breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM2604801,408,0761.T33.3% 29.6 / 25.0 18coding (282/369 nt)h16_B12505‑Carboxymethyl‑2‑hydroxymuconate isomerase
*AM2604802,597,4461.G33.3% 10.0 / 12.6 12coding (668/1029 nt)h16_B2296transcriptional regulator, LysR‑family
*AM2604801,408,0710GA30.0% 30.9 / 13.8 20S96F (TCC→TTC) h16_B12505‑Carboxymethyl‑2‑hydroxymuconate isomerase
*AM2604801,408,0830A.30.0% 48.2 / 17.6 20coding (275/369 nt)h16_B12505‑Carboxymethyl‑2‑hydroxymuconate isomerase
*AM2604793,418,7190C.27.8% 41.8 / 13.6 19intergenic (+258/‑317)h16_A3158/corA2predicted permease of the drug/metabolite transporter (DMT) superfamily/Putative Mg2+ and Co2+ transporter
*AM2604793,418,7200AT27.8% 31.4 / 11.1 19intergenic (+259/‑316)h16_A3158/corA2predicted permease of the drug/metabolite transporter (DMT) superfamily/Putative Mg2+ and Co2+ transporter
*AM2604793,418,7220AT27.3% 40.1 / 14.7 22intergenic (+261/‑314)h16_A3158/corA2predicted permease of the drug/metabolite transporter (DMT) superfamily/Putative Mg2+ and Co2+ transporter
*AM2604793,418,7250AG27.3% 34.2 / 13.4 22intergenic (+264/‑311)h16_A3158/corA2predicted permease of the drug/metabolite transporter (DMT) superfamily/Putative Mg2+ and Co2+ transporter
*AM2604791,264,5340GC26.3% 34.0 / 12.0 19A325A (GCG→GCChscAmolecular chaperone (HscA)
*AM2604791,264,5350GC26.3% 34.8 / 12.1 19G326R (GGC→CGC) hscAmolecular chaperone (HscA)
*AM260480467,4131.C25.0% 27.8 / 12.0 16intergenic (+387/‑201)h16_B0409/h16_B0410Non‑functional due to fragmentation: Partial acyl‑CoA synthetase (AMP‑forming)/AMP‑acid ligase II/outer membrane protein (porin)
*AM2604793,418,7230AT23.8% 38.9 / 10.8 22intergenic (+262/‑313)h16_A3158/corA2predicted permease of the drug/metabolite transporter (DMT) superfamily/Putative Mg2+ and Co2+ transporter
*AM2604793,418,7241.T22.7% 53.2 / 18.6 22intergenic (+263/‑312)h16_A3158/corA2predicted permease of the drug/metabolite transporter (DMT) superfamily/Putative Mg2+ and Co2+ transporter
*AM2604793,346,8400CA20.0% 82.6 / 10.3 30L327M (CTG→ATG) dldD‑Lactate dehydrogenase (Cytochrome)

Marginal new junction evidence (lowest skew 10 of 171 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AM260480 2564349 =39 (1.280)5 (0.210) 4/202 1.4 18.2% coding (1361/3738 nt) narG2 respiratory nitrate reductase alpha chain (NarG)
?AY305378 288727 = 7 (0.390)coding (1384/3762 nt) narG respiratory nitrate reductase catalytic subunit
* ? AM260479 = 243724225 (0.940)6 (0.250) 6/188 1.5 19.2% coding (664/1029 nt) ssuA1 ABC‑type transporter, periplasmic component: TauT family
?AM260479 = 2437275 28 (1.160)coding (631/1029 nt) ssuA1 ABC‑type transporter, periplasmic component: TauT family
* ? AM260479 837552 =41 (1.530)6 (0.240) 6/198 1.5 13.6% coding (1224/1290 nt) dadA2 D‑amino‑acid dehydrogenase
?AM260479 837583 = 37 (1.450)coding (1255/1290 nt) dadA2 D‑amino‑acid dehydrogenase
* ? AM260480 = 284931436 (1.180)6 (0.240) 6/172 1.6 15.8% coding (35/1647 nt) h16_B2522 Medium‑chain‑fatty‑acid‑CoA ligase
?AM260480 = 2849296 34 (1.350)coding (17/1647 nt) h16_B2522 Medium‑chain‑fatty‑acid‑CoA ligase
* ? AM260480 = 29186624 (0.790)3 (0.180)
+47 bp
3/114 1.7 16.0% coding (734/2331 nt) flhF Flagellar GTP‑binding protein
?AM260480 291914 = 34 (1.110)coding (782/2331 nt) flhF Flagellar GTP‑binding protein
* ? AM260479 3775624 =26 (0.970)5 (0.200) 5/190 1.7 18.3% coding (175/522 nt) rplJ LSU ribosomal protein L10
?AM260479 3775644 = 21 (0.860)coding (155/522 nt) rplJ LSU ribosomal protein L10
* ? AM260479 1803401 =34 (1.270)6 (0.240) 5/192 1.7 14.9% coding (635/912 nt) h16_A1656 Ap4A phosphorylase II
?AM260479 1803438 = 37 (1.500)coding (672/912 nt) h16_A1656 Ap4A phosphorylase II
* ? AM260479 1002490 =20 (0.750)5 (0.200) 5/192 1.7 18.7% coding (455/1467 nt) cafA1 Ribonuclease G and E
?AM260479 1002530 = 25 (1.010)coding (415/1467 nt) cafA1 Ribonuclease G and E
* ? AM260479 = 59612028 (1.050)5 (0.200) 5/190 1.7 17.4% coding (939/1197 nt) pgk phosphoglycerate kinase
?AM260479 = 596225 22 (0.900)coding (1044/1197 nt) pgk phosphoglycerate kinase
* ? AM260480 772858 =28 (0.920)6 (0.210) 6/198 1.8 17.7% coding (1260/1389 nt) rtxD2 RTX toxin membrane fusion protein
?AM260480 772887 = 29 (1.000)coding (1289/1389 nt) rtxD2 RTX toxin membrane fusion protein