Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F247 I0 R1	1496	299.8	9582018	97.8%	9371213	149.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,325,982	G→T	86.6%	T226T (ACG→ACT)	topA →	DNA topoisomerase I, omega subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,325,982	0	G	T	86.6%	540.3 / 85.6	241	T226T (ACG→ACT)	topA	DNA topoisomerase I, omega subunit
Reads supporting (aligned to +/- strand): ref base G (15/18); new base T (88/115); total (108/133)
Fisher's exact test for biased strand distribution p-value = 8.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 241 total aligned reads displayed.

TGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAAC  >  CP009273/1325837‑1326127

tGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTAcc                                                                                                                                                <  1:3526027/149‑1 (MQ=255)

tGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTAcc                                                                                                                                                >  1:3529227/1‑149 (MQ=255)

 ggTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCa                                                                                                                                               >  2:2546175/1‑149 (MQ=255)

 ggTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCa                                                                                                                                               <  1:3455281/149‑1 (MQ=255)

 ggTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCa                                                                                                                                               >  2:2184814/1‑149 (MQ=255)

 ggTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCa                                                                                                                                               >  2:2184662/1‑149 (MQ=255)

 ggTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCa                                                                                                                                               <  2:4341651/149‑1 (MQ=255)

  gTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCAc                                                                                                                                              >  1:2873302/1‑149 (MQ=255)

     tCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGGGAGCGTGAAATTAAATCGTTCGTGCCGGAAGAGTTCGGGGAAGTCGATGCCAGCACGACCAcccc                                                                                                                                           >  1:1616705/1‑149 (MQ=255)

       gccgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACAACCACGCCaa                                                                                                                                         >  1:2884626/1‑148 (MQ=255)

        ccgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATc                                                                                                                                        >  2:1540394/1‑149 (MQ=255)

        ccgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATc                                                                                                                                        <  2:1126798/149‑1 (MQ=255)

         cgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGGGAAATTAAAGCGTTAGTGCCGGAAGAGTTCTGGGAAGTCGATGCCCGCACAACCACGCCATCt                                                                                                                                       >  1:4423815/1‑149 (MQ=255)

           ctgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTgg                                                                                                                                     <  2:649635/149‑1 (MQ=255)

           ctgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTgg                                                                                                                                     <  2:3708542/149‑1 (MQ=255)

                 tGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGc                                                                                                                               <  2:4016743/149‑1 (MQ=255)

                   gAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGt                                                                                                                             <  2:4606425/149‑1 (MQ=255)

                    aaaaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGtt                                                                                                                            >  2:1145028/1‑149 (MQ=255)

                     aaaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTTGCGGTTCGCCTGGTGGTCGATCGTGATCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATTCCAGCACTACCACGCCATCATGTGAAGAGTTg                                                                                                                           >  1:4153668/1‑149 (MQ=255)

                     aaaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTg                                                                                                                           >  1:1763894/1‑149 (MQ=255)

                      aaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTgg                                                                                                                          >  2:3925223/1‑149 (MQ=255)

                      aaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTgg                                                                                                                          <  1:1935227/149‑1 (MQ=255)

                      aaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTgg                                                                                                                          >  2:3086851/1‑149 (MQ=255)

                        aGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTTAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCg                                                                                                                        >  1:749656/1‑149 (MQ=255)

                           tCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTa                                                                                                                     <  1:4274808/149‑1 (MQ=255)

                             gCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGGTCGCCGGGGGGTCGAGCGTGAGCGGGAAAGTAAAGCGGTCGTGCCGGAAGAGTTCTGGGACGTCGATGCCCCCACGACCCCGCCCACTGGTGAAGCGTTGGCGGTACa                                                                                                                   >  1:1243725/1‑149 (MQ=255)

                              cTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGGGAAATTAAAGCGTTCGTGCCGGGAGAGTTCTGGGCAGTCGATGCCAGCACAACCACGCCCTCTGGTGAAGCGGTGGCGTTACAg                                                                                                                  >  1:1386075/1‑149 (MQ=255)

                               tCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAgg                                                                                                                 <  2:4174054/149‑1 (MQ=255)

                                cGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGt                                                                                                                <  1:3251045/149‑1 (MQ=255)

                                 gTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTg                                                                                                               <  2:3225060/149‑1 (MQ=255)

                                  tGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGa                                                                                                              <  1:2958163/149‑1 (MQ=255)

                                       tGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACtcat                                                                                                         <  1:4122318/149‑1 (MQ=255)

                                       tGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACtcat                                                                                                         >  2:2164236/1‑149 (MQ=255)

                                         tCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACtcatca                                                                                                       <  2:3779602/149‑1 (MQ=255)

                                           tGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGa                                                                                                     >  1:4691536/1‑149 (MQ=255)

                                            gCCGGTCGTGTGCAGTCGGTGGCGGATCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGAGTTCGTGCCGGAAGAGTTCAGGGAAGTCGATTCCAGCACAACCACGCCATCTGGAGAAGCGTAGGAGTTACAGGTTAATCAACAGaa                                                                                                    >  2:1576900/1‑149 (MQ=255)

                                                gTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGAc                                                                                                >  1:4332350/1‑149 (MQ=255)

                                                 tCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACa                                                                                               <  2:4096097/149‑1 (MQ=255)

                                                    tgtgCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAAc                                                                                            >  2:4546427/1‑149 (MQ=255)

                                                      tgCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCg                                                                                          <  1:2354857/149‑1 (MQ=255)

                                                         aGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtc                                                                                       <  2:1309122/149‑1 (MQ=255)

                                                          gTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtcc                                                                                      <  1:4520798/149‑1 (MQ=255)

                                                           tCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtccg                                                                                     >  2:713012/1‑149 (MQ=255)

                                                              gTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtccgtcc                                                                                  >  2:2016212/1‑149 (MQ=255)

                                                                ggCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCgg                                                                                <  1:4433056/149‑1 (MQ=255)

                                                                  cGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTc                                                                              <  2:1283681/149‑1 (MQ=255)

                                                                  cGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTc                                                                              <  2:1055570/149‑1 (MQ=255)

                                                                  cGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTc                                                                              <  2:4089783/149‑1 (MQ=255)

                                                                  cGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTc                                                                              <  1:3381661/149‑1 (MQ=255)

                                                                       cGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaacaa                                                                         >  2:280333/1‑149 (MQ=255)

                                                                         ccTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAg                                                                       >  1:1011385/1‑149 (MQ=255)

                                                                          cTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGa                                                                      <  1:2484876/149‑1 (MQ=255)

                                                                            ggtggtCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAAc                                                                    <  2:1531629/149‑1 (MQ=255)

                                                                               ggtCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACaaa                                                                 >  2:55865/1‑149 (MQ=255)

                                                                                gtCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGACGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAAc                                                                >  2:1461201/1‑149 (MQ=255)

                                                                                    agcgtgagcgtgAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAg                                                            <  1:2027056/149‑1 (MQ=255)

                                                                                    agcgtgagcgtgAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAg                                                            >  1:4094405/1‑149 (MQ=255)

                                                                                     gcgtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAgg                                                           >  1:4334681/1‑149 (MQ=255)

                                                                                          agcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCg                                                      <  2:285736/149‑1 (MQ=255)

                                                                                           gcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCgg                                                     <  1:1583038/149‑1 (MQ=255)

                                                                                            cgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGt                                                    <  1:2463495/149‑1 (MQ=255)

                                                                                            cgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGt                                                    <  2:1335481/149‑1 (MQ=255)

                                                                                              tgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTaa                                                  <  1:3853655/149‑1 (MQ=255)

                                                                                              tgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTaa                                                  <  2:4228648/149‑1 (MQ=255)

                                                                                               taaaTTAAAGCGTTCGTGCCTGAAGAGTTCTGGGAAGTCGATGCCAGCACTAACACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAg                                                 <  1:429576/148‑1 (MQ=255)

                                                                                                aaaTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGt                                                >  1:3030102/1‑149 (MQ=255)

                                                                                                 aaTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTc                                               <  1:4144006/149‑1 (MQ=255)

                                                                                                 aaTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTc                                               >  2:2004100/1‑149 (MQ=255)

                                                                                                  aTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTct                                              >  2:3022193/1‑149 (MQ=255)

                                                                                                   ttAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctg                                             >  1:2745853/1‑149 (MQ=255)

                                                                                                    tAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctgc                                            <  1:2014625/149‑1 (MQ=255)

                                                                                                     aaaGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctgct                                           >  1:29079/1‑149 (MQ=255)

                                                                                                      aaGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctgctg                                          >  2:2320097/1‑149 (MQ=255)

                                                                                                          gTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGaaa                                      >  2:1691396/1‑149 (MQ=255)

                                                                                                           ttCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGaaaa                                     >  1:212000/1‑149 (MQ=255)

                                                                                                               tGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcg                                 <  2:2761867/149‑1 (MQ=255)

                                                                                                                gCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgc                                <  2:4087575/149‑1 (MQ=255)

                                                                                                                  cGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgcgc                              <  2:4661266/149‑1 (MQ=255)

                                                                                                                  cGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgcgc                              <  1:421301/149‑1 (MQ=255)

                                                                                                                     aaGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTAc                           <  1:4500202/149‑1 (MQ=255)

                                                                                                                     aaGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTAc                           <  1:863435/149‑1 (MQ=255)

                                                                                                                      agagTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACa                          <  2:3297930/149‑1 (MQ=255)

                                                                                                                       gagTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAg                         >  2:208382/1‑149 (MQ=255)

                                                                                                                            cTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGc                    <  2:1939589/149‑1 (MQ=255)

                                                                                                                            cTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGc                    <  1:751961/149‑1 (MQ=255)

                                                                                                                              gggAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTg                  <  2:3479430/149‑1 (MQ=255)

                                                                                                                                  aGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAAc              >  1:1808252/1‑149 (MQ=255)

                                                                                                                                  aGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAAc              >  1:1163839/1‑149 (MQ=255)

                                                                                                                                  aGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAAc              >  1:1164052/1‑149 (MQ=255)

                                                                                                                                   gTCGATGCCAGCACTACCACGCCATCTGGCGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACg             >  1:64392/1‑149 (MQ=255)

                                                                                                                                     cGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTg           <  1:2250096/149‑1 (MQ=255)

                                                                                                                                       aTGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGaa         >  1:1294393/1‑149 (MQ=255)

                                                                                                                                          ccAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGAc      >  1:2578853/1‑149 (MQ=255)

                                                                                                                                           cAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACa     <  2:1829264/149‑1 (MQ=255)

                                                                                                                                            aGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACaa    >  1:4672025/1‑149 (MQ=255)

                                                                                                                                              cACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAAc  <  2:2114427/149‑1 (MQ=255)

                                                                                                                                              cACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAAc  <  2:1813445/149‑1 (MQ=255)

                                                                                                                                              cACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAAc  <  1:972122/149‑1 (MQ=255)

                                                                                                                                              cACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAAc  <  2:1601245/149‑1 (MQ=255)

                                                                                                                                              cACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAAc  <  2:1485609/149‑1 (MQ=255)

TGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAAC  >  CP009273/1325837‑1326127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGA  >  CP009273/1325834‑1326130

ATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTA                                                                                                                                                     >  SRR19973071.904727/1‑150 (MQ=60)

  ATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACC                                                                                                                                                   >  SRR19973071.3541527/1‑150 (MQ=60)

  ATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACC                                                                                                                                                   >  SRR19973071.3541586/1‑150 (MQ=60)

   TGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCA                                                                                                                                                  <  SRR19973071.3538377/150‑1 (MQ=60)

    GGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCAC                                                                                                                                                 >  SRR19973071.2883547/1‑150 (MQ=60)

    GGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCAC                                                                                                                                                 <  SRR19973071.3467449/150‑1 (MQ=60)

    GGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCAC                                                                                                                                                 <  SRR19973071.3467477/150‑1 (MQ=60)

       TTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGGGAGCGTGAAATTAAATCGTTCGTGCCGGAAGAGTTCGGGGAAGTCGATGCCAGCACGACCACCCC                                                                                                                                              >  SRR19973071.1622465/1‑150 (MQ=60)

         CGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCAT                                                                                                                                            >  SRR19973071.2894561/1‑150 (MQ=60)

         CGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACAACCACGCCAA                                                                                                                                            >  SRR19973071.2894933/1‑150 (MQ=60)

           CCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATTCCAGCACTACCACcccaaca                                                                                                                                          >  SRR19973071.4439470/1‑143 (MQ=60)

           CCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGGGAAATTAAAGCGTTAGTGCCGGAAGAGTTCTGGGAAGTCGATGCCCGCACAACCACGCCATCT                                                                                                                                          >  SRR19973071.4439415/1‑150 (MQ=60)

                  TATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAG                                                                                                                                   <  SRR19973071.4588040/150‑1 (MQ=60)

                    TGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCG                                                                                                                                 <  SRR19973071.1197375/150‑1 (MQ=60)

                     GGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGT                                                                                                                                >  SRR19973071.1357273/1‑150 (MQ=60)

                      GAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTT                                                                                                                               >  SRR19973071.2748452/1‑150 (MQ=60)

                       AAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTTGCGGTTCGCCTGGTGGTCGATCGTGATCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATTCCAGCACTACCACGCCATCATGTGAAGAGTTG                                                                                                                              >  SRR19973071.4168457/1‑150 (MQ=60)

                       AAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTG                                                                                                                              >  SRR19973071.1770480/1‑150 (MQ=60)

                        AAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAATCGTTCGTGCCGGAAGAGGTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGG                                                                                                                             >  SRR19973071.2834001/1‑150 (MQ=60)

                        AAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGG                                                                                                                             >  SRR19973071.1518397/1‑150 (MQ=60)

                         AAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGC                                                                                                                            <  SRR19973071.777520/150‑1 (MQ=60)

                         AAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGC                                                                                                                            <  SRR19973071.777717/150‑1 (MQ=60)

                         AAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGC                                                                                                                            <  SRR19973071.1942281/150‑1 (MQ=60)

                         AAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGC                                                                                                                            <  SRR19973071.1743650/150‑1 (MQ=60)

                          AAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTTAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCG                                                                                                                           >  SRR19973071.752605/1‑150 (MQ=60)

                          AAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTTAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCG                                                                                                                           >  SRR19973071.752228/1‑150 (MQ=60)

                              TCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTAC                                                                                                                       <  SRR19973071.4289953/150‑1 (MQ=60)

                               CGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGGTCGCCGGGGGGTCGAGCGTGAGCGGGAAAGTAAAGCGGTCGTGCCGGAAGAGTTCTGGGACGTCGATGCCCCCACGACCCCGCCCACTGGTGAAGCGTTGGCGGTACA                                                                                                                      >  SRR19973071.1248159/1‑150 (MQ=60)

                                GCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGGGAAATTAAAGCGTTCGTGCCGGGAGAGTTCTGGGCAGTCGATGCCAGCACAACCACGCCCTCTGGTGAAGCGGTGGCGTTACAG                                                                                                                     >  SRR19973071.1391189/1‑150 (MQ=60)

                                   CGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTG                                                                                                                  <  SRR19973071.3262517/150‑1 (MQ=60)

                                     TGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGAC                                                                                                                <  SRR19973071.2968757/150‑1 (MQ=60)

                                          TGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATC                                                                                                           <  SRR19973071.4136993/150‑1 (MQ=60)

                                          TGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATC                                                                                                           <  SRR19973071.4136922/150‑1 (MQ=60)

                                            TCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAG                                                                                                         <  SRR19973071.4658619/150‑1 (MQ=60)

                                             CTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGA                                                                                                        >  SRR19973071.4708202/1‑150 (MQ=60)

                                                  GGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGAC                                                                                                   >  SRR19973071.4347635/1‑150 (MQ=60)

                                                      GTGTGCAGTCGGTGGCGGTTCGCCTTGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTTAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAAC                                                                                               >  SRR19973071.314442/1‑150 (MQ=60)

                                                       TGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACC                                                                                              >  SRR19973071.3377575/1‑150 (MQ=60)

                                                       TGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACC                                                                                              >  SRR19973071.4271366/1‑150 (MQ=60)

                                                         TGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGT                                                                                            <  SRR19973071.2363390/150‑1 (MQ=60)

                                                           CAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTC                                                                                          >  SRR19973071.639426/1‑150 (MQ=60)

                                                            AGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCC                                                                                         <  SRR19973071.1232970/150‑1 (MQ=60)

                                                            AGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCC                                                                                         <  SRR19973071.4301175/150‑1 (MQ=60)

                                                            AGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCC                                                                                         <  SRR19973071.667658/150‑1 (MQ=60)

                                                            AGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCC                                                                                         <  SRR19973071.668706/150‑1 (MQ=60)

                                                             GTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCG                                                                                        <  SRR19973071.4536893/150‑1 (MQ=60)

                                                                GGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCC                                                                                     <  SRR19973071.2295847/150‑1 (MQ=60)

                                                                GGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCC                                                                                     <  SRR19973071.248766/150‑1 (MQ=60)

                                                                   GGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGT                                                                                  <  SRR19973071.4203430/150‑1 (MQ=60)

                                                                   GGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGT                                                                                  <  SRR19973071.4448702/150‑1 (MQ=60)

                                                                   GGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGT                                                                                  <  SRR19973071.1093450/150‑1 (MQ=60)

                                                                     CGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCA                                                                                >  SRR19973071.2782323/1‑150 (MQ=60)

                                                                     CGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCA                                                                                <  SRR19973071.1879396/150‑1 (MQ=60)

                                                                     CGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCA                                                                                <  SRR19973071.2405942/150‑1 (MQ=60)

                                                                     CGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCA                                                                                <  SRR19973071.3393656/150‑1 (MQ=60)

                                                                     CGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCA                                                                                <  SRR19973071.3155/150‑1 (MQ=60)

                                                                      GGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAA                                                                               >  SRR19973071.4349787/1‑150 (MQ=60)

                                                                       GTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAAC                                                                              <  SRR19973071.2259873/150‑1 (MQ=60)

                                                                           GCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAG                                                                          >  SRR19973071.1015057/1‑150 (MQ=60)

                                                                           GCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAG                                                                          >  SRR19973071.3411808/1‑150 (MQ=60)

                                                                            CCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGA                                                                         <  SRR19973071.1705747/150‑1 (MQ=60)

                                                                             CTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAA                                                                        <  SRR19973071.2493918/150‑1 (MQ=60)

                                                                                GTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAA                                                                     >  SRR19973071.3027986/1‑150 (MQ=60)

                                                                                   GTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACT                                                                  <  SRR19973071.4401243/150‑1 (MQ=60)

                                                                                      GAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAG                                                               >  SRR19973071.4108953/1‑150 (MQ=60)

                                                                                      GAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAG                                                               >  SRR19973071.4108952/1‑150 (MQ=60)

                                                                                      GAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGGCCGGTCAACAAAGAACAAACTCAG                                                               >  SRR19973071.4488782/1‑150 (MQ=60)

                                                                                       AGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGG                                                              <  SRR19973071.2034437/150‑1 (MQ=60)

                                                                                       AGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGG                                                              <  SRR19973071.2034249/150‑1 (MQ=60)

                                                                                       AGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGG                                                              <  SRR19973071.97831/150‑1 (MQ=60)

                                                                                       AGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGG                                                              >  SRR19973071.4349971/1‑150 (MQ=60)

                                                                                       AGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGG                                                              >  SRR19973071.2905932/1‑150 (MQ=60)

                                                                                              GCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGT                                                       <  SRR19973071.1588645/150‑1 (MQ=60)

                                                                                              GCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGT                                                       <  SRR19973071.1588679/150‑1 (MQ=60)

                                                                                               CGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTA                                                      <  SRR19973071.2472451/150‑1 (MQ=60)

                                                                                                 TGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAG                                                    <  SRR19973071.3867305/150‑1 (MQ=60)

                                                                                                  TAAATTAAAGCGTTCGTGCCTGAAGAGTTCTGGGAAGTCGATGCCAGCACTAACACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGT                                                   <  SRR19973071.431048/150‑1 (MQ=60)

                                                                                                  GAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGT                                                   <  SRR19973071.430833/150‑1 (MQ=60)

                                                                                                  GAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGT                                                   >  SRR19973071.3040886/1‑150 (MQ=60)

                                                                                                    AATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCT                                                 >  SRR19973071.2514944/1‑150 (MQ=60)

                                                                                                    AATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCT                                                 <  SRR19973071.4158728/150‑1 (MQ=60)

                                                                                                    AATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCT                                                 <  SRR19973071.4158761/150‑1 (MQ=60)

                                                                                                    AATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCT                                                 <  SRR19973071.4159097/150‑1 (MQ=60)

                                                                                                     ATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTG                                                >  SRR19973071.2755576/1‑150 (MQ=60)

                                                                                                       TAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCT                                              >  SRR19973071.29268/1‑150 (MQ=60)

                                                                                                       TAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCT                                              >  SRR19973071.29420/1‑150 (MQ=60)

                                                                                                       TAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCT                                              <  SRR19973071.3627003/150‑1 (MQ=60)

                                                                                                       TAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCT                                              <  SRR19973071.2021929/150‑1 (MQ=60)

                                                                                                       TAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCT                                              <  SRR19973071.2021875/150‑1 (MQ=60)

                                                                                                          AGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGA                                           >  SRR19973071.2912598/1‑150 (MQ=60)

                                                                                                             GTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAA                                        >  SRR19973071.212850/1‑150 (MQ=60)

                                                                                                                     CGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCT                                <  SRR19973071.421991/150‑1 (MQ=60)

                                                                                                                     CGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCT                                <  SRR19973071.2519056/150‑1 (MQ=60)

                                                                                                                     CGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCT                                <  SRR19973071.422716/150‑1 (MQ=60)

                                                                                                                      GGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTA                               <  SRR19973071.4525233/150‑1 (MQ=60)

                                                                                                                       GAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTAC                              >  SRR19973071.1259751/1‑150 (MQ=60)

                                                                                                                        AAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACA                             <  SRR19973071.2109999/150‑1 (MQ=60)

                                                                                                                        AAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACA                             >  SRR19973071.949206/1‑150 (MQ=60)

                                                                                                                        AAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACA                             >  SRR19973071.1800549/1‑150 (MQ=60)

                                                                                                                        AAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACA                             <  SRR19973071.4516197/150‑1 (MQ=60)

                                                                                                                        AAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACA                             <  SRR19973071.866520/150‑1 (MQ=60)

                                                                                                                         AGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAG                            <  SRR19973071.449417/150‑1 (MQ=60)

                                                                                                                           AGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCG                          >  SRR19973071.2097866/1‑150 (MQ=60)

                                                                                                                           AGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCG                          <  SRR19973071.4317472/150‑1 (MQ=60)

                                                                                                                               CTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCT                      <  SRR19973071.3537429/150‑1 (MQ=60)

                                                                                                                               CTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCT                      <  SRR19973071.754537/150‑1 (MQ=60)

                                                                                                                                TGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTG                     <  SRR19973071.4377705/150‑1 (MQ=60)

                                                                                                                                TGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTG                     >  SRR19973071.1382157/1‑150 (MQ=60)

                                                                                                                                    AAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAAC                 >  SRR19973071.1814992/1‑150 (MQ=60)

                                                                                                                                    AAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAAC                 >  SRR19973071.1167862/1‑150 (MQ=60)

                                                                                                                                    AAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAAC                 >  SRR19973071.1167907/1‑150 (MQ=60)

                                                                                                                                    AAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAAC                 >  SRR19973071.1168075/1‑150 (MQ=60)

                                                                                                                                     AGTCGATGCCAGCACTACCACGCCATCTGGCGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACG                >  SRR19973071.64752/1‑150 (MQ=60)

                                                                                                                                        CGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGA             <  SRR19973071.2258392/150‑1 (MQ=60)

                                                                                                                                        CGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGA             >  SRR19973071.2190759/1‑150 (MQ=60)

                                                                                                                                         GATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAA            >  SRR19973071.1299112/1‑150 (MQ=60)

                                                                                                                                          ATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAG           >  SRR19973071.3675681/1‑150 (MQ=60)

                                                                                                                                           TGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGA          <  SRR19973071.1924675/150‑1 (MQ=60)

                                                                                                                                            GCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGAC         >  SRR19973071.2588201/1‑150 (MQ=60)

                                                                                                                                            GCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGAC         >  SRR19973071.3907959/1‑150 (MQ=60)

                                                                                                                                              CAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAA       >  SRR19973071.4688668/1‑150 (MQ=60)

                                                                                                                                              CAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAA       >  SRR19973071.4688659/1‑150 (MQ=60)

                                                                                                                                                 CACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACC    <  SRR19973071.975652/150‑1 (MQ=60)

                                                                                                                                                   CTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGA  >  SRR19973071.1296355/1‑150 (MQ=60)

                                                                                                                                                   CTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGA  >  SRR19973071.1296602/1‑150 (MQ=60)

ATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGA  >  CP009273/1325834‑1326130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`