069/1‑140 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCAGGTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg    >  4:571806/1‑140 (MQ=255)
                                                                                                                           cTGCCGCAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg    <  6:387612/140‑1 (MQ=255)
                                                                                                                               cGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGtt  >  8:262411/1‑138 (MQ=255)
                                                                                                                                       |                                                                                                                                 
AGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGTT  >  NZ_CP022686/2572586‑2572850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A