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breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AM260480 | 1,408,076 | 1 | . | T | 33.3% | 29.6 / 25.0 | 18 | coding (282/369 nt) | h16_B1250 | 5‑Carboxymethyl‑2‑hydroxymuconate isomerase |
* | AM260480 | 2,597,446 | 1 | . | G | 33.3% | 10.0 / 12.6 | 12 | coding (668/1029 nt) | h16_B2296 | transcriptional regulator, LysR‑family |
* | AM260480 | 1,408,071 | 0 | G | A | 30.0% | 30.9 / 13.8 | 20 | S96F (TCC→TTC) | h16_B1250 | 5‑Carboxymethyl‑2‑hydroxymuconate isomerase |
* | AM260480 | 1,408,083 | 0 | A | . | 30.0% | 48.2 / 17.6 | 20 | coding (275/369 nt) | h16_B1250 | 5‑Carboxymethyl‑2‑hydroxymuconate isomerase |
* | AM260479 | 3,418,719 | 0 | C | . | 27.8% | 41.8 / 13.6 | 19 | intergenic (+258/‑317) | h16_A3158/corA2 | predicted permease of the drug/metabolite transporter (DMT) superfamily/Putative Mg2+ and Co2+ transporter |
* | AM260479 | 3,418,720 | 0 | A | T | 27.8% | 31.4 / 11.1 | 19 | intergenic (+259/‑316) | h16_A3158/corA2 | predicted permease of the drug/metabolite transporter (DMT) superfamily/Putative Mg2+ and Co2+ transporter |
* | AM260479 | 3,418,722 | 0 | A | T | 27.3% | 40.1 / 14.7 | 22 | intergenic (+261/‑314) | h16_A3158/corA2 | predicted permease of the drug/metabolite transporter (DMT) superfamily/Putative Mg2+ and Co2+ transporter |
* | AM260479 | 3,418,725 | 0 | A | G | 27.3% | 34.2 / 13.4 | 22 | intergenic (+264/‑311) | h16_A3158/corA2 | predicted permease of the drug/metabolite transporter (DMT) superfamily/Putative Mg2+ and Co2+ transporter |
* | AM260479 | 1,264,534 | 0 | G | C | 26.3% | 34.0 / 12.0 | 19 | A325A (GCG→GCC) | hscA | molecular chaperone (HscA) |
* | AM260479 | 1,264,535 | 0 | G | C | 26.3% | 34.8 / 12.1 | 19 | G326R (GGC→CGC) | hscA | molecular chaperone (HscA) |
* | AM260480 | 467,413 | 1 | . | C | 25.0% | 27.8 / 12.0 | 16 | intergenic (+387/‑201) | h16_B0409/h16_B0410 | Non‑functional due to fragmentation: Partial acyl‑CoA synthetase (AMP‑forming)/AMP‑acid ligase II/outer membrane protein (porin) |
* | AM260479 | 3,418,723 | 0 | A | T | 23.8% | 38.9 / 10.8 | 22 | intergenic (+262/‑313) | h16_A3158/corA2 | predicted permease of the drug/metabolite transporter (DMT) superfamily/Putative Mg2+ and Co2+ transporter |
* | AM260479 | 3,418,724 | 1 | . | T | 22.7% | 53.2 / 18.6 | 22 | intergenic (+263/‑312) | h16_A3158/corA2 | predicted permease of the drug/metabolite transporter (DMT) superfamily/Putative Mg2+ and Co2+ transporter |
* | AM260479 | 3,346,840 | 0 | C | A | 20.0% | 82.6 / 10.3 | 30 | L327M (CTG→ATG) | dld | D‑Lactate dehydrogenase (Cytochrome) |
Marginal new junction evidence (lowest skew 10 of 171 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AM260480 | 2564349 = | 39 (1.280) | 5 (0.210) | 4/202 | 1.4 | 18.2% | coding (1361/3738 nt) | narG2 | respiratory nitrate reductase alpha chain (NarG) |
? | AY305378 | 288727 = | 7 (0.390) | coding (1384/3762 nt) | narG | respiratory nitrate reductase catalytic subunit | |||||
* | ? | AM260479 | = 2437242 | 25 (0.940) | 6 (0.250) | 6/188 | 1.5 | 19.2% | coding (664/1029 nt) | ssuA1 | ABC‑type transporter, periplasmic component: TauT family |
? | AM260479 | = 2437275 | 28 (1.160) | coding (631/1029 nt) | ssuA1 | ABC‑type transporter, periplasmic component: TauT family | |||||
* | ? | AM260479 | 837552 = | 41 (1.530) | 6 (0.240) | 6/198 | 1.5 | 13.6% | coding (1224/1290 nt) | dadA2 | D‑amino‑acid dehydrogenase |
? | AM260479 | 837583 = | 37 (1.450) | coding (1255/1290 nt) | dadA2 | D‑amino‑acid dehydrogenase | |||||
* | ? | AM260480 | = 2849314 | 36 (1.180) | 6 (0.240) | 6/172 | 1.6 | 15.8% | coding (35/1647 nt) | h16_B2522 | Medium‑chain‑fatty‑acid‑CoA ligase |
? | AM260480 | = 2849296 | 34 (1.350) | coding (17/1647 nt) | h16_B2522 | Medium‑chain‑fatty‑acid‑CoA ligase | |||||
* | ? | AM260480 | = 291866 | 24 (0.790) | 3 (0.180) +47 bp |
3/114 | 1.7 | 16.0% | coding (734/2331 nt) | flhF | Flagellar GTP‑binding protein |
? | AM260480 | 291914 = | 34 (1.110) | coding (782/2331 nt) | flhF | Flagellar GTP‑binding protein | |||||
* | ? | AM260479 | 3775624 = | 26 (0.970) | 5 (0.200) | 5/190 | 1.7 | 18.3% | coding (175/522 nt) | rplJ | LSU ribosomal protein L10 |
? | AM260479 | 3775644 = | 21 (0.860) | coding (155/522 nt) | rplJ | LSU ribosomal protein L10 | |||||
* | ? | AM260479 | 1803401 = | 34 (1.270) | 6 (0.240) | 5/192 | 1.7 | 14.9% | coding (635/912 nt) | h16_A1656 | Ap4A phosphorylase II |
? | AM260479 | 1803438 = | 37 (1.500) | coding (672/912 nt) | h16_A1656 | Ap4A phosphorylase II | |||||
* | ? | AM260479 | 1002490 = | 20 (0.750) | 5 (0.200) | 5/192 | 1.7 | 18.7% | coding (455/1467 nt) | cafA1 | Ribonuclease G and E |
? | AM260479 | 1002530 = | 25 (1.010) | coding (415/1467 nt) | cafA1 | Ribonuclease G and E | |||||
* | ? | AM260479 | = 596120 | 28 (1.050) | 5 (0.200) | 5/190 | 1.7 | 17.4% | coding (939/1197 nt) | pgk | phosphoglycerate kinase |
? | AM260479 | = 596225 | 22 (0.900) | coding (1044/1197 nt) | pgk | phosphoglycerate kinase | |||||
* | ? | AM260480 | 772858 = | 28 (0.920) | 6 (0.210) | 6/198 | 1.8 | 17.7% | coding (1260/1389 nt) | rtxD2 | RTX toxin membrane fusion protein |
? | AM260480 | 772887 = | 29 (1.000) | coding (1289/1389 nt) | rtxD2 | RTX toxin membrane fusion protein |