Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F56 I0 R1 543 285.0 9366284 95.7% 8963533 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,116,964 A→G 81.5% intergenic (‑21/+53) dinI ← / ← pyrC DNA damage‑inducible protein I/dihydro‑orotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,116,9640AG81.5% 591.1 / 117.7 238intergenic (‑21/+53)dinI/pyrCDNA damage‑inducible protein I/dihydro‑orotase
Reads supporting (aligned to +/- strand):  ref base A (20/24);  new base G (86/107);  total (107/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01

Only 100 of 242 total aligned reads displayed.

TACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTC  >  CP009273/1116818‑1117112
                                                                                                                                                  |                                                                                                                                                    
tACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCt                                                                                                                                                    <  2:3989083/149‑1 (MQ=255)
  ccGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTgg                                                                                                                                                  >  2:883704/1‑149 (MQ=255)
   cGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGt                                                                                                                                                 <  2:3428608/149‑1 (MQ=255)
   cGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGt                                                                                                                                                 <  2:2787768/149‑1 (MQ=255)
    gATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAGGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGtt                                                                                                                                                <  2:1320369/149‑1 (MQ=255)
      tACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGttat                                                                                                                                              <  1:4263074/149‑1 (MQ=255)
      tACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGttat                                                                                                                                              <  1:2300881/149‑1 (MQ=255)
        cGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGttattt                                                                                                                                            <  2:3460403/149‑1 (MQ=255)
         gTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGttattta                                                                                                                                           >  2:4506779/1‑149 (MQ=255)
         gTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGttattta                                                                                                                                           <  1:2833197/149‑1 (MQ=255)
         gTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGttattta                                                                                                                                           >  1:889166/1‑149 (MQ=255)
          tGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGGTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGttatttat                                                                                                                                          <  2:3266982/149‑1 (MQ=255)
            gCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATAc                                                                                                                                        >  2:2155697/1‑149 (MQ=255)
            gCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATAc                                                                                                                                        <  1:3847806/149‑1 (MQ=255)
              cTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAg                                                                                                                                      >  2:930100/1‑149 (MQ=255)
               ttCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAgg                                                                                                                                     <  1:4153332/149‑1 (MQ=255)
                 cATTATCAGGAAACGCATACTGACGACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTa                                                                                                                                   <  2:1446770/149‑1 (MQ=255)
                    tATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTaaaa                                                                                                                                >  1:575765/1‑149 (MQ=255)
                    tATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCGAAGGTGACTTCAATTCGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATACAGGTaaaa                                                                                                                                >  1:2774157/1‑149 (MQ=255)
                     aTCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGGTGAATATGCTGGTTATTTATACAGGTAAAAt                                                                                                                               >  1:2864668/1‑149 (MQ=255)
                       cAGGAAACGCAGACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATaa                                                                                                                             <  2:4316084/149‑1 (MQ=255)
                         ggAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAcc                                                                                                                           <  1:3256039/149‑1 (MQ=255)
                           aaaCGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTGTTCGCTATGGTGACATAAATTCGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATACAGGGAAAATAAccga                                                                                                                         >  1:1572037/1‑147 (MQ=255)
                            aaCGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTAGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTaa                                                                                                                        >  1:263984/1‑149 (MQ=255)
                                cATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGac                                                                                                                    <  1:3215317/149‑1 (MQ=255)
                                             gcggGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTAc                                                                                                       <  1:466349/149‑1 (MQ=255)
                                                 gAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAtt                                                                                                   <  1:2102528/149‑1 (MQ=255)
                                                 gAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAtt                                                                                                   <  2:3751583/149‑1 (MQ=255)
                                                 gAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTAGCGATGGTGACTTCAATTCGCATAATAGCCCCCAGGTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCGACGAtt                                                                                                   >  1:3562247/1‑149 (MQ=255)
                                                    aGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAttttt                                                                                                >  2:3495409/1‑149 (MQ=255)
                                                      ttCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTAt                                                                                              <  1:2753679/149‑1 (MQ=255)
                                                         gccagccaGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAAACTAATGACAACAGGAAGCTACGATTTTTATTGt                                                                                           >  1:1751487/1‑149 (MQ=255)
                                                          ccagccagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGtt                                                                                          <  2:3017610/149‑1 (MQ=255)
                                                          ccagccagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTAGCTATGGTGACTTCAATTCGCATAATATCCCCCAGTTGAATATGCTGGTTATTTATACAGGTAAAAAAACCTAATGACAACAGGAAGCTACGatttttattttt                                                                                          >  1:4335149/1‑149 (MQ=255)
                                                           cagccagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGttt                                                                                         >  1:4489260/1‑149 (MQ=255)
                                                            agccagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTa                                                                                        <  1:1604794/149‑1 (MQ=255)
                                                             gccagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTaa                                                                                       >  2:3652799/1‑149 (MQ=255)
                                                              ccagGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAc                                                                                      >  1:4360250/1‑149 (MQ=255)
                                                                agGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACgg                                                                                    >  2:177154/1‑149 (MQ=255)
                                                                 gggCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAACTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAAGGa                                                                                   >  1:781711/1‑149 (MQ=255)
                                                                  ggCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGAc                                                                                  <  2:270113/149‑1 (MQ=255)
                                                                  ggCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGAc                                                                                  <  2:269874/149‑1 (MQ=255)
                                                                    cGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCa                                                                                <  2:1961077/149‑1 (MQ=255)
                                                                    cGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCa                                                                                <  1:1999340/149‑1 (MQ=255)
                                                                           aGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTAGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTAcc                                                                         >  1:1002047/1‑149 (MQ=255)
                                                                           aGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTAGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCAAATGACAACAGGAAGCTAAGATTTTTATTGTTTAACGGACCAGCGTAcc                                                                         >  1:2831939/1‑149 (MQ=255)
                                                                           aGCCCCAGCTGGCAATGGAGAAGTTTTCGCGATGGTGACTTCAATTCGCATAATAGCCCCCAGTTGAATATGCAGGTTATTTAAACAGGTAAAATAACCTAATGACAACAGGAAGCAACGATTTTTAATTTTTAACGGACCAGCGTAcc                                                                         >  2:1703230/1‑149 (MQ=255)
                                                                             ccccAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGt                                                                       <  1:3635214/149‑1 (MQ=255)
                                                                              cccAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGtt                                                                      >  2:4463675/1‑149 (MQ=255)
                                                                                cAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTc                                                                    <  2:547879/149‑1 (MQ=255)
                                                                                cAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTc                                                                    <  2:2094821/149‑1 (MQ=255)
                                                                                 aGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTATAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTcc                                                                   <  1:3954925/149‑1 (MQ=255)
                                                                                   cTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTcccc                                                                 <  1:4063487/149‑1 (MQ=255)
                                                                                      gCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATATCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGc                                                              >  2:1622211/1‑149 (MQ=255)
                                                                                      gCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGc                                                              >  2:744340/1‑149 (MQ=255)
                                                                                       cAATGGAGAAGTTTTCGCTATGGTGACTTCAATTAGCATAATATCCCCCTGTTGAATATGCTGGTTATTTATAAAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTTTTTAACGGACCAGCGTACCGTTTCCCCGGCg                                                             >  1:958739/1‑149 (MQ=255)
                                                                                        aaTGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGa                                                            <  2:2205423/149‑1 (MQ=255)
                                                                                         aTGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAg                                                           >  2:3835688/1‑149 (MQ=255)
                                                                                            gagaAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGaa                                                        <  2:2761680/149‑1 (MQ=255)
                                                                                             agaAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAAt                                                       <  2:4335149/149‑1 (MQ=255)
                                                                                             agaAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAAt                                                       <  1:3110138/149‑1 (MQ=255)
                                                                                             agaAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAAt                                                       <  1:4457158/149‑1 (MQ=255)
                                                                                                      cGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGc                                              <  2:1959053/149‑1 (MQ=255)
                                                                                                      cGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGc                                              <  2:2554490/149‑1 (MQ=255)
                                                                                                       gCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCg                                             >  2:3207359/1‑149 (MQ=255)
                                                                                                          aTGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCgtgt                                          <  1:1468406/149‑1 (MQ=255)
                                                                                                               gACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGtcatca                                     >  1:2459801/1‑149 (MQ=255)
                                                                                                                   tCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAtcagtca                                 <  2:2374251/149‑1 (MQ=255)
                                                                                                                    cAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAtcagtcag                                <  2:2480680/149‑1 (MQ=255)
                                                                                                                    cAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAtcagtcag                                <  2:3128398/149‑1 (MQ=255)
                                                                                                                     aaTTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATcagtcagt                               <  2:2614901/149‑1 (MQ=255)
                                                                                                                     aaTTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATcagtcagt                               >  2:4603142/1‑149 (MQ=255)
                                                                                                                      aTTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTg                              >  1:2144181/1‑149 (MQ=255)
                                                                                                                      aTTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTg                              >  1:1400827/1‑149 (MQ=255)
                                                                                                                       ttCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGc                             <  2:575765/149‑1 (MQ=255)
                                                                                                                         cGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGa                           <  2:201446/149‑1 (MQ=255)
                                                                                                                         cGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGa                           <  1:4116343/149‑1 (MQ=255)
                                                                                                                         cGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGa                           <  1:3114944/149‑1 (MQ=255)
                                                                                                                          gCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGAt                          <  1:4237090/149‑1 (MQ=255)
                                                                                                                           cATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATg                         >  2:1642037/1‑149 (MQ=255)
                                                                                                                             taataGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCt                       >  1:339589/1‑149 (MQ=255)
                                                                                                                             taataGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCt                       <  2:4044226/149‑1 (MQ=255)
                                                                                                                               ataGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCttt                     >  2:4479247/1‑149 (MQ=255)
                                                                                                                                taGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTc                    >  2:130980/1‑149 (MQ=255)
                                                                                                                                taGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTc                    <  1:2551552/149‑1 (MQ=255)
                                                                                                                                  gCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAg                  >  1:3693675/1‑149 (MQ=255)
                                                                                                                                  gCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAg                  >  1:1788332/1‑149 (MQ=255)
                                                                                                                                    ccccTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCa                >  1:3328223/1‑149 (MQ=255)
                                                                                                                                      ccTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGCAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTGTCCCCGGCGAGGACTGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAAc              >  2:684926/1‑149 (MQ=255)
                                                                                                                                      ccTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAAc              >  2:3325457/1‑149 (MQ=255)
                                                                                                                                      ccTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAAc              >  1:3545814/1‑149 (MQ=255)
                                                                                                                                        tGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCt            >  2:671343/1‑149 (MQ=255)
                                                                                                                                        tGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTCCGATTTTGATAGAATAACGGACCAGCGAACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCt            >  1:2989361/1‑149 (MQ=255)
                                                                                                                                         gTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGACTGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTg           >  1:4089095/1‑149 (MQ=255)
                                                                                                                                         gTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTg           <  2:4078758/149‑1 (MQ=255)
                                                                                                                                         gTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTg           >  1:1364156/1‑149 (MQ=255)
                                                                                                                                           tGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGtt         <  2:4156011/149‑1 (MQ=255)
                                                                                                                                             aaTATGCTGGTTATTTATACAGGTATAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGc       <  1:534105/149‑1 (MQ=255)
                                                                                                                                             aaTATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGc       >  2:1695085/1‑149 (MQ=255)
                                                                                                                                               tataCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGctc     <  2:124985/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTC  >  CP009273/1116818‑1117112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCA  >  CP009273/1116817‑1117113
                                                                                                                                                   |                                                                                                                                                     
GTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCT                                                                                                                                                     <  SRR19973085.3456269/150‑1 (MQ=60)
GTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCT                                                                                                                                                     <  SRR19973085.1318999/150‑1 (MQ=60)
 TACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTG                                                                                                                                                    <  SRR19973085.3701854/150‑1 (MQ=60)
  ACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGG                                                                                                                                                   >  SRR19973085.2678854/1‑150 (MQ=60)
       TACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATT                                                                                                                                              <  SRR19973085.2310745/150‑1 (MQ=60)
       TACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATT                                                                                                                                              <  SRR19973085.4280644/150‑1 (MQ=60)
         CGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTA                                                                                                                                            >  SRR19973085.893129/1‑150 (MQ=60)
          GTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTAT                                                                                                                                           <  SRR19973085.2845008/150‑1 (MQ=60)
          GTGGCCTTCATTATCAGGAAACGCAGACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTAT                                                                                                                                           <  SRR19973085.2845233/150‑1 (MQ=60)
             GCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACA                                                                                                                                        <  SRR19973085.3863731/150‑1 (MQ=60)
               CTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGTATATTCTGGTTATTTATAAAGG                                                                                                                                      >  SRR19973085.4569054/1‑150 (MQ=60)
               CTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGG                                                                                                                                      <  SRR19973085.949277/150‑1 (MQ=60)
                TTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGT                                                                                                                                     <  SRR19973085.4170637/150‑1 (MQ=60)
                  CATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAA                                                                                                                                   <  SRR19973085.356313/150‑1 (MQ=60)
                    TTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTTAAA                                                                                                                                 >  SRR19973085.578429/1‑150 (MQ=60)
                    TTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCGAAGGTGACTTCAATTCGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATACACGTAAAA                                                                                                                                 >  SRR19973085.2785857/1‑150 (MQ=60)
                     TATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAAT                                                                                                                                <  SRR19973085.4128486/150‑1 (MQ=60)
                     TATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGGTGAATATGCTGGTTATTTATACAGGTAAAAT                                                                                                                                >  SRR19973085.2876865/1‑150 (MQ=60)
                        CAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAC                                                                                                                             <  SRR19973085.2812594/150‑1 (MQ=60)
                        CAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAC                                                                                                                             <  SRR19973085.3945485/150‑1 (MQ=60)
                          GGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCT                                                                                                                           <  SRR19973085.1331852/150‑1 (MQ=60)
                          GGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCT                                                                                                                           <  SRR19973085.3269731/150‑1 (MQ=60)
                          GGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCT                                                                                                                           <  SRR19973085.4094196/150‑1 (MQ=60)
                           GAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTA                                                                                                                          >  SRR19973085.2886689/1‑150 (MQ=60)
                           GAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGGGACTTAAAATCGCATAATATCCCCCAGTTGAATATGCTGGTTATTTATACAGGTAAAAAAACCAA                                                                                                                          >  SRR19973085.1578684/1‑150 (MQ=60)
                           GAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTGTTCGCTATGGTGACATAAATTCGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATACAGGGAAAATAACCGA                                                                                                                          >  SRR19973085.1578674/1‑150 (MQ=60)
                           GAAACGCAGACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTA                                                                                                                          <  SRR19973085.2892947/150‑1 (MQ=60)
                            AAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATATCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAA                                                                                                                         >  SRR19973085.4368444/1‑150 (MQ=60)
                            AAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTAGCTATGGTGACTTCAATTCGCATAATAGCCCCCAGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAA                                                                                                                         >  SRR19973085.265190/1‑150 (MQ=60)
                                 CATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACA                                                                                                                    <  SRR19973085.3228812/150‑1 (MQ=60)
                                    ACTGAATACGGCGGGAAAGGTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACA                                                                                                                 <  SRR19973085.717761/150‑1 (MQ=60)
                                        AATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAA                                                                                                             <  SRR19973085.3357782/150‑1 (MQ=60)
                                              GCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACG                                                                                                       <  SRR19973085.468478/150‑1 (MQ=60)
                                              GCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACG                                                                                                       <  SRR19973085.3122542/150‑1 (MQ=60)
                                                GGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCATTGGAGAATTTTTCGCTATGGTGTCTTAAATTCGCATAATTGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACTGGAAGCTACGAT                                                                                                     <  SRR19973085.2866669/150‑1 (MQ=60)
                                                GGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAT                                                                                                     >  SRR19973085.3324960/1‑150 (MQ=60)
                                                GGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTGTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCAGTTGAAAATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGAT                                                                                                     >  SRR19973085.3642160/1‑150 (MQ=60)
                                                 GGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTAGCGATGGTGACTTCAATTCGCATAATAGCCCCCAGGTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCGACGATT                                                                                                    >  SRR19973085.3577010/1‑150 (MQ=60)
                                                  GAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTT                                                                                                   <  SRR19973085.2111660/150‑1 (MQ=60)
                                                   AAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTT                                                                                                  <  SRR19973085.4139318/150‑1 (MQ=60)
                                                       TTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGGCAACAGGAAGCTACGATTTTTATT                                                                                              <  SRR19973085.716865/150‑1 (MQ=60)
                                                       TTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATT                                                                                              <  SRR19973085.2765257/150‑1 (MQ=60)
                                                         CGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAAACTAATGACAACAGGAAGCTACGATTTTTATTGT                                                                                            >  SRR19973085.1759123/1‑150 (MQ=60)
                                                          GCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTAGCTATGGTGACTTCAATTCGCATAATATCCCCCAGTTGAATATGCTGGTTATTTATACAGGTAAAAAAACCTAATGACAACAGGAAGCTACGATTTTTATTTTT                                                                                           >  SRR19973085.4353057/1‑150 (MQ=60)
                                                           CCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTT                                                                                          <  SRR19973085.3248677/150‑1 (MQ=60)
                                                           CCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTT                                                                                          >  SRR19973085.4507942/1‑150 (MQ=60)
                                                           CCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTT                                                                                          <  SRR19973085.902016/150‑1 (MQ=60)
                                                           CCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTT                                                                                          <  SRR19973085.3255540/150‑1 (MQ=60)
                                                            CAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATATCCCCCTGTTGAATATACTGGTTATTTATAAAGGTAAAATAACCTAATGACAACAGGAAGCGACGATTTTTATTGTTTA                                                                                         >  SRR19973085.2123723/1‑150 (MQ=60)
                                                            CAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTA                                                                                         <  SRR19973085.149713/150‑1 (MQ=60)
                                                            CAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTA                                                                                         <  SRR19973085.3490082/150‑1 (MQ=60)
                                                            CAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCAAATGACCACAGGAAGCTACGATTTTTATTGTTTA                                                                                         >  SRR19973085.523205/1‑150 (MQ=60)
                                                             AGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAA                                                                                        <  SRR19973085.1611667/150‑1 (MQ=60)
                                                              GCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC                                                                                       <  SRR19973085.2767572/150‑1 (MQ=60)
                                                              GCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC                                                                                       >  SRR19973085.4378316/1‑150 (MQ=60)
                                                                 AGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAAGGA                                                                                    >  SRR19973085.3133620/1‑150 (MQ=60)
                                                                 AGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAAACTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAAGGA                                                                                    >  SRR19973085.784944/1‑150 (MQ=60)
                                                                  GGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGAC                                                                                   <  SRR19973085.4135649/150‑1 (MQ=60)
                                                                    GCGTCAATAGCCCCAGCTGGCAATGGAGTAGTTTTCGATATGGTGACTTCACTTCGCATTATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCA                                                                                 <  SRR19973085.2789712/150‑1 (MQ=60)
                                                                     CGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAG                                                                                <  SRR19973085.2007917/150‑1 (MQ=60)
                                                                     CGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCGCCGATTTTTATTTTTTAACGGACCAG                                                                                >  SRR19973085.2524242/1‑150 (MQ=60)
                                                                     CGTCAATAGCCCCAGCTGGCAATGGAGAAGTGTTAGCGATGGTGAATTAAAATCGCATAATAGCCCCCAGTTTAATATTCTGGTTATTTATAAAAGTAAAATAAACTAAATACAACACGAAGCTACGAATTTTATTTTTTAAAGGACCAA                                                                                >  SRR19973085.4353881/1‑150 (MQ=51)
                                                                      GTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTGGCTATGGTGACTTCAATTCGCAAAATATCCCCCCGTTGAATATGCTGGTTATTTATAAAGGTAAAATAACCAAAATACAACAGGAAGCTACGATTTTTTTTTTTTAAAGGACCAGC                                                                               >  SRR19973085.3533964/1‑150 (MQ=60)
                                                                        CAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTTTTTAACGGACCAGCGT                                                                             >  SRR19973085.4488010/1‑150 (MQ=60)
                                                                        CAATAGCCCCAGCTGGCAATGGAGAAGTGTTCGCTATTGTGACTTCAATTCGCATAATATCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTAAGATTTTTATTGTTTAACGGACCAGCGT                                                                             >  SRR19973085.2121914/1‑150 (MQ=60)
                                                                         AATAGCCCCAGCTGGCAATGGAGAAGTTTTCGGTATGGTGACTTCAAATCGCATAATATCCCCCAGTTGTATATGCTGGTTATTTATAAAGGTAAAATAACCTAATTTCAACAGGAAGCTACGATTTTTATTGTTTTACGGACCAGCGTA                                                                            >  SRR19973085.1588290/1‑150 (MQ=60)
                                                                           TAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTAGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACC                                                                          >  SRR19973085.1006489/1‑150 (MQ=60)
                                                                           TAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTAGCATAATAGCCCCCAGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCAAATGACAACAGGAAGCTAAGATTTTTATTGTTTAACGGACCAGCGTACC                                                                          >  SRR19973085.2843970/1‑150 (MQ=60)
                                                                            AGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCG                                                                         <  SRR19973085.810494/150‑1 (MQ=60)
                                                                             GCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGT                                                                        <  SRR19973085.4416645/150‑1 (MQ=60)
                                                                              CCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTT                                                                       <  SRR19973085.3650375/150‑1 (MQ=60)
                                                                                  AGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTATAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCC                                                                   <  SRR19973085.3971368/150‑1 (MQ=60)
                                                                                    CTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTACCCG                                                                 >  SRR19973085.4234617/1‑150 (MQ=60)
                                                                                    CTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCG                                                                 <  SRR19973085.965288/150‑1 (MQ=60)
                                                                                    CTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCG                                                                 <  SRR19973085.4080480/150‑1 (MQ=60)
                                                                                       GCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTAGCATAATATCCCCCTGTTGAATATGCTGGTTATTTATAAAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTTTTTAACGGACCAGCGTACCGTTTCCCCGGCG                                                              >  SRR19973085.963046/1‑150 (MQ=60)
                                                                                          ATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGG                                                           <  SRR19973085.2165080/150‑1 (MQ=60)
                                                                                          ATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGG                                                           <  SRR19973085.1737087/150‑1 (MQ=60)
                                                                                            GGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAA                                                         <  SRR19973085.178077/150‑1 (MQ=60)
                                                                                              AGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATG                                                       <  SRR19973085.2400945/150‑1 (MQ=60)
                                                                                              AGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATG                                                       <  SRR19973085.3123089/150‑1 (MQ=60)
                                                                                              AGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATG                                                       <  SRR19973085.3123066/150‑1 (MQ=60)
                                                                                              AGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATG                                                       <  SRR19973085.4475701/150‑1 (MQ=60)
                                                                                              AGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATG                                                       <  SRR19973085.3123020/150‑1 (MQ=60)
                                                                                                         CTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTG                                            >  SRR19973085.4316342/1‑150 (MQ=60)
                                                                                                           ATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTC                                          <  SRR19973085.1474759/150‑1 (MQ=60)
                                                                                                           ATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATAACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTC                                          >  SRR19973085.273012/1‑150 (MQ=60)
                                                                                                               TGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCA                                      <  SRR19973085.571773/150‑1 (MQ=60)
                                                                                                               TGACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCA                                      >  SRR19973085.2470394/1‑150 (MQ=60)
                                                                                                               TGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCA                                      >  SRR19973085.4077603/1‑150 (MQ=60)
                                                                                                                GACTTCAATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAG                                     <  SRR19973085.4323497/150‑1 (MQ=60)
                                                                                                                    TCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAG                                 <  SRR19973085.1427521/150‑1 (MQ=60)
                                                                                                                      AATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTG                               >  SRR19973085.1406987/1‑150 (MQ=60)
                                                                                                                      AATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTG                               >  SRR19973085.1408200/1‑150 (MQ=60)
                                                                                                                      AATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTG                               >  SRR19973085.2153508/1‑150 (MQ=60)
                                                                                                                      AATTCGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTG                               >  SRR19973085.4680398/1‑150 (MQ=60)
                                                                                                                          CGCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGAT                           <  SRR19973085.4133526/150‑1 (MQ=60)
                                                                                                                          CGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGAT                           <  SRR19973085.3127833/150‑1 (MQ=60)
                                                                                                                           GCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATG                          <  SRR19973085.4092009/150‑1 (MQ=60)
                                                                                                                           GCATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATG                          <  SRR19973085.4254614/150‑1 (MQ=60)
                                                                                                                             ATAATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCT                        >  SRR19973085.340992/1‑150 (MQ=60)
                                                                                                                                ATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTC                     >  SRR19973085.1936599/1‑150 (MQ=60)
                                                                                                                                ATAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTC                     <  SRR19973085.2011016/150‑1 (MQ=60)
                                                                                                                                 TAGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCA                    <  SRR19973085.2562508/150‑1 (MQ=60)
                                                                                                                                  AGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAG                   >  SRR19973085.4279684/1‑150 (MQ=60)
                                                                                                                                  AGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAG                   >  SRR19973085.3709096/1‑150 (MQ=60)
                                                                                                                                  AGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAG                   >  SRR19973085.4454708/1‑150 (MQ=60)
                                                                                                                                  AGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAG                   >  SRR19973085.3709162/1‑150 (MQ=60)
                                                                                                                                  AGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAG                   >  SRR19973085.3709250/1‑150 (MQ=60)
                                                                                                                                  AGCCCCCTGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAG                   >  SRR19973085.1796138/1‑150 (MQ=60)
                                                                                                                                    CCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCA                 >  SRR19973085.3342174/1‑150 (MQ=60)
                                                                                                                                      CCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAAC               >  SRR19973085.3560464/1‑150 (MQ=60)
                                                                                                                                        CTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTCCGATTTTGATAGAATAACGGACCAGCGAACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCT             >  SRR19973085.3001964/1‑150 (MQ=60)
                                                                                                                                         TGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGACTGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTG            >  SRR19973085.4106183/1‑150 (MQ=60)
                                                                                                                                         TGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTG            >  SRR19973085.1370188/1‑150 (MQ=60)
                                                                                                                                         TGTTGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTG            >  SRR19973085.1370194/1‑150 (MQ=60)
                                                                                                                                            TGAATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTG         >  SRR19973085.2605213/1‑150 (MQ=60)
                                                                                                                                              AATATGCTGGTTATTTATACAGGTATAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCT       <  SRR19973085.536581/150‑1 (MQ=60)
                                                                                                                                              AATATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCT       >  SRR19973085.3625036/1‑150 (MQ=60)
                                                                                                                                                 ATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGACTGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTT    >  SRR19973085.1836211/1‑150 (MQ=60)
                                                                                                                                                 ATGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTT    >  SRR19973085.1837215/1‑150 (MQ=60)
                                                                                                                                                 ATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCCTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTT    >  SRR19973085.2436564/1‑150 (MQ=60)
                                                                                                                                                  TGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTC   <  SRR19973085.1064275/150‑1 (MQ=60)
                                                                                                                                                  TGCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTC   <  SRR19973085.3410589/150‑1 (MQ=60)
                                                                                                                                                   GCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCA  >  SRR19973085.3975192/1‑150 (MQ=60)
                                                                                                                                                   GCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCA  >  SRR19973085.3974302/1‑150 (MQ=60)
                                                                                                                                                   GCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCA  >  SRR19973085.3524261/1‑150 (MQ=60)
                                                                                                                                                   GCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCA  >  SRR19973085.3339611/1‑150 (MQ=60)
                                                                                                                                                   ACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCA  <  SRR19973085.1947606/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
GTACCGATACGTGGCCTTCATTATCAGGAAACGCATACTGAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCA  >  CP009273/1116817‑1117113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑