Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A29 F36 I0 R1 179 9550.8 8038540 98.3% 7901884 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,572,721 A→C 14.8% F186C (TTT→TGT)  CIW80_RS12885 ← ROK family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,572,7210AC14.8% 330.6 / 20.2 193F186C (TTT→TGT) CIW80_RS12885ROK family protein
Reads supporting (aligned to +/- strand):  ref base A (96/64);  new base C (2/31);  total (98/95)
Fisher's exact test for biased strand distribution p-value = 3.31e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

AGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGTT  >  NZ_CP022686/2572586‑2572850
                                                                                                                                       |                                                                                                                                 
     ttGGCGAAACCGGGCCACCTGCTGCAGGGCACAAGCATCACCACGTGGTGCCTGGGTGACAAGCGCCGCATAAGTGAGCATGTTACCCGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACACACGACTCCa                                                                                                                          <  4:487684/140‑1 (MQ=255)
       ggCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCAcc                                                                                                                        >  4:274520/1‑139 (MQ=255)
                  gCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGCGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGttt                                                                                                             >  6:209336/1‑140 (MQ=255)
                          ctgcATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAAt                                                                                                     >  1:407111/1‑140 (MQ=255)
                             cATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGc                                                                                                  >  7:203113/1‑140 (MQ=255)
                               tGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCaa                                                                                                >  2:926943/1‑140 (MQ=255)
                                 gaaaaaGCATCACCACGTTGTGCCTGGGTGAAAATCGCCGCAGATGTGCGAAAGTTACCAGTCATCTGTTTATTGCGTTCAATAAACCCGCTGCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAact                                                                                              <  3:274520/136‑1 (MQ=255)
                                 gCACAAGCACCACCACGTGGTGCCTGGGTTCAAAGCGCCGCAGAAGTGCGCAAGTAACCCGGCATCTTTTGATAGCGTGCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAact                                                                                              <  8:206296/140‑1 (MQ=255)
                                 gCACAAGAATCCCCACTTTGTGCCTGGGTGACAATCCCCGCATAAGGGAGCATGTAACCAGTCATCTGTTTTTCGCGTTCACTAAAACCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAact                                                                                              <  1:936762/140‑1 (MQ=255)
                                 ctgtatgCATCCCCAGTTTGGGCCTGGGTGAAAATGGCCGCCTAAGTTACAAAGTTACCTGTATTTTTTTTATAGCGTCCAATAAAACCGCTGCCGTAAAAAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAact                                                                                              <  2:520735/134‑1 (MQ=255)
                                  cacaAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAactg                                                                                             >  5:813401/1‑140 (MQ=255)
                                  cacaAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAactg                                                                                             >  1:529962/1‑140 (MQ=255)
                                  cacaAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTAATCTGTTGATAGCTTTCACTAAAACCGCTGCCTGAAACAAACGACTCCACGCAGTTTTGTTTGACGCAATAGCAactt                                                                                             >  4:397837/1‑139 (MQ=255)
                                   aaaaGAATCACCACGTTGAGCCAGGGTGACAATCGCCGCAGAAGTGAGAAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAactga                                                                                            <  2:811707/138‑1 (MQ=255)
                                       gCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAActgactga                                                                                        >  3:610775/1‑140 (MQ=255)
                                       gCATCACAACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAActgactga                                                                                        >  7:668479/1‑140 (MQ=255)
                                         accaccagGTTGTGCCTGGGTGACAATCGCCGCATAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCGGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATg                                                                                      <  6:732353/138‑1 (MQ=255)
                                         aTCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATg                                                                                      <  8:559266/140‑1 (MQ=255)
                                           caccacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGATCCAAACGACTCCACGCAGTTGTGTTTGCCGCACTAGCAACTGACTGATggc                                                                                    >  5:588631/1‑139 (MQ=255)
                                            accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATATCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                   >  1:695841/1‑140 (MQ=255)
                                            accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                   >  1:1004040/1‑140 (MQ=255)
                                            accacGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGAc                                                                                   >  4:96889/1‑140 (MQ=255)
                                              cccGTTGGCCCAGGGTCCCAAACGCCGCAGAATGGAGCATGTTCCCAGGCTTATGTTTAACGCGTTACCTAAAACCGCTCCCGCAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCa                                                                                 <  8:108303/138‑1 (MQ=255)
                                                  ttGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCtt                                                                             >  1:365365/1‑140 (MQ=255)
                                                   tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                            >  1:344661/1‑140 (MQ=255)
                                                   tgtgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                            <  4:110271/140‑1 (MQ=255)
                                                   tgtcCCCGGGTGACAAGCGCCGAAGAAGTGAGCATTTAACCAGCCATCTGTTGATAGCGTCCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTATGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                            <  1:842005/140‑1 (MQ=255)
                                                   ggggCCTGGGTGACAATCGCGCCATAAGTGAGCAAGTAACCAGTCATCTGTTTATTGCGTTAACTAAAACCGCTGCCGAAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTc                                                                            <  1:755170/137‑1 (MQ=255)
                                                     tgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCt                                                                          >  3:141994/1‑140 (MQ=255)
                                                     tgCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCt                                                                          >  1:428179/1‑140 (MQ=255)
                                                      gCCTGGGTGACATGGGCCGCAGAAGTGAGCAAGTAACCAGTCATCTGTTTATAGCGTTCACTAAACCCGCTGCGGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         <  6:22825/140‑1 (MQ=255)
                                                      gCCTGGGGGACACTCGCCGGAGAAGTGAGCAAGTTACCAGTCTTCTGTTGATAGCGTTCTCTAAAACCGCTGCCGCAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         <  2:118212/140‑1 (MQ=255)
                                                      gCCCGGGTGACAATCGCCGAAGAAGTGAGCAAGTTACCAGTAATCTGTTGATAGCGTTCAATAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         <  1:649609/140‑1 (MQ=255)
                                                      gCCAGGTAGACAATCGCCGCAGAAGTGAGAAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         <  4:564632/140‑1 (MQ=255)
                                                      gCCAGGGTGAAATTCGCCGCAGACGTTAGCAAGTTACCAGTCATCGGTTGATCGCGTACACTAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTg                                                                         <  4:613942/140‑1 (MQ=255)
                                                        ctgggTTACAATAGCCGCATATGTGAGAATGTTACCTGTCAACTTTTGATAGCGTTAACTAAAACCTCTGCCGCAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                       <  2:893368/138‑1 (MQ=255)
                                                        cAGGGTGACAATCGCCGCCGAAGTTAGCAAGTTACCAGTCATCTGTTGATAGCGTACCCTAAAACCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                       <  4:770788/140‑1 (MQ=255)
                                                        cAGGGTGACAATCGCCGCATAAGTGAGCAAGTTCCCAGTCATCTGTTGATAGCGTTCACTAAACCCGCGGCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                       <  1:576349/140‑1 (MQ=255)
                                                        aaGGGTCAAAAGCGCCGCAGATGTGCGCAAGTTCCCTGTCATCTGTGGATAGCGTTCACTAAACCCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                       <  6:376725/139‑1 (MQ=255)
                                                           ggTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCg                                                                    >  1:151744/1‑140 (MQ=255)
                                                           ggTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATATGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAAAGACTCCACGCAGTTGTGTTTGCCGCAAATGCAACTCACTGATCGACCATCAACCTGCTCCg                                                                    >  4:169698/1‑140 (MQ=255)
                                                             ttaCAATCGCCGCATACTTTAGCATGGTACCTGTCATCTGTTGAATGGTTACTCTAAAACCGCTCCGGCAACCACTCGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGta                                                                  <  2:15128/138‑1 (MQ=255)
                                                             ttaCAATCGCCGCATAAGTGAGCAAGTACCAAGTCATGTGTTGATAGGGTACATAAAAACCGCTGCCGAAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGta                                                                  <  5:209336/138‑1 (MQ=255)
                                                             tgaaatgcgccgcATAAGTTAGCATTTTCCCCTTCATGTGTTTATCGCGTACACTAAAACCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGta                                                                  <  3:697610/133‑1 (MQ=255)
                                                             tGACAGTCGCCGCAGACGGTCGCACTTTCCCCGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGta                                                                  <  6:60137/140‑1 (MQ=255)
                                                             tGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGta                                                                  <  3:471624/140‑1 (MQ=255)
                                                             ccccactCGCCGCAGATGCCCGCAAGTTACCAGTCAGCTGTTTATACCGGTCACTAAAACCGCGGCGGGAAACACACGCCTCCACGCCGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGta                                                                  <  7:481420/134‑1 (MQ=255)
                                                               aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                                >  8:631684/1‑140 (MQ=255)
                                                               aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                                >  5:71263/1‑140 (MQ=255)
                                                               aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                                >  7:638501/1‑140 (MQ=255)
                                                               aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtata                                                                >  4:925223/1‑140 (MQ=255)
                                                               aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGtaga                                                                >  1:912293/1‑138 (MQ=255)
                                                               aCAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGATGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACAGATCGCCCATCATCCTGCTCCGtata                                                                >  8:292357/1‑140 (MQ=255)
                                                               aCAATCGCCGCAGAACTGAGCAAGTTACCAGTCATCTGTTGATAGCGATCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTAGTGTTAGCCGCAATAGCACCTGACTGATGGACCATATTCCTGCTCCGtata                                                                >  2:705812/1‑140 (MQ=255)
                                                                 aaTCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGc                                                              >  8:448323/1‑140 (MQ=255)
                                                                     gccgcAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTgg                                                          >  6:780722/1‑140 (MQ=255)
                                                                     gccgcAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACAATCTTCCTGCTCCGTATAGCCTgg                                                          >  1:773678/1‑140 (MQ=255)
                                                                        ccAGAAGTCACCAATTTCCCAGTCATCTGTTGATAGCGTTCACTAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCaa                                                       <  1:913661/139‑1 (MQ=255)
                                                                          aGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAACCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACg                                                     <  4:681244/140‑1 (MQ=255)
                                                                                 aGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATg                                              >  6:90481/1‑140 (MQ=255)
                                                                                 aGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATg                                              >  7:839346/1‑140 (MQ=255)
                                                                                 aGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATg                                              >  6:692419/1‑140 (MQ=255)
                                                                                 aGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATg                                              >  1:199459/1‑140 (MQ=255)
                                                                                 aGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATg                                              >  6:185648/1‑140 (MQ=255)
                                                                                       taaCCCGTCATCTGTTGATAGCGTTCACTAAACCCGCTCCCGGAAACAAACGACTCCACGCAGGTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCa                                        <  3:547047/138‑1 (MQ=255)
                                                                                            cggcATCTGTTGATAGCGTGCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCgg                                   <  3:778180/137‑1 (MQ=255)
                                                                                             gccATCTGTTGATAGCGTTCACTAAACCCGCTGCCGGAACCAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgc                                  <  2:467532/138‑1 (MQ=255)
                                                                                             gTCTTCTGTTGATTGCGTTCACTAAACCCGCGGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgc                                  <  6:827024/140‑1 (MQ=255)
                                                                                                 tCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCTGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCGGGCAACGTGATATGACCGCATTCGgcagca                              >  7:234072/1‑140 (MQ=255)
                                                                                                 ccTGTTGATAGCCGTCCCTAACCCCGCTGCCGGAACCACACGCCGCCACTCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGgcagca                              <  3:922626/139‑1 (MQ=255)
                                                                                                  gtGTTTATAGGGATCACAAAACCCGCTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCAt                             <  5:914358/139‑1 (MQ=255)
                                                                                                  cTGTTGATCCCGTTCACTAAACCCGCGGCCGTAACCACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCAt                             <  5:410452/140‑1 (MQ=255)
                                                                                                     ttGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTc                          >  5:801811/1‑140 (MQ=255)
                                                                                                     ttGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTATGCCGCAATAGCAACTGACTGATGGACAATCTTCCTGCTCCGTATAGCCTGGCAACTTGATATGACCGCATTCGGCAGCATTTc                          >  2:333645/1‑140 (MQ=255)
                                                                                                        aTAGCGTTCACTTAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTcccc                       >  3:391110/1‑140 (MQ=255)
                                                                                                        aTAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTcccc                       >  8:350425/1‑140 (MQ=255)
                                                                                                        aTAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCAACGCAGTTGTCTTTGCCGCAATAGCAACTGACAGATGGACCATCTTCCTGCTCCGGATAGCATGGCAACGTGATATGACCTCATTCGGCAGCAtttaacc                       >  5:149579/1‑136 (MQ=255)
                                                                                                          aGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAg                     >  2:212970/1‑140 (MQ=255)
                                                                                                           gCGTTCACTAAAACCGGTGCCGTAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGAAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                    <  6:808938/140‑1 (MQ=255)
                                                                                                           gCGTTCACTAAAACCGCTGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                    <  7:102093/140‑1 (MQ=255)
                                                                                                           gCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                    <  4:201845/140‑1 (MQ=255)
                                                                                                           gCGTTCACTAAAACCGCTGCCGGAAAAAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAgg                    <  7:908825/140‑1 (MQ=255)
                                                                                                            cGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGc                   >  3:585255/1‑140 (MQ=255)
                                                                                                            cGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACAATCATCCTGCTACGTATAGCCTGGCAACGTGATATGACCCCGTTCGGCAGCATTACCCCAGGc                   >  1:273022/1‑140 (MQ=255)
                                                                                                               tCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACAGCATTCGGCAGCATTTCCCCAGGcccc                >  6:650080/1‑140 (MQ=255)
                                                                                                               tCACTAAAACCGCTGCCGCAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGcccc                <  6:465117/140‑1 (MQ=255)
                                                                                                                     aaaCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATGTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATaaa          <  4:393077/140‑1 (MQ=255)
                                                                                                                     aaaCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATaaa          <  2:662359/140‑1 (MQ=255)
                                                                                                                      aaCCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGCCCGCATTCGGCAGCATTTCCCCAGGACCCTATAAAc         >  7:569252/1‑140 (MQ=255)
                                                                                                                        ccGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGCCCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACgg       >  4:235981/1‑140 (MQ=255)
                                                                                                                         cgcgGCCGTAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGt      <  8:681505/140‑1 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCNGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGGATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg    >  1:833932/1‑140 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCCTCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg    >  8:677142/1‑140 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCt                                                                       <  7:359708/73‑1 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg    >  4:255138/1‑140 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg    >  5:152497/1‑140 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg    >  2:205919/1‑140 (MQ=255)
                                                                                                                           cTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCg    <  6:152497/140‑1 (MQ=255)
                                                                                                                            tGCCGGAAACACACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGt   <  4:887378/140‑1 (MQ=255)
                                                                                                                            tGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGt   <  7:680436/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                 
AGCTGTTGGCGAAAACGTGCCACCTGCTGCATGGCACAAGCATCACCACGTTGTGCCAGGGTGACAATCGCCGCAGAAGTGAGCAAGTTACCAGTCATCTGTTGATAGCGTTCACTAAAACCGCTGCCGGAAACAAACGACTCCACGCAGTTGTGTTTGCCGCAATAGCAACTGACTGATGGACCATCTTCCTGCTCCGTATAGCCTGGCAACGTGATATGACCGCATTCGGCAGCATTTCCCCAGGCCCCTATAAACGGTCGTT  >  NZ_CP022686/2572586‑2572850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A