breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG42.1% 381.6 / 82.9 260intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC35.1% 449.2 / 62.2 229G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP012306644,4170GA34.3% 651.1 / inf 530intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123061,226,5570AG30.6% 353.8 / 89.2 186G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP012306202,4170TG29.8% 548.3 / 40.1 257V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123061,421,1470TG29.1% 488.4 / 11.4 245T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
*AP0123063,527,3680AC27.1% 615.0 / 46.8 251L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306800,6750AC26.8% 509.8 / 25.3 213T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123062,680,3450AC25.7% 465.4 / 41.7 207T184P (ACC→CCC) ttdAL‑tartrate dehydratase, alpha subunit
*AP0123062,758,9090TG25.2% 588.5 / 28.9 226G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123063,590,3670AC25.2% 745.7 / 38.4 283T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
*AP012306406,6770AG24.4% 492.6 / 14.0 197K294E (AAA→GAA) hemHferrochelatase
*AP0123061,854,8510AC24.0% 768.2 / 55.0 296T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123063,182,8610AC23.6% 327.4 / 17.2 123L198F (TTA→TTCyiaNpredicted transporter
*AP0123062,212,7530AC23.1% 431.8 / 39.1 161Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP0123063,938,8870CT22.3% 545.2 / 95.9 224S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123062,560,2430TG22.3% 493.7 / 23.8 203N419T (AAC→ACC) pepPproline aminopeptidase P II
*AP0123063,324,4330TA21.9% 642.6 / 94.3 251intergenic (+2/+52)yieF/yieGchromate reductase, Class I, flavoprotein/predicted inner membrane protein
*AP0123062,714,5550TG21.9% 706.3 / 20.2 279T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP0123061,393,3790AC21.9% 672.5 / 36.8 238T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase

Marginal new junction evidence (lowest skew 10 of 141 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 997497337 (1.070)11 (0.040) 11/268 13.3 3.8% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 252 (0.890)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 215364NA (NA)10 (0.030) 10/288 14.1 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 1092512377 (1.200)9 (0.030) 8/258 14.2 2.8% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 289 (1.060)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 1906320321 (1.020)8 (0.030) 8/292 15.0 2.5% coding (692/1689 nt) yfaA predicted protein
?AP012306 = 1906331 321 (1.040)coding (681/1689 nt) yfaA predicted protein
* ? AP012306 = 2262152449 (1.430)7 (0.020) 7/290 15.4 1.6% intergenic (‑76/‑229) yfiD/ung pyruvate formate lyase subunit/uracil‑DNA‑glycosylase
?AP012306 = 2262160 449 (1.470)intergenic (‑84/‑221) yfiD/ung pyruvate formate lyase subunit/uracil‑DNA‑glycosylase
* ? AP012306 2166807 =266 (0.850)7 (0.020) 7/294 15.4 2.6% intergenic (‑308/‑17) upp/purM uracil phosphoribosyltransferase/phosphoribosylaminoimidazole synthetase
?AP012306 2166823 = 258 (0.830)intergenic (‑324/‑1) upp/purM uracil phosphoribosyltransferase/phosphoribosylaminoimidazole synthetase
* ? AP012306 1370757 =335 (1.070)6 (0.020) 6/270 15.4 1.9% coding (599/1176 nt) manA mannose‑6‑phosphate isomerase
?AP012306 1370771 = 308 (1.080)coding (613/1176 nt) manA mannose‑6‑phosphate isomerase
* ? AP012306 = 1092334349 (1.110)6 (0.020) 5/258 15.7 2.0% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 289 (1.060)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 3026675 =342 (1.090)6 (0.020) 6/290 15.8 1.7% coding (130/1104 nt) asd aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding
?AP012306 3026691 = 343 (1.120)coding (114/1104 nt) asd aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding
* ? AP012306 = 214142NA (NA)6 (0.020) 6/288 15.8 NA noncoding (1511/1542 nt) rrsH 16S ribosomal RNA (rrsH)
?AP012306 = 214154 NA (NA)noncoding (1523/1542 nt) rrsH 16S ribosomal RNA (rrsH)