Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 693,739 G→T 89.6% H299N (CAC→AAC)  asnB ← asparagine synthetase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273693,7390GT89.6% 597.8 / 68.2 221H299N (CAC→AAC) asnBasparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base G (12/11);  new base T (104/94);  total (116/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 246 total aligned reads displayed.

CGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATT  >  CP009273/693592‑693888
                                                                                                                                                   |                                                                                                                                                     
cGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAgtgaagttaa                                                                                                                                                     >  2:1027120/1‑150 (MQ=255)
 ggACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAt                                                                                                                                                    >  1:863618/1‑150 (MQ=255)
 ggACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAt                                                                                                                                                    >  1:2284273/1‑150 (MQ=255)
  gACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAtt                                                                                                                                                   <  1:379645/150‑1 (MQ=255)
   aCAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAttt                                                                                                                                                  <  1:2489076/150‑1 (MQ=255)
   aCAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAAttt                                                                                                                                                  <  1:4108684/150‑1 (MQ=255)
    cAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTc                                                                                                                                                 <  2:92256/150‑1 (MQ=255)
    cAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTc                                                                                                                                                 <  2:1114280/150‑1 (MQ=255)
     aGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCg                                                                                                                                                >  1:3958159/1‑150 (MQ=255)
     aGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCg                                                                                                                                                >  2:1217476/1‑150 (MQ=255)
     aGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCg                                                                                                                                                >  1:2887398/1‑150 (MQ=255)
     aGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCGTCGTGTGGAGTGTAGTTAATTTCg                                                                                                                                                >  1:1472110/1‑150 (MQ=255)
       cACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTg                                                                                                                                              >  2:4189666/1‑150 (MQ=255)
       cACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTg                                                                                                                                              <  2:2971023/150‑1 (MQ=255)
        aCCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGa                                                                                                                                             <  2:1416003/150‑1 (MQ=255)
        aCCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGCACAGTGAAGTTAATTTCGTGa                                                                                                                                             <  2:484707/150‑1 (MQ=255)
         ccATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGAt                                                                                                                                            >  2:4318937/1‑150 (MQ=255)
            ttttAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCa                                                                                                                                         <  1:2927951/150‑1 (MQ=255)
             tttAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCAc                                                                                                                                        <  2:571141/150‑1 (MQ=255)
                  tGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGc                                                                                                                                   <  2:3392269/150‑1 (MQ=255)
                    cccATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGccc                                                                                                                                 <  1:2172992/150‑1 (MQ=255)
                    cccATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGccc                                                                                                                                 <  2:2297688/150‑1 (MQ=255)
                     ccATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCa                                                                                                                                <  1:355063/150‑1 (MQ=255)
                       aTCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGa                                                                                                                              >  1:845639/1‑150 (MQ=255)
                           ccTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGt                                                                                                                          >  1:3637866/1‑150 (MQ=255)
                            cTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGtt                                                                                                                         <  1:2802115/150‑1 (MQ=255)
                            cTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGtt                                                                                                                         <  1:2801929/150‑1 (MQ=255)
                            cTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGtt                                                                                                                         <  2:4356634/150‑1 (MQ=255)
                             ttGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGttt                                                                                                                        >  1:4319382/1‑150 (MQ=255)
                                   ttACGCGACATTAAATACATCGGTGTTGATGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACt                                                                                                                  <  2:1842820/150‑1 (MQ=255)
                                    tACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACtt                                                                                                                 >  2:2309238/1‑150 (MQ=255)
                                         gACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTgg                                                                                                            >  1:331776/1‑150 (MQ=255)
                                         gACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTgg                                                                                                            <  1:3018673/150‑1 (MQ=255)
                                            aTTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgct                                                                                                         >  1:3487318/1‑150 (MQ=255)
                                              tAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgctgc                                                                                                       <  1:1912709/150‑1 (MQ=255)
                                               aaaTACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgctgct                                                                                                      >  2:4444786/1‑150 (MQ=255)
                                                 aTACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCttt                                                                                                    >  2:1296253/1‑150 (MQ=255)
                                                   aCATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCa                                                                                                  >  1:1365547/1‑150 (MQ=255)
                                                   aCATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTACTTTCa                                                                                                  <  1:2992297/150‑1 (MQ=255)
                                                    cATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAg                                                                                                 >  1:4291612/1‑150 (MQ=255)
                                                     aTCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGa                                                                                                >  2:3835900/1‑150 (MQ=255)
                                                     aTCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGa                                                                                                >  2:1342652/1‑150 (MQ=255)
                                                         gtgtTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCg                                                                                            <  2:2268886/150‑1 (MQ=255)
                                                         gtgtTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCg                                                                                            <  1:804956/150‑1 (MQ=255)
                                                           gtTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGt                                                                                          <  1:4146854/150‑1 (MQ=255)
                                                              gAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGaa                                                                                       <  2:328513/150‑1 (MQ=255)
                                                              gAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGaa                                                                                       >  1:776978/1‑150 (MQ=255)
                                                               aaGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAAc                                                                                      >  2:3231460/1‑150 (MQ=255)
                                                                aGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAAcc                                                                                     >  2:1465864/1‑150 (MQ=255)
                                                                aGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAAcc                                                                                     <  1:4507365/150‑1 (MQ=255)
                                                                   gcgAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCgg                                                                                  <  1:199732/150‑1 (MQ=255)
                                                                    cgAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGc                                                                                 >  1:2603042/1‑150 (MQ=255)
                                                                         aGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGAcc                                                                            >  1:741041/1‑150 (MQ=255)
                                                                            ggTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTAc                                                                         >  2:3047291/1‑150 (MQ=255)
                                                                              tCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAg                                                                       <  1:4609884/150‑1 (MQ=255)
                                                                                acaTCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCa                                                                     >  2:4583521/1‑150 (MQ=255)
                                                                                acaTCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCa                                                                     <  2:3608370/150‑1 (MQ=255)
                                                                                acaTCATAAGTGTCGATGTGGTACATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCa                                                                     <  2:3608968/150‑1 (MQ=255)
                                                                                 catcatAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCaa                                                                    <  2:2356478/150‑1 (MQ=255)
                                                                                      tAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAg                                                               >  2:204929/1‑150 (MQ=255)
                                                                                            tCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAAc                                                         >  2:2375254/1‑150 (MQ=255)
                                                                                             cGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACt                                                        <  1:309796/150‑1 (MQ=255)
                                                                                             cGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACt                                                        <  1:268453/150‑1 (MQ=255)
                                                                                              gATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTg                                                       >  1:4219794/1‑150 (MQ=255)
                                                                                               aTGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGc                                                      <  2:4246576/150‑1 (MQ=255)
                                                                                               aTGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGc                                                      >  1:3145537/1‑150 (MQ=255)
                                                                                                tgtgGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCg                                                     <  2:1764500/150‑1 (MQ=255)
                                                                                                 gtgGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCgg                                                    <  2:774510/150‑1 (MQ=255)
                                                                                                 gtgGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCgg                                                    >  2:2179313/1‑150 (MQ=255)
                                                                                                       aaTCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGccacca                                              <  1:1215729/150‑1 (MQ=255)
                                                                                                               cgcgGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCag                                      >  2:3123240/1‑150 (MQ=255)
                                                                                                                gcgGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCaga                                     <  2:4507595/150‑1 (MQ=255)
                                                                                                                 cgGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCagag                                    <  2:3895946/150‑1 (MQ=255)
                                                                                                                 cgGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCagag                                    >  1:548111/1‑150 (MQ=255)
                                                                                                                     tGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGtt                                <  1:656557/150‑1 (MQ=255)
                                                                                                                      ggCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTc                               >  2:1536873/1‑150 (MQ=255)
                                                                                                                      ggCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGAGTAACTGCGGCCACCAGGCTTCAGAGCGTTc                               >  1:2116879/1‑150 (MQ=255)
                                                                                                                      ggCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTACCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTc                               >  1:4463901/1‑150 (MQ=255)
                                                                                                                         aTCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTg                            <  2:3724322/150‑1 (MQ=255)
                                                                                                                         aTCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTg                            <  2:1593450/150‑1 (MQ=255)
                                                                                                                          tCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGa                           <  1:1027120/150‑1 (MQ=255)
                                                                                                                             aGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCt                        <  1:3669605/150‑1 (MQ=255)
                                                                                                                                 cTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCa                    <  2:825082/150‑1 (MQ=255)
                                                                                                                                   tCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCAcg                  <  2:3795339/150‑1 (MQ=255)
                                                                                                                                    ccTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCAcgc                 <  2:3471223/150‑1 (MQ=255)
                                                                                                                                        tACAGTGAAGTTAATTTCGTGATACACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACg             <  1:3200454/150‑1 (MQ=255)
                                                                                                                                         aCAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACgg            >  1:1588365/1‑150 (MQ=255)
                                                                                                                                          cAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACggg           <  2:741041/150‑1 (MQ=255)
                                                                                                                                          cAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACggg           <  1:3046975/150‑1 (MQ=255)
                                                                                                                                           agtgaagttaATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGc          <  1:3367799/150‑1 (MQ=255)
                                                                                                                                            gtgaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCt         <  2:2873308/150‑1 (MQ=255)
                                                                                                                                            gtgaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCt         >  1:2550105/1‑150 (MQ=255)
                                                                                                                                            gtgaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCt         <  1:3164159/150‑1 (MQ=255)
                                                                                                                                             tgaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTg        <  2:3463534/150‑1 (MQ=255)
                                                                                                                                              gtagtgaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCAGGTGACCCCGGCAGTCATACAGCAAATGAGTGTAACTGCGGCCACCAGGCTTCAGTGAGTTCCTGTTCTTGCACGCGACGGGCTGc       <  2:2708231/148‑1 (MQ=255)
                                                                                                                                              gaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGc       <  1:1577282/150‑1 (MQ=255)
                                                                                                                                              gaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGc       <  2:1762209/150‑1 (MQ=255)
                                                                                                                                              gaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGc       <  2:1827307/150‑1 (MQ=255)
                                                                                                                                               aagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAATGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCg      <  2:2966194/150‑1 (MQ=255)
                                                                                                                                                   gaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTAtt  <  1:1734061/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATT  >  CP009273/693592‑693888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATT  >  CP009273/693590‑693888
                                                                                                                                                     |                                                                                                                                                     
ACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTT                                                                                                                                                       >  SRR19973072.1721566/1‑150 (MQ=60)
   GGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAT                                                                                                                                                    >  SRR19973072.865476/1‑150 (MQ=60)
   GGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAT                                                                                                                                                    >  SRR19973072.2289170/1‑150 (MQ=60)
   GGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTATCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAT                                                                                                                                                    >  SRR19973072.865864/1‑150 (MQ=60)
    GACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATT                                                                                                                                                   <  SRR19973072.2782645/150‑1 (MQ=60)
    GACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATT                                                                                                                                                   <  SRR19973072.380468/150‑1 (MQ=60)
     ACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTT                                                                                                                                                  <  SRR19973072.2494483/150‑1 (MQ=60)
     ACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTT                                                                                                                                                  <  SRR19973072.4117311/150‑1 (MQ=60)
      CAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTC                                                                                                                                                 <  SRR19973072.2250707/150‑1 (MQ=60)
       AGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCG                                                                                                                                                >  SRR19973072.1148844/1‑150 (MQ=60)
       AGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCG                                                                                                                                                >  SRR19973072.2893588/1‑150 (MQ=60)
       AGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCG                                                                                                                                                >  SRR19973072.762434/1‑150 (MQ=60)
       AGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCG                                                                                                                                                >  SRR19973072.3966661/1‑150 (MQ=60)
       AGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCG                                                                                                                                                >  SRR19973072.3966427/1‑150 (MQ=60)
           CCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAATTACATCGGTGTTGAAGCGCGAATTGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGAT                                                                                                                                            <  SRR19973072.619693/150‑1 (MQ=60)
            CATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATG                                                                                                                                           <  SRR19973072.4249985/150‑1 (MQ=60)
            CATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATG                                                                                                                                           >  SRR19973072.217620/1‑150 (MQ=60)
              TTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCA                                                                                                                                         <  SRR19973072.2934216/150‑1 (MQ=60)
               TTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCAC                                                                                                                                        >  SRR19973072.3249523/1‑150 (MQ=60)
                  AATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGT                                                                                                                                     >  SRR19973072.2879466/1‑150 (MQ=60)
                  AATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGT                                                                                                                                     >  SRR19973072.3683217/1‑150 (MQ=60)
                  AATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGT                                                                                                                                     <  SRR19973072.298871/150‑1 (MQ=60)
                      CCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCC                                                                                                                                 <  SRR19973072.2177727/150‑1 (MQ=60)
                       CCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCA                                                                                                                                <  SRR19973072.355850/150‑1 (MQ=60)
                       CCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCA                                                                                                                                >  SRR19973072.234589/1‑150 (MQ=60)
                        CATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAG                                                                                                                               >  SRR19973072.326362/1‑150 (MQ=60)
                        CATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAG                                                                                                                               >  SRR19973072.816161/1‑150 (MQ=60)
                         ATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGA                                                                                                                              >  SRR19973072.847460/1‑150 (MQ=60)
                           CGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATG                                                                                                                            >  SRR19973072.3117532/1‑150 (MQ=60)
                             CCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGT                                                                                                                          >  SRR19973072.3645534/1‑150 (MQ=60)
                              CTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTT                                                                                                                         >  SRR19973072.430851/1‑150 (MQ=60)
                              CTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTT                                                                                                                         <  SRR19973072.2808126/150‑1 (MQ=60)
                              CTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTT                                                                                                                         <  SRR19973072.2808099/150‑1 (MQ=60)
                              CTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTT                                                                                                                         <  SRR19973072.2807930/150‑1 (MQ=60)
                              CTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTT                                                                                                                         <  SRR19973072.2808116/150‑1 (MQ=60)
                               TTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTT                                                                                                                        <  SRR19973072.4224675/150‑1 (MQ=60)
                               TTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTT                                                                                                                        >  SRR19973072.4328416/1‑150 (MQ=60)
                                TGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTG                                                                                                                       >  SRR19973072.2037892/1‑150 (MQ=60)
                                     TTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTTGTCACATCATAAGTTTCGATGTGGTAAATCACGGCGCGGATGGCAACCAGACCTTCCTGTACAGTGAAGTTAATTACGTGATGCACCGTGCCCAGATGGTATGCCACT                                                                                                                  >  SRR19973072.1895717/1‑150 (MQ=60)
                                        CGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCC                                                                                                               >  SRR19973072.4427002/1‑150 (MQ=60)
                                           GACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGG                                                                                                            <  SRR19973072.3025082/150‑1 (MQ=60)
                                           GACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGG                                                                                                            >  SRR19973072.332523/1‑150 (MQ=60)
                                              ATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCT                                                                                                         >  SRR19973072.3494609/1‑150 (MQ=60)
                                                TAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGC                                                                                                       <  SRR19973072.1916783/150‑1 (MQ=60)
                                                TAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGAGGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGC                                                                                                       <  SRR19973072.4405022/150‑1 (MQ=60)
                                                     ACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCA                                                                                                  >  SRR19973072.1368531/1‑150 (MQ=60)
                                                     ACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTACTTTCA                                                                                                  <  SRR19973072.2998617/150‑1 (MQ=60)
                                                     ACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTACTTTCA                                                                                                  <  SRR19973072.2998671/150‑1 (MQ=60)
                                                      CATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAG                                                                                                 >  SRR19973072.4300552/1‑150 (MQ=60)
                                                           GTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCG                                                                                            <  SRR19973072.806681/150‑1 (MQ=60)
                                                             GTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGT                                                                                          <  SRR19973072.4155536/150‑1 (MQ=60)
                                                             GTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGT                                                                                          <  SRR19973072.4155548/150‑1 (MQ=60)
                                                               TGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGA                                                                                        <  SRR19973072.2279326/150‑1 (MQ=60)
                                                               TGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGA                                                                                        >  SRR19973072.743116/1‑150 (MQ=60)
                                                               TGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGA                                                                                        >  SRR19973072.4008551/1‑150 (MQ=60)
                                                                GAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAA                                                                                       >  SRR19973072.778626/1‑150 (MQ=60)
                                                                  AGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACC                                                                                     <  SRR19973072.4516843/150‑1 (MQ=60)
                                                                  AGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACC                                                                                     <  SRR19973072.4516882/150‑1 (MQ=60)
                                                                    CGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCG                                                                                   <  SRR19973072.4540950/150‑1 (MQ=60)
                                                                     GCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGG                                                                                  <  SRR19973072.200235/150‑1 (MQ=60)
                                                                     GCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGG                                                                                  <  SRR19973072.2521668/150‑1 (MQ=60)
                                                                      CGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGC                                                                                 >  SRR19973072.3035371/1‑150 (MQ=60)
                                                                      CGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGC                                                                                 >  SRR19973072.2608653/1‑150 (MQ=60)
                                                                        AATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAG                                                                               >  SRR19973072.3636129/1‑150 (MQ=60)
                                                                        AATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAG                                                                               <  SRR19973072.2727811/150‑1 (MQ=60)
                                                                        AATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGAACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAG                                                                               >  SRR19973072.3636138/1‑150 (MQ=60)
                                                                           AGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACC                                                                            >  SRR19973072.742628/1‑150 (MQ=60)
                                                                                TCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAG                                                                       >  SRR19973072.3862645/1‑150 (MQ=60)
                                                                                TCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAG                                                                       <  SRR19973072.4619533/150‑1 (MQ=60)
                                                                                TCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAG                                                                       <  SRR19973072.4619559/150‑1 (MQ=60)
                                                                                    ATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAA                                                                   <  SRR19973072.3408739/150‑1 (MQ=60)
                                                                                    ATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAA                                                                   >  SRR19973072.178090/1‑150 (MQ=60)
                                                                                    ATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAA                                                                   >  SRR19973072.832211/1‑150 (MQ=60)
                                                                                         AAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTTAACCCGGCAGACCTACAGCAAAGGAGT                                                              >  SRR19973072.3403414/1‑150 (MQ=60)
                                                                                         AAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGT                                                              <  SRR19973072.3746722/150‑1 (MQ=60)
                                                                                         AAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGT                                                              <  SRR19973072.1552956/150‑1 (MQ=60)
                                                                                          AGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTG                                                             >  SRR19973072.3660552/1‑150 (MQ=60)
                                                                                           GTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGT                                                            >  SRR19973072.1315107/1‑150 (MQ=60)
                                                                                             TTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAA                                                          >  SRR19973072.4185479/1‑150 (MQ=60)
                                                                                               CGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACT                                                        <  SRR19973072.310509/150‑1 (MQ=60)
                                                                                               CGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACT                                                        <  SRR19973072.269087/150‑1 (MQ=60)
                                                                                                GATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTG                                                       >  SRR19973072.4228604/1‑150 (MQ=60)
                                                                                                GATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTG                                                       >  SRR19973072.3183528/1‑150 (MQ=60)
                                                                                                GATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTG                                                       >  SRR19973072.3497823/1‑150 (MQ=60)
                                                                                                GATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTG                                                       >  SRR19973072.2568631/1‑150 (MQ=60)
                                                                                                 ATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGC                                                      >  SRR19973072.2630550/1‑150 (MQ=60)
                                                                                                 ATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGC                                                      >  SRR19973072.3152171/1‑150 (MQ=60)
                                                                                                 ATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCAACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGC                                                      >  SRR19973072.3152214/1‑150 (MQ=60)
                                                                                                   GTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGG                                                    <  SRR19973072.938891/150‑1 (MQ=60)
                                                                                                       TAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCAC                                                >  SRR19973072.2999823/1‑150 (MQ=60)
                                                                                                         AATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCA                                              <  SRR19973072.1218327/150‑1 (MQ=60)
                                                                                                             ACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAAATGCGGCCACCAGGCT                                          >  SRR19973072.683120/1‑150 (MQ=60)
                                                                                                              CGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTT                                         >  SRR19973072.2902710/1‑150 (MQ=60)
                                                                                                                   CGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAG                                    >  SRR19973072.551258/1‑150 (MQ=60)
                                                                                                                   CGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAG                                    >  SRR19973072.3025145/1‑150 (MQ=60)
                                                                                                                   CGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAG                                    >  SRR19973072.549404/1‑150 (MQ=60)
                                                                                                                       TGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTT                                <  SRR19973072.658041/150‑1 (MQ=60)
                                                                                                                        GGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTC                               >  SRR19973072.1940983/1‑150 (MQ=60)
                                                                                                                        GGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGAGTAACTGCGGCCACCAGGCTTCAGAGCGTTC                               >  SRR19973072.2121491/1‑150 (MQ=60)
                                                                                                                        GGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTACCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTC                               >  SRR19973072.4473321/1‑150 (MQ=60)
                                                                                                                            TCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGA                           <  SRR19973072.1029348/150‑1 (MQ=60)
                                                                                                                             CCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGAT                          <  SRR19973072.2105100/150‑1 (MQ=60)
                                                                                                                               AGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCT                        <  SRR19973072.3677339/150‑1 (MQ=60)
                                                                                                                                  CCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCC                     >  SRR19973072.4636730/1‑150 (MQ=60)
                                                                                                                                         GTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGAC              >  SRR19973072.1427431/1‑150 (MQ=60)
                                                                                                                                         GTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGAC              >  SRR19973072.1427530/1‑150 (MQ=60)
                                                                                                                                          TACAGTGAAGTTAATTTCGTGATACACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACG             <  SRR19973072.3207216/150‑1 (MQ=60)
                                                                                                                                           ACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGG            >  SRR19973072.1591776/1‑150 (MQ=60)
                                                                                                                                            CAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGG           <  SRR19973072.3053420/150‑1 (MQ=60)
                                                                                                                                            CAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGG           <  SRR19973072.3053509/150‑1 (MQ=60)
                                                                                                                                             AGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGC          <  SRR19973072.3374933/150‑1 (MQ=60)
                                                                                                                                             AGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGC          <  SRR19973072.3374452/150‑1 (MQ=60)
                                                                                                                                              GTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCT         >  SRR19973072.2555640/1‑150 (MQ=60)
                                                                                                                                              GTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCT         <  SRR19973072.3170840/150‑1 (MQ=60)
                                                                                                                                              GTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTATGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCT         >  SRR19973072.2555760/1‑150 (MQ=60)
                                                                                                                                               TGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTG        <  SRR19973072.1677485/150‑1 (MQ=60)
                                                                                                                                                GAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGC       <  SRR19973072.1580854/150‑1 (MQ=60)
                                                                                                                                                GAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGC       <  SRR19973072.1580710/150‑1 (MQ=60)
                                                                                                                                                GAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGC       <  SRR19973072.1580656/150‑1 (MQ=60)
                                                                                                                                                 AAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCG      >  SRR19973072.851173/1‑150 (MQ=60)
                                                                                                                                                 AAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCG      >  SRR19973072.3105289/1‑150 (MQ=60)
                                                                                                                                                    TTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTAT   <  SRR19973072.4641737/150‑1 (MQ=60)
                                                                                                                                                     GAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATT  <  SRR19973072.804692/150‑1 (MQ=60)
                                                                                                                                                     GAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATT  <  SRR19973072.1737858/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATT  >  CP009273/693590‑693888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑