Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 248,486 C→A 100% intergenic (+46/‑6) dinB → / → yafN DNA polymerase IV/antitoxin of the YafO‑YafN toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273248,4860CA97.9% 735.3 / 5.9 233intergenic (+46/‑6)dinB/yafNDNA polymerase IV/antitoxin of the YafO‑YafN toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (4/1);  new base A (122/106);  total (126/107)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 235 total aligned reads displayed.

GGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCG  >  CP009273/248343‑248630
                                                                                                                                               |                                                                                                                                                
gggATGAACGCCGCGGCGGGCGCGTTGTTCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTg                                                                                                                                              <  2:2982793/148‑1 (MQ=255)
 ggATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGt                                                                                                                                             <  1:638243/148‑1 (MQ=255)
 ggATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGt                                                                                                                                             <  2:62480/148‑1 (MQ=255)
   aTGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTAt                                                                                                                                           >  2:2840900/1‑148 (MQ=255)
    tGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATg                                                                                                                                          >  2:2961868/1‑148 (MQ=255)
      aaCGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCa                                                                                                                                        >  2:1882479/1‑148 (MQ=255)
       aCGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCAt                                                                                                                                       >  1:134484/1‑148 (MQ=255)
        cgccgcGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATc                                                                                                                                      >  2:304565/1‑148 (MQ=255)
          ccgcGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGa                                                                                                                                    <  1:3409719/148‑1 (MQ=255)
           cgcgGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGaa                                                                                                                                   <  1:1699330/148‑1 (MQ=255)
               gcgggcgCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATtct                                                                                                                               <  2:518429/148‑1 (MQ=255)
                   gcgcGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCt                                                                                                                           >  2:2765811/1‑148 (MQ=255)
                        gtgtGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGaaaa                                                                                                                      <  1:1187450/148‑1 (MQ=255)
                            gCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCg                                                                                                                  <  2:3615865/148‑1 (MQ=255)
                              gTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGt                                                                                                                <  1:2372519/148‑1 (MQ=255)
                              gTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGt                                                                                                                <  1:3560474/148‑1 (MQ=255)
                               tCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTc                                                                                                               >  2:3025073/1‑148 (MQ=255)
                                  ggtggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAat                                                                                                            <  1:1325839/148‑1 (MQ=255)
                                  ggtggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAat                                                                                                            <  2:2383306/148‑1 (MQ=255)
                                  ggtggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAat                                                                                                            <  1:2067385/148‑1 (MQ=255)
                                  ggtggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAat                                                                                                            >  1:949150/1‑148 (MQ=255)
                                   gtggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAata                                                                                                           <  1:1426390/148‑1 (MQ=255)
                                      gggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCa                                                                                                        <  2:418906/148‑1 (MQ=255)
                                      gggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCa                                                                                                        <  1:983864/148‑1 (MQ=255)
                                      gggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCa                                                                                                        >  2:3173583/1‑148 (MQ=255)
                                      gggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCa                                                                                                        <  1:113208/148‑1 (MQ=255)
                                      gggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCa                                                                                                        >  1:2095339/1‑148 (MQ=255)
                                       ggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCAc                                                                                                       <  1:310755/148‑1 (MQ=255)
                                       ggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCAc                                                                                                       >  1:1295492/1‑148 (MQ=255)
                                         cTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTg                                                                                                     <  2:720146/148‑1 (MQ=255)
                                           gCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAg                                                                                                   >  2:2154702/1‑148 (MQ=255)
                                           gCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAg                                                                                                   >  2:590869/1‑148 (MQ=255)
                                                 gACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGAAGTATATTATTATGTATATTCTGGTGTGCATTAATATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGt                                                                                             >  1:3032651/1‑148 (MQ=255)
                                                     ttGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTaaaa                                                                                         >  2:335719/1‑148 (MQ=255)
                                                        cTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAAcc                                                                                      <  1:3162999/148‑1 (MQ=255)
                                                          tGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCa                                                                                    >  2:243539/1‑148 (MQ=255)
                                                           gACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAg                                                                                   <  2:3783715/148‑1 (MQ=255)
                                                           gACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAg                                                                                   <  2:2187681/148‑1 (MQ=255)
                                                               cGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTaa                                                                               <  1:2452598/148‑1 (MQ=255)
                                                               cGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTaa                                                                               <  1:1605705/148‑1 (MQ=255)
                                                                    aTGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACt                                                                          >  2:1112616/1‑148 (MQ=255)
                                                                      ggAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACttt                                                                        >  1:3536898/1‑148 (MQ=255)
                                                                      ggAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACttt                                                                        <  1:2808798/148‑1 (MQ=255)
                                                                      ggAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACttt                                                                        <  1:1507149/148‑1 (MQ=255)
                                                                       gAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTa                                                                       <  2:2658981/148‑1 (MQ=255)
                                                                        aaaGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTAt                                                                      >  1:3446037/1‑148 (MQ=255)
                                                                        aaaGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTAt                                                                      >  2:337085/1‑148 (MQ=255)
                                                                          aGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTg                                                                    >  2:1158691/1‑148 (MQ=255)
                                                                           gACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGa                                                                   >  2:3687424/1‑148 (MQ=255)
                                                                           gACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGa                                                                   >  1:2253273/1‑148 (MQ=255)
                                                                           gACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGa                                                                   >  1:301316/1‑148 (MQ=255)
                                                                           gACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGa                                                                   <  2:1569197/148‑1 (MQ=255)
                                                                           gACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGa                                                                   <  1:2154730/148‑1 (MQ=255)
                                                                              aaCTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCa                                                                <  2:2095339/148‑1 (MQ=255)
                                                                               aCTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCaa                                                               >  1:1305448/1‑148 (MQ=255)
                                                                                   gTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCgg                                                           >  1:2332145/1‑148 (MQ=255)
                                                                                       tGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGc                                                       >  2:3492271/1‑148 (MQ=255)
                                                                                        gggATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCg                                                      >  2:2708943/1‑148 (MQ=255)
                                                                                          gATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGt                                                    >  2:1404391/1‑148 (MQ=255)
                                                                                           aTTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGtt                                                   <  1:2841211/148‑1 (MQ=255)
                                                                                           aTTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGtt                                                   <  2:3201857/148‑1 (MQ=255)
                                                                                             tATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGttct                                                 >  2:2508715/1‑148 (MQ=255)
                                                                                                   gTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCtaa                                           >  2:1591442/1‑148 (MQ=255)
                                                                                                    tataTTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATAGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATAATTTATTGATCAACCGGTTGCGGTTCTTTCtaat                                          >  2:3513691/1‑148 (MQ=255)
                                                                                                     atatTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCtaata                                         >  1:1802511/1‑148 (MQ=255)
                                                                                                     atatTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCtaata                                         <  2:10431/148‑1 (MQ=255)
                                                                                                      tattattatGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCtaataa                                        >  2:3579516/4‑148 (MQ=255)
                                                                                                            tatGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGccc                                  >  2:2620710/1‑148 (MQ=255)
                                                                                                               gTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGc                               >  2:1398739/1‑148 (MQ=255)
                                                                                                               gTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGc                               >  1:416160/1‑148 (MQ=255)
                                                                                                                 atatTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAg                             >  2:2303116/1‑148 (MQ=255)
                                                                                                                 atatTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAg                             >  2:3373860/1‑148 (MQ=255)
                                                                                                                   atTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGa                           >  1:546960/1‑148 (MQ=255)
                                                                                                                   atTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGa                           <  2:3262831/148‑1 (MQ=255)
                                                                                                                     tCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGata                         >  1:2427497/1‑148 (MQ=255)
                                                                                                                       tGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATAtc                       >  2:939254/1‑148 (MQ=255)
                                                                                                                        ggTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATAtct                      <  2:2081541/148‑1 (MQ=255)
                                                                                                                        ggTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATAtct                      >  2:1905619/1‑148 (MQ=255)
                                                                                                                         gtgtGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATAtctc                     <  2:2066027/148‑1 (MQ=255)
                                                                                                                          tgtgCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATctct                    <  1:243539/148‑1 (MQ=255)
                                                                                                                            tgCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTa                  <  1:1010451/148‑1 (MQ=255)
                                                                                                                               attattATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGt               >  1:61077/1‑148 (MQ=255)
                                                                                                                                ttattaTGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCACCGCAGGATATCTCTTAAGTg              >  2:1017479/1‑148 (MQ=255)
                                                                                                                                 tattatGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGc             <  1:1165957/148‑1 (MQ=255)
                                                                                                                                  attatGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGcc            >  2:114795/1‑148 (MQ=255)
                                                                                                                                   ttatGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCa           >  1:1495364/1‑148 (MQ=255)
                                                                                                                                   ttatGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACATTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCa           >  1:833580/1‑148 (MQ=255)
                                                                                                                                    tatGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAg          >  2:2967419/1‑148 (MQ=255)
                                                                                                                                    tatGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAg          >  1:3695626/1‑148 (MQ=255)
                                                                                                                                       gAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAgcgc       >  1:3667572/1‑148 (MQ=255)
                                                                                                                                        aGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCa      >  2:3662886/1‑148 (MQ=255)
                                                                                                                                        aGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCa      <  1:271153/148‑1 (MQ=255)
                                                                                                                                         gggTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCAt     >  1:3639515/1‑148 (MQ=255)
                                                                                                                                         gggTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCAt     >  1:3308944/1‑148 (MQ=255)
                                                                                                                                         gggTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCAt     >  2:1672438/1‑148 (MQ=255)
                                                                                                                                          ggTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCAtt    >  2:3786584/1‑148 (MQ=255)
                                                                                                                                          ggTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCAtt    >  2:1445144/1‑148 (MQ=255)
                                                                                                                                          ggTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCAtt    <  2:1059685/148‑1 (MQ=255)
                                                                                                                                           gTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTc   >  2:1330996/1‑148 (MQ=255)
                                                                                                                                            tataACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCg  >  1:472820/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCG  >  CP009273/248343‑248630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCG  >  CP009273/248339‑248635
                                                                                                                                                   |                                                                                                                                                     
ACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAAC                                                                                                                                                     <  SRR19973069.63460/150‑1 (MQ=60)
 CCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACT                                                                                                                                                    <  SRR19973069.157902/150‑1 (MQ=60)
     GGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTAT                                                                                                                                                <  SRR19973069.642443/150‑1 (MQ=60)
       ATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCACAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGC                                                                                                                                              >  SRR19973069.1672374/1‑150 (MQ=60)
         GAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCAT                                                                                                                                            >  SRR19973069.135736/1‑150 (MQ=60)
         GAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCAT                                                                                                                                            >  SRR19973069.500245/1‑150 (MQ=60)
             GCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAA                                                                                                                                        <  SRR19973069.2639698/150‑1 (MQ=60)
              CCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAAT                                                                                                                                       <  SRR19973069.3431939/150‑1 (MQ=60)
              CCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAAT                                                                                                                                       <  SRR19973069.2770906/150‑1 (MQ=60)
               CGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATT                                                                                                                                      <  SRR19973069.1711220/150‑1 (MQ=60)
                        CGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAA                                                                                                                             >  SRR19973069.210485/1‑150 (MQ=60)
                            GTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAAT                                                                                                                         <  SRR19973069.1194674/150‑1 (MQ=60)
                            GTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAAT                                                                                                                         <  SRR19973069.1195648/150‑1 (MQ=60)
                             TGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATC                                                                                                                        <  SRR19973069.3555781/150‑1 (MQ=60)
                                  GTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCA                                                                                                                   <  SRR19973069.2388504/150‑1 (MQ=60)
                                  GTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCA                                                                                                                   <  SRR19973069.3583652/150‑1 (MQ=60)
                                  GTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCA                                                                                                                   <  SRR19973069.2388616/150‑1 (MQ=60)
                                    CTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAAT                                                                                                                 >  SRR19973069.955537/1‑150 (MQ=60)
                                      GGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATAT                                                                                                               <  SRR19973069.2081714/150‑1 (MQ=60)
                                      GGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATAT                                                                                                               <  SRR19973069.1334957/150‑1 (MQ=60)
                                      GGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATAT                                                                                                               <  SRR19973069.1335188/150‑1 (MQ=60)
                                       GTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATC                                                                                                              <  SRR19973069.1436356/150‑1 (MQ=60)
                                        TGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCA                                                                                                             >  SRR19973069.2109822/1‑150 (MQ=60)
                                        TGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCA                                                                                                             >  SRR19973069.2109782/1‑150 (MQ=60)
                                        TGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATACTCGCTGAAAAATCGGTCAATATCA                                                                                                             >  SRR19973069.3228854/1‑150 (MQ=60)
                                         GNGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCAC                                                                                                            >  SRR19973069.1305761/1‑150 (MQ=60)
                                         GGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCAC                                                                                                            >  SRR19973069.1304214/1‑150 (MQ=60)
                                          GGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACT                                                                                                           <  SRR19973069.114338/150‑1 (MQ=60)
                                          GGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACT                                                                                                           <  SRR19973069.114314/150‑1 (MQ=60)
                                          GGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACT                                                                                                           <  SRR19973069.986277/150‑1 (MQ=60)
                                          GGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACT                                                                                                           <  SRR19973069.990519/150‑1 (MQ=60)
                                           GGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTG                                                                                                          <  SRR19973069.312834/150‑1 (MQ=60)
                                           GGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTG                                                                                                          <  SRR19973069.991835/150‑1 (MQ=60)
                                              TGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGT                                                                                                       >  SRR19973069.1841252/1‑150 (MQ=60)
                                                  TGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACG                                                                                                   <  SRR19973069.305010/150‑1 (MQ=60)
                                                   GTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGT                                                                                                  >  SRR19973069.2206991/1‑150 (MQ=60)
                                                   GTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGAAGTATATTATTATGTATATTCTGGTGTGCATTAATATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGT                                                                                                  >  SRR19973069.3052758/1‑150 (MQ=60)
                                                            CTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCA                                                                                         <  SRR19973069.3183630/150‑1 (MQ=60)
                                                            CTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCA                                                                                         <  SRR19973069.1175629/150‑1 (MQ=60)
                                                             TTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAG                                                                                        <  SRR19973069.2998299/150‑1 (MQ=60)
                                                             TTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAG                                                                                        >  SRR19973069.1509410/1‑150 (MQ=60)
                                                               GACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCT                                                                                      <  SRR19973069.3736092/150‑1 (MQ=60)
                                                                   CGCAAATGGAAAGACATCTGGTGCTGGGATTATGATTTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAAT                                                                                  <  SRR19973069.2469086/150‑1 (MQ=60)
                                                                   CGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAAT                                                                                  <  SRR19973069.1616509/150‑1 (MQ=60)
                                                                   CGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAAT                                                                                  <  SRR19973069.2469092/150‑1 (MQ=60)
                                                                      AAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACT                                                                               <  SRR19973069.1749639/150‑1 (MQ=60)
                                                                      AAATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACT                                                                               <  SRR19973069.3652150/150‑1 (MQ=60)
                                                                       AATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTT                                                                              >  SRR19973069.3733058/1‑150 (MQ=60)
                                                                        ATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTT                                                                             <  SRR19973069.648635/150‑1 (MQ=60)
                                                                        ATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTT                                                                             <  SRR19973069.648616/150‑1 (MQ=60)
                                                                        ATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTT                                                                             >  SRR19973069.3559833/1‑150 (MQ=60)
                                                                        ATGGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTT                                                                             >  SRR19973069.1067053/1‑150 (MQ=60)
                                                                          GGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTAT                                                                           <  SRR19973069.1517582/150‑1 (MQ=60)
                                                                          GGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTAT                                                                           <  SRR19973069.2827416/150‑1 (MQ=60)
                                                                          GGAAAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTAT                                                                           >  SRR19973069.3468392/1‑150 (MQ=60)
                                                                             AAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGA                                                                        >  SRR19973069.2268489/1‑150 (MQ=60)
                                                                             AAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGA                                                                        >  SRR19973069.303359/1‑150 (MQ=60)
                                                                             AAGACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATACTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGA                                                                        >  SRR19973069.2268727/1‑150 (MQ=60)
                                                                               GACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATC                                                                      <  SRR19973069.2169485/150‑1 (MQ=60)
                                                                               GACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATC                                                                      <  SRR19973069.2169457/150‑1 (MQ=60)
                                                                                ACAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCA                                                                     <  SRR19973069.2405894/150‑1 (MQ=60)
                                                                                 CAACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAA                                                                    >  SRR19973069.1314331/1‑150 (MQ=60)
                                                                                  AACTGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAAC                                                                   <  SRR19973069.3616925/150‑1 (MQ=60)
                                                                                     TGGTGCTGGGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGG                                                                >  SRR19973069.2348021/1‑150 (MQ=60)
                                                                                             GGATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTT                                                        >  SRR19973069.3665643/1‑150 (MQ=60)
                                                                                              GATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTC                                                       >  SRR19973069.2279802/1‑150 (MQ=60)
                                                                                               ATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCT                                                      <  SRR19973069.2859955/150‑1 (MQ=60)
                                                                                               ATTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCT                                                      <  SRR19973069.2859643/150‑1 (MQ=60)
                                                                                                TTATGATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTAGAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTT                                                     <  SRR19973069.1355204/150‑1 (MQ=60)
                                                                                                    GATGTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTA                                                 <  SRR19973069.3711539/150‑1 (MQ=60)
                                                                                                       GTATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATA                                              >  SRR19973069.1815199/1‑150 (MQ=60)
                                                                                                         ATATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAAT                                            >  SRR19973069.3560280/1‑150 (MQ=60)
                                                                                                           ATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCG                                          >  SRR19973069.477772/1‑150 (MQ=60)
                                                                                                           ATTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCG                                          >  SRR19973069.2414143/1‑150 (MQ=60)
                                                                                                             TATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTTAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCC                                        >  SRR19973069.1448978/1‑150 (MQ=60)
                                                                                                              ATTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCC                                       >  SRR19973069.1326930/1‑150 (MQ=60)
                                                                                                               TTATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCC                                      >  SRR19973069.3067420/1‑150 (MQ=60)
                                                                                                                TATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCG                                     <  SRR19973069.575562/150‑1 (MQ=60)
                                                                                                                 ATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGC                                    >  SRR19973069.3456495/1‑150 (MQ=60)
                                                                                                                 ATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGC                                    >  SRR19973069.419212/1‑150 (MQ=60)
                                                                                                                 ATGTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGC                                    >  SRR19973069.3158237/1‑150 (MQ=60)
                                                                                                                   GTATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAG                                  >  SRR19973069.132917/1‑150 (MQ=60)
                                                                                                                    TATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGG                                 <  SRR19973069.1586589/150‑1 (MQ=60)
                                                                                                                     ATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGA                                >  SRR19973069.550797/1‑150 (MQ=60)
                                                                                                                     ATATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGA                                >  SRR19973069.2481782/1‑150 (MQ=60)
                                                                                                                      TATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATAAATGAGTTACGTAAAAACCCAGCTAAATACTTTATTTATCAACCGGTTGCGGTTCTTTCTAATAATCTACCCGCAGTAT                               >  SRR19973069.3157259/1‑150 (MQ=60)
                                                                                                                       ATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATA                              >  SRR19973069.2443875/1‑150 (MQ=60)
                                                                                                                       ATTCTGGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATAACACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATA                              >  SRR19973069.2444356/1‑150 (MQ=60)
                                                                                                                            GGTGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGAAAAAACCAAGCTAAATACTATATTGATCAACAGGTTGCGGAACTTTCTAATAATCTCCCCGCAGTATATCTCT                         >  SRR19973069.1283344/1‑150 (MQ=60)
                                                                                                                              TGTGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTA                       <  SRR19973069.245356/150‑1 (MQ=60)
                                                                                                                                TGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAG                     >  SRR19973069.2985454/1‑150 (MQ=60)
                                                                                                                                TGCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAG                     <  SRR19973069.1017413/150‑1 (MQ=60)
                                                                                                                                 GCATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGT                    >  SRR19973069.61803/1‑150 (MQ=60)
                                                                                                                                   ATTATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGC                  >  SRR19973069.628638/1‑150 (MQ=60)
                                                                                                                                     TATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCA                >  SRR19973069.1505735/1‑150 (MQ=60)
                                                                                                                                     TATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCA                <  SRR19973069.1174059/150‑1 (MQ=60)
                                                                                                                                     TATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCA                <  SRR19973069.1498440/150‑1 (MQ=60)
                                                                                                                                     TATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCA                <  SRR19973069.3415575/150‑1 (MQ=60)
                                                                                                                                     TATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACATTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCA                >  SRR19973069.839450/1‑150 (MQ=60)
                                                                                                                                      ATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAG               >  SRR19973069.3719785/1‑150 (MQ=60)
                                                                                                                                      ATTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAG               >  SRR19973069.86661/1‑150 (MQ=60)
                                                                                                                                       TTATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGC              <  SRR19973069.1641249/150‑1 (MQ=60)
                                                                                                                                         ATGAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGC            >  SRR19973069.3691575/1‑150 (MQ=60)
                                                                                                                                           GAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCAT          >  SRR19973069.3663359/1‑150 (MQ=60)
                                                                                                                                           GAGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCAT          >  SRR19973069.3330622/1‑150 (MQ=60)
                                                                                                                                            AGGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATT         <  SRR19973069.273076/150‑1 (MQ=60)
                                                                                                                                             GGGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGTTTCTTTCTAATAATCGCCCCGCAGGATATATCTTAAGTGCCAGAGCATTC        >  SRR19973069.3464913/1‑150 (MQ=60)
                                                                                                                                              GGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCG       >  SRR19973069.476259/1‑150 (MQ=60)
                                                                                                                                              GGTATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCG       >  SRR19973069.475756/1‑150 (MQ=60)
                                                                                                                                                TATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAA     >  SRR19973069.822564/1‑150 (MQ=60)
                                                                                                                                                 ATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAG    <  SRR19973069.3796517/150‑1 (MQ=60)
                                                                                                                                                 ATAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAG    <  SRR19973069.1895554/150‑1 (MQ=60)
                                                                                                                                                 ATAACTGGATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAG    <  SRR19973069.2743355/150‑1 (MQ=60)
                                                                                                                                                  TAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGC   <  SRR19973069.2388753/150‑1 (MQ=60)
                                                                                                                                                  TAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGC   >  SRR19973069.674065/1‑150 (MQ=60)
                                                                                                                                                  TAACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGC   <  SRR19973069.1330246/150‑1 (MQ=60)
                                                                                                                                                   AACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCG  <  SRR19973069.1114271/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
ACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACCGGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCG  >  CP009273/248339‑248635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑