Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F534 I1 R1	1235	230.2	7611948	95.1%	7238962	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	1,325,982	G→T	T226T (ACG→ACT)	topA →	DNA topoisomerase I, omega subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,325,982	0	G	T	100.0%	475.8 / NA	146	T226T (ACG→ACT)	topA	DNA topoisomerase I, omega subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (79/67); total (79/67)

Only 100 of 146 total aligned reads displayed.

ATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAA  >  CP009273/1325836‑1326126

  ggTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCAc                                                                                                                                             >  2:1267651/1‑150 (MQ=255)

  ggTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCAc                                                                                                                                             >  1:25623/1‑150 (MQ=255)

      tCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCa                                                                                                                                         <  2:1492984/150‑1 (MQ=255)

       cgccgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCAt                                                                                                                                        >  1:593191/1‑150 (MQ=255)

         ccgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCt                                                                                                                                      <  2:1848283/150‑1 (MQ=255)

           gctgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTgg                                                                                                                                    <  1:3499791/150‑1 (MQ=255)

           gctgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTgg                                                                                                                                    >  2:1185448/1‑150 (MQ=255)

            ctgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGt                                                                                                                                   <  2:192795/150‑1 (MQ=255)

            ctgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGt                                                                                                                                   >  1:1092261/1‑150 (MQ=255)

              gctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGa                                                                                                                                 <  2:2135144/150‑1 (MQ=255)

               ctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGaa                                                                                                                                <  2:2259068/150‑1 (MQ=255)

               ctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGaa                                                                                                                                <  2:2258946/150‑1 (MQ=255)

                tATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAg                                                                                                                               >  1:1117549/1‑150 (MQ=255)

                   ggAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGt                                                                                                                            <  2:2751124/150‑1 (MQ=255)

                     aaaaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTg                                                                                                                          >  2:609244/1‑150 (MQ=255)

                      aaaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTgg                                                                                                                         >  2:3508801/1‑150 (MQ=255)

                      aaaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTgg                                                                                                                         >  2:3508728/1‑150 (MQ=255)

                        aaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCg                                                                                                                       >  1:3287529/1‑150 (MQ=255)

                          gATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGtt                                                                                                                     <  1:3153389/150‑1 (MQ=255)

                          gATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGtt                                                                                                                     >  2:1355460/1‑150 (MQ=255)

                          gATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGtt                                                                                                                     >  2:347028/1‑150 (MQ=255)

                             cGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACa                                                                                                                  >  1:3525051/1‑150 (MQ=255)

                             cGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACa                                                                                                                  >  2:352292/1‑150 (MQ=255)

                             cGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACa                                                                                                                  >  2:1174563/1‑150 (MQ=255)

                             cGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACa                                                                                                                  >  2:353005/1‑150 (MQ=255)

                              gCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAg                                                                                                                 >  1:1782443/1‑150 (MQ=255)

                               cTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAgg                                                                                                                >  2:1078921/1‑150 (MQ=255)

                               cTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAgg                                                                                                                <  1:2108013/150‑1 (MQ=255)

                                  gTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTTAAGAGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACTCCATCTGGTGAAGCGTTGGCGTTACAGGTGa                                                                                                             <  2:741453/150‑1 (MQ=255)

                                      ccTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACtca                                                                                                         >  1:1498940/1‑150 (MQ=255)

                                      ccTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACtca                                                                                                         >  1:1498922/1‑150 (MQ=255)

                                          tCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAg                                                                                                     <  2:1908737/150‑1 (MQ=255)

                                            tGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGaa                                                                                                   >  1:101873/1‑150 (MQ=255)

                                             gCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAAc                                                                                                  >  2:2015783/1‑150 (MQ=255)

                                              ccGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACg                                                                                                 >  1:845040/1‑150 (MQ=255)

                                                  tCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACaa                                                                                             <  2:2454389/150‑1 (MQ=255)

                                                    gtgtGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAAc                                                                                           >  1:2794671/1‑150 (MQ=255)

                                                    gtgtGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAAc                                                                                           >  2:1071220/1‑150 (MQ=255)

                                                       tgCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGt                                                                                        <  2:770333/150‑1 (MQ=255)

                                                        gCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGtt                                                                                       <  1:131041/150‑1 (MQ=255)

                                                         cAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtc                                                                                      <  2:1514953/150‑1 (MQ=255)

                                                          aGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtcc                                                                                     <  1:3319448/150‑1 (MQ=255)

                                                          aGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtcc                                                                                     <  2:1895908/150‑1 (MQ=255)

                                                          aGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtcc                                                                                     <  2:1065385/150‑1 (MQ=255)

                                                          aGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtcc                                                                                     <  1:213524/150‑1 (MQ=255)

                                                          aGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtcc                                                                                     <  1:213479/150‑1 (MQ=255)

                                                              ggTGGCGGTTCGCCTGGTGGTCGAGCGTGATCGTGAAATTAAAGCGTTCGTGCAGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtccgtcc                                                                                 >  1:3285571/1‑150 (MQ=255)

                                                               gTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtccgtccg                                                                                <  2:1457487/150‑1 (MQ=255)

                                                                tGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCgg                                                                               >  1:3512898/1‑150 (MQ=255)

                                                                   cGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTca                                                                            <  2:967706/150‑1 (MQ=255)

                                                                   cGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTca                                                                            <  1:2897151/150‑1 (MQ=255)

                                                                    ggTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaa                                                                           >  2:3184870/1‑150 (MQ=255)

                                                                     gTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaac                                                                          <  2:3203435/150‑1 (MQ=255)

                                                                          ccTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGa                                                                     <  1:2918673/150‑1 (MQ=255)

                                                                           cTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGaa                                                                    >  1:2547800/1‑150 (MQ=255)

                                                                           cTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGaa                                                                    >  1:2801801/1‑150 (MQ=255)

                                                                            tggtggTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAAc                                                                   >  1:1002450/1‑150 (MQ=255)

                                                                                ggtCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAAc                                                               >  2:2692487/1‑150 (MQ=255)

                                                                                ggtCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAAc                                                               <  1:2223153/150‑1 (MQ=255)

                                                                                 gtCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACt                                                              <  2:101873/150‑1 (MQ=255)

                                                                                   cGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCa                                                            >  2:897948/1‑150 (MQ=255)

                                                                                       cgtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCt                                                        >  1:3037284/1‑150 (MQ=255)

                                                                                       cgtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCt                                                        >  1:3037451/1‑150 (MQ=255)

                                                                                       cgtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCt                                                        >  1:2523755/1‑150 (MQ=255)

                                                                                       cgtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCt                                                        <  2:2527286/150‑1 (MQ=255)

                                                                                              gtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTaa                                                 >  2:3065116/1‑150 (MQ=255)

                                                                                               tgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAg                                                >  2:1552135/1‑150 (MQ=255)

                                                                                                  aaTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTct                                             <  1:2665791/150‑1 (MQ=255)

                                                                                                    ttAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctgc                                           <  2:951679/150‑1 (MQ=255)

                                                                                                      aaaGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctgctg                                         >  1:2608049/1‑150 (MQ=255)

                                                                                                         gCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGaa                                      <  2:2604208/150‑1 (MQ=255)

                                                                                                          cggtCGTGCCGGACGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCAGGTGAAGCGTTGGCGTTACGGGTGACTCATCAGACCGACCCACCGGTCCGGCCGGTCCACACAGAACAACCTCAGGCGGCGGTCCGTCTGCGGGaca                                     >  2:2525535/4‑148 (MQ=255)

                                                                                                           gTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGaaaa                                    >  1:3002094/1‑150 (MQ=255)

                                                                                                           gTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGaaaa                                    >  1:593105/1‑150 (MQ=255)

                                                                                                             tCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAg                                  <  1:1980980/150‑1 (MQ=255)

                                                                                                             tCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAg                                  <  1:154970/150‑1 (MQ=255)

                                                                                                             tCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAg                                  <  1:1536646/150‑1 (MQ=255)

                                                                                                              cGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgc                                 <  1:1267775/150‑1 (MQ=255)

                                                                                                               gTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcg                                <  2:363359/150‑1 (MQ=255)

                                                                                                                tGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgc                               <  2:523805/150‑1 (MQ=255)

                                                                                                                 gCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgcg                              <  2:12772/150‑1 (MQ=255)

                                                                                                                 gCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgcg                              <  1:1267651/150‑1 (MQ=255)

                                                                                                                 gCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgcg                              <  1:1355460/150‑1 (MQ=255)

                                                                                                                   cGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCt                            >  1:1052504/1‑150 (MQ=255)

                                                                                                                    ggAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTa                           >  2:2412313/1‑150 (MQ=255)

                                                                                                                      aaGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACa                         <  1:1174563/150‑1 (MQ=255)

                                                                                                                       agagTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAg                        <  1:100709/150‑1 (MQ=255)

                                                                                                                       agagTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAg                        >  2:860086/1‑150 (MQ=255)

                                                                                                                           ttCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTg                    <  1:3456267/150‑1 (MQ=255)

                                                                                                                           ttCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGGTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTg                    <  1:3456063/150‑1 (MQ=255)

                                                                                                                             cTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCt                  >  1:2266284/1‑150 (MQ=255)

                                                                                                                              tGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTg                 >  2:754771/1‑150 (MQ=255)

                                                                                                                               gggAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTgg                >  1:1560924/1‑150 (MQ=255)

                                                                                                                                  aaGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAAc             >  1:3143739/1‑150 (MQ=255)

                                                                                                                                    gTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGt           >  1:2072097/1‑150 (MQ=255)

                                                                                                                                    gTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGt           <  1:2955034/150‑1 (MQ=255)

                                                                                                                                      cGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGa         >  2:3788281/1‑150 (MQ=255)

                                                                                                                                        aTGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCGACAGCGTGCTGGAACGTGAAg       <  1:3503085/150‑1 (MQ=255)

                                                                                                                                           ccAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACa    >  2:688519/1‑150 (MQ=255)

                                                                                                                                            cAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACaa   >  2:387653/1‑150 (MQ=255)

ATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAA  >  CP009273/1325836‑1326126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGAC  >  CP009273/1325836‑1326131

ATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACC                                                                                                                                                    >  SRR19973061.1425703/1‑150 (MQ=60)

  GGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCAC                                                                                                                                                  >  SRR19973061.25720/1‑150 (MQ=60)

       CGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCAT                                                                                                                                             >  SRR19973061.594738/1‑150 (MQ=60)

         CCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCT                                                                                                                                           <  SRR19973061.2390359/150‑1 (MQ=60)

         CCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCT                                                                                                                                           <  SRR19973061.1545687/150‑1 (MQ=60)

          CGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTG                                                                                                                                          <  SRR19973061.2155955/150‑1 (MQ=60)

           GCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGG                                                                                                                                         <  SRR19973061.3508736/150‑1 (MQ=60)

            CTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGT                                                                                                                                        >  SRR19973061.1095061/1‑150 (MQ=60)

               CTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAA                                                                                                                                     >  SRR19973061.2610899/1‑150 (MQ=60)

                TATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAG                                                                                                                                    >  SRR19973061.1120407/1‑150 (MQ=60)

                        AAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCG                                                                                                                            >  SRR19973061.3295827/1‑150 (MQ=60)

                        AAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCG                                                                                                                            >  SRR19973061.3295997/1‑150 (MQ=60)

                          GATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTT                                                                                                                          <  SRR19973061.3161495/150‑1 (MQ=60)

                             CGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACA                                                                                                                       >  SRR19973061.3534080/1‑150 (MQ=60)

                              GCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAG                                                                                                                      >  SRR19973061.1786910/1‑150 (MQ=60)

                               CTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGG                                                                                                                     <  SRR19973061.2113306/150‑1 (MQ=60)

                                      CCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCA                                                                                                              >  SRR19973061.1502676/1‑150 (MQ=60)

                                      CCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCA                                                                                                              >  SRR19973061.1502694/1‑150 (MQ=60)

                                       CTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCAT                                                                                                             >  SRR19973061.1631288/1‑150 (MQ=60)

                                            TGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAA                                                                                                        >  SRR19973061.102212/1‑150 (MQ=60)

                                              CCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACG                                                                                                      >  SRR19973061.847244/1‑150 (MQ=60)

                                                    GTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAAC                                                                                                >  SRR19973061.2801850/1‑150 (MQ=60)

                                                        GCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTT                                                                                            <  SRR19973061.131462/150‑1 (MQ=60)

                                                         CAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTC                                                                                           >  SRR19973061.1238196/1‑150 (MQ=60)

                                                          AGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCC                                                                                          <  SRR19973061.3328006/150‑1 (MQ=60)

                                                          AGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCC                                                                                          <  SRR19973061.214156/150‑1 (MQ=60)

                                                          AGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCC                                                                                          <  SRR19973061.214111/150‑1 (MQ=60)

                                                           GTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCG                                                                                         <  SRR19973061.1273283/150‑1 (MQ=60)

                                                              GGTGGCGGTTCGCCTGGTGGTCGAGCGTGATCGTGAAATTAAAGCGTTCGTGCAGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCC                                                                                      >  SRR19973061.3294034/1‑150 (MQ=60)

                                                              GGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCC                                                                                      <  SRR19973061.797281/150‑1 (MQ=60)

                                                                TGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGG                                                                                    >  SRR19973061.3521891/1‑150 (MQ=60)

                                                                   CGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCA                                                                                 <  SRR19973061.2904585/150‑1 (MQ=60)

                                                                   CGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCA                                                                                 <  SRR19973061.2017139/150‑1 (MQ=60)

                                                                     GTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAAC                                                                               >  SRR19973061.3520939/1‑150 (MQ=60)

                                                                          CCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGA                                                                          <  SRR19973061.2926166/150‑1 (MQ=60)

                                                                           CTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAA                                                                         <  SRR19973061.2832224/150‑1 (MQ=60)

                                                                           CTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAA                                                                         >  SRR19973061.2809000/1‑150 (MQ=60)

                                                                           CTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAA                                                                         >  SRR19973061.2554280/1‑150 (MQ=60)

                                                                            TGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAAC                                                                        >  SRR19973061.1005002/1‑150 (MQ=60)

                                                                                GGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAAC                                                                    <  SRR19973061.2228731/150‑1 (MQ=60)

                                                                                 GTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACT                                                                   >  SRR19973061.1052362/1‑150 (MQ=60)

                                                                                    GAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAG                                                                <  SRR19973061.2928753/150‑1 (MQ=60)

                                                                                       CGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCT                                                             >  SRR19973061.2530178/1‑150 (MQ=60)

                                                                                       CGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCT                                                             >  SRR19973061.3045302/1‑150 (MQ=60)

                                                                                       CGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCT                                                             >  SRR19973061.3045135/1‑150 (MQ=60)

                                                                                         TGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGC                                                           >  SRR19973061.2718760/1‑150 (MQ=60)

                                                                                         TGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGC                                                           >  SRR19973061.1098080/1‑150 (MQ=60)

                                                                                              GTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAA                                                      <  SRR19973061.364309/150‑1 (MQ=60)

                                                                                                  AATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCT                                                  <  SRR19973061.2672646/150‑1 (MQ=60)

                                                                                                  AATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCT                                                  >  SRR19973061.3447834/1‑150 (MQ=60)

                                                                                                   ATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTG                                                 >  SRR19973061.2120889/1‑150 (MQ=60)

                                                                                                   ATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTG                                                 >  SRR19973061.3058242/1‑150 (MQ=60)

                                                                                                      AAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTG                                              >  SRR19973061.2614753/1‑150 (MQ=60)

                                                                                                           GTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAA                                         >  SRR19973061.594652/1‑150 (MQ=60)

                                                                                                           GTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAA                                         >  SRR19973061.3009893/1‑150 (MQ=60)

                                                                                                           GTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAA                                         >  SRR19973061.3009842/1‑150 (MQ=60)

                                                                                                             TCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAG                                       <  SRR19973061.155455/150‑1 (MQ=60)

                                                                                                             TCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAG                                       <  SRR19973061.1540474/150‑1 (MQ=60)

                                                                                                             TCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAG                                       <  SRR19973061.1985936/150‑1 (MQ=60)

                                                                                                              CGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGC                                      <  SRR19973061.1270904/150‑1 (MQ=60)

                                                                                                                 GCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCG                                   <  SRR19973061.1358869/150‑1 (MQ=60)

                                                                                                                 GCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCG                                   <  SRR19973061.1270779/150‑1 (MQ=60)

                                                                                                                   CGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCT                                 >  SRR19973061.1055212/1‑150 (MQ=60)

                                                                                                                      AAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACA                              <  SRR19973061.1177537/150‑1 (MQ=60)

                                                                                                                       AGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAG                             <  SRR19973061.101041/150‑1 (MQ=60)

                                                                                                                           TTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTG                         <  SRR19973061.3465132/150‑1 (MQ=60)

                                                                                                                           TTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGGTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTG                         <  SRR19973061.3464928/150‑1 (MQ=60)

                                                                                                                             CTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCT                       >  SRR19973061.2272003/1‑150 (MQ=60)

                                                                                                                               GGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGG                     >  SRR19973061.3467424/1‑150 (MQ=60)

                                                                                                                               GGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGG                     >  SRR19973061.1564803/1‑150 (MQ=60)

                                                                                                                                  AAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAAC                  >  SRR19973061.3151828/1‑150 (MQ=60)

                                                                                                                                    GTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGT                <  SRR19973061.2962656/150‑1 (MQ=60)

                                                                                                                                    GTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGT                >  SRR19973061.2077301/1‑150 (MQ=60)

                                                                                                                                        ATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCGACAGCGTGCTGGAACGTGAAG            <  SRR19973061.3512045/150‑1 (MQ=60)

                                                                                                                                                  TACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGAC  >  SRR19973061.1154221/1‑150 (MQ=60)

ATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGAC  >  CP009273/1325836‑1326131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`