Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,116,964 A→G 100% intergenic (‑21/+53) dinI ← / ← pyrC DNA damage‑inducible protein I/dihydro‑orotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,116,9640AG99.2% 979.3 / ‑2.8 244intergenic (‑21/+53)dinI/pyrCDNA damage‑inducible protein I/dihydro‑orotase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (125/117);  total (126/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

GTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCT  >  CP009273/1116817‑1117110
                                                                                                                                                   |                                                                                                                                                  
gTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCt                                                                                                                                                  >  1:2687162/1‑150 (MQ=255)
gTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCt                                                                                                                                                  >  2:2597975/1‑150 (MQ=255)
 tACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGTATATGCTg                                                                                                                                                 >  1:523029/1‑150 (MQ=255)
 tACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTg                                                                                                                                                 <  1:3113570/150‑1 (MQ=255)
  aCCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTgg                                                                                                                                                <  1:3820389/150‑1 (MQ=255)
      aTACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGttat                                                                                                                                            >  1:1022455/1‑150 (MQ=255)
       tACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGttatt                                                                                                                                           <  1:3197875/150‑1 (MQ=255)
        aCGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGttattt                                                                                                                                          <  2:3405713/150‑1 (MQ=255)
             gCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACa                                                                                                                                     >  2:829547/1‑150 (MQ=255)
              ccTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAg                                                                                                                                    >  1:3689512/1‑150 (MQ=255)
              ccTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAg                                                                                                                                    <  2:2807875/150‑1 (MQ=255)
              ccTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAg                                                                                                                                    >  2:427215/1‑150 (MQ=255)
               cTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAgg                                                                                                                                   >  2:3676266/1‑150 (MQ=255)
                ttCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGt                                                                                                                                  <  2:2327397/150‑1 (MQ=255)
                  cATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTaa                                                                                                                                >  1:4118483/1‑150 (MQ=255)
                     tATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTCACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAAt                                                                                                                             <  2:3842428/150‑1 (MQ=255)
                       tCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATaa                                                                                                                           <  2:2221360/150‑1 (MQ=255)
                         aGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAcc                                                                                                                         <  2:2599271/150‑1 (MQ=255)
                         aGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAcc                                                                                                                         <  2:3491164/150‑1 (MQ=255)
                           gAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTa                                                                                                                       <  1:211649/150‑1 (MQ=255)
                             aaCGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAAt                                                                                                                     <  1:2630095/150‑1 (MQ=255)
                             aaCGCATACTGAATACGGAGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAAt                                                                                                                     <  1:122471/150‑1 (MQ=255)
                                   tACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGacaac                                                                                                               <  1:1013296/150‑1 (MQ=255)
                                    aCTGAATACGGTGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTAGCTATGGTGACTTCAATTCTCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGacaaca                                                                                                              <  1:64763/150‑1 (MQ=255)
                                          tACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGACGc                                                                                                        >  2:1641650/1‑150 (MQ=255)
                                              gcggGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACg                                                                                                    <  1:2188890/150‑1 (MQ=255)
                                               cggGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGa                                                                                                   >  2:3176226/1‑150 (MQ=255)
                                                 ggAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAtt                                                                                                 <  2:1505316/150‑1 (MQ=255)
                                                 ggAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAtt                                                                                                 >  1:3473509/1‑150 (MQ=255)
                                                  gAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAttt                                                                                                <  2:2182260/150‑1 (MQ=255)
                                                   aaaGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAtttt                                                                                               <  2:3151977/150‑1 (MQ=255)
                                                    aaGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAttttt                                                                                              <  1:2366665/150‑1 (MQ=255)
                                                      gTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTAt                                                                                            <  1:717131/150‑1 (MQ=255)
                                                        tCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTg                                                                                          <  2:3821912/150‑1 (MQ=255)
                                                        tCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTg                                                                                          >  1:3250644/1‑150 (MQ=255)
                                                        tCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTg                                                                                          >  1:2223606/1‑150 (MQ=255)
                                                          gccagccaGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGtt                                                                                        >  2:2023914/1‑150 (MQ=255)
                                                          gccagccaGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGtt                                                                                        >  1:3451095/1‑150 (MQ=255)
                                                          gccagccaGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGtt                                                                                        >  2:4124499/1‑150 (MQ=255)
                                                          gccagccaGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGtt                                                                                        >  1:1357941/1‑150 (MQ=255)
                                                            cagccagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTa                                                                                      <  2:4028360/150‑1 (MQ=255)
                                                              gccagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAc                                                                                    >  2:3224351/1‑150 (MQ=255)
                                                              gccagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGCTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTacc                                                                                    <  1:3765431/150‑3 (MQ=255)
                                                               ccagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACg                                                                                   <  2:1550493/150‑1 (MQ=255)
                                                               ccagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACg                                                                                   <  2:1550400/150‑1 (MQ=255)
                                                               ccagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACg                                                                                   >  1:829972/1‑150 (MQ=255)
                                                                cagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACgg                                                                                  <  2:1265187/150‑1 (MQ=255)
                                                                 agGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGa                                                                                 >  2:407493/1‑150 (MQ=255)
                                                                 agGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGa                                                                                 >  1:2051158/1‑150 (MQ=255)
                                                                  gggCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGAc                                                                                >  2:3641975/1‑150 (MQ=255)
                                                                      gTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGc                                                                            >  1:2829136/1‑150 (MQ=255)
                                                                      gTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGc                                                                            <  1:1907865/150‑1 (MQ=255)
                                                                       tCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCg                                                                           >  2:3932669/1‑150 (MQ=255)
                                                                        cAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGt                                                                          >  1:693559/1‑150 (MQ=255)
                                                                        cAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGt                                                                          >  2:1761031/1‑150 (MQ=255)
                                                                           tAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTAcc                                                                       <  1:234224/150‑1 (MQ=255)
                                                                            aGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTAAATTCGCATAAAATCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAACTAATTACAACAGGAAGCTAAGATTTTTATTGTTTAACGGACCAACGTACCg                                                                      >  1:3281098/1‑150 (MQ=255)
                                                                              ccccAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGtt                                                                    <  1:3673524/150‑1 (MQ=255)
                                                                                    cTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCg                                                              >  1:1665967/1‑150 (MQ=255)
                                                                                         aaTGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAg                                                         <  1:1204905/150‑1 (MQ=255)
                                                                                          aTGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAgg                                                        <  2:4093006/150‑1 (MQ=255)
                                                                                           tGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGa                                                       >  2:3724875/1‑150 (MQ=255)
                                                                                            ggAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGaa                                                      >  2:890836/1‑150 (MQ=255)
                                                                                             gagaAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAAt                                                     <  2:1665153/150‑1 (MQ=255)
                                                                                             gagaAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAAt                                                     <  1:1123938/150‑1 (MQ=255)
                                                                                             gagaAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAAt                                                     <  1:1124092/150‑1 (MQ=255)
                                                                                             gagaAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAAt                                                     >  1:1714794/1‑150 (MQ=255)
                                                                                                 aGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCa                                                 >  2:3149128/1‑150 (MQ=255)
                                                                                                    tttCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCa                                              <  1:1076731/150‑1 (MQ=255)
                                                                                                    tttCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCa                                              <  1:294808/150‑1 (MQ=255)
                                                                                                       cGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCg                                           <  1:54051/150‑1 (MQ=255)
                                                                                                         cTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCgtg                                         <  2:2089638/150‑1 (MQ=255)
                                                                                                          tATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCgtgt                                        >  1:898583/1‑150 (MQ=255)
                                                                                                               tGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGtcatca                                   >  1:2329188/1‑150 (MQ=255)
                                                                                                               tGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGtcatca                                   <  2:2912425/150‑1 (MQ=255)
                                                                                                                gACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAACTAACCTACTGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAtcag                                  <  2:2841424/150‑1 (MQ=255)
                                                                                                                  cTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAtcagtc                                >  2:1073933/1‑150 (MQ=255)
                                                                                                                   ttCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAtcagtca                               >  1:500434/1‑150 (MQ=255)
                                                                                                                   ttCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAtcagtca                               <  1:4095728/150‑1 (MQ=255)
                                                                                                                   ttCAAGTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAtcagtca                               <  2:2280979/150‑1 (MQ=255)
                                                                                                                    tCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAtcagtcag                              >  2:247799/1‑150 (MQ=255)
                                                                                                                       aTTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGc                           <  2:2734352/150‑1 (MQ=255)
                                                                                                                        ttCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCg                          <  1:448865/150‑1 (MQ=255)
                                                                                                                         tCGCATAATAGCCCGATGTTGAATATGCTGTTTATTTATACAGGTAAAATATCCTAATGACAACAGGAAGATACGATTTTTATTGTTTATCGGACCAGCGTTCCGTTTCCCCGGCGAGGAATGGCACCTGCGTGTCATCAGTCAGTGcta                         <  1:2800122/150‑3 (MQ=255)
                                                                                                                          cGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGAt                        >  2:1122658/1‑150 (MQ=255)
                                                                                                                           gCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATg                       >  1:1535999/1‑150 (MQ=255)
                                                                                                                             atactaGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCt                     <  2:720938/150‑1 (MQ=255)
                                                                                                                             ataataGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCt                     >  2:2935943/1‑150 (MQ=255)
                                                                                                                              taataGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCtt                    >  2:17263/1‑150 (MQ=255)
                                                                                                                                ataGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTc                  >  2:3072070/1‑150 (MQ=255)
                                                                                                                                ataGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTc                  >  2:3072042/1‑150 (MQ=255)
                                                                                                                                    cccccTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCa              >  1:3609477/1‑150 (MQ=255)
                                                                                                                                     ccccTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCaa             >  2:660363/1‑150 (MQ=255)
                                                                                                                                          gTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGt        >  1:3500761/1‑150 (MQ=255)
                                                                                                                                            tGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTg      <  1:2597979/150‑1 (MQ=255)
                                                                                                                                            tGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTg      >  1:218589/1‑150 (MQ=255)
                                                                                                                                              aaTATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGct    >  1:605982/1‑150 (MQ=255)
                                                                                                                                                tatgCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGctct  >  1:2452049/1‑150 (MQ=255)
                                                                                                                                                tatgCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGctct  <  2:1397836/150‑1 (MQ=255)
                                                                                                                                                tatgCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGctct  <  2:3492661/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCT  >  CP009273/1116817‑1117110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCA  >  CP009273/1116817‑1117113
                                                                                                                                                   |                                                                                                                                                     
GTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCT                                                                                                                                                     >  SRR19973074.2692154/1‑150 (MQ=60)
 TACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGTATATGCTG                                                                                                                                                    >  SRR19973074.524071/1‑150 (MQ=60)
 TACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTG                                                                                                                                                    <  SRR19973074.3119281/150‑1 (MQ=60)
  ACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGG                                                                                                                                                   <  SRR19973074.3827417/150‑1 (MQ=60)
  ACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGG                                                                                                                                                   <  SRR19973074.3827361/150‑1 (MQ=60)
   CCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGT                                                                                                                                                  <  SRR19973074.341478/150‑1 (MQ=60)
     GATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTA                                                                                                                                                >  SRR19973074.2945097/1‑150 (MQ=60)
      ATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTAT                                                                                                                                               >  SRR19973074.1024349/1‑150 (MQ=60)
       TACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATT                                                                                                                                              <  SRR19973074.3203770/150‑1 (MQ=60)
       TACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATT                                                                                                                                              <  SRR19973074.3203701/150‑1 (MQ=60)
           TGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATA                                                                                                                                          <  SRR19973074.1940828/150‑1 (MQ=60)
             GCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACA                                                                                                                                        <  SRR19973074.1341747/150‑1 (MQ=60)
              CCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAG                                                                                                                                       >  SRR19973074.3696335/1‑150 (MQ=60)
              CCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATACAG                                                                                                                                       >  SRR19973074.3695943/1‑150 (MQ=60)
                 TCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTA                                                                                                                                    <  SRR19973074.1220646/150‑1 (MQ=60)
                  CATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAA                                                                                                                                   >  SRR19973074.4126081/1‑150 (MQ=60)
                     TATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAAT                                                                                                                                <  SRR19973074.985018/150‑1 (MQ=60)
                     TATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAAT                                                                                                                                <  SRR19973074.258683/150‑1 (MQ=60)
                         AGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACC                                                                                                                            >  SRR19973074.4019895/1‑150 (MQ=60)
                           GAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTA                                                                                                                          <  SRR19973074.212089/150‑1 (MQ=60)
                             AACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAAT                                                                                                                        <  SRR19973074.2635013/150‑1 (MQ=60)
                             AACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAAT                                                                                                                        <  SRR19973074.2107213/150‑1 (MQ=60)
                             AACGCATACTGAATACGGAGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAAT                                                                                                                        <  SRR19973074.122756/150‑1 (MQ=60)
                                GCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGAC                                                                                                                     <  SRR19973074.3341507/150‑1 (MQ=60)
                                  ATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGATATGGTGACTTCAATTAGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGAAAAAAAACCTAAGGACAA                                                                                                                   >  SRR19973074.566223/1‑150 (MQ=60)
                                   TACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAAC                                                                                                                  <  SRR19973074.1015177/150‑1 (MQ=60)
                                    ACTGAATCCGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACA                                                                                                                 <  SRR19973074.64928/150‑1 (MQ=60)
                                    ACTGAATACGGTGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTAGCTATGGTGACTTCAATTCTCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACA                                                                                                                 <  SRR19973074.64931/150‑1 (MQ=60)
                                      TGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGG                                                                                                               <  SRR19973074.2240403/150‑1 (MQ=60)
                                        AATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAA                                                                                                             <  SRR19973074.308150/150‑1 (MQ=60)
                                        AATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAA                                                                                                             >  SRR19973074.1126053/1‑150 (MQ=60)
                                        AATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAA                                                                                                             >  SRR19973074.1126173/1‑150 (MQ=60)
                                         ATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAG                                                                                                            <  SRR19973074.3699201/150‑1 (MQ=60)
                                          TACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGTCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGC                                                                                                           <  SRR19973074.2195973/150‑1 (MQ=60)
                                          TACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGC                                                                                                           >  SRR19973074.514256/1‑150 (MQ=60)
                                            CGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTA                                                                                                         <  SRR19973074.52112/150‑1 (MQ=60)
                                              GCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACG                                                                                                       <  SRR19973074.2192997/150‑1 (MQ=60)
                                                GGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAT                                                                                                     >  SRR19973074.1553203/1‑150 (MQ=60)
                                                GGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAT                                                                                                     <  SRR19973074.2829138/150‑1 (MQ=60)
                                                 GGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATT                                                                                                    >  SRR19973074.3479891/1‑150 (MQ=60)
                                                    AAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTT                                                                                                 <  SRR19973074.572532/150‑1 (MQ=60)
                                                    AAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTT                                                                                                 >  SRR19973074.764871/1‑150 (MQ=60)
                                                    AAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTT                                                                                                 <  SRR19973074.2371068/150‑1 (MQ=60)
                                                    AAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTT                                                                                                 <  SRR19973074.2371031/150‑1 (MQ=60)
                                                    AAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTT                                                                                                 <  SRR19973074.2371048/150‑1 (MQ=60)
                                                      GTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTAT                                                                                               <  SRR19973074.718471/150‑1 (MQ=60)
                                                      GTTCGCCAGCCAGGGCGTCAAGAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTAT                                                                                               <  SRR19973074.718455/150‑1 (MQ=60)
                                                        TCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTG                                                                                             >  SRR19973074.3256661/1‑150 (MQ=60)
                                                        TCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTG                                                                                             >  SRR19973074.2227793/1‑150 (MQ=60)
                                                          GCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTT                                                                                           <  SRR19973074.1104855/150‑1 (MQ=60)
                                                          GCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTT                                                                                           >  SRR19973074.1360462/1‑150 (MQ=60)
                                                          GCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTT                                                                                           >  SRR19973074.3457442/1‑150 (MQ=60)
                                                          GCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTT                                                                                           >  SRR19973074.3457456/1‑150 (MQ=60)
                                                            CAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATTACAACAGGAAGCTACGATTTTTATTTTTTA                                                                                         >  SRR19973074.818056/1‑150 (MQ=60)
                                                            CAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTA                                                                                         <  SRR19973074.2770467/150‑1 (MQ=60)
                                                             AGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAA                                                                                        <  SRR19973074.3269554/150‑1 (MQ=60)
                                                              GCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC                                                                                       <  SRR19973074.3772660/150‑1 (MQ=60)
                                                              GCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGCTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTACC                                                                                       <  SRR19973074.3772347/150‑1 (MQ=60)
                                                               CCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACG                                                                                      >  SRR19973074.831568/1‑150 (MQ=60)
                                                               CCAGGGCGTCAACAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACG                                                                                      >  SRR19973074.831576/1‑150 (MQ=60)
                                                                 AGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGA                                                                                    >  SRR19973074.2055017/1‑150 (MQ=60)
                                                                     CGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAG                                                                                <  SRR19973074.2170061/150‑1 (MQ=60)
                                                                      GTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGC                                                                               <  SRR19973074.1911525/150‑1 (MQ=60)
                                                                      GTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGC                                                                               <  SRR19973074.2298540/150‑1 (MQ=60)
                                                                      GTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGC                                                                               >  SRR19973074.2834300/1‑150 (MQ=60)
                                                                      GTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGC                                                                               >  SRR19973074.2834370/1‑150 (MQ=60)
                                                                        CAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGT                                                                             >  SRR19973074.694834/1‑150 (MQ=60)
                                                                           TAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACC                                                                          <  SRR19973074.234661/150‑1 (MQ=60)
                                                                           TAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACC                                                                          <  SRR19973074.234702/150‑1 (MQ=60)
                                                                           TAGCCCCAGCTGGCAATGGAGAAGTTTTAGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACC                                                                          >  SRR19973074.360024/1‑150 (MQ=60)
                                                                            AGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTAAATTCGCATAAAATCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAACTAATTACAACAGGAAGCTAAGATTTTTATTGTTTAACGGACCAACGTACCG                                                                         >  SRR19973074.3287187/1‑150 (MQ=60)
                                                                              CCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTT                                                                       <  SRR19973074.3680318/150‑1 (MQ=60)
                                                                               CCCAGCTGGCAATGGAGAAGTTTTCGCTATTGTGACTTCCATTCGCATAAAAGCCCCCAGTTGAATATGCATGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCGACGATTTTTATTTTTTAACGGACCAGCGTACCGTTT                                                                      >  SRR19973074.2650099/1‑150 (MQ=60)
                                                                                 CAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCC                                                                    >  SRR19973074.1464033/1‑150 (MQ=60)
                                                                                  AGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCC                                                                   >  SRR19973074.1030956/1‑150 (MQ=60)
                                                                                    CTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCG                                                                 >  SRR19973074.1669108/1‑150 (MQ=60)
                                                                                     TGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGG                                                                >  SRR19973074.1699571/1‑150 (MQ=60)
                                                                                         AATGGAGAAGTTTTCGCTATGGTGACTTCAATTCTCATAATAGCCCCCTGTTGTATATGCTGGTTATTTATACTGGTATAATAACCTAATGACATCAGGAAGCTACGATTTTTATTGTTTATAGGACCAGCGTAACGTTTCCCCGGCGAG                                                            <  SRR19973074.348684/150‑1 (MQ=60)
                                                                                         AATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAG                                                            <  SRR19973074.1207213/150‑1 (MQ=60)
                                                                                             GAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAAT                                                        <  SRR19973074.1126216/150‑1 (MQ=60)
                                                                                             GAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAAT                                                        >  SRR19973074.1717982/1‑150 (MQ=60)
                                                                                             GAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAAT                                                        <  SRR19973074.1126061/150‑1 (MQ=60)
                                                                                                 AGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCA                                                    <  SRR19973074.3237392/150‑1 (MQ=60)
                                                                                                    TTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCA                                                 <  SRR19973074.1078739/150‑1 (MQ=60)
                                                                                                    TTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCA                                                 <  SRR19973074.2439446/150‑1 (MQ=60)
                                                                                                    TTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCA                                                 <  SRR19973074.295356/150‑1 (MQ=60)
                                                                                                    TTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCA                                                 <  SRR19973074.3498319/150‑1 (MQ=60)
                                                                                                       CGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCG                                              <  SRR19973074.54196/150‑1 (MQ=60)
                                                                                                          TATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGT                                           >  SRR19973074.900295/1‑150 (MQ=60)
                                                                                                           ATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTC                                          >  SRR19973074.3235079/1‑150 (MQ=60)
                                                                                                             GGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCCACAGCGTGTCAT                                        >  SRR19973074.3080255/1‑150 (MQ=60)
                                                                                                               TGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCA                                      >  SRR19973074.209234/1‑150 (MQ=60)
                                                                                                               TGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCA                                      >  SRR19973074.2333533/1‑150 (MQ=60)
                                                                                                               TGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCA                                      <  SRR19973074.4104471/150‑1 (MQ=60)
                                                                                                                GACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAG                                     >  SRR19973074.1145589/1‑150 (MQ=60)
                                                                                                                 ACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGT                                    >  SRR19973074.1322810/1‑150 (MQ=60)
                                                                                                                   TTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCA                                  <  SRR19973074.4103294/150‑1 (MQ=60)
                                                                                                                   TTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCA                                  >  SRR19973074.501437/1‑150 (MQ=60)
                                                                                                                       ATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGC                              >  SRR19973074.461808/1‑150 (MQ=60)
                                                                                                                        TTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCG                             <  SRR19973074.1653471/150‑1 (MQ=60)
                                                                                                                        TTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCG                             >  SRR19973074.1198651/1‑150 (MQ=60)
                                                                                                                        TTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCG                             <  SRR19973074.428716/150‑1 (MQ=60)
                                                                                                                        TTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCG                             <  SRR19973074.449736/150‑1 (MQ=60)
                                                                                                                         TCGCATAATAGCCCGATGTTGAATATGCTGTTTATTTATACAGGTAAAATATCCTAATGACAACAGGAAGATACGATTTTTATTGTTTATCGGACCAGCGTTCCGTTTCCCCGGCGAGGAATGGCACCTGCGTGTCATCAGTCAGTGCTA                            <  SRR19973074.2805287/150‑1 (MQ=60)
                                                                                                                          CGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGAT                           <  SRR19973074.67478/150‑1 (MQ=60)
                                                                                                                           GCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATG                          >  SRR19973074.1538915/1‑150 (MQ=60)
                                                                                                                                  AGCCCACTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAG                   <  SRR19973074.3677102/150‑1 (MQ=60)
                                                                                                                                    CCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCA                 >  SRR19973074.3616145/1‑150 (MQ=60)
                                                                                                                                    CCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCA                 >  SRR19973074.3616448/1‑150 (MQ=60)
                                                                                                                                         TGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTG            <  SRR19973074.2672416/150‑1 (MQ=60)
                                                                                                                                          GTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGT           >  SRR19973074.1022229/1‑150 (MQ=60)
                                                                                                                                          GTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGT           >  SRR19973074.3507192/1‑150 (MQ=60)
                                                                                                                                           TTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTT          <  SRR19973074.440777/150‑1 (MQ=60)
                                                                                                                                            TGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCggcgacccgttgccccgcgtaggaatgccaccagggttaaaacatgaggtcctatgattaaagaaccatgttg         >  SRR19973074.4000720/1‑77 (MQ=60)
                                                                                                                                            TGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTG         >  SRR19973074.3735964/1‑150 (MQ=60)
                                                                                                                                            TGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTG         >  SRR19973074.219041/1‑150 (MQ=60)
                                                                                                                                            TGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTG         <  SRR19973074.2602935/150‑1 (MQ=60)
                                                                                                                                            TGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTG         <  SRR19973074.2602836/150‑1 (MQ=60)
                                                                                                                                            TGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTG         <  SRR19973074.2602832/150‑1 (MQ=60)
                                                                                                                                            TGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTG         >  SRR19973074.219043/1‑150 (MQ=60)
                                                                                                                                            TGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTG         >  SRR19973074.527105/1‑150 (MQ=60)
                                                                                                                                            TGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTG         >  SRR19973074.219310/1‑150 (MQ=60)
                                                                                                                                              AATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCT       >  SRR19973074.607135/1‑150 (MQ=60)
                                                                                                                                              AATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCT       >  SRR19973074.1875420/1‑150 (MQ=60)
                                                                                                                                              AATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCT       >  SRR19973074.1875526/1‑150 (MQ=60)
                                                                                                                                                TATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGACTGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCT     >  SRR19973074.656181/1‑150 (MQ=60)
                                                                                                                                                TATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCT     >  SRR19973074.2456584/1‑150 (MQ=60)
                                                                                                                                                   GCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCA  >  SRR19973074.1545873/1‑150 (MQ=60)
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GTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCA  >  CP009273/1116817‑1117113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑