Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 248,486 C→A 100% intergenic (+46/‑6) dinB → / → yafN DNA polymerase IV/antitoxin of the YafO‑YafN toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273248,4860CA98.7% 712.4 / 0.7 224intergenic (+46/‑6)dinB/yafNDNA polymerase IV/antitoxin of the YafO‑YafN toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (116/105);  total (118/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 226 total aligned reads displayed.

CTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCG  >  CP009273/248341‑248630
                                                                                                                                                 |                                                                                                                                                
   ggATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGt                                                                                                                                             >  2:1019465/1‑148 (MQ=255)
   ggATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGt                                                                                                                                             <  2:281960/148‑1 (MQ=255)
   ggATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGt                                                                                                                                             >  2:788333/1‑148 (MQ=255)
   ggATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCAGTATTATGAGGGTATAACTGt                                                                                                                                             >  2:1767805/1‑148 (MQ=255)
       gAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTCTAACTGTATGc                                                                                                                                         >  1:1704777/1‑148 (MQ=255)
       gAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGc                                                                                                                                         >  1:1022059/1‑148 (MQ=255)
       gAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGc                                                                                                                                         <  1:1153814/148‑1 (MQ=255)
         aCGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCAt                                                                                                                                       <  2:2519350/148‑1 (MQ=255)
             cgcgGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGaa                                                                                                                                   <  1:1397226/148‑1 (MQ=255)
              gcggcgGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAAt                                                                                                                                  <  2:678002/148‑1 (MQ=255)
               cggcggGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAAtt                                                                                                                                 <  1:1259090/148‑1 (MQ=255)
                ggcgggcgCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATtc                                                                                                                                <  1:2985529/148‑1 (MQ=255)
                   gggcgCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCg                                                                                                                             <  2:3354523/148‑1 (MQ=255)
                    ggcgCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGc                                                                                                                            >  1:2820656/1‑148 (MQ=255)
                      cgcgGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTg                                                                                                                          >  1:3396667/1‑148 (MQ=255)
                       gcgGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGa                                                                                                                         <  2:2574212/148‑1 (MQ=255)
                        cgGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGaa                                                                                                                        <  2:2347155/148‑1 (MQ=255)
                        cgGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGaa                                                                                                                        <  1:1223678/148‑1 (MQ=255)
                         ggTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGaaa                                                                                                                       <  1:859339/148‑1 (MQ=255)
                           tgtgCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGaaaaa                                                                                                                     <  1:1584622/148‑1 (MQ=255)
                             tgCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATc                                                                                                                   <  1:155876/148‑1 (MQ=255)
                              gCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCg                                                                                                                  >  2:3033338/1‑148 (MQ=255)
                               cGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCgg                                                                                                                 <  2:1969609/148‑1 (MQ=255)
                                  cTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCa                                                                                                              <  1:2668934/148‑1 (MQ=255)
                                     gtggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAata                                                                                                           >  2:2438963/1‑148 (MQ=255)
                                     gtggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAata                                                                                                           >  2:1708452/1‑148 (MQ=255)
                                      tggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAatat                                                                                                          <  2:1261347/148‑1 (MQ=255)
                                       ggggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATc                                                                                                         >  2:2161624/1‑148 (MQ=255)
                                         ggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCAc                                                                                                       >  2:2068924/1‑148 (MQ=255)
                                               aTGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGtt                                                                                                 >  2:1487623/1‑148 (MQ=255)
                                                 gtgACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTAc                                                                                               >  2:594426/1‑148 (MQ=255)
                                                 gtgACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTAc                                                                                               <  1:3327630/148‑1 (MQ=255)
                                                  tgACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACg                                                                                              >  1:2146365/1‑148 (MQ=255)
                                                           ttGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAAccc                                                                                     <  2:1088076/148‑1 (MQ=255)
                                                                   cAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAAt                                                                             <  1:133556/148‑1 (MQ=255)
                                                                      aTGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACt                                                                          >  2:3110572/1‑148 (MQ=255)
                                                                        ggAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACttt                                                                        >  1:642923/1‑148 (MQ=255)
                                                                         gAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTa                                                                       >  2:2049259/1‑148 (MQ=255)
                                                                         gAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTa                                                                       <  1:2704405/148‑1 (MQ=255)
                                                                         gAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTa                                                                       >  2:1416818/1‑148 (MQ=255)
                                                                          aaaGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTAt                                                                      <  2:874333/148‑1 (MQ=255)
                                                                             gACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGa                                                                   <  2:2063533/148‑1 (MQ=255)
                                                                              aCAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGAt                                                                  >  2:1218369/1‑148 (MQ=255)
                                                                              aCAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGAt                                                                  >  1:3190107/1‑148 (MQ=255)
                                                                                  cTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAAc                                                              >  1:3548403/1‑148 (MQ=255)
                                                                                  cTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAAc                                                              >  1:500142/1‑148 (MQ=255)
                                                                                    ggTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCg                                                            >  1:1567363/1‑148 (MQ=255)
                                                                                      tGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGt                                                          <  2:2736375/148‑1 (MQ=255)
                                                                                         tGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGc                                                       >  1:229367/1‑148 (MQ=255)
                                                                                         tGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGc                                                       >  1:3362500/1‑148 (MQ=255)
                                                                                           ggATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCgg                                                     >  2:1854524/1‑148 (MQ=255)
                                                                                           ggATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCgg                                                     >  2:3136127/1‑148 (MQ=255)
                                                                                             aTTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGtt                                                   <  1:3124132/148‑1 (MQ=255)
                                                                                             aTTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGtt                                                   <  1:788333/148‑1 (MQ=255)
                                                                                              ttATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttc                                                  >  2:33979/1‑148 (MQ=255)
                                                                                               tATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttct                                                 <  2:2253144/148‑1 (MQ=255)
                                                                                               tATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttct                                                 >  2:2184743/1‑148 (MQ=255)
                                                                                               tATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttct                                                 >  2:2184557/1‑148 (MQ=255)
                                                                                                 tgatgTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttcttt                                               <  1:668030/148‑1 (MQ=255)
                                                                                                 tgatgTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttcttt                                               <  1:2459565/148‑1 (MQ=255)
                                                                                                  gatgTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttctttc                                              <  2:402125/148‑1 (MQ=255)
                                                                                                    tgTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCta                                            >  2:2332642/1‑148 (MQ=255)
                                                                                                        tattattatGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCtaataa                                        >  1:1501725/4‑148 (MQ=255)
                                                                                                         attattatGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTaataat                                       >  2:620631/3‑148 (MQ=255)
                                                                                                             ttatGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGcc                                   >  2:2300170/1‑148 (MQ=255)
                                                                                                              tatGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGccc                                  <  1:2795877/148‑1 (MQ=255)
                                                                                                              tatGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGccc                                  >  1:3470932/1‑148 (MQ=255)
                                                                                                                 gTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGc                               <  2:479097/148‑1 (MQ=255)
                                                                                                                 gTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGc                               >  2:941541/1‑148 (MQ=255)
                                                                                                                 gTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGc                               >  1:2342320/1‑148 (MQ=255)
                                                                                                                  tataTTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCATTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCa                              >  1:2501979/1‑148 (MQ=255)
                                                                                                                  tataTTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCTCa                              <  2:3396663/148‑1 (MQ=255)
                                                                                                                  tataTTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCa                              >  2:3078383/1‑148 (MQ=255)
                                                                                                                   atatTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAg                             >  2:908246/1‑148 (MQ=255)
                                                                                                                    tatTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCTCTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAgg                            <  1:490446/148‑1 (MQ=255)
                                                                                                                      ttCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGat                          <  2:535701/148‑1 (MQ=255)
                                                                                                                       tCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGata                         >  2:3530408/1‑148 (MQ=255)
                                                                                                                          ggTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATAtct                      >  1:1785584/1‑148 (MQ=255)
                                                                                                                          ggTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATAtct                      >  1:3225098/1‑148 (MQ=255)
                                                                                                                           gtgtGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATAtctt                     <  1:1906342/148‑2 (MQ=255)
                                                                                                                           gtgtGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATAtctc                     <  2:2630277/148‑1 (MQ=255)
                                                                                                                           gtgtGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGGTGCGGTTCTTTCTAATAATCGCCCCGCAGGATAtctt                     <  1:1905927/148‑2 (MQ=255)
                                                                                                                              tgCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTa                  >  2:648313/1‑148 (MQ=255)
                                                                                                                               gCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTaa                 <  1:2161394/148‑1 (MQ=255)
                                                                                                                                cATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAg                >  2:1358030/1‑148 (MQ=255)
                                                                                                                                  ttattaTGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTg              >  2:1596921/1‑148 (MQ=255)
                                                                                                                                  ttattaTGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTg              <  1:3485278/148‑1 (MQ=255)
                                                                                                                                  ttattaTGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTg              <  1:36050/148‑1 (MQ=255)
                                                                                                                                   tattatGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGc             >  2:711669/1‑148 (MQ=255)
                                                                                                                                     ttatGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCa           >  2:2939432/1‑148 (MQ=255)
                                                                                                                                      tatGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAg          <  2:1022059/148‑1 (MQ=255)
                                                                                                                                        tGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAgcg        >  1:3033091/1‑148 (MQ=255)
                                                                                                                                        tGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAgcg        <  2:3197315/148‑1 (MQ=255)
                                                                                                                                         gAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAgcgc       >  2:2734317/1‑148 (MQ=255)
                                                                                                                                          aGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGTTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCa      >  1:2200786/1‑148 (MQ=255)
                                                                                                                                            ggTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCAtt    >  2:3288314/1‑148 (MQ=255)
                                                                                                                                            ggTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCAtt    >  1:1351599/1‑148 (MQ=255)
                                                                                                                                            ggTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCAtt    <  1:1019465/148‑1 (MQ=255)
                                                                                                                                              tataACTGTATGCATCGAATTCTCGCTGAAAAATCGGTGAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCg  >  2:3253455/1‑148 (MQ=255)
                                                                                                                                              tataACTGTATGCATCGAATTCTCGCTGAAAAATCGGTGAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCg  >  2:1298548/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCG  >  CP009273/248341‑248630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCG  >  CP009273/248340‑248635
                                                                                                                                                  |                                                                                                                                                     
CCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACT                                                                                                                                                    <  SRR19973066.2010031/150‑1 (MQ=60)
  TGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGT                                                                                                                                                  >  SRR19973066.3218548/1‑150 (MQ=60)
      ATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTCTAACTGTATGC                                                                                                                                              >  SRR19973066.1717151/1‑150 (MQ=60)
      ATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGC                                                                                                                                              >  SRR19973066.1029568/1‑150 (MQ=60)
      ATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGC                                                                                                                                              >  SRR19973066.1029578/1‑150 (MQ=60)
       TGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCA                                                                                                                                             <  SRR19973066.2406417/150‑1 (MQ=60)
        GAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCAT                                                                                                                                            <  SRR19973066.1162027/150‑1 (MQ=60)
              CGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATT                                                                                                                                      <  SRR19973066.1407301/150‑1 (MQ=60)
                CGGCGGGCGCTGTGTGCGTCTGGTGGGGCTGCATGTTACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTTGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCT                                                                                                                                    <  SRR19973066.2722020/150‑1 (MQ=60)
                CGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCT                                                                                                                                    <  SRR19973066.1268169/150‑1 (MQ=60)
                 GGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTC                                                                                                                                   <  SRR19973066.3005264/150‑1 (MQ=60)
                   CGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGC                                                                                                                                 >  SRR19973066.2839611/1‑150 (MQ=60)
                     GGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGTGTATAACTGTATGCATCGAATTCTCTCTG                                                                                                                               >  SRR19973066.3419328/1‑150 (MQ=60)
                     GGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTG                                                                                                                               >  SRR19973066.3419324/1‑150 (MQ=60)
                       CGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAGAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAA                                                                                                                             <  SRR19973066.1088107/150‑1 (MQ=60)
                       CGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAA                                                                                                                             <  SRR19973066.2878789/150‑1 (MQ=60)
                         CGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAA                                                                                                                           <  SRR19973066.1232334/150‑1 (MQ=60)
                          GGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAA                                                                                                                          <  SRR19973066.865522/150‑1 (MQ=60)
                          GGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAA                                                                                                                          <  SRR19973066.865528/150‑1 (MQ=60)
                            TGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATC                                                                                                                        <  SRR19973066.1596080/150‑1 (MQ=60)
                              TGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGG                                                                                                                      <  SRR19973066.157317/150‑1 (MQ=60)
                              TGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGG                                                                                                                      <  SRR19973066.157484/150‑1 (MQ=60)
                                   CTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAAT                                                                                                                 <  SRR19973066.2686783/150‑1 (MQ=60)
                                   CTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAAT                                                                                                                 <  SRR19973066.2686419/150‑1 (MQ=60)
                                     GGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGACAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATAT                                                                                                               <  SRR19973066.658624/150‑1 (MQ=60)
                                       TGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCA                                                                                                             >  SRR19973066.3240650/1‑150 (MQ=60)
                                        GGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCAC                                                                                                            <  SRR19973066.1207174/150‑1 (MQ=60)
                                                 TGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACG                                                                                                   >  SRR19973066.2161262/1‑150 (MQ=60)
                                                 TGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACG                                                                                                   <  SRR19973066.133564/150‑1 (MQ=60)
                                                  GTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGT                                                                                                  <  SRR19973066.391722/150‑1 (MQ=60)
                                                  GTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGT                                                                                                  <  SRR19973066.3349739/150‑1 (MQ=60)
                                                   TGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTA                                                                                                 >  SRR19973066.2998186/1‑150 (MQ=60)
                                                       GTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAA                                                                                             <  SRR19973066.2028264/150‑1 (MQ=60)
                                                           CTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCA                                                                                         <  SRR19973066.3460172/150‑1 (MQ=60)
                                                           CTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCA                                                                                         >  SRR19973066.248637/1‑150 (MQ=60)
                                                                    CAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATAC                                                                                <  SRR19973066.135261/150‑1 (MQ=60)
                                                                    CAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATAC                                                                                <  SRR19973066.134862/150‑1 (MQ=60)
                                                                       ATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTT                                                                             >  SRR19973066.647576/1‑150 (MQ=60)
                                                                       ATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTT                                                                             <  SRR19973066.217686/150‑1 (MQ=60)
                                                                          GAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATT                                                                          <  SRR19973066.2722528/150‑1 (MQ=60)
                                                                           AAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTG                                                                         <  SRR19973066.465725/150‑1 (MQ=60)
                                                                             AGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGAT                                                                       >  SRR19973066.3211312/1‑150 (MQ=60)
                                                                              GACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATC                                                                      >  SRR19973066.869357/1‑150 (MQ=60)
                                                                                 AACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAAC                                                                   >  SRR19973066.503864/1‑150 (MQ=60)
                                                                                 AACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAAC                                                                   >  SRR19973066.3571957/1‑150 (MQ=60)
                                                                                 AACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAAC                                                                   >  SRR19973066.503857/1‑150 (MQ=60)
                                                                                   CTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCG                                                                 >  SRR19973066.1578654/1‑150 (MQ=60)
                                                                                      GTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTT                                                              <  SRR19973066.1362360/150‑1 (MQ=60)
                                                                                       TGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTG                                                             >  SRR19973066.1461564/1‑150 (MQ=60)
                                                                                        GCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGC                                                            >  SRR19973066.231197/1‑150 (MQ=60)
                                                                                        GCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGC                                                            >  SRR19973066.3384918/1‑150 (MQ=60)
                                                                                         CTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCG                                                           >  SRR19973066.1590661/1‑150 (MQ=60)
                                                                                          TGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGG                                                          >  SRR19973066.1416712/1‑150 (MQ=60)
                                                                                            GGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTT                                                        >  SRR19973066.169066/1‑150 (MQ=60)
                                                                                              ATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCT                                                      <  SRR19973066.3144995/150‑1 (MQ=60)
                                                                                              ATTATGAGGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCT                                                      <  SRR19973066.794143/150‑1 (MQ=60)
                                                                                                 ATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTC                                                   >  SRR19973066.614480/1‑150 (MQ=60)
                                                                                                  TGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCT                                                  <  SRR19973066.2476329/150‑1 (MQ=60)
                                                                                                  TGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCT                                                  <  SRR19973066.672912/150‑1 (MQ=60)
                                                                                                   GATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTA                                                 >  SRR19973066.347165/1‑150 (MQ=60)
                                                                                                    ATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAA                                                >  SRR19973066.3502378/1‑150 (MQ=60)
                                                                                                       TATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAA                                             >  SRR19973066.1512187/1‑150 (MQ=60)
                                                                                                       TATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAA                                             >  SRR19973066.3099431/1‑150 (MQ=60)
                                                                                                        ATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCNGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAAT                                            <  SRR19973066.1823089/150‑1 (MQ=60)
                                                                                                          ATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCG                                          >  SRR19973066.3312970/1‑150 (MQ=60)
                                                                                                          ATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCG                                          >  SRR19973066.194726/1‑150 (MQ=60)
                                                                                                            TATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCC                                        <  SRR19973066.2762745/150‑1 (MQ=60)
                                                                                                             ATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCC                                       >  SRR19973066.2354801/1‑150 (MQ=60)
                                                                                                             ATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCC                                       >  SRR19973066.3494118/1‑150 (MQ=60)
                                                                                                             ATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAAGATCACGGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCC                                       <  SRR19973066.3380870/150‑1 (MQ=60)
                                                                                                              TTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCC                                      <  SRR19973066.155561/150‑1 (MQ=60)
                                                                                                               TATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCG                                     <  SRR19973066.2817138/150‑1 (MQ=60)
                                                                                                               TATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCG                                     <  SRR19973066.2814697/150‑1 (MQ=60)
                                                                                                                ATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGC                                    >  SRR19973066.2710768/1‑150 (MQ=60)
                                                                                                                ATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGC                                    >  SRR19973066.2358268/1‑150 (MQ=60)
                                                                                                                ATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGC                                    <  SRR19973066.1236415/150‑1 (MQ=60)
                                                                                                                 TGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCATTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCA                                   >  SRR19973066.2519022/1‑150 (MQ=60)
                                                                                                                     TATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCTCTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGAT                               <  SRR19973066.494120/150‑1 (MQ=60)
                                                                                                                     TATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGAT                               <  SRR19973066.3145845/150‑1 (MQ=60)
                                                                                                                     TATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGAT                               >  SRR19973066.1233487/1‑150 (MQ=60)
                                                                                                                         CTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCT                           >  SRR19973066.1798323/1‑150 (MQ=60)
                                                                                                                         CTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCT                           >  SRR19973066.3246421/1‑150 (MQ=60)
                                                                                                                            GTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTTTT                        <  SRR19973066.1919593/150‑1 (MQ=60)
                                                                                                                            GTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTTTT                        <  SRR19973066.1919926/150‑1 (MQ=60)
                                                                                                                            GTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGGTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTTTT                        <  SRR19973066.1919510/150‑1 (MQ=60)
                                                                                                                             TGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTA                       >  SRR19973066.1655503/1‑150 (MQ=60)
                                                                                                                               TGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAG                     <  SRR19973066.737120/150‑1 (MQ=60)
                                                                                                                               TGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAG                     >  SRR19973066.2326814/1‑150 (MQ=60)
                                                                                                                                GCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGT                    <  SRR19973066.2176410/150‑1 (MQ=60)
                                                                                                                                GCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGT                    <  SRR19973066.2176642/150‑1 (MQ=60)
                                                                                                                                 CATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTG                   <  SRR19973066.3049408/150‑1 (MQ=60)
                                                                                                                                   TTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCC                 <  SRR19973066.36537/150‑1 (MQ=60)
                                                                                                                                   TTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCC                 <  SRR19973066.3508534/150‑1 (MQ=60)
                                                                                                                                   TTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCC                 <  SRR19973066.3508530/150‑1 (MQ=60)
                                                                                                                                   TTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCC                 <  SRR19973066.36512/150‑1 (MQ=60)
                                                                                                                                     ATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAG               >  SRR19973066.790173/1‑150 (MQ=60)
                                                                                                                                       TATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCG             >  SRR19973066.3053356/1‑150 (MQ=60)
                                                                                                                                         TGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGTTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCA           >  SRR19973066.2216045/1‑150 (MQ=60)
                                                                                                                                         TGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGTTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCA           >  SRR19973066.2216231/1‑150 (MQ=60)
                                                                                                                                           AGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATT         >  SRR19973066.1361388/1‑150 (MQ=60)
                                                                                                                                             GGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCG       <  SRR19973066.1026965/150‑1 (MQ=60)
                                                                                                                                             GGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCG       <  SRR19973066.1780477/150‑1 (MQ=60)
                                                                                                                                              GTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGA      >  SRR19973066.2985681/1‑150 (MQ=60)
                                                                                                                                              GTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGA      >  SRR19973066.1615255/1‑150 (MQ=60)
                                                                                                                                                 TAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGC   >  SRR19973066.1764774/1‑150 (MQ=60)
                                                                                                                                                  AACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCG  >  SRR19973066.1020221/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCG  >  CP009273/248340‑248635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑