breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,884 C→A P21Q (CCG→CAG)  folA → type 3 dihydrofolate reductase
RA 49,910 T→G W30G (TGG→GGG)  folA → type 3 dihydrofolate reductase
RA 59,627 C→A intergenic (‑153/+60) BW25113_RS25400 ← / ← rluA transposase/bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA
JC 1,193,954 (AATGATGA)6→5 intergenic (‑261/‑190) BW25113_RS05920 ← / → lit hypothetical protein/cell death peptidase Lit
RA 1,314,400 C→T A414A (GCG→GCA trpD ← bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD
RA 1,613,378 T→C M1A (GTG→GCG) † marR → multiple antibiotic resistance transcriptional regulator MarR
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 4,062,410 G→T P76Q (CCG→CAG)  yihQ ← sulfoquinovosidase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356981 357571 591 12 [0] [5] 6 lacA galactoside O‑acetyltransferase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 269666 =NA (NA)39 (0.800) 28/246 0.4 100% intergenic (+2/+146) ykfC/BW25113_RS01325 reverse transcriptase domain‑containing protein/IS5‑like element IS5 family transposase
?NZ_CP009273 = 481436 0 (0.000)coding (220/648 nt) acrR multidrug efflux transporter transcriptional repressor AcrR
* ? NZ_CP009273 = 287112NA (NA)29 (0.590) 24/246 0.6 96.7% intergenic (‑55/‑99) BW25113_RS01405/BW25113_RS01410 IS1‑like element IS1B family transposase/hypothetical protein
?NZ_CP009273 1972826 = 1 (0.020)intergenic (‑372/‑408) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 360104 =99 (2.030)144 (2.950) 62/246 0.0 59.3% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 481433 =2 (0.040)42 (0.860) 26/246 0.5 95.5% coding (217/648 nt) acrR multidrug efflux transporter transcriptional repressor AcrR
?NZ_CP009273 = 571241 NA (NA)coding (1041/1068 nt) BW25113_RS02855 porin
* ? NZ_CP009273 = 104600236 (0.740)33 (0.680) 27/244 0.5 61.9% intergenic (+16/+417) BW25113_RS05135/cspH IS1‑like element IS1D family transposase/cold shock‑like protein CspH
?NZ_CP009273 3370649 = 5 (0.100)coding (131/639 nt) sspA stringent starvation protein A
* ? NZ_CP009273 1203246 =7 (0.140)29 (0.680) 28/214 0.3 79.4% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 9 (0.210)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 12032617 (0.140)26 (0.610) 18/214 0.8 77.5% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 9 (0.210)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 19728330 (0.000)43 (0.880) 33/246 0.3 100% intergenic (‑379/‑401) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 3577555 NA (NA)intergenic (+15/‑209) BW25113_RS17830/BW25113_RS25760 IS1‑like element IS1A family transposase/hypothetical protein
* ? NZ_CP009273 = 33706555 (0.100)71 (1.450) 47/246 0.1 93.4% coding (125/639 nt) sspA stringent starvation protein A
?NZ_CP009273 3576788 = NA (NA)coding (391/417 nt) BW25113_RS17825 YrhA family protein