Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F309 I0 R1	1697	197.2	6627756	95.7%	6342762	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,325,982	G→T	90.2%	T226T (ACG→ACT)	topA →	DNA topoisomerase I, omega subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,325,982	0	G	T	90.2%	376.4 / 34.9	150	T226T (ACG→ACT)	topA	DNA topoisomerase I, omega subunit
Reads supporting (aligned to +/- strand): ref base G (7/8); new base T (56/79); total (63/87)
Fisher's exact test for biased strand distribution p-value = 7.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

TGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAA  >  CP009273/1325837‑1326126

tGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTAc                                                                                                                                                >  1:1113594/1‑148 (MQ=255)

    ttCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACg                                                                                                                                            >  2:1037131/1‑148 (MQ=255)

    ttCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACg                                                                                                                                            <  1:1851591/148‑1 (MQ=255)

     tCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGc                                                                                                                                           <  2:2784742/148‑1 (MQ=255)

     tCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGc                                                                                                                                           <  2:2784686/148‑1 (MQ=255)

        ccgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCAt                                                                                                                                        >  2:2912298/1‑148 (MQ=255)

         cgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATc                                                                                                                                       <  1:1686021/148‑1 (MQ=255)

           ctgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTg                                                                                                                                     >  1:3018270/1‑148 (MQ=255)

           ctgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTAGCCAGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTg                                                                                                                                     >  1:1399077/1‑148 (MQ=255)

             gctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGt                                                                                                                                   >  1:1794447/1‑148 (MQ=255)

             gctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGt                                                                                                                                   >  1:1865810/1‑148 (MQ=255)

             gctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGt                                                                                                                                   >  1:1794254/1‑148 (MQ=255)

              ctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTg                                                                                                                                  >  2:822864/1‑148 (MQ=255)

                  ggAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGc                                                                                                                              >  2:1180888/1‑148 (MQ=255)

                  ggAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGc                                                                                                                              >  2:1180887/1‑148 (MQ=255)

                  ggAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGc                                                                                                                              >  2:802145/1‑148 (MQ=255)

                   gAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCg                                                                                                                             <  1:3010776/148‑1 (MQ=255)

                       aaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTgg                                                                                                                         <  2:2723314/148‑1 (MQ=255)

                       aaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCGGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTgg                                                                                                                         >  2:565318/1‑148 (MQ=255)

                          aTCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGt                                                                                                                      <  1:1107098/148‑1 (MQ=255)

                          aTCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTATGGGAAGTAGATGCCAGCACTACCACGCCAACTGGTGAAGCGTTGGCGt                                                                                                                      >  1:1791661/1‑148 (MQ=255)

                              cTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACa                                                                                                                  <  2:1295360/148‑1 (MQ=255)

                               tCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAg                                                                                                                 >  1:2201828/1‑148 (MQ=255)

                               tCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAg                                                                                                                 <  2:743875/148‑1 (MQ=255)

                                cGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAgg                                                                                                                <  1:1503117/148‑1 (MQ=255)

                                 gTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGt                                                                                                               <  2:3009405/148‑1 (MQ=255)

                                        gTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACtcat                                                                                                        >  2:1029301/1‑148 (MQ=255)

                                            gCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGa                                                                                                    <  2:775302/148‑1 (MQ=255)

                                             ccGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGaa                                                                                                   <  1:3061314/148‑1 (MQ=255)

                                                gTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGa                                                                                                <  1:666504/148‑1 (MQ=255)

                                                gTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGa                                                                                                >  1:2704262/1‑148 (MQ=255)

                                                 tCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTAGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGAc                                                                                               >  1:2078457/1‑148 (MQ=255)

                                                  cGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACa                                                                                              >  2:2856407/1‑148 (MQ=255)

                                                    tgtgCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACaaa                                                                                            <  2:3301487/148‑1 (MQ=255)

                                                    tgtgCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACaaa                                                                                            <  2:945388/148‑1 (MQ=255)

                                                       gCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAAcgg                                                                                         <  2:2081189/148‑3 (MQ=255)

                                                       gCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAAcgg                                                                                         <  2:2081179/148‑3 (MQ=255)

                                                         aGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGtt                                                                                       <  2:3018270/148‑1 (MQ=255)

                                                         aGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGtt                                                                                       <  2:2858203/148‑1 (MQ=255)

                                                         aGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGtt                                                                                       <  1:1013855/148‑1 (MQ=255)

                                                          gTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtc                                                                                      >  2:438540/1‑148 (MQ=255)

                                                          gTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtc                                                                                      >  2:438761/1‑148 (MQ=255)

                                                             ggTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtccgt                                                                                   <  1:1885148/148‑1 (MQ=255)

                                                                     ttCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaa                                                                           >  2:1950971/1‑148 (MQ=255)

                                                                     ttCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaa                                                                           <  1:438761/148‑1 (MQ=255)

                                                                     ttCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaa                                                                           <  1:2053235/148‑1 (MQ=255)

                                                                        gCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaacaa                                                                        <  2:3072156/148‑1 (MQ=255)

                                                                         ccTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACGGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACaaa                                                                       <  2:1773131/148‑1 (MQ=255)

                                                                            ggtggtCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGaa                                                                    >  1:830693/1‑148 (MQ=255)

                                                                             gtggtCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAAc                                                                   >  2:2604287/1‑148 (MQ=255)

                                                                                gtCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACaaa                                                                <  2:3274885/148‑1 (MQ=255)

                                                                                gtCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACaaa                                                                <  2:2479057/148‑1 (MQ=255)

                                                                                      cgtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAgg                                                          <  1:57925/148‑1 (MQ=255)

                                                                                      cgtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAgg                                                          <  2:1000419/148‑1 (MQ=255)

                                                                                        tgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCt                                                        >  2:3087372/1‑148 (MQ=255)

                                                                                         gagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTg                                                       <  1:2842134/148‑1 (MQ=255)

                                                                                            cgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCgg                                                    >  1:1395639/1‑148 (MQ=255)

                                                                                              tgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTa                                                  <  1:2274276/148‑1 (MQ=255)

                                                                                               gaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTaa                                                 >  1:412995/1‑148 (MQ=255)

                                                                                                aaaTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAg                                                >  2:347355/1‑148 (MQ=255)

                                                                                                aaaTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAg                                                >  2:347348/1‑148 (MQ=255)

                                                                                                aaaTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAg                                                >  1:2712266/1‑148 (MQ=255)

                                                                                                aaaTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAg                                                <  2:520062/148‑1 (MQ=255)

                                                                                                 aaTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGt                                               <  1:762749/148‑1 (MQ=255)

                                                                                                          gTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGaa                                      <  2:97328/148‑1 (MQ=255)

                                                                                                           ttCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTCGTGAAGCTTTGGCGTTACAGGTGACTCATAAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAAAAAACTAAGGCTGCGCTAAGTCTGCTGGaaa                                     >  2:1282001/1‑148 (MQ=255)

                                                                                                            tCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGaaaa                                    >  2:3103981/1‑148 (MQ=255)

                                                                                                            tCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGaaaa                                    <  2:1694691/148‑1 (MQ=255)

                                                                                                            tCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGaaaa                                    >  2:1540450/1‑148 (MQ=255)

                                                                                                             cGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGaaaaa                                   <  1:2977354/148‑1 (MQ=255)

                                                                                                              gTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAg                                  <  2:1794254/148‑1 (MQ=255)

                                                                                                              gTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAg                                  <  2:2943907/148‑1 (MQ=255)

                                                                                                              gTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAg                                  <  2:1865810/148‑1 (MQ=255)

                                                                                                                gCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcg                                <  2:1531738/148‑1 (MQ=255)

                                                                                                                  cGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgcg                              <  1:1029301/148‑1 (MQ=255)

                                                                                                                   ggAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgcgc                             <  1:2231171/148‑1 (MQ=255)

                                                                                                                    gAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCt                            <  1:412847/148‑1 (MQ=255)

                                                                                                                     aaGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTa                           <  2:2704475/148‑1 (MQ=255)

                                                                                                                     aaGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTa                           >  1:796954/1‑148 (MQ=255)

                                                                                                                     aaGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTa                           >  1:2880779/1‑148 (MQ=255)

                                                                                                                      agagTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTAc                          <  2:2540433/148‑1 (MQ=255)

                                                                                                                        agTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAg                        <  1:226672/148‑1 (MQ=255)

                                                                                                                        agTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAg                        <  1:227244/148‑1 (MQ=255)

                                                                                                                          ttCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCGACAGCg                      <  1:1409902/148‑1 (MQ=255)

                                                                                                                           tCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGt                     >  1:1997197/1‑148 (MQ=255)

                                                                                                                            cTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTg                    <  1:3206595/148‑1 (MQ=255)

                                                                                                                               ggAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTg                 >  2:1279714/1‑148 (MQ=255)

                                                                                                                                 aaGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGa               >  1:2568941/1‑148 (MQ=255)

                                                                                                                                  aGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGaa              <  1:802145/148‑1 (MQ=255)

                                                                                                                                   gTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAAc             >  1:2649488/1‑148 (MQ=255)

                                                                                                                                     cGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGt           <  2:2427648/148‑1 (MQ=255)

                                                                                                                                       aTGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGa         <  1:1180888/148‑1 (MQ=255)

                                                                                                                                       aTGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGa         <  1:1180887/148‑1 (MQ=255)

                                                                                                                                         gCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAg       >  2:3139688/1‑148 (MQ=255)

                                                                                                                                         gCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAg       <  1:2942613/148‑1 (MQ=255)

                                                                                                                                         gCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAg       <  1:1295581/148‑1 (MQ=255)

                                                                                                                                         gCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAg       >  2:1719539/1‑148 (MQ=255)

                                                                                                                                          ccAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGa      <  1:1570656/148‑1 (MQ=255)

                                                                                                                                              cACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACaaa  >  1:2328530/1‑148 (MQ=255)

                                                                                                                                              cACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACaaa  >  2:1373034/1‑148 (MQ=255)

TGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAA  >  CP009273/1325837‑1326126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCG  >  CP009273/1325835‑1326129

TATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTAC                                                                                                                                                   >  SRR19973070.1121204/1‑150 (MQ=60)

      TTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCC                                                                                                                                             <  SRR19973070.1864278/150‑1 (MQ=60)

          CCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCT                                                                                                                                         <  SRR19973070.793286/150‑1 (MQ=60)

           CGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTG                                                                                                                                        >  SRR19973070.3037514/1‑150 (MQ=60)

           CGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTG                                                                                                                                        <  SRR19973070.1697587/150‑1 (MQ=60)

           CGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTAGCCAGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTG                                                                                                                                        >  SRR19973070.1408506/1‑150 (MQ=60)

             CTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGT                                                                                                                                      >  SRR19973070.1878556/1‑150 (MQ=60)

             CTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGT                                                                                                                                      >  SRR19973070.1806841/1‑150 (MQ=60)

             CTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGT                                                                                                                                      >  SRR19973070.1806648/1‑150 (MQ=60)

              TGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTG                                                                                                                                     <  SRR19973070.1468558/150‑1 (MQ=60)

                   TGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGGTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCG                                                                                                                                >  SRR19973070.1295655/1‑150 (MQ=60)

                     GAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTT                                                                                                                              <  SRR19973070.3029993/150‑1 (MQ=60)

                     GAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTT                                                                                                                              <  SRR19973070.3029909/150‑1 (MQ=60)

                        AAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCTGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGC                                                                                                                           <  SRR19973070.1684546/150‑1 (MQ=60)

                          AGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTATGGGAAGTAGATGCCAGCACTACCACGCCAACTGGTGAAGCGTTGGCGT                                                                                                                         >  SRR19973070.1804039/1‑150 (MQ=60)

                            ATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTA                                                                                                                       <  SRR19973070.610236/150‑1 (MQ=60)

                            ATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTA                                                                                                                       <  SRR19973070.1114682/150‑1 (MQ=60)

                               GCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAG                                                                                                                    >  SRR19973070.2216338/1‑150 (MQ=60)

                                  CGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTG                                                                                                                 <  SRR19973070.1513640/150‑1 (MQ=60)

                                         TGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGATCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTAGATGCCAGCACTAACACCCCCACTGGTGAATCCTTGGCGTTACAGGTGACTCAaa                                                                                                          >  SRR19973070.2247390/1‑148 (MQ=60)

                                          GTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTAGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTAGTGCCGGAAGATTTATGGGAAGTCGATGCCAGCACTACCACCCCAACTGGTGAAGCGTTGGCGTTACAGGTGAATCATCA                                                                                                         >  SRR19973070.2017056/1‑150 (MQ=60)

                                               CCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACG                                                                                                    <  SRR19973070.3080912/150‑1 (MQ=60)

                                                CGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGA                                                                                                   >  SRR19973070.2721752/1‑150 (MQ=60)

                                                 GGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTAGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGAC                                                                                                  >  SRR19973070.2092447/1‑150 (MQ=60)

                                                  GTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACA                                                                                                 <  SRR19973070.671261/150‑1 (MQ=60)

                                                           AGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCC                                                                                        <  SRR19973070.1021059/150‑1 (MQ=60)

                                                               GGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCC                                                                                    <  SRR19973070.1897845/150‑1 (MQ=60)

                                                               GGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCC                                                                                    <  SRR19973070.1897952/150‑1 (MQ=60)

                                                                   GCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTC                                                                                <  SRR19973070.246259/150‑1 (MQ=60)

                                                                       TTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACA                                                                            <  SRR19973070.441723/150‑1 (MQ=60)

                                                                       TTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACA                                                                            <  SRR19973070.441947/150‑1 (MQ=60)

                                                                       TTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACA                                                                            <  SRR19973070.2067113/150‑1 (MQ=60)

                                                                            CTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAA                                                                       >  SRR19973070.835708/1‑150 (MQ=60)

                                                                            CTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAA                                                                       >  SRR19973070.836328/1‑150 (MQ=60)

                                                                                 GGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAAC                                                                  >  SRR19973070.1053775/1‑150 (MQ=60)

                                                                                 GGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAAC                                                                  >  SRR19973070.2713450/1‑150 (MQ=60)

                                                                                        CGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCT                                                           <  SRR19973070.58596/150‑1 (MQ=60)

                                                                                        CGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCT                                                           <  SRR19973070.365218/150‑1 (MQ=60)

                                                                                           GAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCG                                                        <  SRR19973070.2110902/150‑1 (MQ=60)

                                                                                           GAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCG                                                        <  SRR19973070.1805585/150‑1 (MQ=60)

                                                                                           GAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCG                                                        <  SRR19973070.2860535/150‑1 (MQ=60)

                                                                                            AGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGG                                                       >  SRR19973070.1405053/1‑150 (MQ=60)

                                                                                            AGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGG                                                       >  SRR19973070.1405046/1‑150 (MQ=60)

                                                                                               GTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAA                                                    >  SRR19973070.416006/1‑150 (MQ=60)

                                                                                                TGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAG                                                   <  SRR19973070.2289374/150‑1 (MQ=60)

                                                                                                TGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAG                                                   <  SRR19973070.2289385/150‑1 (MQ=60)

                                                                                                TGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAG                                                   >  SRR19973070.2729796/1‑150 (MQ=60)

                                                                                                   AATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCT                                                <  SRR19973070.768105/150‑1 (MQ=60)

                                                                                                               CGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGC                                    <  SRR19973070.2996466/150‑1 (MQ=60)

                                                                                                               CGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGC                                    <  SRR19973070.3074450/150‑1 (MQ=60)

                                                                                                                    CGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCT                               <  SRR19973070.1036590/150‑1 (MQ=60)

                                                                                                                     GGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTA                              >  SRR19973070.802456/1‑150 (MQ=60)

                                                                                                                     GGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTA                              <  SRR19973070.2245870/150‑1 (MQ=60)

                                                                                                                     GGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTA                              >  SRR19973070.2899429/1‑150 (MQ=60)

                                                                                                                     GGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTA                              >  SRR19973070.1809108/1‑150 (MQ=60)

                                                                                                                      GAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTAC                             <  SRR19973070.415858/150‑1 (MQ=60)

                                                                                                                          AGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCG                         <  SRR19973070.228289/150‑1 (MQ=60)

                                                                                                                          AGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCG                         <  SRR19973070.228863/150‑1 (MQ=60)

                                                                                                                           GTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGT                        >  SRR19973070.2010728/1‑150 (MQ=60)

                                                                                                                            TTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCGACAGCGTG                       <  SRR19973070.1419511/150‑1 (MQ=60)

                                                                                                                              CTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCT                     <  SRR19973070.3227336/150‑1 (MQ=60)

                                                                                                                                 GGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGA                  >  SRR19973070.2585731/1‑150 (MQ=60)

                                                                                                                                   AAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAAC                <  SRR19973070.279406/150‑1 (MQ=60)

                                                                                                                                   AAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAAC                >  SRR19973070.2666731/1‑150 (MQ=60)

                                                                                                                                    AGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACG               <  SRR19973070.807667/150‑1 (MQ=60)

                                                                                                                                    AGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACG               <  SRR19973070.1146093/150‑1 (MQ=60)

                                                                                                                                         ATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAG          <  SRR19973070.1189182/150‑1 (MQ=60)

                                                                                                                                         ATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAG          <  SRR19973070.1189183/150‑1 (MQ=60)

                                                                                                                                           GCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGAC        <  SRR19973070.1304601/150‑1 (MQ=60)

                                                                                                                                           GCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGAC        >  SRR19973070.293504/1‑150 (MQ=60)

                                                                                                                                           GCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGAC        <  SRR19973070.2961576/150‑1 (MQ=60)

                                                                                                                                            CCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACA       <  SRR19973070.1581689/150‑1 (MQ=60)

                                                                                                                                              AGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAA     >  SRR19973070.2344000/1‑150 (MQ=60)

                                                                                                                                                 ACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCG  >  SRR19973070.1003405/1‑150 (MQ=60)

TATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCG  >  CP009273/1325835‑1326129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`