Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A27 F36 I0 R1 164 7919.5 7665810 98.4% 7543157 135.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 2,361,489 T→G 14.1% V188G (GTT→GGT)  nikA → nickel ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226862,361,4890TG14.1% 212.6 / 14.4 130V188G (GTT→GGT) nikAnickel ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (46/64);  new base G (17/3);  total (63/67)
Fisher's exact test for biased strand distribution p-value = 4.45e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 134 total aligned reads displayed.

CACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGTGATCCCGGACCCGACTACCCGCGCGGTGGCGTTTGAAACTGG  >  NZ_CP022686/2361352‑2361627
                                                                                                                                         |                                                                                                                                          
cACCCTGAACAGCGCCTAATATCCTTTCCTGCAATAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGAACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGttt                                                                                                                                          <  5:373731/140‑1 (MQ=255)
cACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGAAGTTTAAAAACCACCAACCCATGAACGGAATAAAAGCGCCGAATGGCACCGCGCCGTGGGttt                                                                                                                                          >  2:308942/1‑140 (MQ=255)
   ccTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTATAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTAc                                                                                                                                       >  2:804301/1‑140 (MQ=255)
   ccTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGGTTTaa                                                                                                                                       >  2:1877/1‑139 (MQ=255)
    cTGAAAAGCTCATACTCTCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTCTCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGTAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACa                                                                                                                                      <  2:72346/140‑1 (MQ=255)
    cTGAAAAGCGCCTACTATCCTTTGCTGCAATAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCTCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACa                                                                                                                                      <  4:665665/140‑1 (MQ=255)
    cTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAACCCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGGTTTGCa                                                                                                                                      <  8:158986/140‑1 (MQ=255)
     tGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGGTTTagag                                                                                                                                     >  7:881413/1‑140 (MQ=255)
     tGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCACCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGCAATTAAAGCGCCGAATGGCACCGGGCCGTGGGTTTTaaac                                                                                                                                     >  7:397351/1‑137 (MQ=255)
            cgcCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGTAATCGa                                                                                                                              >  7:184132/1‑140 (MQ=255)
            cgcCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGACCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACAAAGAACGCAAATAAAACGCCGATTTGAACCCGTCCGAGGGTTTTACATGAAtaca                                                                                                                              >  2:131040/1‑137 (MQ=255)
             gcCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGGTTTTAAGGAATCgca                                                                                                                             >  8:401680/1‑138 (MQ=255)
             gcCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAAACACGAAACCATGAACGGAAATAAAGCGCAGATTGGCACCGGGCCGTGGGTTTTACAGGAATAGaa                                                                                                                             >  4:454222/1‑140 (MQ=255)
              ccTACTATCCTTTCGTGCAAGAAGTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACTAAACCATGACCGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGaaa                                                                                                                            <  3:441342/140‑1 (MQ=255)
              catactaTCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGaaa                                                                                                                            <  1:105395/138‑1 (MQ=255)
              catactaTCCTTACCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGaaa                                                                                                                            <  5:830509/138‑1 (MQ=255)
               ctactaTCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCCCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCGGATTGGCACCGGTCCGTGGGTTTTACAGGAATCGAAAc                                                                                                                           <  8:711809/140‑1 (MQ=255)
                    aTCCTTTCCTGGAAGAACTGGCCCTGCCCCGTGCTTTCCGTTTTCTCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGCATTAAAGCGCCGATTGGCACCGGGCCGTGGGGTTTACAGGAATCGAAACTGAAt                                                                                                                      <  8:607859/140‑1 (MQ=255)
                    aTCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAAt                                                                                                                      <  5:499617/140‑1 (MQ=255)
                    aTCCTTTCCTGCAAGAACTGGCCCGGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAAt                                                                                                                      <  1:131516/140‑1 (MQ=255)
                         ttCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGATTAAAAACCACGAAACCAAGAACGGAATTAAAGCGCCGATTTGCACCGGTCCGTGGGTTTTACATGAATGGGAACTGAATCAgca                                                                                                                 >  7:416874/1‑138 (MQ=255)
                          tCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCCCCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGGTTTACCCGAATAGCAAATGAAACAGTAc                                                                                                                >  3:132646/1‑140 (MQ=255)
                          tCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGTTTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAAAAGAATCAGAAc                                                                                                                >  1:110541/1‑140 (MQ=255)
                          tCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGCTTGGCCCCGGGCCGTGGGGTTTACAGGGAACGCAACTGAATAAGTAc                                                                                                                >  5:98137/1‑140 (MQ=255)
                          tCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGGTTTTCAGGAATAGAAAAGGAATGAGTAc                                                                                                                >  6:460494/1‑140 (MQ=255)
                          tCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGGTTTAAAGGAATCGAAACTGAATAAGTaa                                                                                                                >  7:740582/1‑139 (MQ=255)
                          tCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCAGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAATCGCCGATTGGCACCGGGCCGAGGGGTTTACAGGGAACGAAACAGACTAAGTAc                                                                                                                >  7:483510/1‑140 (MQ=255)
                          tCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCAGTTTTATCGCAACCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATAAAAGCGCCGATTGGCACCGGGCCGTGGGGTTTACAGGAATCGAAACTGaagaagaaa                                                                                                                >  1:549794/1‑133 (MQ=255)
                           cctgataGAACTGGCGCTGCCCCGTCCTTTCCGGTTTATCGCACTCTCGCATTTTAAAAACGCGGAAACCTTGAATGTATTTAAAGCGCCGATTGGCTCCGGGGCGTTGGTTTTACAGGAATCGAAACTGAATCAGTACg                                                                                                               <  6:47219/134‑1 (MQ=255)
                           ccTGCAAGAACTTGCCCTGCCCAGTCCTTTCCTTTTTATCGCACCCTCGCAGTTTAAAAACCTCGAAACCATGAACTGAATTAAAGCGCCGCTTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACg                                                                                                               <  8:554336/140‑1 (MQ=255)
                           ccTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACg                                                                                                               <  1:731961/140‑1 (MQ=255)
                           ccTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACg                                                                                                               <  3:702756/140‑1 (MQ=255)
                           ccTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACg                                                                                                               <  1:309602/140‑1 (MQ=255)
                           ccTGCAAGAACTGGCCCTGACCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACg                                                                                                               <  5:477532/140‑1 (MQ=255)
                           ccTGCAAGAAATGGCCCTGCCCCGTCCTTACCGTTTTAGCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACg                                                                                                               <  7:13484/140‑1 (MQ=255)
                               cAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGGTTTAGAGGAATCGAAACTGAATCACTAAGATGt                                                                                                           >  3:273989/1‑140 (MQ=255)
                                  gAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCATCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGACGATTGGCGCCGGGCCGTGGGGTTTTCAGGCATAGAATAAGAATCAGTAAGATTTCtt                                                                                                        >  6:374882/1‑140 (MQ=255)
                                    aCTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGGATTCg                                                                                                      >  4:868731/1‑140 (MQ=255)
                                       ggCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACTGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGGTTTTAAGGACTCGAAACTGAATCAGTAAGATGTGTTCGTcc                                                                                                   >  7:621322/1‑140 (MQ=255)
                                        gCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGGTTTTCAGGGATCGAAAAGGAATAAGTACGATGTCTTAGTGCg                                                                                                  >  1:196846/1‑140 (MQ=255)
                                          ccTGCCCCGTCCTTACCGTTTTATCGCCCCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTa                                                                                                <  3:773832/140‑1 (MQ=255)
                                             gCCGCGCCCTTTCCGTTTTTTCGCCCCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGGGGGTGTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACg                                                                                             <  6:23341/140‑1 (MQ=255)
                                             gCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTTAAGGAATCCAAAAATAATAACTACGATGTCTTCGGCCGTAACg                                                                                             >  7:330639/1‑140 (MQ=255)
                                             gCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTAAATGAATCGAAACTTAATCAGTACGATGTCTTAGTCCGTAAcc                                                                                             >  7:438012/1‑139 (MQ=255)
                                             gCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTAAAGGCATCGAAACTGAATGAGTACGATGTCTTCGGGCGTTACg                                                                                             >  6:11913/1‑140 (MQ=255)
                                                cactCCTTTCCGTTTTATCGCACCCTAGAATTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGaaa                                                                                          >  8:383764/4‑140 (MQ=255)
                                                  gTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTTCAGGCATCCTAACAGAATAAGTTACATGTCTTCCTACGaaaacaaaac                                                                                        >  6:893850/1‑140 (MQ=255)
                                                  gTCCTTTCCGTTTTATCGCAACCTCTCAGTTTAAAAACCACGAACCCATGCTCGGAATTAAACCGCCGATTGTCACCGGGCCGTGGGTTTTACATGTATAGAACAAGATAAACTACCATTTCTTCGTCCGTAACGaaaaa                                                                                        >  2:658407/1‑139 (MQ=255)
                                                   tCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTGGTGCAGGCATCGAAACTGAATCATTAAGATGTAGTCGTCCGGAGCGAAAact                                                                                       >  7:259007/1‑140 (MQ=255)
                                                        tCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACATGCAAACAAACTGAAAAAATAAGATGTCTTCGTCCGTAAAGAAAACAACAgg                                                                                  >  2:888369/1‑140 (MQ=255)
                                                        tCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACAACTgg                                                                                  >  8:310842/1‑140 (MQ=255)
                                                        tCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAAAGGAAACTGAAAAAGTACGATGTCTTCGTCCGTAACCAAAACTACTgg                                                                                  >  4:254128/1‑140 (MQ=255)
                                                        tCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCAGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTgg                                                                                  >  1:510764/1‑140 (MQ=255)
                                                        tCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCAGTGGGTTTTAAAGGAAACGAAACAGAAAAAGAACGATTTCATAGTCCGTAAAGAAAACAACAgg                                                                                  >  3:917201/1‑140 (MQ=255)
                                                        tCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGAGCCGATTGGCACCGGGCCGTGGGTTTTAAAGGAATCGGAACTGAATCAGTAAGATGTCTTAGTACGAAACGAAAACTACTgg                                                                                  >  1:603472/1‑140 (MQ=255)
                                                        tCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACAATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACATGAATCGAAACTGAATAAGTAAGATTTCTTCGTACTAAACAAAAACTACTgt                                                                                  >  5:540418/1‑139 (MQ=255)
                                                                    cACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTAAAGGAATAGAAACTTAAACAGTACGATGTATTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCg                                                                      >  4:522541/1‑140 (MQ=255)
                                                                      cccTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGc                                                                    >  7:148292/1‑140 (MQ=255)
                                                                          cGCAGTTTAAAAACCCCGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGGTTTACAGGCATCGAAACTGCATCAGTACGATGTCAGCGTCCGTAACCAAAACAACTGGGGCGCACAGCCGGCGCtt                                                                >  1:678236/1‑140 (MQ=255)
                                                                          cGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTTGCACCGGGCCGTGGGGGTTTCAGGAATCGAAACGGCATAAGTACGATGTCTTGGGCCGGAACGAAAAGAACTGGGGGGAAAAGCAGGCGAtt                                                                >  8:462074/1‑140 (MQ=255)
                                                                           ccAGTTTAAAAACCACGAAACCATTAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTa                                                               <  3:169022/139‑1 (MQ=255)
                                                                                 tAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTTCAGGAATAGCAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTAATGGGGCGAAAAGCCGGCGATTAAAAAGa                                                         >  3:758193/1‑140 (MQ=255)
                                                                                  aataaCCCCGAAACAATGAAATGAATTAAAGCGCAGATTGTCAACGGGCCGTGGGTTTTACATGAATGTAAAATGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGAt                                                        <  8:473736/137‑1 (MQ=255)
                                                                                  aaaaaCTACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGAt                                                        <  3:539557/140‑1 (MQ=255)
                                                                                  aaaaaCCACGAATCCCTTAACGGAATTAAAGCGCCGATTGGCACCGTGCCGTGGGTTTTACAGGATTCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTTGGTCGAAAAGCCGGCGATTAAAAAGAt                                                        <  4:172511/140‑1 (MQ=255)
                                                                                  aaaaaCCACGAACCCATGAACGGAATTACAGCGCCGATTGGCACCGGGCCGTGGGGTTTCCAGGAATCGAAACTGAATCAGAACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCAGGCGATTAAAAAGAt                                                        <  2:734498/140‑1 (MQ=255)
                                                                                  aaaaaCCACGAAACCATTAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGAt                                                        <  3:774785/140‑1 (MQ=255)
                                                                                  aaaaaCCACGAAACCATGATCGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGAt                                                        <  5:185866/140‑1 (MQ=255)
                                                                                  aaaaaCCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGAt                                                        <  1:750978/140‑1 (MQ=255)
                                                                                  aaaaaCCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGAt                                                        <  7:863641/140‑1 (MQ=255)
                                                                                  aaaaaCCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGAt                                                        <  3:602059/140‑1 (MQ=255)
                                                                                  aaaaaCCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGCGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGAt                                                        <  2:173952/140‑1 (MQ=255)
                                                                                   aaaaCCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGTGCCGTGGGGTTTAAACGAATCGAAACACAATCAGTACGAAGTCGTAGTCAGTAACGAAAACAAAACGGGCGAAAAGCCCACGAATAAAAAGaaa                                                       >  6:511685/1‑138 (MQ=255)
                                                                                        cACGCAACCATGAACGGAATTAAATCGCCGATTGGCACCGGGCCGTGTGTTTTACAGGAATCGAAACTGACTCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCtt                                                  <  7:563678/140‑1 (MQ=255)
                                                                                        cACGAAACCATTCACGGAATTAACGCGCCGATTGTCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGGACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCtt                                                  <  5:224832/140‑1 (MQ=255)
                                                                                         aCGAAACCATGCACGGAATTATAGCGCCGATTGGCACCGGGCCGGGGGTTTTACAGGAATCGAAACTGCTTCAGTACGATGTCTTCGTCGGTAACGAAATCTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCttt                                                 <  6:126105/140‑1 (MQ=255)
                                                                                         aCGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCttt                                                 >  5:820724/1‑140 (MQ=255)
                                                                                              aCCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGt                                            <  2:637299/140‑1 (MQ=255)
                                                                                              aCCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGt                                            <  3:191457/140‑1 (MQ=255)
                                                                                                     aCGGAATTAAAGCGCCGATTGGCACCGGGACGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGACGGCGATTAAAAAGATCACCTTTAACGTGATccag                                     <  2:631625/140‑3 (MQ=255)
                                                                                                      cGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACTAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGTGATCCCgg                                    <  6:269926/140‑1 (MQ=255)
                                                                                                      cGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGTGATCCCgg                                    <  5:393917/140‑1 (MQ=255)
                                                                                                        taaTTAAAGCGCAGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGTGATCCCGGAc                                  <  7:614267/139‑1 (MQ=255)
                                                                                                         aaTTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTTCAGGTATCGAAACGGAATCAGTACGCTGTCTTCGGCCGGAACGAAAACAACTGGGGCGAAACGCCCGCGATTAAAAAGAATACCTTTAACGTGCTCACGGccc                                 >  6:552453/1‑140 (MQ=255)
                                                                                                          aTTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGTGATCCCGGAccc                                <  4:433215/140‑1 (MQ=255)
                                                                                                          aTTAAAGAGCCGATTGGCACCGGGCCTTGGGTTTTACAGTAATAGAAAAATAATCAGTAAGATGTATTCGTCCTTAAAGAAAACTACTTTGGCGAAAAGCCGGCGAAAAAAAAGATCACCTTTAACGAGAAACCGGAccc                                >  2:574830/1‑140 (MQ=255)
                                                                                                                ccgcCGATTGGCACCGGGCCGTGGGTTATACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGTCGATTAAAAAGATCACCTTTAACGTGATCCCGGACCCGACTAc                          <  4:969009/139‑1 (MQ=255)
                                                                                                                   ccGATTGGCACCGGGCCGTGGGTTTTACATGAATCGAAACTGAATCATTACGATGTCTTCGTAAGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAATATAACCTTTAACGTGATCCCTGACCCGACTAcccc                       >  8:125025/1‑139 (MQ=255)
                                                                                                                   ccGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGTGATCCCGGACCCGACTAcccc                       >  3:303704/1‑139 (MQ=255)
                                                                                                                   ccGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTAGGCCGTAACGAAAACTACTGGGGCGAAAAGCCCGCGATTAAAAAGATGCGCGTTAACGTGAGCCCGGCCCCGCCTGcccc                       >  6:22156/1‑139 (MQ=255)
                                                                                                                         ggCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGTGATCCCGGACCCGACTACCCGCGCGGt                 <  7:383764/140‑1 (MQ=255)
                                                                                                                          ggaCCGGGCCGTGGGTTTTACAGGAATCGAATCTGAATCAGTACGATGGCTTCGTCCGTAATGAAAACTTCTTGTGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGTGATCCCGGACCCGACTACCCGCGCGGTg                <  1:533820/138‑1 (MQ=255)
                                                                                                                          gCAGCGGGCCGTGGGTTTTACAGGAATCGAAACTGTATCAGTACGATGTCTTCGTCCGTAACTAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTACCGTGATCCCGGACCCGACTACCCGCGCGGTg                <  3:205467/140‑1 (MQ=255)
                                                                                                                          gCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTCCTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGTGATCCCGGACCCGACTACCCGCGCGGTg                <  4:125606/140‑1 (MQ=255)
                                                                                                                            aCCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGTGATCCCGGACCCGACTACCCGCGCGGTGGc              <  8:148292/140‑1 (MQ=255)
                                                                                                                                ggCCGTGGGTTTTTCAGGAATCGCAAATGAATCACTACGATGTCTTAGGACGGATCCAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGTGATACACGACCCGACTACCCGCGCGGTGGCGgtt          >  6:852186/1‑140 (MQ=255)
                                                                                                                                ggCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGTTGTCTTAGTCCGTAACGAAAACTACAGGGGCGAAAAGCCGGCGATTAAAAAGATAACCTTTAACGTGATCCCGGACCCCACTACCCGCGCGGGGGCGttt          >  1:138344/1‑140 (MQ=255)
                                                                                                                                ggCCGTGGGTTTTACAGGAATCGAAAATGAATCAGTACGATGTGTTCGTCCGTAACCAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCCACGTTAACGTGATCCCGGCCCCGACTACCCGCGCGgggggggtt          >  7:619250/1‑135 (MQ=255)
                                                                                                                                     gggggTTTACAGGAATCGAAACTTAATCAGTTCGATGTCTTAGTCAGGACCCAAAACTACTGGGGCGAAAAGCCGGCGATTAAACAGATCACCTTTAACGGGATCACGGCACCGACTACCCGCGCGGTGGCGGTTGAAAc     >  8:831378/2‑140 (MQ=255)
                                                                                                                                        gTTTTACAGTAATCGAAACTGAATCAGTACTATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGTGATCCCGGACCCGACTACCCGCGCGGTGGCGTTTGAAACTgg  <  1:595054/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCACCCTCGCAGTTTAAAAACCACGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGGCCGTGGGTTTTACAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCGGCGATTAAAAAGATCACCTTTAACGTGATCCCGGACCCGACTACCCGCGCGGTGGCGTTTGAAACTGG  >  NZ_CP022686/2361352‑2361627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A