breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 111,013 G→A 6.0% intergenic (+29/‑31) secA → / → mutT preprotein translocase subunit, ATPase/dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
RA 111,016 T→C 6.0% intergenic (+32/‑28) secA → / → mutT preprotein translocase subunit, ATPase/dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
RA 127,442 T→A 7.3% V583E (GTG→GAG)  aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 156,683 G→A 5.6% G67G (GGC→GGT yadN ← putative fimbrial‑like adhesin protein
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 323,062 A→G 7.8% E242G (GAG→GGG)  ykgF → ferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component
RA 357,778 C→T 7.4% intergenic (+324/+13) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 475,971 C→G 5.4% intergenic (+20/+11) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 475,972 C→G 5.4% intergenic (+21/+10) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 611,895 G→T 5.4% S200R (AGC→AGA fepA ← ferrienterobactin outer membrane transporter
RA 670,147 C→T 5.3% A142A (GCG→GCA nadD ← nicotinic acid mononucleotide adenylyltransferase, NAD(P)‑dependent
RA 755,790 G→A 74.8% W77* (TGG→TGA sdhD → succinate dehydrogenase, membrane subunit, binds cytochrome b556
RA 756,966 C→T 8.8% H354Y (CAC→TAC)  sdhA → succinate dehydrogenase, flavoprotein subunit
RA 857,576 T→C 5.6% intergenic (+21/+220) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein
RA 857,578 C→G 5.6% intergenic (+23/+218) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein
RA 857,580 G→A 5.6% intergenic (+25/+216) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein
RA 900,712 G→A 5.1% intergenic (‑137/+154) artJ ← / ← artM arginine ABC transporter periplasmic binding protein/arginine ABC transporter permease
RA 900,713 G→A 5.1% intergenic (‑138/+153) artJ ← / ← artM arginine ABC transporter periplasmic binding protein/arginine ABC transporter permease
RA 957,452 T→C 6.2% *231R (TGA→CGA)  ycaP → UPF0702 family putative inner membrane protein
RA 1,015,912 T→A 5.9% intergenic (+30/+40) rmf → / ← fabA ribosome modulation factor/beta‑hydroxydecanoyl thioester dehydrase
RA 1,038,557 C→T 6.0% A273V (GCC→GTC)  cbdA → cytochrome bd‑II oxidase, subunit I
RA 1,120,361 G→A 5.0% R76C (CGC→TGC)  solA ← N‑methyltryptophan oxidase, FAD‑binding
JC JC 1,196,376 IS1 (+) +8 bp 5.4% intergenic (+3/+484) icd → / ← ymfD isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase/e14 prophage; putative SAM‑dependent methyltransferase
RA 1,205,650:1 +A 5.8% coding (102/183 nt) ymfR → e14 prophage; uncharacterized protein
RA 1,286,787 G→A 5.7% intergenic (+261/+279) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,287,009 A→T 8.0% intergenic (+483/+57) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,411,903 T→C 5.2% N16D (AAC→GAC)  ttcA ← tRNA s(2)C32 thioltransferase, iron sulfur cluster protein
RA 1,708,854 C→G 20.5% Q646E (CAA→GAA)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,729,865 C→T 5.9% S260L (TCG→TTG)  lhr → putative ATP‑dependent helicase
RA 1,820,566 T→A 5.6% I217F (ATC→TTC)  chbC ← N,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS
RA 1,845,901 G→A 5.2% H354Y (CAT→TAT)  topB ← DNA topoisomerase III
RA 1,864,758 G→A 5.5% intergenic (+24/+24) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
RA 1,864,763 A→T 5.9% intergenic (+29/+19) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
RA 1,864,768 T→C 6.7% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
RA 1,933,049 A→G 5.4% V519A (GTG→GCG)  edd ← 6‑phosphogluconate dehydratase
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,009,731 A→G 6.0% intergenic (+19/‑90) yedF → / → yedK putative TusA family sulfurtransferase/DUF159 family protein
RA 2,009,732 C→G 6.7% intergenic (+20/‑89) yedF → / → yedK putative TusA family sulfurtransferase/DUF159 family protein
RA 2,009,733 C→T 5.8% intergenic (+21/‑88) yedF → / → yedK putative TusA family sulfurtransferase/DUF159 family protein
RA 2,016,877 G→C 5.1% K108N (AAG→AAC fliI → flagellum‑specific ATP synthase
RA 2,016,878 C→T 5.1% Q109* (CAG→TAG) ‡ fliI → flagellum‑specific ATP synthase
RA 2,016,879 A→G 5.1% Q109R (CAG→CGG) ‡ fliI → flagellum‑specific ATP synthase
RA 2,016,880 G→C 5.1% Q109H (CAG→CAC) ‡ fliI → flagellum‑specific ATP synthase
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,372,984 G→A 5.1% T31T (ACG→ACA arnF → undecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit
RA 2,383,956 A→T 6.0% intergenic (+32/+39) elaD → / ← yfbK protease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein
RA 2,383,959 A→T 6.6% intergenic (+35/+36) elaD → / ← yfbK protease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein
RA 2,416,337 G→A 5.5% A531T (GCA→ACA)  pta → phosphate acetyltransferase
RA 2,560,996 G→T 14.9% intergenic (+98/‑372) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
RA 2,586,450 C→A 6.4% L240L (CTC→CTA narQ → sensory histidine kinase in two‑component regulatory system with NarP
RA 2,716,030 T→G 6.4% intergenic (+20/+36) eamB → / ← grcA cysteine and O‑acetylserine exporter/autonomous glycyl radical cofactor
RA 2,716,033 C→A 6.4% intergenic (+23/+33) eamB → / ← grcA cysteine and O‑acetylserine exporter/autonomous glycyl radical cofactor
RA 2,767,667 T→G 5.7% intergenic (+312/‑43) rnlB → / → yfjP CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,767,670 C→A 7.4% intergenic (+315/‑40) rnlB → / → yfjP CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,802,411 C→A 5.0% A355E (GCA→GAA)  nrdE → ribonucleoside‑diphosphate reductase 2, alpha subunit
RA 2,804,528 C→A 11.1% intergenic (+67/‑287) nrdF → / → proV ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein
RA 2,866,487 G→C 6.3% intergenic (+22/+72) ygbN → / ← rpoS putative transporter/RNA polymerase, sigma S (sigma 38) factor
RA 2,866,488 G→C 6.6% intergenic (+23/+71) ygbN → / ← rpoS putative transporter/RNA polymerase, sigma S (sigma 38) factor
RA 2,951,471 G→A 7.0% R331C (CGC→TGC)  recD ← exonuclease V (RecBCD complex), alpha chain
RA 2,951,475 T→A 6.8% L329L (CTA→CTT recD ← exonuclease V (RecBCD complex), alpha chain
RA 2,951,479 T→C 6.9% Q328R (CAG→CGG)  recD ← exonuclease V (RecBCD complex), alpha chain
RA 3,139,632 A→G 5.2% intergenic (+39/+84) yghU → / ← hybG putative S‑transferase/hydrogenase 2 accessory protein
RA 3,139,633 A→C 5.3% intergenic (+40/+83) yghU → / ← hybG putative S‑transferase/hydrogenase 2 accessory protein
RA 3,139,639 T→G 6.2% intergenic (+46/+77) yghU → / ← hybG putative S‑transferase/hydrogenase 2 accessory protein
RA 3,165,428 C→T 5.2% P182P (CCG→CCA parC ← DNA topoisomerase IV, subunit A
RA 3,199,287 Δ1 bp 5.1% coding (355/2841 nt) glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
RA 3,199,288 Δ1 bp 5.1% coding (354/2841 nt) glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
RA 3,199,289 Δ1 bp 5.1% coding (353/2841 nt) glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
RA 3,270,030 T→C 24.7% intergenic (+428/+186) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,282,202 C→T 6.0% A76V (GCC→GTC)  agaS → tagatose‑6‑phosphate ketose/aldose isomerase
RA 3,439,589 G→C 6.2% intergenic (‑80/+27) yhdN ← / ← rplQ DUF1992 family protein/50S ribosomal subunit protein L17
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,683,472 G→T 9.1% intergenic (‑25/+158) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
RA 3,683,473 G→T 11.6% intergenic (‑26/+157) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
RA 3,683,479 Δ1 bp 14.3% intergenic (‑32/+151) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
RA 3,683,585 G→A 39.6% intergenic (‑138/+45) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
RA 3,786,146 C→T 6.3% T296I (ACC→ATC)  gpmM → phosphoglycero mutase III, cofactor‑independent
JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,166,239 A→G 5.7% Q271R (CAG→CGG)  murI → glutamate racemase
RA 4,177,171 T→A 6.9% intergenic (+43/‑187) tufB → / → secE translation elongation factor EF‑Tu 2/preprotein translocase membrane subunit
RA 4,177,172 T→A 6.4% intergenic (+44/‑186) tufB → / → secE translation elongation factor EF‑Tu 2/preprotein translocase membrane subunit
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,060 C→T 35.3% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,479,020 A→T 5.3% intergenic (+131/+14) rraB → / ← yjgM protein inhibitor of RNase E/GNAT family putative N‑acetyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 761486 762748 1263 40 [39] [38] 40 [sucA]–sucB [sucA],sucB
* * ÷ NC_000913 3423741–3424234 3424544–3424238 5–804 44 [39] [39] 41 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 224383NA (NA)3 (0.030) 3/256 NT NA noncoding (613/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?NC_000913 = 224395 NA (NA)noncoding (625/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
* ? NC_000913 = 357562NA (NA)3 (0.030) 3/250 NT NA noncoding (79/79 nt) REP27 (repetitive extragenic palindromic) element; contains 2 REP sequences REP27 (repetitive extragenic palindromic) element; contains 2 REP sequences
?NC_000913 = 357737 NA (NA)noncoding (54/79 nt) REP28 (repetitive extragenic palindromic) element; contains 2 REP sequences REP28 (repetitive extragenic palindromic) element; contains 2 REP sequences
* ? NC_000913 556631 =86 (0.920)5 (0.060) 4/246 NT 6.3% intergenic (‑78/+30) ybcI/ybcJ DUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
?NC_000913 556653 = 68 (0.780)intergenic (‑100/+8) ybcI/ybcJ DUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
* ? NC_000913 = 58076897 (1.040)5 (0.060) 5/232 NT 5.0% intergenic (‑323/‑66) ylcI/nohD DUF3950 family protein, DLP12 prophage/DLP12 prophage; DNA packaging protein
?NC_000913 = 580793 104 (1.270)intergenic (‑348/‑41) ylcI/nohD DUF3950 family protein, DLP12 prophage/DLP12 prophage; DNA packaging protein
* ? NC_000913 = 696409NA (NA)7 (0.080) 7/242 NT NA intergenic (+133/+21) ubiF/glnX 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase/tRNA‑Gln
?NC_000913 = 696413 NA (NA)intergenic (+137/+17) ubiF/glnX 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase/tRNA‑Gln
* ? NC_000913 = 75490987 (0.930)8 (0.090) 8/262 NT 9.8% intergenic (‑441/‑268) gltA/sdhC citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556
?NC_000913 760172 = 61 (0.660)coding (1467/2802 nt) sucA 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
* ? NC_000913 1207790 =28 (0.300)43 (0.520) 36/232 NT 57.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 38 (0.460)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780529 (0.310)30 (0.370) 30/232 NT 48.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 38 (0.460)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1211601 =113 (1.210)6 (0.070) 6/246 NT 5.5% intergenic (+24/+79) icdC/iraM pseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor
?NC_000913 1211624 = 99 (1.140)intergenic (+47/+56) icdC/iraM pseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor
* ? NC_000913 1237689 =73 (0.780)4 (0.040) 4/256 NT 5.5% coding (119/1299 nt) dadA D‑amino acid dehydrogenase
?NC_000913 1237731 = 67 (0.740)coding (161/1299 nt) dadA D‑amino acid dehydrogenase
* ? NC_000913 = 12994980 (0.000)140 (1.550) 98/256 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1451450 =84 (0.900)4 (0.050) 4/246 NT 5.1% intergenic (‑101/+147) tynA/paaZ tyramine oxidase, copper‑requiring/oxepin‑CoA hydrolase and 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
?NC_000913 1451475 = 70 (0.800)intergenic (‑126/+122) tynA/paaZ tyramine oxidase, copper‑requiring/oxepin‑CoA hydrolase and 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
* ? NC_000913 1985101 =79 (0.850)4 (0.050) 4/242 NT 5.3% intergenic (‑32/+38) araG/araF L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
?NC_000913 1985124 = 72 (0.840)intergenic (‑55/+15) araG/araF L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
* ? NC_000913 1997022 =95 (1.020)5 (0.060) 3/244 NT 5.3% intergenic (‑190/+40) sdiA/yecC quorum‑sensing transcriptional activator/putative ABC transporter ATPase
?NC_000913 1997051 = 91 (1.050)intergenic (‑219/+11) sdiA/yecC quorum‑sensing transcriptional activator/putative ABC transporter ATPase
* ? NC_000913 2297367 =81 (0.870)4 (0.040) 4/258 NT 5.2% coding (278/624 nt) ccmA heme export ABC transporter ATPase
?NC_000913 2297401 = 66 (0.720)coding (244/624 nt) ccmA heme export ABC transporter ATPase
* ? NC_000913 2652246 =108 (1.160)6 (0.070) 6/256 NT 5.5% coding (42/4962 nt) yfhM bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
?NC_000913 2652268 = 101 (1.110)coding (20/4962 nt) yfhM bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
* ? NC_000913 2729443 =37 (0.400)3 (0.030) 3/244 NT 8.0% noncoding (2/76 nt) gltW tRNA‑Glu
?NC_000913 2729485 = 35 (0.410)intergenic (‑41/+131) gltW/rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
* ? NC_000913 3150551 =NA (NA)4 (0.050) 3/242 NT NA intergenic (+5/+267) yghA/exbD putative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex
?NC_000913 3150580 = NA (NA)noncoding (27/255 nt) RIP222 (repetitive extragenic palindromic) element; contains 5 REP sequences and 2 IHF sites RIP222 (repetitive extragenic palindromic) element; contains 5 REP sequences and 2 IHF sites
* ? NC_000913 = 3427065NA (NA)4 (0.050) 4/246 NT NA intergenic (‑32/+11) alaU/ileU tRNA‑Ala/tRNA‑Ile
?NC_000913 = 3427068 NA (NA)intergenic (‑35/+8) alaU/ileU tRNA‑Ala/tRNA‑Ile
* ? NC_000913 = 352814285 (0.910)6 (0.070) 5/244 NT 6.8% coding (1674/2136 nt) yrfF putative RcsCDB‑response attenuator, inner membrane protein
?NC_000913 = 3528152 85 (0.980)coding (1684/2136 nt) yrfF putative RcsCDB‑response attenuator, inner membrane protein
* ? NC_000913 = 3943635NA (NA)4 (0.050) 4/250 NT 5.9% intergenic (+125/‑69) gltU/rrlC tRNA‑Glu/23S ribosomal RNA of rrnC operon
?NC_000913 = 4037446 67 (0.720)intergenic (+111/‑73) alaT/rrlA tRNA‑Ala/23S ribosomal RNA of rrnA operon
* ? NC_000913 = 420989315 (0.160)3 (0.040) 3/242 NT 17.9% intergenic (+44/‑150) gltV/rrlE tRNA‑Glu/23S ribosomal RNA of rrnE operon
?NC_000913 = 4209911 NA (NA)intergenic (+62/‑132) gltV/rrlE tRNA‑Glu/23S ribosomal RNA of rrnE operon
* ? NC_000913 4369146 =72 (0.770)5 (0.060) 4/244 NT 7.1% intergenic (+6/+10) fxsA/yjeH suppressor of F exclusion of phage T7/putative transporter
?NC_000913 4369185 = 65 (0.750)coding (1228/1257 nt) yjeH putative transporter