Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,116,964 A→G 100% intergenic (‑21/+53) dinI ← / ← pyrC DNA damage‑inducible protein I/dihydro‑orotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,116,9640AG99.6% 879.7 / ‑5.9 224intergenic (‑21/+53)dinI/pyrCDNA damage‑inducible protein I/dihydro‑orotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (112/109);  minor base T (3/0);  total (115/109)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 225 total aligned reads displayed.

ACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCT  >  CP009273/1116819‑1117108
                                                                                                                                                 |                                                                                                                                                
aCCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCt                                                                                                                                                >  2:261114/1‑148 (MQ=255)
 ccGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCGATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTg                                                                                                                                               >  2:1953616/1‑148 (MQ=255)
  cGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTgg                                                                                                                                              <  2:418219/148‑1 (MQ=255)
  cGATACGTGGACTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCATGGCGTCAATAGCCCCAGCTGGCAATTGATAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTgg                                                                                                                                              <  1:3496155/148‑1 (MQ=255)
    aTACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGtt                                                                                                                                            <  2:179769/148‑1 (MQ=255)
     tACGTGGCCTTCATTATCAGGAATCGCATACTGAATCCGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGtta                                                                                                                                           <  1:3535830/148‑1 (MQ=255)
     tACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGACAGCCAGGGCGTCAATAGCCCCATCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGtta                                                                                                                                           <  1:2215485/148‑1 (MQ=255)
      aCGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGttat                                                                                                                                          <  2:2205668/148‑1 (MQ=255)
      aCGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGttat                                                                                                                                          <  2:2205181/148‑1 (MQ=255)
      aCGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGttat                                                                                                                                          >  1:3756767/1‑148 (MQ=255)
        gTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGttattt                                                                                                                                        <  2:3616898/148‑1 (MQ=255)
         tGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATTGTGACTTCAATTAGCATAATAGCCCCCAGTTGAATATGCTGGttattta                                                                                                                                       >  1:3662597/1‑148 (MQ=255)
           gCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTtata                                                                                                                                     <  1:2913552/148‑1 (MQ=255)
           gCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTtata                                                                                                                                     <  1:3370007/148‑1 (MQ=255)
            ccTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAAATCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATAc                                                                                                                                    >  1:1846799/1‑148 (MQ=255)
             cTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACa                                                                                                                                   <  1:3587953/148‑1 (MQ=255)
             cTTCATGATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACa                                                                                                                                   <  2:2781841/148‑1 (MQ=255)
              ttCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAg                                                                                                                                  >  2:3396345/1‑148 (MQ=255)
               tCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAgg                                                                                                                                 <  2:2487447/148‑1 (MQ=255)
               tCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAgg                                                                                                                                 <  2:2004015/148‑1 (MQ=255)
                  ttATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATATCCCCCTGTTGAATATGCTGGTTATTTATACAGGTaa                                                                                                                              >  1:2824036/1‑148 (MQ=255)
                  ttATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTGTTCGCTATGGTGACTTCAAGTCGCCTAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAgggcc                                                                                                                              >  1:3392638/1‑145 (MQ=255)
                     tCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAAt                                                                                                                           <  1:2439604/148‑1 (MQ=255)
                      cAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATa                                                                                                                          <  2:2067650/148‑1 (MQ=255)
                      cAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATa                                                                                                                          <  2:671997/148‑1 (MQ=255)
                      cAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATa                                                                                                                          <  1:1623034/148‑1 (MQ=255)
                      cAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATAAAGGTAAAATa                                                                                                                          >  1:937516/1‑148 (MQ=255)
                        ggAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAc                                                                                                                        <  1:728055/148‑1 (MQ=255)
                        ggAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAc                                                                                                                        >  2:1417087/1‑148 (MQ=255)
                        ggAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAc                                                                                                                        >  2:3021401/1‑148 (MQ=255)
                         gAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAcc                                                                                                                       <  1:3300477/148‑1 (MQ=255)
                           aaCGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTa                                                                                                                     >  1:561485/1‑148 (MQ=255)
                            aCGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATTCTGGTTATTTATACAGGTAAAATAACCTaa                                                                                                                    >  2:2295535/1‑148 (MQ=255)
                            aCGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTaa                                                                                                                    <  2:1894792/148‑1 (MQ=255)
                            aCGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTaa                                                                                                                    >  2:597820/1‑148 (MQ=255)
                             cGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAAt                                                                                                                   >  2:2045656/1‑148 (MQ=255)
                                 tACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGaca                                                                                                               <  2:1768567/148‑1 (MQ=255)
                                 tACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGaca                                                                                                               <  2:3660654/148‑1 (MQ=255)
                                  aCTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATATCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGacaa                                                                                                              >  1:492520/1‑148 (MQ=255)
                                  aCTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGacaa                                                                                                              >  1:492322/1‑148 (MQ=255)
                                  aCTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGacaa                                                                                                              >  1:249477/1‑148 (MQ=255)
                                  aCTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGacaa                                                                                                              <  1:2999768/148‑1 (MQ=255)
                                        tACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGaa                                                                                                        >  2:3195376/1‑148 (MQ=255)
                                         aCGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAg                                                                                                       <  1:2872605/148‑1 (MQ=255)
                                           ggcggGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCt                                                                                                     <  1:2604794/148‑1 (MQ=255)
                                                gAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAt                                                                                                <  2:466417/148‑1 (MQ=255)
                                                 aaaGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAtt                                                                                               <  2:1334871/148‑1 (MQ=255)
                                                  aaGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAttt                                                                                              <  1:3593229/148‑1 (MQ=255)
                                                  aaGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAttt                                                                                              <  1:3593935/148‑1 (MQ=255)
                                                  aaGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAttt                                                                                              <  2:290585/148‑1 (MQ=255)
                                                    gTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAttttt                                                                                            <  1:3765241/148‑1 (MQ=255)
                                                     ttCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTa                                                                                           <  1:663717/148‑1 (MQ=255)
                                                     ttCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTa                                                                                           <  1:3779214/148‑1 (MQ=255)
                                                     ttCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTa                                                                                           <  2:1216236/148‑1 (MQ=255)
                                                       cGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTAtt                                                                                         <  2:2248654/148‑1 (MQ=255)
                                                          cagccagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGtt                                                                                      <  1:3659962/148‑1 (MQ=255)
                                                                gggCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATATCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACgg                                                                                >  1:255800/1‑148 (MQ=255)
                                                                gggCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTTTTGTTTAACgg                                                                                >  1:2711498/1‑148 (MQ=255)
                                                                 ggCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAAGGa                                                                               >  2:3360037/1‑148 (MQ=255)
                                                                  gCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGAc                                                                              <  1:161698/148‑1 (MQ=255)
                                                                   cGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGAcc                                                                             >  1:1262399/1‑148 (MQ=255)
                                                                        aTAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAACTAATGACAACAGGAAGCTACGATTTTTATTTTTTTACGGACCAGCGt                                                                        >  1:2951604/1‑148 (MQ=255)
                                                                        aTAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTAGCATAATATCCCCCTGTTGTATATGCTGGTTATTTATACAGGTAAAATAACCTAATTACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGt                                                                        >  1:1666009/1‑148 (MQ=255)
                                                                         tAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTa                                                                       <  2:450747/148‑1 (MQ=255)
                                                                         tAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTa                                                                       <  1:2751292/148‑1 (MQ=255)
                                                                              ccAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGtt                                                                  >  2:1171992/1‑148 (MQ=255)
                                                                              ccAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGGTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGACGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGtt                                                                  >  2:2464/1‑148 (MQ=255)
                                                                               cAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGttt                                                                 <  2:2583665/148‑1 (MQ=255)
                                                                                aGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTc                                                                <  1:2287465/148‑1 (MQ=255)
                                                                                aGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTc                                                                <  1:2290319/148‑1 (MQ=255)
                                                                                aGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTc                                                                <  1:3213998/148‑1 (MQ=255)
                                                                                            agaAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGaa                                                    <  1:1354663/148‑1 (MQ=255)
                                                                                            agaAGTTTTCGCTATGGTGACTTCAAATCGCATAAAAGCCCCCAGTTGAATATTCTGGTTATTTATACAGGTAAAATAACCTAATGACAACACGAAGCTACCATTTTTATTGTTTAAAGGACCAGCGTACCGTTTACCCCGCGAggca                                                    >  1:3054150/1‑146 (MQ=255)
                                                                                              aaGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATg                                                  >  1:1630840/1‑148 (MQ=255)
                                                                                                  tttCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCAc                                              >  2:1555280/1‑148 (MQ=255)
                                                                                                       cTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCg                                         >  1:3077336/1‑148 (MQ=255)
                                                                                                          tGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCgtgt                                      <  1:2354638/148‑1 (MQ=255)
                                                                                                           ggTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGACTGGCACCAGCGTGtc                                     >  1:73288/1‑148 (MQ=255)
                                                                                                            gTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGtca                                    >  2:3251522/1‑148 (MQ=255)
                                                                                                              gACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGtcatc                                  >  1:2171277/1‑148 (MQ=255)
                                                                                                                   cAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAtcagtca                             >  2:3141197/1‑148 (MQ=255)
                                                                                                                    aaTTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAtcagtcag                            <  1:2482557/148‑1 (MQ=255)
                                                                                                                      ttCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTg                          >  2:1539628/1‑148 (MQ=255)
                                                                                                                       tCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGc                         >  2:2544963/1‑148 (MQ=255)
                                                                                                                       tCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGc                         >  2:2619243/1‑148 (MQ=255)
                                                                                                                       tCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTAATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTTTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGc                         >  2:1746202/1‑148 (MQ=255)
                                                                                                                            taataGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGc                    >  1:1187339/1‑148 (MQ=255)
                                                                                                                             aataGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACATGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCACCGGCGAGGAATGGCACCATCGTGTCATCAGTCAGTGCGATGCt                   <  2:477638/148‑1 (MQ=255)
                                                                                                                             aataGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCt                   >  2:3043586/1‑148 (MQ=255)
                                                                                                                             aataGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCt                   >  2:2093735/1‑148 (MQ=255)
                                                                                                                             aataGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCt                   >  1:827751/1‑148 (MQ=255)
                                                                                                                             aataGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCt                   >  1:1390973/1‑148 (MQ=255)
                                                                                                                               taGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGACTGGCACCAGCGTGTCATCAGTCAGTGCGATGCttt                 >  2:1202122/1‑148 (MQ=255)
                                                                                                                               taGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCttt                 >  2:2291549/1‑148 (MQ=255)
                                                                                                                                  cccccTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAg              >  2:2423709/1‑148 (MQ=255)
                                                                                                                                        gTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCt        >  2:2052160/1‑148 (MQ=255)
                                                                                                                                         ttGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTg       >  1:2948965/1‑148 (MQ=255)
                                                                                                                                         ttGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTg       <  2:234495/148‑1 (MQ=255)
                                                                                                                                            aaTATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTg    <  1:2045656/148‑1 (MQ=255)
                                                                                                                                              tatgCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGct  >  1:1831242/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCT  >  CP009273/1116819‑1117108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCA  >  CP009273/1116816‑1117113
                                                                                                                                                    |                                                                                                                                                     
CGTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGC                                                                                                                                                      >  SRR19973069.252304/1‑150 (MQ=60)
 GTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCT                                                                                                                                                     <  SRR19973069.1019510/150‑1 (MQ=60)
 GTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCT                                                                                                                                                     <  SRR19973069.1808413/150‑1 (MQ=60)
 GTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCT                                                                                                                                                     <  SRR19973069.1000274/150‑1 (MQ=60)
     CGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTT                                                                                                                                                 <  SRR19973069.1698890/150‑1 (MQ=60)
     CGATACGTGGACTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCATGGCGTCAATAGCCCCAGCTGGCAATTGATAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTT                                                                                                                                                 <  SRR19973069.3518683/150‑1 (MQ=60)
       ATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCGATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTAT                                                                                                                                               >  SRR19973069.3781202/1‑150 (MQ=60)
        TACGTGGCCTTCATTATCAGGAATCGCATACTGAATCCGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATT                                                                                                                                              <  SRR19973069.3558752/150‑1 (MQ=60)
        TACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGACAGCCAGGGCGTCAATAGCCCCATCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATT                                                                                                                                              <  SRR19973069.2230485/150‑1 (MQ=60)
          CGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATTGTGACTTCAATTAGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTA                                                                                                                                            >  SRR19973069.3686576/1‑150 (MQ=60)
          CGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTA                                                                                                                                            >  SRR19973069.3686465/1‑150 (MQ=60)
           GTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTAT                                                                                                                                           <  SRR19973069.3785031/150‑1 (MQ=60)
             GGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATATCCCCCAGTTGAATATGCTGGTTATTTATAC                                                                                                                                         >  SRR19973069.3613933/1‑150 (MQ=60)
             GGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTAGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATAC                                                                                                                                         >  SRR19973069.1403340/1‑150 (MQ=60)
             GGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAAATCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATAC                                                                                                                                         >  SRR19973069.1859703/1‑150 (MQ=60)
             GGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTACCTATCGTGTCGTCAATTGCCATAATATCACCCTTTTGAATATGATGGTTATTTATAC                                                                                                                                         >  SRR19973069.3558858/1‑150 (MQ=60)
              GCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACA                                                                                                                                        <  SRR19973069.3392040/150‑1 (MQ=60)
              GCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACA                                                                                                                                        <  SRR19973069.2932802/150‑1 (MQ=60)
              GCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACA                                                                                                                                        <  SRR19973069.2932829/150‑1 (MQ=60)
              GCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACA                                                                                                                                        <  SRR19973069.1218426/150‑1 (MQ=60)
               CCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAG                                                                                                                                       <  SRR19973069.2987834/150‑1 (MQ=60)
                CTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGG                                                                                                                                      <  SRR19973069.3611357/150‑1 (MQ=60)
                   CATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATATCCCCCAGTTGTATATGCTGGTTATTTATACAGGTAA                                                                                                                                   >  SRR19973069.2842710/1‑150 (MQ=60)
                   CATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAA                                                                                                                                   >  SRR19973069.3414845/1‑150 (MQ=60)
                   CATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTGTTCGCTATGGTGACTTCAAGTCGCCTAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGgcc                                                                                                                                   >  SRR19973069.3414790/1‑147 (MQ=60)
                       ATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATA                                                                                                                               >  SRR19973069.3634051/1‑150 (MQ=60)
                       ATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATAAAGGTAAAATA                                                                                                                               >  SRR19973069.943860/1‑150 (MQ=60)
                       ATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATAAAGGTAAAATA                                                                                                                               >  SRR19973069.129692/1‑150 (MQ=60)
                       ATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATAAAGGTAAAATA                                                                                                                               >  SRR19973069.1731231/1‑150 (MQ=60)
                        TCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAA                                                                                                                              <  SRR19973069.2456039/150‑1 (MQ=60)
                         CAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAC                                                                                                                             <  SRR19973069.1634055/150‑1 (MQ=60)
                          AGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACC                                                                                                                            <  SRR19973069.253005/150‑1 (MQ=60)
                           GGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCT                                                                                                                           <  SRR19973069.733200/150‑1 (MQ=60)
                            GAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTA                                                                                                                          >  SRR19973069.565400/1‑150 (MQ=60)
                            GAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTA                                                                                                                          <  SRR19973069.3322092/150‑1 (MQ=60)
                            GAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTA                                                                                                                          >  SRR19973069.2497176/1‑150 (MQ=60)
                                   ATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATATCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAA                                                                                                                   >  SRR19973069.496064/1‑150 (MQ=60)
                                   ATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAA                                                                                                                   >  SRR19973069.495865/1‑150 (MQ=60)
                                   ATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAA                                                                                                                   >  SRR19973069.251313/1‑150 (MQ=60)
                                   ATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGGTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGTCAA                                                                                                                   >  SRR19973069.495952/1‑150 (MQ=60)
                                     ACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACA                                                                                                                 <  SRR19973069.3019673/150‑1 (MQ=60)
                                      CTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAG                                                                                                                <  SRR19973069.267660/150‑1 (MQ=60)
                                      CTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTAAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAG                                                                                                                <  SRR19973069.1672206/150‑1 (MQ=60)
                                       TGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGG                                                                                                               <  SRR19973069.926561/150‑1 (MQ=60)
                                       TGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGGTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGG                                                                                                               >  SRR19973069.2618276/1‑150 (MQ=60)
                                            ACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCT                                                                                                          <  SRR19973069.2891471/150‑1 (MQ=60)
                                              GGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTAC                                                                                                        <  SRR19973069.3004350/150‑1 (MQ=60)
                                              GGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTAC                                                                                                        <  SRR19973069.2622192/150‑1 (MQ=60)
                                                 GGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTGGCGATGGTGACGTCAATTCGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGCAGCTACGGT                                                                                                     >  SRR19973069.736768/1‑150 (MQ=60)
                                                  GGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATT                                                                                                    <  SRR19973069.2024874/150‑1 (MQ=60)
                                                     AAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTT                                                                                                 <  SRR19973069.3616676/150‑1 (MQ=60)
                                                     AAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTT                                                                                                 <  SRR19973069.3381148/150‑1 (MQ=60)
                                                     AAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTT                                                                                                 <  SRR19973069.2779543/150‑1 (MQ=60)
                                                     AAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTT                                                                                                 <  SRR19973069.3617327/150‑1 (MQ=60)
                                                     AAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTT                                                                                                 <  SRR19973069.3617396/150‑1 (MQ=60)
                                                      AGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGACCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTA                                                                                                <  SRR19973069.261742/150‑1 (MQ=60)
                                                       GTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTAT                                                                                               <  SRR19973069.3789717/150‑1 (MQ=60)
                                                        TTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATT                                                                                              <  SRR19973069.668220/150‑1 (MQ=60)
                                                        TTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATT                                                                                              <  SRR19973069.3803755/150‑1 (MQ=60)
                                                          CGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGT                                                                                            <  SRR19973069.780208/150‑1 (MQ=60)
                                                           GCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTT                                                                                           <  SRR19973069.2968807/150‑1 (MQ=60)
                                                           GCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTT                                                                                           <  SRR19973069.2063268/150‑1 (MQ=60)
                                                            CCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTT                                                                                          <  SRR19973069.2511299/150‑1 (MQ=60)
                                                             CAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTA                                                                                         <  SRR19973069.3683925/150‑1 (MQ=60)
                                                                 CAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATATCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGG                                                                                     >  SRR19973069.257659/1‑150 (MQ=60)
                                                                 CAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTTTTGTTTAACGG                                                                                     >  SRR19973069.2729679/1‑150 (MQ=60)
                                                                 CAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGG                                                                                     >  SRR19973069.1845721/1‑150 (MQ=60)
                                                                    GGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACC                                                                                  >  SRR19973069.1270902/1‑150 (MQ=60)
                                                                     GCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCA                                                                                 <  SRR19973069.163102/150‑1 (MQ=60)
                                                                       GTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCAGTTGAATATGCTGGTTATGTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGC                                                                               >  SRR19973069.2441487/1‑150 (MQ=60)
                                                                       GTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCGATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGC                                                                               >  SRR19973069.3623580/1‑150 (MQ=60)
                                                                         CAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAACTAATGACAACAGGAAGCTACGATTTTTATTTTTTTACGGACCAGCGT                                                                             >  SRR19973069.2971189/1‑150 (MQ=60)
                                                                         CAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTAGCATAATATCCCCCTGTTGTATATGCTGGTTATTTATACAGGTAAAATAACCTAATTACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGT                                                                             >  SRR19973069.1677560/1‑150 (MQ=60)
                                                                            TAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACC                                                                          <  SRR19973069.2769663/150‑1 (MQ=60)
                                                                             AGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCG                                                                         >  SRR19973069.612034/1‑150 (MQ=60)
                                                                               CCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTT                                                                       <  SRR19973069.711245/150‑1 (MQ=60)
                                                                                  CAGCTGGCAATGGAGAAGTTTTCGCTATTGTGACTTAAAATAGCATAAAATCCCCCAGTTGAATATGCTGGTTATTTATAAAGGTAAAATAAACAAATTACAACAGGAAGCTACGATTTTTATTGTTTTAAGGACCAGCGTACCGTTTAC                                                                    >  SRR19973069.3224736/1‑150 (MQ=60)
                                                                                  CAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAAGGACCAGCGTACCGTTTAC                                                                    >  SRR19973069.3172192/1‑150 (MQ=60)
                                                                                   AGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCC                                                                   <  SRR19973069.2303010/150‑1 (MQ=60)
                                                                                   AGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCC                                                                   <  SRR19973069.2305881/150‑1 (MQ=60)
                                                                                   AGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCC                                                                   <  SRR19973069.3234966/150‑1 (MQ=60)
                                                                                   AGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCC                                                                   <  SRR19973069.2303078/150‑1 (MQ=60)
                                                                                             GGAGAAGTTTTCGCTATGGTGACTTCAATTAGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATAAAGGTAAAATAACCTAATGACAACAGGAAGCTACCATTTTTATTTTTTAAAGGACCACCCTACCGTTTACCCGGCGCGGAA                                                         >  SRR19973069.3073627/1‑150 (MQ=60)
                                                                                             GGAGAAGTTTTCGCTATGGTGACTTCAAATCGCATAAAAGCCCCCAGTTGAATATTCTGGTTATTTATACAGGTAAAATAACCTAATGACAACACGAAGCTACCATTTTTATTGTTTAAAGGACCAGCGTACCGTTTACCCCGCGAGGCA                                                         >  SRR19973069.3074357/1‑150 (MQ=60)
                                                                                              GAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAAT                                                        <  SRR19973069.3794070/150‑1 (MQ=60)
                                                                                               AGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATG                                                       <  SRR19973069.1364062/150‑1 (MQ=60)
                                                                                               AGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATG                                                       >  SRR19973069.1641968/1‑150 (MQ=60)
                                                                                                 AAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGC                                                     <  SRR19973069.3790056/150‑1 (MQ=60)
                                                                                                 AAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGC                                                     <  SRR19973069.2244606/150‑1 (MQ=60)
                                                                                                      TTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAG                                                <  SRR19973069.612692/150‑1 (MQ=60)
                                                                                                        CGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCG                                              >  SRR19973069.3097648/1‑150 (MQ=60)
                                                                                                        CGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCG                                              >  SRR19973069.3097576/1‑150 (MQ=60)
                                                                                                           TATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGT                                           <  SRR19973069.3700637/150‑1 (MQ=60)
                                                                                                            ATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGACTGGCACCAGCGTGTC                                          >  SRR19973069.74114/1‑150 (MQ=60)
                                                                                                             TGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCA                                         <  SRR19973069.2370643/150‑1 (MQ=60)
                                                                                                              GGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATACCCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAT                                        <  SRR19973069.382487/150‑1 (MQ=60)
                                                                                                               GTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATC                                       >  SRR19973069.2186395/1‑150 (MQ=60)
                                                                                                               GTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATC                                       >  SRR19973069.2186103/1‑150 (MQ=60)
                                                                                                                   CTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTC                                   >  SRR19973069.330481/1‑150 (MQ=60)
                                                                                                                   CTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTC                                   >  SRR19973069.330445/1‑150 (MQ=60)
                                                                                                                       AATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTG                               <  SRR19973069.2499177/150‑1 (MQ=60)
                                                                                                                       AATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTG                               >  SRR19973069.276373/1‑150 (MQ=60)
                                                                                                                       AATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTG                               >  SRR19973069.163127/1‑150 (MQ=60)
                                                                                                                        ATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGC                              >  SRR19973069.2845848/1‑150 (MQ=60)
                                                                                                                         TTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCG                             <  SRR19973069.2352170/150‑1 (MQ=60)
                                                                                                                             CATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGC                         >  SRR19973069.1195535/1‑150 (MQ=60)
                                                                                                                              ATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCT                        >  SRR19973069.1400549/1‑150 (MQ=60)
                                                                                                                              ATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCT                        >  SRR19973069.833579/1‑150 (MQ=60)
                                                                                                                              ATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCT                        >  SRR19973069.528502/1‑150 (MQ=60)
                                                                                                                              ATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCT                        >  SRR19973069.833664/1‑150 (MQ=60)
                                                                                                                              ATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCT                        >  SRR19973069.1400672/1‑150 (MQ=60)
                                                                                                                                AATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTT                      >  SRR19973069.1417096/1‑150 (MQ=60)
                                                                                                                                     CCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCA                 >  SRR19973069.3396223/1‑150 (MQ=60)
                                                                                                                                        CCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACC              >  SRR19973069.241020/1‑150 (MQ=60)
                                                                                                                                         CTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCT             <  SRR19973069.3520171/150‑1 (MQ=60)
                                                                                                                                         CNGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCT             >  SRR19973069.2856501/1‑150 (MQ=60)
                                                                                                                                          TGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTG            >  SRR19973069.1638849/1‑150 (MQ=60)
                                                                                                                                          TGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTG            >  SRR19973069.2206264/1‑150 (MQ=60)
                                                                                                                                          TGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTG            >  SRR19973069.2968532/1‑150 (MQ=60)
                                                                                                                                          TGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTG            >  SRR19973069.2968497/1‑150 (MQ=60)
                                                                                                                                          TGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTG            <  SRR19973069.3528329/150‑1 (MQ=60)
                                                                                                                                               AATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCT       >  SRR19973069.1844076/1‑150 (MQ=60)
                                                                                                                                               AATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCT       <  SRR19973069.2059857/150‑1 (MQ=60)
                                                                                                                                                  ATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTT    <  SRR19973069.3272811/150‑1 (MQ=60)
                                                                                                                                                   TGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTC   <  SRR19973069.1304125/150‑1 (MQ=60)
                                                                                                                                                   TGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTC   <  SRR19973069.2432400/150‑1 (MQ=60)
                                                                                                                                                    GCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCA  >  SRR19973069.1649103/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCA  >  CP009273/1116816‑1117113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑