Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 235,010 G→T 12.6% L72I (CTT→ATT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273235,0100GT12.6% 719.8 / 19.1 332L72I (CTT→ATT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (157/114);  new base T (19/39);  total (179/153)
Fisher's exact test for biased strand distribution p-value = 7.49e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 356 total aligned reads displayed.

AGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  CP009273/234861‑235159
                                                                                                                                                     |                                                                                                                                                     
 gTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagt                                                                                                                                                      <  1:682148/150‑1 (MQ=255)
  tATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTGTTTTTGATTAAGAATGATTTTATTATagtaagta                                                                                                                                                     >  1:3648751/1‑150 (MQ=255)
   aTTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAgtaattaa                                                                                                                                                    >  1:4210174/1‑150 (MQ=255)
   aTTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAgtaagtaa                                                                                                                                                    >  2:4756509/1‑150 (MQ=255)
    ttGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTGTTTTTTGTTTAGAATGATTTTTTTATAGTAATTaaa                                                                                                                                                   >  1:1815071/1‑150 (MQ=255)
       aCATATCCTGTGACAAAAGAAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTTATAATTATTTTATTATAGTAATTAAAAtt                                                                                                                                                >  1:1451063/1‑150 (MQ=255)
              cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                         >  2:4343175/1‑150 (MQ=255)
              cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                         <  1:4018634/150‑1 (MQ=255)
                gtgACAAAAGGATCTATTATAGGTGCTTTTACGATATCTATTAGTAAAAATATAAGTGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGTTTTTATTATCGTAAGTAAAATTTCATGtata                                                                                                                                       <  1:907190/150‑1 (MQ=255)
                 tgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAATAATTATTTTATTATAGTAATTAAAATTAAATTAatat                                                                                                                                      >  1:4464256/1‑150 (MQ=255)
                  gACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAtt                                                                                                                                     <  2:1287881/150‑1 (MQ=255)
                      aaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaa                                                                                                                                 >  2:3069890/1‑150 (MQ=255)
                        aGGAGCTATAAAAGGTGCTATTACGATAGCTATTATTAAAAAAAAAAGAGTAAGATGTATAGTTATGTCTGTGGCGAAATTGACTACCTTCGTTATTTTTTATAATAATGATTTTATTTTAGAAAATAAAAATAAAAGAATAATTaaaaa                                                                                                                               >  1:2770166/1‑150 (MQ=255)
                          gAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATTATTTTATTATAGTAATTAAAATTAAAAGAATATTTAAAAAgg                                                                                                                             >  1:510576/1‑150 (MQ=255)
                          gAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAgg                                                                                                                             <  1:829927/150‑1 (MQ=255)
                           aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATCGTAAGTAAAAATACATGAATATTTAAAAAGGa                                                                                                                            >  1:4711728/1‑150 (MQ=255)
                              tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAATGaaaa                                                                                                                         >  2:2794917/1‑150 (MQ=255)
                               aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGaaaaa                                                                                                                        >  1:3252195/1‑149 (MQ=255)
                               aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                        <  1:2373031/150‑1 (MQ=255)
                               aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                        <  1:3429677/150‑1 (MQ=255)
                               aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                        <  1:3429497/150‑1 (MQ=255)
                                ttAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACg                                                                                                                       <  2:4079727/150‑1 (MQ=255)
                                 tAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGa                                                                                                                      <  2:3372094/150‑1 (MQ=255)
                                 tAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAAAATTTAAAAAGGAAAACGa                                                                                                                      >  2:4155816/1‑150 (MQ=255)
                                    tggTGATATTACGATAGATATTAGTAAAAATATAAGAGTTAGCTGTATTGTTTTGTCTGTGGCGAATTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACAt                                                                                                                   <  2:3635746/149‑1 (MQ=255)
                                    aGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAt                                                                                                                   <  1:196445/150‑1 (MQ=255)
                                         cTATTACGATAGCTATTAGTAAAAATATAATTGTTAGCTGTTTTGTTATGTCTGTGGCGAAATTTACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAc                                                                                                              <  2:662854/150‑1 (MQ=255)
                                               cGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGc                                                                                                        >  2:4266811/1‑150 (MQ=255)
                                                 aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAAACGAAGcac                                                                                                      >  2:3824847/1‑150 (MQ=255)
                                                   atatattatTAAAATTATATGAGTTTGCTTTATTGTTTTGTCTGTGGCTAAATTTACTACCTTCGTTTTTTTTATTAATAATTTTTTTTTTATCGTATTTAAAATTACTTGAATTTTTAAAAAGGAAAACGACATGAAACCGAAGCACAg                                                                                                    <  1:388806/147‑1 (MQ=255)
                                                       gttAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAATAATGATTTTATTATCGTAAGTAAAAATACAAGAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGaaaa                                                                                                >  1:1390027/2‑148 (MQ=255)
                                                       aTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTAAGTTAAATTACATGAATATTTAAAAATGAAAACGACATGTAACCGAAGCACAGAAta                                                                                                >  1:1998520/1‑149 (MQ=255)
                                                       aTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATc                                                                                                >  1:5281634/1‑150 (MQ=255)
                                                       aTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATc                                                                                                <  2:810451/150‑1 (MQ=255)
                                                             aaaaaTATAAGATTTAGTTGTTTTTTTTTTTGTTTGGCTAATTTTAATACCTTGTTTTTTTTTTTTAATAATGATTTTATTATCTTAATTAAAATTACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACAtt                                                                                          <  2:3129007/150‑1 (MQ=255)
                                                             aaaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAACGAAGCACAGAATCAACAtt                                                                                          >  2:3751204/1‑150 (MQ=255)
                                                               aaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTTAGAATGATTTTATTATCGTAAGTAAAAATACATGAATATTTAAAAAGGTAAAAGACATGAAAACCAAGCACAGAATCAACaaaat                                                                                        >  1:2904417/1‑146 (MQ=255)
                                                                  tataATATTTAGCTTTATTTTTATGTCTGGGCCTAAATTCACACCCTTCTTTTTTTTTATTAACAATTATTTTATTATCTAAATAAAAATTACATCAATATTAAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCa                                                                                     <  1:124689/150‑1 (MQ=255)
                                                                   ataAGAGTTAGCTTTATTTTTTTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGTTTTTATTATCGTAAGTAAATTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                    <  2:290405/150‑1 (MQ=255)
                                                                   ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACAGAAGCACAGAATCAACATTATACaa                                                                                    >  2:3007103/1‑150 (MQ=255)
                                                                    taAGAGTTAGCTGTTTTGTTTTTTCTGTGGCTAAATTGACTACCTTCTTTTTTTTTATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                   <  2:1487080/150‑1 (MQ=255)
                                                                     aaGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACAATCTCCAATc                                                                                  >  2:4649176/1‑150 (MQ=255)
                                                                        agTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGTTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTATCCAataata                                                                               >  2:1670769/1‑150 (MQ=255)
                                                                         gTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAAAATTTAAAAAGGAAAAAGACAAGAAACCGAAGCACAGAATCAACAATATCCAAACaaaa                                                                              >  2:2429485/1‑150 (MQ=255)
                                                                          ttAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATaaa                                                                             >  2:4352758/1‑150 (MQ=255)
                                                                          ttAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACAAGAAACCGAAGCACAGAATCAACATTCTCCAATCATaaa                                                                             >  2:1077717/1‑150 (MQ=255)
                                                                           tAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTGTTTTTGTTTAAGAATTTTTTTATTATATTAATTAAAAATAAATGAAAATTTAAAAATTAAAAAGACATGAAAACGAAGCACAGAATAAACAATCTACAAACATaaaa                                                                            >  1:2343735/1‑150 (MQ=255)
                                                                            aGCTGTTTTTTTATGTATGTGGCTAATTTGACTACCTTCTTTTTTTTGATTAAGATTTATTTTATTATCTTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAat                                                                           <  2:1919250/150‑1 (MQ=255)
                                                                            aGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAATAATTATTTTATTTTCGTAAGTAAAAATAAATGAATATTTAAAAATGAAAAAGAAAATAAACCGAAGCAAAGAATCAAAAATATCACATAATaaaaa                                                                           >  1:4105967/1‑149 (MQ=255)
                                                                               tGTATTTTTATGTATGTGGCGAATTTGACTACCTTCTTTTTTTTGATTAATAATTATTTTATTATCGTAAGTAAAATTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAtt                                                                        <  2:1998987/150‑1 (MQ=255)
                                                                                    tGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACAAGAATATTTAAAAATGAAAACGAAATGAAACCGAAGCACAGAATAAACAATCTCCAAAAATAAAATAtttaaat                                                                   >  1:3970245/1‑146 (MQ=255)
                                                                                    tGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGt                                                                   >  2:4578142/1‑150 (MQ=255)
                                                                                        aTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCAATAAAAAAATATTTACGTGGAg                                                               >  1:1765652/1‑150 (MQ=255)
                                                                                          gTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTAAGTGGAGCa                                                             >  2:2645274/1‑150 (MQ=255)
                                                                                            cTGTGGCGAAATTGACTACCTTCTTTTTTTTTATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                                           <  2:3167922/150‑1 (MQ=255)
                                                                                            cTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATTATTTTATTATAGTAAGTAAAATTAAAAGAATATTTAAAAATGAAAAAGACATGAAAACGAAGCACAGAATAAACAATATACAAAAATAAAATATTTACGGGGAGCAtt                                                           >  1:3182169/1‑150 (MQ=255)
                                                                                               tgGCTAAATTGACTACCTTCTTTTTTTTTATTAAGAATTATTTTATTATCTTAATTAAAATTAAATTAATATTAAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                        <  1:3304268/150‑1 (MQ=255)
                                                                                                ggCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAATGATTTTTTTATAGTAAGTAAAATTACATGAATATTT‑AAAAGGAAAACGACATGAAAACGAAGCACAGAATAAAAATTATCCAATCATAAAATATTTCCGTGGTGCATTttatt                                                      >  1:1563654/1‑150 (MQ=255)
                                                                                                ggCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttat                                                       <  2:1195605/150‑1 (MQ=255)
                                                                                                 gCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACAGAAGCACAGAAACAAAAATATACAATCATAAAATATTTACGTGGAGCATTttatt                                                      >  1:2206257/1‑150 (MQ=255)
                                                                                                 gCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                      >  1:4844334/1‑150 (MQ=255)
                                                                                                   gAAATTGACTACCTTGGTTTTTTTTATTAATATTTATTTTATTATCTTAATTAAAATTAAATTAATATTTAAAAAGTAACACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTtattat                                                    <  2:1809994/150‑1 (MQ=255)
                                                                                                    aaaTTTACTCCCTTCTTTTTTTTTATAAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                   <  2:1601592/150‑1 (MQ=255)
                                                                                                    aaaTTCACTACCTTTTTTTTTTTTATTAATATTTATTTTATTATCTTAATAAAAATTACATTAATATTAAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                   <  1:5017379/150‑1 (MQ=255)
                                                                                                     aaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATTAAACCGAAGCACAGAAACAACATTATCCAATCATAAAAAATTTACGTGGAGCATTTTattattt                                                  >  2:4460373/1‑149 (MQ=255)
                                                                                                     aaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAAATACATGAATATTTAAAAATGAAAAAGACATGAAACCGAAGCACAGAATAAACAATATACAATCATAAAATATTTACGTGGTGCATTTTATTATTg                                                  >  1:616991/1‑150 (MQ=255)
                                                                                                          aCTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCAAAAAATATTTCCGTGGAGCATTTTATTATTGAatat                                             >  2:4890015/1‑150 (MQ=255)
                                                                                                           cTACCTTCTTTTTTTTGATTAATAATGATTTTATTATCGTAATTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAAtata                                            <  1:1122006/150‑1 (MQ=255)
                                                                                                           cTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAAtata                                            <  2:3956479/150‑1 (MQ=255)
                                                                                                              catactttttttttATTAATAATTATTTTATTATCTTAATAAAAATAACATGAATATTTAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                         <  1:4671718/144‑1 (MQ=255)
                                                                                                               cTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAgg                                        >  2:5127194/1‑150 (MQ=255)
                                                                                                                ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                       >  1:3933977/1‑150 (MQ=255)
                                                                                                                    tttttttGATTAATAATGATTTTATTATCTTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                   <  1:4343152/150‑1 (MQ=255)
                                                                                                                      tttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACt                                 <  2:1759917/150‑1 (MQ=255)
                                                                                                                        ttttATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                               <  2:864281/150‑1 (MQ=255)
                                                                                                                           gATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGt                            >  1:2687835/1‑150 (MQ=255)
                                                                                                                           gATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGt                            <  2:221966/150‑1 (MQ=255)
                                                                                                                            aTTAAGAATGATGTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCggga                           >  2:2335038/1‑148 (MQ=255)
                                                                                                                              taataaTGATTTTATTATCTAAAGTAAATTTAAATTAATATTTAAAAATTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                         <  1:2069067/150‑1 (MQ=255)
                                                                                                                               aaGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa                        >  2:4689410/1‑150 (MQ=255)
                                                                                                                                aGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaa                       >  2:392563/1‑150 (MQ=255)
                                                                                                                                 gAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                      <  1:3074326/150‑1 (MQ=255)
                                                                                                                                 gAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                      >  1:15263/1‑150 (MQ=255)
                                                                                                                                   attaTTTTATTATCTAAAGAAAAATTAAATTAATATTAAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACa                    <  1:4593922/147‑1 (MQ=255)
                                                                                                                                   aTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACa                    >  1:4860866/1‑150 (MQ=255)
                                                                                                                                      aTTTTATTATCTTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                 <  2:551257/150‑1 (MQ=255)
                                                                                                                                      aTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                 >  2:493985/1‑150 (MQ=255)
                                                                                                                                          tattatCGTAAGTAAGATAACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAATTCCGTGGAGCATTTTATTATTGAATATAGAGGTATAACTCCGGTAAAAAACAAAGAAGc             >  2:2177725/1‑150 (MQ=255)
                                                                                                                                           tttttCGAAATTAAAATTAATTTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCa            <  1:2547176/146‑1 (MQ=255)
                                                                                                                                           attatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCa            >  1:4551775/1‑150 (MQ=255)
                                                                                                                                            ttatCTTAATTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt           <  2:1018298/150‑1 (MQ=255)
                                                                                                                                            ttatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt           >  1:4877541/1‑150 (MQ=255)
                                                                                                                                             tatCTTAAGTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt          <  2:3858456/150‑1 (MQ=255)
                                                                                                                                             tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt          >  1:3759641/1‑150 (MQ=255)
                                                                                                                                                cGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa       <  2:3568311/150‑1 (MQ=255)
                                                                                                                                                cGTAAGAAAAATTAAATTATTATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa       <  1:3380040/150‑1 (MQ=255)
                                                                                                                                                 gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt      >  1:4426670/1‑150 (MQ=255)
                                                                                                                                                 gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATACTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt      <  2:1342732/150‑1 (MQ=255)
                                                                                                                                                  tatgtaaAATTACTTGAATATTAAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATg     <  1:5322208/147‑1 (MQ=255)
                                                                                                                                                  taattaaAATTACATTAATATTTAAAAATTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATg     <  2:3315465/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  CP009273/234861‑235159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A