Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,325,982 G→T 100% T226T (ACG→ACT)  topA → DNA topoisomerase I, omega subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,325,9820GT97.5% 467.4 / 3.7 160T226T (ACG→ACT) topADNA topoisomerase I, omega subunit
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (77/77);  total (82/78)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 160 total aligned reads displayed.

ATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGA  >  CP009273/1325836‑1326122
                                                                                                                                                  |                                                                                                                                            
atGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGa                                                                                                                                             <  1:2683215/148‑1 (MQ=255)
 tGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTAc                                                                                                                                            >  1:84861/1‑148 (MQ=255)
 gggTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTAc                                                                                                                                            <  2:2942862/147‑1 (MQ=255)
  ggTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTAcc                                                                                                                                           >  1:2373703/1‑148 (MQ=255)
  ggTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTAcc                                                                                                                                           >  2:2775089/1‑148 (MQ=255)
  ggTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTAcc                                                                                                                                           <  1:1580049/148‑1 (MQ=255)
        gccgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCa                                                                                                                                     <  1:841602/148‑1 (MQ=255)
         ccgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCAt                                                                                                                                    >  2:850203/1‑148 (MQ=255)
         ccgcTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCAt                                                                                                                                    <  1:1777634/148‑1 (MQ=255)
            ctgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTg                                                                                                                                 <  1:3029207/148‑1 (MQ=255)
            ctgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTg                                                                                                                                 >  2:2212718/1‑148 (MQ=255)
            ctgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTg                                                                                                                                 <  1:290588/148‑1 (MQ=255)
             tgctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAATCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTgg                                                                                                                                >  1:3061411/1‑148 (MQ=255)
              gctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGt                                                                                                                               >  2:2055195/1‑148 (MQ=255)
              gctATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGt                                                                                                                               >  2:1469250/1‑148 (MQ=255)
                tATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGa                                                                                                                             <  1:2527351/148‑1 (MQ=255)
                tATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGa                                                                                                                             <  1:1113324/148‑1 (MQ=255)
                tATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGa                                                                                                                             <  1:744987/148‑1 (MQ=255)
                     aaaaaGATTGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGt                                                                                                                        >  1:508313/1‑148 (MQ=255)
                     aaaaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGt                                                                                                                        <  2:198507/148‑1 (MQ=255)
                      aaaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCGGGTGGTCGAGCGTGAGCGGGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGtt                                                                                                                       >  1:1032303/1‑148 (MQ=255)
                       aaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTg                                                                                                                      >  1:3243069/1‑148 (MQ=255)
                       aaaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTg                                                                                                                      <  2:589081/148‑1 (MQ=255)
                        aaGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTgg                                                                                                                     >  2:2083325/1‑148 (MQ=255)
                          gATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCg                                                                                                                   <  1:724217/148‑1 (MQ=255)
                           aTCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGt                                                                                                                  <  1:1482065/148‑1 (MQ=255)
                            tCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGtt                                                                                                                 >  1:2640163/1‑148 (MQ=255)
                                 cGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAgg                                                                                                            >  1:2047940/1‑148 (MQ=255)
                                 cGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAgg                                                                                                            >  1:2396477/1‑148 (MQ=255)
                                    ggCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGa                                                                                                         <  1:121506/148‑1 (MQ=255)
                                     gCCTGTCTGCCGGTCGTGTGCAGTCGGTTGCCGTTCGCCGGGTGGTCGAGCGTGAGCGTGAAATTAAATCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACCCCATATGGTGAAGCGTTGGCGTTACACGTGAc                                                                                                        >  1:2722611/1‑148 (MQ=255)
                                     gCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGCAGTCGATGCCAGCACTACCACGCCAACAGGTGAAGCGTTGGCGTTACACGTGAc                                                                                                        >  1:946343/1‑148 (MQ=255)
                                     gCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGAc                                                                                                        >  2:113054/1‑148 (MQ=255)
                                         gTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACtcat                                                                                                    >  1:1914394/1‑148 (MQ=255)
                                           cTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACtcatca                                                                                                  >  2:2336954/1‑148 (MQ=255)
                                              ccGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGaa                                                                                               >  2:395017/1‑148 (MQ=255)
                                                ggTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACg                                                                                             <  2:857439/148‑1 (MQ=255)
                                                 gTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGa                                                                                            <  2:1599553/148‑1 (MQ=255)
                                                   cGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACa                                                                                          >  1:393330/1‑148 (MQ=255)
                                                     tgtgCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACaaa                                                                                        <  1:3424625/148‑1 (MQ=255)
                                                     tgtgCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACaaa                                                                                        <  1:539285/148‑1 (MQ=255)
                                                     tgtgCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACaaa                                                                                        <  1:539311/148‑1 (MQ=255)
                                                        gCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCg                                                                                     <  1:3114324/148‑1 (MQ=255)
                                                        gCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCg                                                                                     <  1:1344313/148‑1 (MQ=255)
                                                            tCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtcc                                                                                 >  2:1121648/1‑148 (MQ=255)
                                                              ggTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTtccgt                                                                               <  2:2066879/148‑1 (MQ=255)
                                                                      ttCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaa                                                                       <  2:105944/148‑1 (MQ=255)
                                                                      ttCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaa                                                                       >  2:2552605/1‑148 (MQ=255)
                                                                      ttCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaa                                                                       <  1:2932195/148‑1 (MQ=255)
                                                                       tCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaac                                                                      >  2:762860/1‑148 (MQ=255)
                                                                         gCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaacaa                                                                    <  2:2872782/148‑1 (MQ=255)
                                                                         gCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTcaacaa                                                                    <  2:632545/148‑1 (MQ=255)
                                                                          ccTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACaaa                                                                   >  1:2083084/1‑148 (MQ=255)
                                                                            tggtggTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGa                                                                 <  2:1007763/148‑1 (MQ=255)
                                                                              gtggtCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAAc                                                               <  1:1971693/148‑1 (MQ=255)
                                                                                  tCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAAc                                                           >  1:2942494/1‑148 (MQ=255)
                                                                                       cgtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAgg                                                      <  2:2569291/148‑1 (MQ=255)
                                                                                        gtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGc                                                     <  1:3342428/148‑1 (MQ=255)
                                                                                        gtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGc                                                     <  1:3342432/148‑1 (MQ=255)
                                                                                        gtgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGc                                                     <  2:2857482/148‑1 (MQ=255)
                                                                                         tgagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCt                                                    >  2:526718/1‑148 (MQ=255)
                                                                                          gagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTg                                                   <  1:978703/148‑1 (MQ=255)
                                                                                          gagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTg                                                   <  1:2290630/148‑1 (MQ=255)
                                                                                          gagcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTg                                                   >  2:3370293/1‑148 (MQ=255)
                                                                                            gcgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCg                                                 <  2:3243069/148‑1 (MQ=255)
                                                                                             cgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCgg                                                <  2:2552508/148‑1 (MQ=255)
                                                                                             cgtgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCgg                                                <  2:2552450/148‑1 (MQ=255)
                                                                                               tgaAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTa                                              >  1:3470521/1‑148 (MQ=255)
                                                                                                   aTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTc                                          >  1:2813081/1‑148 (MQ=255)
                                                                                                   aTTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTc                                          >  2:1462470/1‑148 (MQ=255)
                                                                                                     tAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctg                                        <  2:498007/148‑1 (MQ=255)
                                                                                                     tAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctg                                        >  1:593697/1‑148 (MQ=255)
                                                                                                      aaaGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGGTGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctgc                                       >  2:2145526/1‑148 (MQ=255)
                                                                                                      aaaGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctgc                                       >  2:351673/1‑148 (MQ=255)
                                                                                                        aGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctgctg                                     >  1:582192/1‑148 (MQ=255)
                                                                                                        aGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTctgctg                                     >  1:582359/1‑148 (MQ=255)
                                                                                                         gCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTgg                                    >  1:3242045/1‑148 (MQ=255)
                                                                                                                    ggAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgcgc                         >  1:2085066/1‑148 (MQ=255)
                                                                                                                    ggAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgcgc                         >  1:519867/1‑148 (MQ=255)
                                                                                                                    ggAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAgcgcgc                         >  2:2975322/1‑148 (MQ=255)
                                                                                                                     gAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCt                        >  2:899096/1‑148 (MQ=255)
                                                                                                                     gAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCt                        <  1:2320580/148‑1 (MQ=255)
                                                                                                                     gAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCt                        >  2:1262353/1‑148 (MQ=255)
                                                                                                                      aaGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTa                       >  1:1289134/1‑148 (MQ=255)
                                                                                                                      aaGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTa                       <  2:1032303/148‑1 (MQ=255)
                                                                                                                           ttCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCg                  >  1:2885569/1‑148 (MQ=255)
                                                                                                                             cTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTg                <  1:28296/148‑1 (MQ=255)
                                                                                                                             cTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTg                <  2:1936946/148‑1 (MQ=255)
                                                                                                                             cTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTg                <  2:3279247/148‑1 (MQ=255)
                                                                                                                             cTGCGACGTCGCTGCCAGCCCTCCCACGCCATCTGGTGCAGCGTTGGCGTTCCCGGTGACTCATCAGAACGACAAACCGTGCCGTCCGGTCAACAACGAACAAACTCAGGCTGCGGTAAGTCTGCGGGAAAAAGCGCGCTACAGCGTg                <  2:1937043/148‑1 (MQ=255)
                                                                                                                                ggAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTg             >  1:465395/1‑148 (MQ=255)
                                                                                                                                  aaGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGa           <  1:3080149/148‑1 (MQ=255)
                                                                                                                                     tCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCGACAGCGTGCTGGAACg        <  1:113054/148‑1 (MQ=255)
                                                                                                                                      cGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGt       >  1:1974911/1‑148 (MQ=255)
                                                                                                                                      cGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGt       >  2:172145/1‑148 (MQ=255)
                                                                                                                                      cGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGt       >  2:172146/1‑148 (MQ=255)
                                                                                                                                      cGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGt       >  1:1974869/1‑148 (MQ=255)
                                                                                                                                           ccAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGa  >  2:2680140/1‑148 (MQ=255)
                                                                                                                                           ccAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAAATCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGa  <  1:1004078/148‑1 (MQ=255)
                                                                                                                                           ccAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTACGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGa  >  2:1829902/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
ATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGA  >  CP009273/1325836‑1326122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGAC  >  CP009273/1325833‑1326131
                                                                                                                                                     |                                                                                                                                                     
TATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACT                                                                                                                                                       >  SRR19973067.1315333/1‑150 (MQ=60)
TATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACT                                                                                                                                                       >  SRR19973067.1315299/1‑150 (MQ=60)
  TATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTAC                                                                                                                                                     >  SRR19973067.85787/1‑150 (MQ=60)
   ATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACC                                                                                                                                                    >  SRR19973067.2389138/1‑150 (MQ=60)
   ATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACC                                                                                                                                                    <  SRR19973067.2700407/150‑1 (MQ=60)
     GGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCAC                                                                                                                                                  <  SRR19973067.1590871/150‑1 (MQ=60)
      GTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCGGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACG                                                                                                                                                 >  SRR19973067.760610/1‑150 (MQ=60)
        TTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCC                                                                                                                                               <  SRR19973067.3118837/150‑1 (MQ=60)
           GCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATC                                                                                                                                            <  SRR19973067.847314/150‑1 (MQ=60)
            CCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCT                                                                                                                                           <  SRR19973067.1789669/150‑1 (MQ=60)
              GCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAATCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGG                                                                                                                                         >  SRR19973067.3080900/1‑150 (MQ=60)
               CTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGT                                                                                                                                        <  SRR19973067.3048490/150‑1 (MQ=60)
               CTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGT                                                                                                                                        <  SRR19973067.292714/150‑1 (MQ=60)
               CTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGT                                                                                                                                        <  SRR19973067.292465/150‑1 (MQ=60)
                   TATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAG                                                                                                                                    <  SRR19973067.1120877/150‑1 (MQ=60)
                   TATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAG                                                                                                                                    <  SRR19973067.1120604/150‑1 (MQ=60)
                   TATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAG                                                                                                                                    <  SRR19973067.2241116/150‑1 (MQ=60)
                   TATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAG                                                                                                                                    <  SRR19973067.750155/150‑1 (MQ=60)
                   TATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAG                                                                                                                                    <  SRR19973067.2543919/150‑1 (MQ=60)
                     TGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCG                                                                                                                                  >  SRR19973067.3452852/1‑150 (MQ=60)
                      GGAAAAAGATTGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGT                                                                                                                                 >  SRR19973067.511780/1‑150 (MQ=60)
                       GAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCGGGTGGTCGAGCGTGAGCGGGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCAACTGGTGAAGCGTT                                                                                                                                >  SRR19973067.1039412/1‑150 (MQ=60)
                        AAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTG                                                                                                                               >  SRR19973067.3263389/1‑150 (MQ=60)
                          AAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGC                                                                                                                             <  SRR19973067.2036695/150‑1 (MQ=60)
                            AGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACAACCACGCCCACTGGTGAAGCGTTGGCGT                                                                                                                           >  SRR19973067.3324835/1‑150 (MQ=60)
                             GATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTT                                                                                                                          <  SRR19973067.729251/150‑1 (MQ=60)
                             GATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTT                                                                                                                          >  SRR19973067.2657226/1‑150 (MQ=60)
                              ATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTA                                                                                                                         <  SRR19973067.1491898/150‑1 (MQ=60)
                                  CTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTGGCCGGGTGGTCGAGCGTGAGCGTGAAAATAAATCGTTCGTGCCGGAAGAGTTATGGGAAGTCGATTCCAGCACTACCACCCCATCTGGGGAAGCGTTTGCGTTACAGG                                                                                                                     >  SRR19973067.615639/1‑150 (MQ=60)
                                  CTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGG                                                                                                                     >  SRR19973067.2061619/1‑150 (MQ=60)
                                  CTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGG                                                                                                                     >  SRR19973067.2412090/1‑150 (MQ=60)
                                      TGGCCTGTCTGCCGGTCGTGTGCAGTCGGTTGCCGTTCGCCGGGTGGTCGAGCGTGAGCGTGAAATTAAATCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACCCCATATGGTGAAGCGTTGGCGTTACACGTGAC                                                                                                                 >  SRR19973067.2740173/1‑150 (MQ=60)
                                      TGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGCAGTCGATGCCAGCACTACCACGCCAACAGGTGAAGCGTTGGCGTTACACGTGAC                                                                                                                 >  SRR19973067.952808/1‑150 (MQ=60)
                                       GGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACT                                                                                                                <  SRR19973067.122650/150‑1 (MQ=60)
                                        GCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTC                                                                                                               >  SRR19973067.3147288/1‑150 (MQ=60)
                                          CTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCAT                                                                                                             >  SRR19973067.1927460/1‑150 (MQ=60)
                                          CTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCAT                                                                                                             >  SRR19973067.1927163/1‑150 (MQ=60)
                                                    GTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACA                                                                                                   >  SRR19973067.396079/1‑150 (MQ=60)
                                                        TGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACC                                                                                               <  SRR19973067.542878/150‑1 (MQ=60)
                                                        TGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACC                                                                                               <  SRR19973067.542905/150‑1 (MQ=60)
                                                        TGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACC                                                                                               <  SRR19973067.3446420/150‑1 (MQ=60)
                                                        TGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACC                                                                                               <  SRR19973067.3446315/150‑1 (MQ=60)
                                                        TGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAAATAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACAACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACC                                                                                               >  SRR19973067.122038/1‑150 (MQ=60)
                                                           GCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTT                                                                                            <  SRR19973067.3134122/150‑1 (MQ=60)
                                                           GCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTT                                                                                            <  SRR19973067.1353513/150‑1 (MQ=60)
                                                                  GTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCG                                                                                     <  SRR19973067.2656445/150‑1 (MQ=60)
                                                                        GTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAAC                                                                               >  SRR19973067.3071637/1‑150 (MQ=60)
                                                                        GTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAAC                                                                               >  SRR19973067.1290197/1‑150 (MQ=60)
                                                                         TTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACA                                                                              <  SRR19973067.2950813/150‑1 (MQ=60)
                                                                         TTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACA                                                                              <  SRR19973067.2950893/150‑1 (MQ=60)
                                                                           CGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAA                                                                            >  SRR19973067.2096883/1‑150 (MQ=60)
                                                                           CGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAA                                                                            >  SRR19973067.2096867/1‑150 (MQ=60)
                                                                                 GTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAA                                                                      <  SRR19973067.1985062/150‑1 (MQ=60)
                                                                                   GGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAAC                                                                    >  SRR19973067.417749/1‑150 (MQ=60)
                                                                                   GGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAAC                                                                    >  SRR19973067.2961141/1‑150 (MQ=60)
                                                                                     TCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTC                                                                  <  SRR19973067.855952/150‑1 (MQ=60)
                                                                                      CGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCA                                                                 >  SRR19973067.97409/1‑150 (MQ=60)
                                                                                          CGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCT                                                             >  SRR19973067.516595/1‑150 (MQ=60)
                                                                                           GTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTG                                                            >  SRR19973067.137477/1‑150 (MQ=60)
                                                                                           GTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTG                                                            <  SRR19973067.3363608/150‑1 (MQ=60)
                                                                                           GTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTG                                                            <  SRR19973067.3363603/150‑1 (MQ=60)
                                                                                             GAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCG                                                          <  SRR19973067.985419/150‑1 (MQ=60)
                                                                                             GAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCG                                                          <  SRR19973067.2305765/150‑1 (MQ=60)
                                                                                                CGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTA                                                       >  SRR19973067.3492568/1‑150 (MQ=60)
                                                                                                  TGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAG                                                     <  SRR19973067.2393079/150‑1 (MQ=60)
                                                                                                    AAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTC                                                   >  SRR19973067.2831205/1‑150 (MQ=60)
                                                                                                    AAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTC                                                   >  SRR19973067.2831199/1‑150 (MQ=60)
                                                                                                     AATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCT                                                  <  SRR19973067.1752947/150‑1 (MQ=60)
                                                                                                     AATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCT                                                  >  SRR19973067.53408/1‑150 (MQ=60)
                                                                                                      ATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTG                                                 >  SRR19973067.597473/1‑150 (MQ=60)
                                                                                                      ATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTG                                                 >  SRR19973067.597472/1‑150 (MQ=60)
                                                                                                         AAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTG                                              >  SRR19973067.586089/1‑150 (MQ=60)
                                                                                                         AAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTG                                              >  SRR19973067.585921/1‑150 (MQ=60)
                                                                                                          AAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGG                                             >  SRR19973067.3262363/1‑150 (MQ=60)
                                                                                                                   TGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGC                                    <  SRR19973067.1987855/150‑1 (MQ=60)
                                                                                                                     CCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGC                                  >  SRR19973067.523382/1‑150 (MQ=60)
                                                                                                                     CCGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGC                                  >  SRR19973067.2098870/1‑150 (MQ=60)
                                                                                                                      CGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCT                                 <  SRR19973067.2388707/150‑1 (MQ=60)
                                                                                                                      CGGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCT                                 <  SRR19973067.2710496/150‑1 (MQ=60)
                                                                                                                       GGAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTA                                >  SRR19973067.1297934/1‑150 (MQ=60)
                                                                                                                        GAAGAGTTCTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTAC                               <  SRR19973067.2335839/150‑1 (MQ=60)
                                                                                                                            AGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCG                           >  SRR19973067.2904014/1‑150 (MQ=60)
                                                                                                                                CTGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCT                       <  SRR19973067.28737/150‑1 (MQ=60)
                                                                                                                                 TGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTG                      >  SRR19973067.567263/1‑150 (MQ=60)
                                                                                                                                 TGGGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTG                      >  SRR19973067.468611/1‑150 (MQ=60)
                                                                                                                                   GGAAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTGCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGA                    >  SRR19973067.2878327/1‑150 (MQ=60)
                                                                                                                                     AAGTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAAC                  <  SRR19973067.3099751/150‑1 (MQ=60)
                                                                                                                                       GTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGT                >  SRR19973067.1988252/1‑150 (MQ=60)
                                                                                                                                       GTCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGT                >  SRR19973067.1988294/1‑150 (MQ=60)
                                                                                                                                        TCGATGCCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCGACAGCGTGCTGGAACGTG               <  SRR19973067.114150/150‑1 (MQ=60)
                                                                                                                                              CCAGCACTACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAAATCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACA         <  SRR19973067.1010975/150‑1 (MQ=60)
                                                                                                                                                     TACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGAC  >  SRR19973067.2570858/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACCACGCCATCTGGTGAAGCGTTGGCGTTACAGGTGACTCATCAGAACGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGAC  >  CP009273/1325833‑1326131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑