breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 85 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM1699,7220AG51.1% 112.1 / inf 931intergenic (+115/‑129)PP_t06 tRNA (tRNA‑Ala)/PP_23SD rRNA (23S ribosomal RNA)–/–
*AE015451_phaM11,327,1210CT50.3% 939.0 / inf 1721intergenic (+97/‑196)PP_16SE rRNA (16S ribosomal RNA)/PP_23SE rRNA (23S ribosomal RNA)–/–
*AE015451_phaM14,348,7050AG50.1% 1.3 / inf 1513intergenic (‑70/+404)PP_3820 CDS (Group II intron‑encoding maturase)/galU CDS (UTP‑glucose‑1‑phosphate uridylyltransferase)–/–
*AE015451_phaM14,344,3120GA47.8% 40.1 / 49.2 129intergenic (+43/‑113)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
*AE015451_phaM15,223,0130CA47.1% ‑6.2 / 28.8 51L428F (TTG→TTTPP_4603 CDS (transposase)
*AE015451_phaM14,675,3600TG46.5% 150.8 / 114.2 283intergenic (+59/+45)PP_4137 CDS (putative Outer membrane siderophore receptor)/chrR CDS (Chromate reductase)–/–
*AE015451_phaM14,344,3080GA42.0% 100.8 / 34.1 141intergenic (+39/‑117)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
*AE015451_phaM1195,6340CT36.1% 1657.1 / inf 1282T380T (ACC→ACTPP_0168 CDS (putative surface adhesion protein)
*AE015451_phaM15,222,9940GT35.6% ‑3.2 / 10.7 45L435M (CTG→ATG) PP_4603 CDS (transposase)
*AE015451_phaM15,737,7560AG33.2% 316.5 / 104.6 277intergenic (+45/+26)hutF CDS (probable formiminoglutamate deiminase)/PP_5037 CDS (lipocalin family lipoprotein)–/–
*AE015451_phaM15,097,2400AC32.1% 268.3 / 16.0 196intergenic (+231/+61)PP_mr66 ncRNA (P6)/acsA‑I CDS (acetyl‑CoA synthetase)–/–
*AE015451_phaM15,671,6530CT30.9% 406.9 / 34.5 278intergenic (+72/‑98)PP_4978 CDS (conserved protein of unknown function)/PP_4979 CDS (putative Periplasmic binding protein)–/–
*AE015451_phaM14,889,6230AG30.8% 250.7 / 29.0 162intergenic (+69/‑84)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
*AE015451_phaM12,178,5680GA30.7% 2466.9 / inf 1334T268T (ACG→ACAPP_1931 CDS (conserved protein of unknown function)
*AE015451_phaM14,889,6440GC30.2% 571.4 / 137.3 274intergenic (+90/‑63)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
*AE015451_phaM14,675,3360TG29.9% 317.0 / 22.2 243intergenic (+35/+69)PP_4137 CDS (putative Outer membrane siderophore receptor)/chrR CDS (Chromate reductase)–/–
*AE015451_phaM11,210,8490AC29.3% 1148.2 / 89.9 585*540G (TGA→GGA) PP_1060 CDS (putative Glutamate synthase_ large subunit)
*AE015451_phaM15,199,6560GT28.7% 121.6 / 30.9 130intergenic (+31/+42)PP_4579 CDS (Transcriptional regulator_ LysR family)/PP_4580 CDS (conserved protein of unknown function)–/–
*AE015451_phaM14,344,3230GA27.5% 353.1 / 19.6 194intergenic (+54/‑102)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
*AE015451_phaM14,344,2960AC27.4% 298.3 / 27.4 198intergenic (+27/‑129)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–

Marginal new junction evidence (lowest skew 10 of 298 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451_phaM1 4808174 =63 (0.070)321 (0.480) 46/200 4.4 59.1% intergenic (‑283/‑272) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
?AE015451_phaM1 = 5986211 398 (0.590)intergenic (+229/‑317) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–
* ? AE015451_phaM1 = 555560841 (0.050)296 (0.330)
+GCC
58/264 5.2 88.1% intergenic (‑84/+87) PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer) –/–
?AE015451_phaM1 5555609 = 41 (0.050)intergenic (‑85/+86) PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer) –/–
* ? AE015451_phaM1 = 2472115283 (0.310)115 (0.130) 49/256 6.4 22.1% intergenic (+19/+130) PP_2166 CDS (Anti‑anti‑sigma factor)/PP_2167 CDS (putative PhoD‑like phosphatase) –/–
?AE015451_phaM1 2472313 = 543 (0.630)coding (1783/1851 nt) PP_2167 CDS (putative PhoD‑like phosphatase)
* ? AE015451_phaM1 = 56814153 (0.000)262 (0.300)
+CGGG
38/262 9.1 98.9% intergenic (‑133/+71) PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein) –/–
?AE015451_phaM1 5681416 = 3 (0.000)intergenic (‑134/+70) PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein) –/–
* ? AE015451_phaM1 = 5201567356 (0.390)71 (0.080) 33/264 10.6 21.3% intergenic (‑53/‑127) PP_4582 CDS (conserved membrane protein of unknown function)/PP_4583 CDS (putative Peptidase) –/–
?AE015451_phaM1 5201666 = 177 (0.200)intergenic (‑152/‑28) PP_4582 CDS (conserved membrane protein of unknown function)/PP_4583 CDS (putative Peptidase) –/–
* ? AE015451_phaM1 = 652782716 (0.790)97 (0.130) 25/230 11.4 24.1% coding (1961/1962 nt) ctpL CDS (Methyl‑accepting chemotaxis protein CtpL)
?AE015451_phaM1 = 4808159 1 (0.000)intergenic (‑268/‑287) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
* ? AE015451_phaM1 = 5699642910 (1.000)56 (0.010) 25/248 12.3 11.8% intergenic (+388/‑3617) PP_5002 CDS (conserved protein of unknown function)/PP_5009 CDS (conserved protein of unknown function) –/–
?pS4413_qbsFH 12 = 0 (0.000)intergenic (–/‑1941) –/aadA CDS –/–
* ? AE015451_phaM1 = 480817656 (0.060)42 (0.050) 25/254 12.7 18.0% intergenic (‑285/‑270) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
?AE015451_phaM1 4808280 = 329 (0.380)intergenic (‑389/‑166) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
* ? AE015451_phaM1 2478409 =996 (1.100)32 (0.040) 19/218 13.0 3.9% coding (106/444 nt) PP_2172 CDS (conserved exported protein of unknown function)
?AE015451_phaM1 = 2478405 776 (1.060)coding (102/444 nt) PP_2172 CDS (conserved exported protein of unknown function)
* ? AE015451_phaM1 = 5986033534 (0.590)45 (0.050) 23/254 13.4 8.4% intergenic (+51/‑495) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–
?AE015451_phaM1 5986255 = 477 (0.560)intergenic (+273/‑273) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–