Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 693,739 G→T 100% H299N (CAC→AAC)  asnB ← asparagine synthetase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273693,7390GT97.5% 663.9 / 6.0 214H299N (CAC→AAC) asnBasparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base T (106/101);  total (109/105)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 225 total aligned reads displayed.

GACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTG  >  CP009273/693594‑693882
                                                                                                                                                 |                                                                                                                                               
gACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAgtgaagttaa                                                                                                                                               >  2:2844851/1‑148 (MQ=255)
    gCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTc                                                                                                                                           >  2:3021667/1‑148 (MQ=255)
    gCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGGTGAAGCGCGAATAGTGGTCACATCATACGTTTCGATGTGGTAAATCACGTCGCGGATGTCATCCAGACCTTCCTGTACAGTGACGTTAATTTc                                                                                                                                           >  2:3236263/1‑148 (MQ=255)
     cACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCg                                                                                                                                          >  2:558298/1‑148 (MQ=255)
     cACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCg                                                                                                                                          >  1:2670834/1‑148 (MQ=255)
     cACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCg                                                                                                                                          <  1:1831274/148‑1 (MQ=255)
       ccATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTg                                                                                                                                        <  1:3296762/148‑1 (MQ=255)
           tttAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGc                                                                                                                                    >  1:943642/1‑148 (MQ=255)
              aaTGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCAcc                                                                                                                                 <  1:578999/148‑1 (MQ=255)
              aaTGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCAcc                                                                                                                                 >  2:730362/1‑148 (MQ=255)
              aaTGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCAcc                                                                                                                                 <  2:405470/148‑1 (MQ=255)
                 gCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTg                                                                                                                              >  1:3184981/1‑148 (MQ=255)
                 gCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTg                                                                                                                              <  2:3308822/148‑1 (MQ=255)
                  cccATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGc                                                                                                                             <  2:2962171/148‑1 (MQ=255)
                    cATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGccc                                                                                                                           >  1:2418035/1‑148 (MQ=255)
                    cATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGccc                                                                                                                           >  2:1132102/1‑148 (MQ=255)
                    cATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGccc                                                                                                                           >  1:1823852/1‑148 (MQ=255)
                      tCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAg                                                                                                                         >  2:2946094/1‑148 (MQ=255)
                      tCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAg                                                                                                                         <  2:3103180/148‑1 (MQ=255)
                      tCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAg                                                                                                                         <  1:2893590/148‑1 (MQ=255)
                       cGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGa                                                                                                                        <  2:136095/148‑1 (MQ=255)
                       cGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGa                                                                                                                        <  2:136074/148‑1 (MQ=255)
                         ccTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATg                                                                                                                      >  2:168942/1‑148 (MQ=255)
                           ttGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAttt                                                                                                                                            <  2:1430094/124‑1 (MQ=255)
                           ttGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAttt                                                                                                                                            >  1:1430094/1‑124 (MQ=255)
                           ttGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGt                                                                                                                    >  2:396502/1‑148 (MQ=255)
                           ttGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGt                                                                                                                    <  1:819380/148‑1 (MQ=255)
                            tGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGtt                                                                                                                   >  1:3086150/1‑148 (MQ=255)
                              aTCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTg                                                                                                                 >  2:3431402/1‑148 (MQ=255)
                                     gcgACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTc                                                                                                          <  1:2611866/148‑1 (MQ=255)
                                     gcgACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTc                                                                                                          >  1:320821/1‑148 (MQ=255)
                                      cgACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTcc                                                                                                         <  1:1234062/148‑1 (MQ=255)
                                      cgACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTcc                                                                                                         <  1:2644991/148‑1 (MQ=255)
                                      cgACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGACACTTcc                                                                                                         >  2:1565416/1‑148 (MQ=255)
                                       gACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCt                                                                                                        <  1:3121002/148‑1 (MQ=255)
                                          aTTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTggg                                                                                                     <  1:759831/148‑1 (MQ=255)
                                           ttAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgc                                                                                                    <  2:1066301/148‑1 (MQ=255)
                                            tAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgct                                                                                                   >  1:545060/1‑148 (MQ=255)
                                              aaTACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgctgc                                                                                                 <  1:3021667/148‑1 (MQ=255)
                                               aTACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGgctgct                                                                                                >  2:86156/1‑148 (MQ=255)
                                                     cGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGa                                                                                          <  1:136105/148‑1 (MQ=255)
                                                       gtgtTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATc                                                                                        <  1:396502/148‑1 (MQ=255)
                                                            gAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTg                                                                                   <  1:2996476/148‑1 (MQ=255)
                                                             aaGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGa                                                                                  >  2:292893/1‑148 (MQ=255)
                                                              aGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGaa                                                                                 >  2:3366403/1‑148 (MQ=255)
                                                                       aGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGa                                                                        <  1:3060436/148‑1 (MQ=255)
                                                                         tGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGAcc                                                                      >  1:590644/1‑148 (MQ=255)
                                                                            tCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTAc                                                                   >  2:2248806/1‑148 (MQ=255)
                                                                            tCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTAc                                                                   >  1:234152/1‑148 (MQ=255)
                                                                              acaTCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAg                                                                 >  2:1632058/1‑148 (MQ=255)
                                                                              acaTCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAg                                                                 <  1:3431402/148‑1 (MQ=255)
                                                                               catcatAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGc                                                                >  2:1941004/1‑148 (MQ=255)
                                                                                atcatAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCa                                                               <  2:2418030/148‑1 (MQ=255)
                                                                                atcatAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCa                                                               <  2:1823852/148‑1 (MQ=255)
                                                                                 tcatAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCaa                                                              >  2:8314/1‑148 (MQ=255)
                                                                                 tcatAAGGTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGCTGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCac                                                              >  2:8043/1‑147 (MQ=255)
                                                                                  catAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCaaa                                                             >  2:475524/1‑148 (MQ=255)
                                                                                  catAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCaaa                                                             <  2:68223/148‑1 (MQ=255)
                                                                                   atAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAg                                                            <  1:2505698/148‑1 (MQ=255)
                                                                                     aaGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGa                                                          <  2:1072385/148‑1 (MQ=255)
                                                                                     aaGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGa                                                          >  2:639595/1‑148 (MQ=255)
                                                                                       gTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAgt                                                        >  1:1037979/1‑148 (MQ=255)
                                                                                          tCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTa                                                     <  1:1790134/148‑1 (MQ=255)
                                                                                              tgtgGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTg                                                 <  2:2427963/148‑1 (MQ=255)
                                                                                                tgGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCg                                               >  1:2318987/1‑148 (MQ=255)
                                                                                                      aTCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGccacc                                         >  2:3495859/1‑148 (MQ=255)
                                                                                                      aTCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGccacc                                         <  2:1221627/148‑1 (MQ=255)
                                                                                                       tCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGccacca                                        <  1:424294/148‑1 (MQ=255)
                                                                                                       tCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGccacca                                        >  2:521359/1‑148 (MQ=255)
                                                                                                       tCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGccacca                                        >  1:3136710/1‑148 (MQ=255)
                                                                                                       tCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGccacca                                        >  1:1883381/1‑148 (MQ=255)
                                                                                                        cACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAg                                       <  1:2121611/148‑1 (MQ=255)
                                                                                                            tCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCtt                                   <  2:702909/148‑1 (MQ=255)
                                                                                                               cgGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCag                                <  1:2456330/148‑1 (MQ=255)
                                                                                                                ggATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCaga                               <  1:456760/148‑1 (MQ=255)
                                                                                                                ggATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCaga                               <  1:1481487/148‑1 (MQ=255)
                                                                                                                   tGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCg                            <  1:732708/148‑1 (MQ=255)
                                                                                                                   tGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGATTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCg                            >  1:1923324/1‑148 (MQ=255)
                                                                                                                    ggCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGt                           >  2:280300/1‑148 (MQ=255)
                                                                                                                    ggCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGt                           <  2:3135815/148‑1 (MQ=255)
                                                                                                                        tCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCt                       >  1:1258095/1‑148 (MQ=255)
                                                                                                                         ccAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTg                      >  2:2605459/1‑148 (MQ=255)
                                                                                                                          cAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGa                     <  1:8533/148‑1 (MQ=255)
                                                                                                                          cAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGa                     <  2:234152/148‑1 (MQ=255)
                                                                                                                             aCCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCt                  >  2:1857602/1‑148 (MQ=255)
                                                                                                                              ccTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCtt                 >  1:1538436/1‑148 (MQ=255)
                                                                                                                              ccTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCtt                 <  2:2877039/148‑1 (MQ=255)
                                                                                                                               cTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTc                >  1:2692647/1‑148 (MQ=255)
                                                                                                                                ttCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTcc               <  1:2838602/148‑1 (MQ=255)
                                                                                                                                 tCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCa              >  2:4248/1‑148 (MQ=255)
                                                                                                                                   cTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCAcg            >  1:3250281/1‑148 (MQ=255)
                                                                                                                                     gTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCAcgcg          >  1:3467138/1‑148 (MQ=255)
                                                                                                                                      tACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGa         <  1:1565416/148‑1 (MQ=255)
                                                                                                                                      tACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGa         <  1:712086/148‑1 (MQ=255)
                                                                                                                                          gtgaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACggg     <  1:2970304/148‑1 (MQ=255)
                                                                                                                                          gtgaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACggg     >  2:2052520/1‑148 (MQ=255)
                                                                                                                                          gtgaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACggg     >  2:2050537/1‑148 (MQ=255)
                                                                                                                                           tgaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGc    <  2:776458/148‑1 (MQ=255)
                                                                                                                                           tgaagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGc    >  1:2486920/1‑148 (MQ=255)
                                                                                                                                             aagttaaTTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTg  <  1:292893/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTG  >  CP009273/693594‑693882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATT  >  CP009273/693590‑693888
                                                                                                                                                     |                                                                                                                                                     
ACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAATCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTT                                                                                                                                                       <  SRR19973065.3361408/150‑1 (MQ=60)
ACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTT                                                                                                                                                       >  SRR19973065.2894658/1‑150 (MQ=60)
 CCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTA                                                                                                                                                      <  SRR19973065.3305098/150‑1 (MQ=60)
   GGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAAT                                                                                                                                                    <  SRR19973065.2703956/150‑1 (MQ=60)
     ACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTT                                                                                                                                                  <  SRR19973065.643706/150‑1 (MQ=60)
     ACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTT                                                                                                                                                  >  SRR19973065.2381163/1‑150 (MQ=60)
     ACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTT                                                                                                                                                  <  SRR19973065.3243913/150‑1 (MQ=60)
      CAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTC                                                                                                                                                 >  SRR19973065.1922856/1‑150 (MQ=60)
       AGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCG                                                                                                                                                >  SRR19973065.2687373/1‑150 (MQ=60)
       AGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCG                                                                                                                                                >  SRR19973065.2687380/1‑150 (MQ=60)
        GCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGT                                                                                                                                               <  SRR19973065.3238196/150‑1 (MQ=60)
         CACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTG                                                                                                                                              <  SRR19973065.1843339/150‑1 (MQ=60)
          ACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGA                                                                                                                                             >  SRR19973065.781620/1‑150 (MQ=60)
           CCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGAT                                                                                                                                            <  SRR19973065.3316841/150‑1 (MQ=60)
            CATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATG                                                                                                                                           <  SRR19973065.434489/150‑1 (MQ=60)
             ATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGC                                                                                                                                          >  SRR19973065.949878/1‑150 (MQ=60)
                  AATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGT                                                                                                                                     <  SRR19973065.582625/150‑1 (MQ=60)
                  AATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGT                                                                                                                                     <  SRR19973065.582622/150‑1 (MQ=60)
                  AATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGT                                                                                                                                     <  SRR19973065.582615/150‑1 (MQ=60)
                   ATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTG                                                                                                                                    >  SRR19973065.3204212/1‑150 (MQ=60)
                    TGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGC                                                                                                                                   >  SRR19973065.1312564/1‑150 (MQ=60)
                    TGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGC                                                                                                                                   >  SRR19973065.2852673/1‑150 (MQ=60)
                    TGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGC                                                                                                                                   >  SRR19973065.964665/1‑150 (MQ=60)
                      CCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCC                                                                                                                                 >  SRR19973065.2433264/1‑150 (MQ=60)
                      CCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCC                                                                                                                                 >  SRR19973065.2433269/1‑150 (MQ=60)
                      CCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCC                                                                                                                                 >  SRR19973065.3134930/1‑150 (MQ=60)
                      CCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCC                                                                                                                                 >  SRR19973065.1835863/1‑150 (MQ=60)
                          TCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGAT                                                                                                                             <  SRR19973065.2911264/150‑1 (MQ=60)
                             CCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGagatcggaagagcacacgtctg                                                                                                                          >  SRR19973065.1439395/1‑128 (MQ=60)
                              CTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTT                                                                                                                         >  SRR19973065.2729383/1‑150 (MQ=60)
                              CTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTT                                                                                                                         >  SRR19973065.965658/1‑150 (MQ=60)
                              CTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTT                                                                                                                         >  SRR19973065.3104997/1‑150 (MQ=60)
                              CTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTT                                                                                                                         <  SRR19973065.2986930/150‑1 (MQ=60)
                               TTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTT                                                                                                                        <  SRR19973065.824766/150‑1 (MQ=60)
                               TTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGCGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTT                                                                                                                        <  SRR19973065.2473668/150‑1 (MQ=60)
                                 GATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGC                                                                                                                      >  SRR19973065.398660/1‑150 (MQ=60)
                                 GATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGC                                                                                                                      >  SRR19973065.398594/1‑150 (MQ=60)
                                 GATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGC                                                                                                                      >  SRR19973065.18881/1‑150 (MQ=60)
                                       ACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTC                                                                                                                >  SRR19973065.323037/1‑150 (MQ=60)
                                        CGCGAGATTAAATACATCGGTGTTGAAGCGCGAATAGTTGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACATTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCC                                                                                                               <  SRR19973065.3012724/150‑1 (MQ=60)
                                         GCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCT                                                                                                              <  SRR19973065.2628060/150‑1 (MQ=60)
                                         GCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCT                                                                                                              <  SRR19973065.2628072/150‑1 (MQ=60)
                                          CGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTG                                                                                                             <  SRR19973065.2661302/150‑1 (MQ=60)
                                          CGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTG                                                                                                             <  SRR19973065.1242410/150‑1 (MQ=60)
                                          CGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTG                                                                                                             <  SRR19973065.1242147/150‑1 (MQ=60)
                                           GACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGG                                                                                                            <  SRR19973065.3139971/150‑1 (MQ=60)
                                           GACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGG                                                                                                            <  SRR19973065.3140012/150‑1 (MQ=60)
                                           GACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGG                                                                                                            <  SRR19973065.3141133/150‑1 (MQ=60)
                                              ATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCT                                                                                                         >  SRR19973065.548584/1‑150 (MQ=60)
                                              ATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCT                                                                                                         <  SRR19973065.764895/150‑1 (MQ=60)
                                              ATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCT                                                                                                         <  SRR19973065.764463/150‑1 (MQ=60)
                                                  AATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTT                                                                                                     <  SRR19973065.3040181/150‑1 (MQ=60)
                                                     ACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCNGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCA                                                                                                  <  SRR19973065.1795338/150‑1 (MQ=60)
                                                         CGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATC                                                                                              <  SRR19973065.137270/150‑1 (MQ=60)
                                                          GGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCC                                                                                             <  SRR19973065.2990440/150‑1 (MQ=60)
                                                           GTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCG                                                                                            <  SRR19973065.399271/150‑1 (MQ=60)
                                                                GAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAA                                                                                       <  SRR19973065.3014831/150‑1 (MQ=60)
                                                                 AAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAAC                                                                                      >  SRR19973065.1441823/1‑150 (MQ=60)
                                                                  AGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACC                                                                                     >  SRR19973065.1100454/1‑150 (MQ=60)
                                                                     GCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGG                                                                                  <  SRR19973065.1370739/150‑1 (MQ=60)
                                                                           AGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACC                                                                            <  SRR19973065.467799/150‑1 (MQ=60)
                                                                           AGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACC                                                                            >  SRR19973065.594303/1‑150 (MQ=60)
                                                                           AGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACC                                                                            <  SRR19973065.3079147/150‑1 (MQ=60)
                                                                             TGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTA                                                                          <  SRR19973065.561857/150‑1 (MQ=60)
                                                                              GGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTAC                                                                         >  SRR19973065.1507909/1‑150 (MQ=60)
                                                                              GGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTAC                                                                         >  SRR19973065.235846/1‑150 (MQ=60)
                                                                                  ACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCA                                                                     <  SRR19973065.3452336/150‑1 (MQ=60)
                                                                                   CATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAA                                                                    >  SRR19973065.1973918/1‑150 (MQ=60)
                                                                                       ATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGA                                                                <  SRR19973065.2521447/150‑1 (MQ=60)
                                                                                       ATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGA                                                                <  SRR19973065.2521512/150‑1 (MQ=60)
                                                                                         AAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGT                                                              >  SRR19973065.1044852/1‑150 (MQ=60)
                                                                                              TCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAAC                                                         <  SRR19973065.1801867/150‑1 (MQ=60)
                                                                                              TCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAAC                                                         <  SRR19973065.1801873/150‑1 (MQ=60)
                                                                                               CGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACT                                                        <  SRR19973065.2701067/150‑1 (MQ=60)
                                                                                                 ATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGC                                                      <  SRR19973065.330196/150‑1 (MQ=60)
                                                                                                  TGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCG                                                     >  SRR19973065.2333520/1‑150 (MQ=60)
                                                                                                   GTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGG                                                    >  SRR19973065.3083101/1‑150 (MQ=60)
                                                                                                     GGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCC                                                  <  SRR19973065.3204787/150‑1 (MQ=60)
                                                                                                         AATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCA                                              >  SRR19973065.1409578/1‑150 (MQ=60)
                                                                                                         AATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCA                                              >  SRR19973065.2343486/1‑150 (MQ=60)
                                                                                                         AATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCA                                              >  SRR19973065.3155788/1‑150 (MQ=60)
                                                                                                         AATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCA                                              >  SRR19973065.1895778/1‑150 (MQ=60)
                                                                                                           TCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGG                                            <  SRR19973065.427247/150‑1 (MQ=60)
                                                                                                           TCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGG                                            <  SRR19973065.427173/150‑1 (MQ=60)
                                                                                                            CACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGC                                           <  SRR19973065.1837202/150‑1 (MQ=60)
                                                                                                            CACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGC                                           <  SRR19973065.2135271/150‑1 (MQ=60)
                                                                                                             ACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCT                                          <  SRR19973065.190801/150‑1 (MQ=60)
                                                                                                                   CGGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAG                                    <  SRR19973065.2471809/150‑1 (MQ=60)
                                                                                                                    GGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGC                                   <  SRR19973065.459917/150‑1 (MQ=60)
                                                                                                                    GGATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGC                                   <  SRR19973065.1490949/150‑1 (MQ=60)
                                                                                                                     GATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGATTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCG                                  >  SRR19973065.1935932/1‑150 (MQ=60)
                                                                                                                      ATGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGT                                 >  SRR19973065.3019943/1‑150 (MQ=60)
                                                                                                                       TGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCTTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTT                                <  SRR19973065.737649/150‑1 (MQ=60)
                                                                                                                       TGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTT                                <  SRR19973065.735256/150‑1 (MQ=60)
                                                                                                                       TGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTT                                <  SRR19973065.737611/150‑1 (MQ=60)
                                                                                                                       TGGCATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTT                                <  SRR19973065.841642/150‑1 (MQ=60)
                                                                                                                          CATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCT                             >  SRR19973065.1266384/1‑150 (MQ=60)
                                                                                                                          CATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCT                             >  SRR19973065.2918060/1‑150 (MQ=60)
                                                                                                                          CATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCT                             >  SRR19973065.2540651/1‑150 (MQ=60)
                                                                                                                           ATCCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTG                            >  SRR19973065.318394/1‑150 (MQ=60)
                                                                                                                             CCAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGAT                          >  SRR19973065.3412839/1‑150 (MQ=60)
                                                                                                                              CAGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATC                         <  SRR19973065.8669/150‑1 (MQ=60)
                                                                                                                               AGACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCT                        >  SRR19973065.1555671/1‑150 (MQ=60)
                                                                                                                                GACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTT                       >  SRR19973065.1548591/1‑150 (MQ=60)
                                                                                                                                GACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTT                       >  SRR19973065.871081/1‑150 (MQ=60)
                                                                                                                                 ACCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTC                      >  SRR19973065.2709365/1‑150 (MQ=60)
                                                                                                                                  CCTTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCC                     >  SRR19973065.2460442/1‑150 (MQ=60)
                                                                                                                                    TTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCAC                   <  SRR19973065.2856075/150‑1 (MQ=60)
                                                                                                                                    TTCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCAC                   <  SRR19973065.2161970/150‑1 (MQ=60)
                                                                                                                                     TCCTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACG                  >  SRR19973065.3269923/1‑150 (MQ=60)
                                                                                                                                       CTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCG                >  SRR19973065.3488245/1‑150 (MQ=60)
                                                                                                                                       CTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCG                >  SRR19973065.2511461/1‑150 (MQ=60)
                                                                                                                                       CTGTACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCG                >  SRR19973065.3488249/1‑150 (MQ=60)
                                                                                                                                          TACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACG             <  SRR19973065.716849/150‑1 (MQ=60)
                                                                                                                                          TACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACG             <  SRR19973065.1575818/150‑1 (MQ=60)
                                                                                                                                          TACAGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACG             <  SRR19973065.2888977/150‑1 (MQ=60)
                                                                                                                                             AGTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGC          >  SRR19973065.2502569/1‑150 (MQ=60)
                                                                                                                                              GTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCT         <  SRR19973065.2988470/150‑1 (MQ=60)
                                                                                                                                              GTGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCT         >  SRR19973065.3055522/1‑150 (MQ=60)
                                                                                                                                               TGAAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTG        <  SRR19973065.1683946/150‑1 (MQ=60)
                                                                                                                                                 AAGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCG      <  SRR19973065.294787/150‑1 (MQ=60)
                                                                                                                                                  AGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGT     >  SRR19973065.1003319/1‑150 (MQ=60)
                                                                                                                                                  AGTTAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGT     >  SRR19973065.1653912/1‑150 (MQ=60)
                                                                                                                                                     TAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATT  <  SRR19973065.1139450/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATGCACCGTGCCCAGATGGTTTGCCACTTCCTGGGCTGCTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATT  >  CP009273/693590‑693888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑