Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,325,982 G→T 100% T226T (ACG→ACT)  topA → DNA topoisomerase I, omega subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,325,9820GT96.4% 571.7 / 11.8 196T226T (ACG→ACT) topADNA topoisomerase I, omega subunit
Reads supporting (aligned to +/- strand):  ref base G (5/2);  new base T (94/94);  total (100/96)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 196 total aligned reads displayed.

TGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAA  >  CP009273/1325837‑1326126
                                                                                                                                                 |                                                                                                                                                
 ggTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTAcc                                                                                                                                               <  2:3335846/148‑1 (MQ=255)
 ggTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTAcc                                                                                                                                               <  1:1544168/148‑1 (MQ=255)
          gctgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCt                                                                                                                                      >  2:3316817/1‑148 (MQ=255)
          gctgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCt                                                                                                                                      <  1:2008539/148‑1 (MQ=255)
              ctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTg                                                                                                                                  <  2:1206903/148‑1 (MQ=255)
                   gAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCg                                                                                                                             <  2:2672315/148‑1 (MQ=255)
                   gAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCg                                                                                                                             <  2:1845120/148‑1 (MQ=255)
                   gAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCg                                                                                                                             <  1:737014/148‑1 (MQ=255)
                   gAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCg                                                                                                                             >  2:723752/1‑148 (MQ=255)
                   gAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCg                                                                                                                             <  1:1723329/148‑1 (MQ=255)
                     aaaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGtt                                                                                                                           >  2:511107/1‑148 (MQ=255)
                     aaaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGtt                                                                                                                           <  2:1718377/148‑1 (MQ=255)
                       aaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTgg                                                                                                                         >  1:1323551/1‑148 (MQ=255)
                         gATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCg                                                                                                                       <  2:2489269/148‑1 (MQ=255)
                          aTCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGt                                                                                                                      <  2:2638809/148‑1 (MQ=255)
                            cGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTa                                                                                                                    <  2:2372199/148‑1 (MQ=255)
                              cTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACa                                                                                                                  <  1:825840/148‑1 (MQ=255)
                               tCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAg                                                                                                                 <  2:447459/148‑1 (MQ=255)
                                 gTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGt                                                                                                               <  1:3271521/148‑1 (MQ=255)
                                 gTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGt                                                                                                               <  1:1282288/148‑1 (MQ=255)
                                    gCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGAc                                                                                                            >  2:3485015/1‑148 (MQ=255)
                                    gCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGAc                                                                                                            >  1:3189513/1‑148 (MQ=255)
                                    gCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCGGGTGGTCGAGCGGGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGCAGTCGATGCCAGCACTACCACCCCAACTGGTGAAGCGTTGGCGTTACAGGTGAc                                                                                                            >  1:2124273/1‑148 (MQ=255)
                                     ccTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACt                                                                                                           >  1:1691304/1‑148 (MQ=255)
                                     ccTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACt                                                                                                           >  1:1952962/1‑148 (MQ=255)
                                     ccTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACt                                                                                                           <  2:2810575/148‑1 (MQ=255)
                                      cTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACtc                                                                                                          <  2:1664686/148‑1 (MQ=255)
                                         tCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACtcatc                                                                                                       <  2:1906345/148‑1 (MQ=255)
                                         tCTGCCGGTCGTGTGCAGTCGGTGGCGGTTAGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTTGGAAGTAGATGCCAGCACAAACACCCCCTCTTGTGAAGCGTTGGCGTTAAACGTGACtcaaa                                                                                                       >  2:1214015/1‑146 (MQ=255)
                                          cTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACtcatca                                                                                                      >  2:1321601/1‑148 (MQ=255)
                                            gCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGa                                                                                                    >  1:2295055/1‑148 (MQ=255)
                                             ccGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGaa                                                                                                   <  2:78541/148‑1 (MQ=255)
                                             ccGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGaa                                                                                                   <  1:2841909/148‑1 (MQ=255)
                                                   gtgtGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACaa                                                                                             <  2:226454/148‑1 (MQ=255)
                                                     gtgCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAAc                                                                                           <  1:3166115/148‑1 (MQ=255)
                                                     gtgCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAAc                                                                                           <  2:2294798/148‑1 (MQ=255)
                                                       gCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCg                                                                                         <  1:1188639/148‑1 (MQ=255)
                                                         aGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGtt                                                                                       <  2:424077/148‑1 (MQ=255)
                                                          gTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtc                                                                                      >  2:77203/1‑148 (MQ=255)
                                                                  cGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGt                                                                              >  2:1072285/1‑148 (MQ=255)
                                                                    gTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTca                                                                            >  2:2432131/1‑148 (MQ=255)
                                                                     ttCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaa                                                                           >  1:403080/1‑148 (MQ=255)
                                                                     ttCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaa                                                                           >  1:2197981/1‑148 (MQ=255)
                                                                     ttCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaa                                                                           >  2:3359262/1‑148 (MQ=255)
                                                                     ttCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaa                                                                           >  1:781454/1‑148 (MQ=255)
                                                                     ttCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaa                                                                           >  2:547318/1‑148 (MQ=255)
                                                                      tCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGGGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaac                                                                          >  1:2981915/1‑148 (MQ=255)
                                                                      tCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaac                                                                          >  1:18450/1‑148 (MQ=255)
                                                                               ggtCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACaa                                                                 >  2:591475/1‑148 (MQ=255)
                                                                                gtCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACaaa                                                                <  2:95598/148‑1 (MQ=255)
                                                                                     gcgtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAg                                                           <  1:2268754/148‑1 (MQ=255)
                                                                                      cgtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAgg                                                          >  1:830703/1‑148 (MQ=255)
                                                                                      cgtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAgg                                                          >  1:1910195/1‑148 (MQ=255)
                                                                                      cgtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAgg                                                          >  1:1913114/1‑148 (MQ=255)
                                                                                      cgtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAgg                                                          <  2:2829841/148‑1 (MQ=255)
                                                                                         gagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTg                                                       >  2:3054397/1‑148 (MQ=255)
                                                                                         gagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTg                                                       <  1:2320215/148‑1 (MQ=255)
                                                                                         gagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTg                                                       <  1:2888228/148‑1 (MQ=255)
                                                                                          agcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGc                                                      <  2:640066/148‑1 (MQ=255)
                                                                                              tgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTa                                                  <  1:511107/148‑1 (MQ=255)
                                                                                              tgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTa                                                  <  2:1793591/148‑1 (MQ=255)
                                                                                              tgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTa                                                  >  2:2006917/1‑148 (MQ=255)
                                                                                                  aTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTc                                              <  2:3227558/148‑1 (MQ=255)
                                                                                                  aTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTc                                              >  2:2366434/1‑148 (MQ=255)
                                                                                                    tAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctg                                            <  1:490884/148‑1 (MQ=255)
                                                                                                    tAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctg                                            >  2:2470390/1‑148 (MQ=255)
                                                                                                      aaGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctgct                                          <  1:1071115/148‑1 (MQ=255)
                                                                                                      aaGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctgct                                          >  1:3242613/1‑148 (MQ=255)
                                                                                                       aGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctgctg                                         >  1:1473912/1‑148 (MQ=255)
                                                                                                         cGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGa                                       >  2:6617/1‑148 (MQ=255)
                                                                                                         cGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGACAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGa                                       >  2:152659/1‑148 (MQ=255)
                                                                                                         cGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTAATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGa                                       >  1:2949951/1‑148 (MQ=255)
                                                                                                            tCGTGCCGGAAGAGTTCTGTGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGaaaa                                    <  1:1671888/148‑1 (MQ=255)
                                                                                                             cGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGaaaaa                                   <  1:77203/148‑1 (MQ=255)
                                                                                                                   ggAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgcgc                             >  1:1008644/1‑148 (MQ=255)
                                                                                                                   ggAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgcgc                             >  1:1008302/1‑148 (MQ=255)
                                                                                                                    gAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCt                            >  1:3066107/1‑148 (MQ=255)
                                                                                                                     aaGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCGGGAAAAAGCGCGCTa                           >  2:855621/1‑148 (MQ=255)
                                                                                                                      agagTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTAc                          >  1:2431584/1‑148 (MQ=255)
                                                                                                                       gagTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACa                         <  2:2116056/148‑1 (MQ=255)
                                                                                                                       gagTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACa                         >  1:481727/1‑148 (MQ=255)
                                                                                                                          ttCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCg                      <  2:1690693/148‑1 (MQ=255)
                                                                                                                          ttCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCg                      >  2:1403043/1‑148 (MQ=255)
                                                                                                                          ttCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCg                      <  2:1952962/148‑1 (MQ=255)
                                                                                                                          ttCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCg                      <  1:2770662/148‑1 (MQ=255)
                                                                                                                          ttCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCg                      <  2:1691441/148‑1 (MQ=255)
                                                                                                                           tCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGt                     >  2:892370/1‑148 (MQ=255)
                                                                                                                            cTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTg                    <  1:3118825/148‑1 (MQ=255)
                                                                                                                             tGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGc                   <  2:2386367/148‑1 (MQ=255)
                                                                                                                                gAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTgg                >  2:3540251/1‑148 (MQ=255)
                                                                                                                                 aaGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGa               <  2:3082507/148‑1 (MQ=255)
                                                                                                                                 aaGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGa               <  2:1308111/148‑1 (MQ=255)
                                                                                                                                  aGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGaa              <  1:1701822/148‑1 (MQ=255)
                                                                                                                                       aTGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAATAGCGCGCTACAGCGTGCTGGAACGTGa         >  2:474116/1‑148 (MQ=255)
                                                                                                                                       aTGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGa         >  2:3149811/1‑148 (MQ=255)
                                                                                                                                       aTGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGa         >  2:1555850/1‑148 (MQ=255)
                                                                                                                                         gCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAg       <  1:2838674/148‑1 (MQ=255)
                                                                                                                                         gCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAg       <  1:2838146/148‑1 (MQ=255)
                                                                                                                                         gCCAGCACGACCACTCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAg       >  1:997285/1‑148 (MQ=255)
                                                                                                                                            aGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACa    >  1:53127/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAA  >  CP009273/1325837‑1326126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACA  >  CP009273/1325834‑1326124
                                                                                                                                                    |                                                                                                                                              
ATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTA                                                                                                                                               >  SRR19973066.2848857/1‑150 (MQ=60)
ATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTA                                                                                                                                               >  SRR19973066.2849566/1‑150 (MQ=60)
ATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTA                                                                                                                                               >  SRR19973066.1769203/1‑150 (MQ=60)
 TATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTAC                                                                                                                                              >  SRR19973066.2310530/1‑150 (MQ=60)
    GGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCAC                                                                                                                                           <  SRR19973066.2324685/150‑1 (MQ=60)
    GGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCAC                                                                                                                                           <  SRR19973066.1555219/150‑1 (MQ=60)
             GCTGCTATGGAAATAGTTCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGG                                                                                                                                  <  SRR19973066.2022620/150‑1 (MQ=60)
             GCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGG                                                                                                                                  <  SRR19973066.2022623/150‑1 (MQ=60)
               TGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTG                                                                                                                                >  SRR19973066.3219902/1‑150 (MQ=60)
               TGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTG                                                                                                                                <  SRR19973066.2347940/150‑1 (MQ=60)
                 CTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAA                                                                                                                              <  SRR19973066.3121145/150‑1 (MQ=60)
                      GAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTT                                                                                                                         <  SRR19973066.742484/150‑1 (MQ=60)
                      GAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTT                                                                                                                         <  SRR19973066.1735801/150‑1 (MQ=60)
                        AAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCCGCACTACCACGCCATCTGGTGAAGCGTTTG                                                                                                                       >  SRR19973066.1332526/1‑150 (MQ=60)
                        AAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGG                                                                                                                       >  SRR19973066.1333144/1‑150 (MQ=60)
                                 CTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGG                                                                                                              <  SRR19973066.831905/150‑1 (MQ=60)
                                 CTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGG                                                                                                              <  SRR19973066.831863/150‑1 (MQ=60)
                                    GTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGA                                                                                                           <  SRR19973066.3292996/150‑1 (MQ=60)
                                    GTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGA                                                                                                           <  SRR19973066.1291553/150‑1 (MQ=60)
                                     TTGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGAC                                                                                                          <  SRR19973066.521419/150‑1 (MQ=60)
                                     TGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGAC                                                                                                          >  SRR19973066.3210570/1‑150 (MQ=60)
                                     TGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGAC                                                                                                          >  SRR19973066.3210716/1‑150 (MQ=60)
                                     TGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCGGGTGGTCGAGCGGGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGCAGTCGATGCCAGCACTACCACCCCAACTGGTGAAGCGTTGGCGTTACAGGTGAC                                                                                                          >  SRR19973066.2138983/1‑150 (MQ=60)
                                      GGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGGAGTCGATGCCAGCACTACCACCCCATCTGGTGAAGCGTTGGCGTTACAGGTGACT                                                                                                         >  SRR19973066.1703743/1‑150 (MQ=60)
                                      GGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACT                                                                                                         >  SRR19973066.1966786/1‑150 (MQ=60)
                                      GGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCAGGTGAAGCGTTGGCGTTACAGGTGACT                                                                                                         >  SRR19973066.1703605/1‑150 (MQ=60)
                                      GGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCAGGTGGTCGAGCGTGAGCGTGAAAATAAAGCGTTAGTGACCGAAGAGTTCTGGGAAGTAGATTCCACCACTAACACGCCAAAAGGTGAAGCGTTTGCGTTACAGGTGACT                                                                                                         >  SRR19973066.1702994/1‑150 (MQ=60)
                                          TGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATC                                                                                                     <  SRR19973066.421078/150‑1 (MQ=60)
                                          TGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATC                                                                                                     >  SRR19973066.2276684/1‑150 (MQ=60)
                                             CTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGA                                                                                                  >  SRR19973066.2310788/1‑150 (MQ=60)
                                                CCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACG                                                                                               <  SRR19973066.2861007/150‑1 (MQ=60)
                                                   GTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACA                                                                                            <  SRR19973066.2685681/150‑1 (MQ=60)
                                                   GTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACA                                                                                            <  SRR19973066.830521/150‑1 (MQ=60)
                                                      GTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAAC                                                                                         <  SRR19973066.1542739/150‑1 (MQ=60)
                                                        GTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCG                                                                                       <  SRR19973066.1614812/150‑1 (MQ=60)
                                                        GTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCG                                                                                       <  SRR19973066.3187205/150‑1 (MQ=60)
                                                         TGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGT                                                                                      >  SRR19973066.2854354/1‑150 (MQ=60)
                                                          GCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTT                                                                                     <  SRR19973066.1196975/150‑1 (MQ=60)
                                                               CGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTC                                                                                >  SRR19973066.2159265/1‑150 (MQ=60)
                                                                  TGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGagatcggaagagcacacgtctgaactccagtcactaccaatg                                                                             >  SRR19973066.2077340/1‑108 (MQ=60)
                                                                      GGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAA                                                                         >  SRR19973066.406255/1‑150 (MQ=60)
                                                                      GGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAA                                                                         >  SRR19973066.2213222/1‑150 (MQ=60)
                                                                      GGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAA                                                                         >  SRR19973066.787220/1‑150 (MQ=60)
                                                                       GTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGGGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAAC                                                                        >  SRR19973066.3001606/1‑150 (MQ=60)
                                                                       GTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAAC                                                                        >  SRR19973066.18728/1‑150 (MQ=60)
                                                                        TTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACA                                                                       <  SRR19973066.296181/150‑1 (MQ=60)
                                                                             CTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCATAACGACAAACCGTTCCGTCCGGTCAACAAAGAA                                                                  >  SRR19973066.3018498/1‑150 (MQ=60)
                                                                                 TGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAA                                                              <  SRR19973066.782051/150‑1 (MQ=60)
                                                                                       AGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGG                                                        >  SRR19973066.1926739/1‑150 (MQ=60)
                                                                                       AGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGG                                                        >  SRR19973066.49293/1‑150 (MQ=60)
                                                                                       AGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGG                                                        >  SRR19973066.1923799/1‑150 (MQ=60)
                                                                                       AGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGG                                                        >  SRR19973066.836762/1‑150 (MQ=60)
                                                                                       AGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGGCCGGTCAACAACGAACAAACTCAGG                                                        >  SRR19973066.1926547/1‑150 (MQ=60)
                                                                                        GCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGC                                                       <  SRR19973066.2284357/150‑1 (MQ=60)
                                                                                          GTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTG                                                     >  SRR19973066.1797012/1‑150 (MQ=60)
                                                                                            GAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCG                                                   <  SRR19973066.2907547/150‑1 (MQ=60)
                                                                                            GAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCG                                                   <  SRR19973066.2336070/150‑1 (MQ=60)
                                                                                                GTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAA                                               >  SRR19973066.3288163/1‑150 (MQ=60)
                                                                                                 TGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAG                                              <  SRR19973066.514887/150‑1 (MQ=60)
                                                                                                 TGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAG                                              <  SRR19973066.514827/150‑1 (MQ=60)
                                                                                                     ATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTG                                          >  SRR19973066.2865006/1‑150 (MQ=60)
                                                                                                       TAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCT                                        <  SRR19973066.494561/150‑1 (MQ=60)
                                                                                                       TAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCT                                        >  SRR19973066.3264002/1‑150 (MQ=60)
                                                                                                        AAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTG                                       >  SRR19973066.1484295/1‑150 (MQ=60)
                                                                                                        AAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTG                                       >  SRR19973066.1484259/1‑150 (MQ=60)
                                                                                                         AAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGG                                      <  SRR19973066.1077561/150‑1 (MQ=60)
                                                                                                         AAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGG                                      <  SRR19973066.1078968/150‑1 (MQ=60)
                                                                                                          AGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTAATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGA                                     >  SRR19973066.2969525/1‑150 (MQ=60)
                                                                                                          AGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTAATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGA                                     >  SRR19973066.2969533/1‑150 (MQ=60)
                                                                                                               TCGTGCCGGAAGAGTTCTGTGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAG                                <  SRR19973066.1684071/150‑1 (MQ=60)
                                                                                                                CGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGC                               <  SRR19973066.78108/150‑1 (MQ=60)
                                                                                                                  TGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGC                             <  SRR19973066.540635/150‑1 (MQ=60)
                                                                                                                   GCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCG                            <  SRR19973066.899842/150‑1 (MQ=60)
                                                                                                                    CCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGC                           >  SRR19973066.1015726/1‑150 (MQ=60)
                                                                                                                    CCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGC                           >  SRR19973066.1016071/1‑150 (MQ=60)
                                                                                                                     CGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCT                          <  SRR19973066.3154872/150‑1 (MQ=60)
                                                                                                                     CGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCT                          >  SRR19973066.3086611/1‑150 (MQ=60)
                                                                                                                       GAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTAC                        >  SRR19973066.2448133/1‑150 (MQ=60)
                                                                                                                        AAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACA                       >  SRR19973066.485348/1‑150 (MQ=60)
                                                                                                                        AAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACA                       >  SRR19973066.1163047/1‑150 (MQ=60)
                                                                                                                             TTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTG                  <  SRR19973066.2789318/150‑1 (MQ=60)
                                                                                                                               CTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCT                <  SRR19973066.3139652/150‑1 (MQ=60)
                                                                                                                                TGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTG               >  SRR19973066.3551045/1‑150 (MQ=60)
                                                                                                                                TGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTG               >  SRR19973066.76298/1‑150 (MQ=60)
                                                                                                                                 GGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGG              >  SRR19973066.1930667/1‑150 (MQ=60)
                                                                                                                                     AGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACG          >  SRR19973066.1895411/1‑150 (MQ=60)
                                                                                                                                     AGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACG          <  SRR19973066.1714183/150‑1 (MQ=60)
                                                                                                                                         GATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAA      <  SRR19973066.527203/150‑1 (MQ=60)
                                                                                                                                          ATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAG     <  SRR19973066.172376/150‑1 (MQ=60)
                                                                                                                                          ATGCCAGCACGACCACTCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAG     >  SRR19973066.1004632/1‑150 (MQ=60)
                                                                                                                                            GCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGAC   <  SRR19973066.2857225/150‑1 (MQ=60)
                                                                                                                                            GCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGAC   <  SRR19973066.2857715/150‑1 (MQ=60)
                                                                                                                                            GCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGAC   <  SRR19973066.2857759/150‑1 (MQ=60)
                                                                                                                                            GCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCGACAGCGTGCTGGAACGTGAAGAC   <  SRR19973066.1222547/150‑1 (MQ=60)
                                                                                                                                             CCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACA  >  SRR19973066.53799/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                              
ATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACA  >  CP009273/1325834‑1326124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑